清洁具有包含脂肪酸的污垢的表面的方法以及用于该方法的消费产品组合物
序列表的引用
本申请包含计算机可读形式的序列表。所述计算机可读形式以引用方式并入本文。
技术领域
本发明涉及将包含脂肪酸的污垢转化为活性脂肪酸衍生物物质的方法以及包含表面活性剂和污垢转化酶的消费产品组合物。
背景技术
消费产品组合物，例如用于清洁表面的那些，应具有良好的起泡特征，尤其是在油脂污垢的存在下具有持久的起泡特征，同时提供良好的污垢和/或油脂清洁性。使用者通常将泡沫视为洗涤剂组合物性能的指标。此外，洗涤剂组合物的使用者还可使用起泡特征和泡沫的外观(例如密度、白度)作为洗涤溶液仍含有活性洗涤剂成分的指示剂。因此，希望洗涤剂组合物在洗涤剂与水的初始混合期间和/或整个洗涤操作期间提供“良好的起泡特征”，该起泡特征包括良好的泡沫高度和/或密度以及良好的泡沫持续时间。
已经发现，某些类型的污垢，特别是包含脂肪酸的油脂污垢，可起到抑泡剂的作用，从而引发消费者比所需的更频繁地更换产品。因此，需要提供具有所需泡沫性能，尤其是在油脂污垢存在下，甚至是在包含脂肪酸的油脂污垢存在下，并且同时提供良好的污垢和油脂去除性质的洗涤剂组合物。
还期望在消费产品组合物中使用较少的表面活性剂物质。然而，使用较少的表面活性剂可能会降低消费产品组合物的泡沫生成和/或清洁性能。
仍然希望提供用于清洁具有包含脂肪酸的污垢的表面的消费产品组合物，该消费产品组合物提供有效的泡沫生成和/或清洁性能，尤其是当消费产品组合物在组合物中包含相对低量的表面活性剂时。
发明内容
本发明涉及一种清洁上面存在包含脂肪酸的污垢的表面的方法，该方法包括使该污垢与消费产品组合物接触，该消费产品组合物包含表面活性剂和污垢转化酶，该污垢转化酶选自氢过氧脂肪酸生成酶、氢过氧脂肪酸转化酶、多结构域酶、羟基脂肪酸生成酶、以及它们的混合物。该方法还包括将污垢的脂肪酸转化为选自氢过氧脂肪酸、氢过氧脂肪酸衍生物、羟基脂肪酸、以及它们的混合物的活性脂肪酸衍生物物质。
本发明还涉及消费产品组合物，该消费产品组合物包含：表面活性剂，该表面活性剂选自阴离子表面活性剂、非离子表面活性剂、两性离子表面活性剂、两性表面活性剂、阳离子表面活性剂、以及它们的混合物；和污垢转化酶，该污垢转化酶选自氢过氧脂肪酸生成酶、氢过氧脂肪酸转化酶、多结构域酶、羟基脂肪酸生成酶、以及它们的混合物。
具体实施方式
定义
如本文所用，当用于权利要求中时，冠词“一个”和“一种”被理解为是指受权利要求书保护或描述的一种或多种。
如本文所用，术语“大体上不含”是指按组合物的重量计，所指示的物质以不超过约5重量％，优选不超过约2％，并且更优选不超过约1重量％的量存在。
如本文所用，术语“基本不含”是指按组合物的重量计，所指示的物质以不超过约0.1重量％的量存在，或优选地不以分析上可检测的水平存在于此组合物中。其可包括这样的组合物，其中所指示的物质仅作为有意加入此类组合物中的一种或多种物质的杂质。
如本文所用，术语“洗涤剂组合物”是指设计用于清洁脏污表面的组合物或制剂。此类组合物包括但不限于盘碟洗涤组合物、衣物洗涤剂组合物、织物软化组合物、织物增强组合物、织物清新组合物、衣物洗涤预洗剂、衣物洗涤预处理剂、衣物洗涤添加剂、喷雾产品、干洗剂或组合物、衣物洗涤冲洗添加剂、洗涤添加剂、后冲洗织物处理剂、熨烫助剂、硬质表面清洁组合物、单位剂量制剂、延迟递送制剂、在多孔基底或非织造片材上或之内包含的洗涤剂、以及根据本文的教导内容对本领域的技术人员显而易见的其他合适的形式。此类组合物可被用作清洁预处理剂、清洁后处理剂，或也可在清洁过程的冲洗循环或洗涤循环期间添加。洗涤剂组合物可具有选自以下的形式：液体、粉末、单相或多相单位剂量或小袋形式、片剂、凝胶、糊剂、棒或薄片。优选地，组合物用于手动洗涤。优选地，本发明的洗涤剂组合物为盘碟洗涤剂。优选地，组合物为液体形式。
如本文所用，术语“片段”是指参考序列的至少30、60、100、150(或其间的任何整数)个连续氨基酸的氨基酸序列。
本文所用术语“同一性”是指两个或更多个序列之间的同一性，并且以序列之间的同一性或相似性来表示，该同一性或相似性如通过将序列的全长与参考序列的全长进行比对来计算。序列同一性可以同一性百分比来量度；百分比越高，序列同一性越高。同一性百分比通过比较的长度来计算。例如，通常通过将序列的全长与参考序列的全长进行比对来计算同一性。用于比较的序列比对方法是本领域熟知的，并且同一性可以通过许多已知方法来计算。本领域中描述了各种程序和比对算法。应当注意，术语“序列同一性”和“序列相似性”可以互换使用。
如本文所用，术语“增加的泡沫寿命”是指在使用包含一种或多种氢过氧脂肪酸生成酶和一种或多种氢过氧脂肪酸转化酶的组合物清洁脏污制品的洗涤过程中，与不存在氢过氧脂肪酸生成酶和氢过氧脂肪酸转化酶的情况下由相同组合物和方法提供的泡沫寿命相比，可见泡沫的持续时间增加。
如本文所用，术语“脏污表面”非特定地指任何类型的由天然纤维或人造纤维的网络构成的柔性材料，包括天然纤维、人造纤维和合成纤维，诸如但不限于棉、亚麻布、羊毛、聚酯、尼龙、丝绸、丙烯酸类等、以及各种共混物和组合。脏污表面还可指任何类型的硬质表面，包括天然表面、人造表面或合成表面，诸如但不限于砖材、花岗石、灰浆、玻璃、复合材料、乙烯树脂、硬木、金属、烹饪表面、塑料等、以及共混物和组合。本申请的主要目标脏污表面是脏污盘碟。
如本文所用，术语氢过氧脂肪酸生成酶或氢过氧脂肪酸转化酶的“变体”是指当氢过氧脂肪酸生成酶或氢过氧脂肪酸转化酶被选自取代、插入、缺失及其组合的一个或多个氨基酸修饰或在这些氨基酸处被修饰(例如1、2、3、4、5、6、7、8、9或10个或更多个氨基酸修饰)时的氨基酸序列。变体可以具有“保守”取代，其中被取代的氨基酸具有与取代它的氨基酸相似的结构或化学性质，例如用异亮氨酸替代亮氨酸。变体可以具有“非保守”改变，例如，用色氨酸替代甘氨酸。变体还可以包括具有氨基酸缺失或插入或两者的序列。使用本领域熟知的计算机程序可以找到确定哪些氨基酸残基可以被取代、插入或缺失而不消除蛋白质活性的指导。变体还可以包括衍生自野生型氢过氧脂肪酸生成酶或氢过氧脂肪酸转化酶的截短形式，例如具有截短的N-末端的蛋白质。变体还可以包括通过向野生型蛋白质添加额外的氨基酸序列，例如N-末端标签、C-末端标签或在蛋白序列中间的插入物而衍生的形式。
如本文所用，术语“水硬度”或“硬度”是指存在于水中的未络合阳离子(即，Ca2+或Mg2+)，这些阳离子在碱性条件下可能与阴离子表面活性剂或任何其他带阴离子电荷的洗涤剂活性物质一起沉淀，从而削弱表面活性剂的表面活性和清洁能力。另外，术语“高水硬度”或“提升的水硬度”可互换使用，并且出于本发明的目的，是相关术语，并且旨在包括但不限于包含至少12克钙离子每加仑水(gpg，“美国格令硬度”单位)的硬度水平。
消费产品组合物
本发明涉及一种包含表面活性剂和污垢转化酶的消费产品组合物。当用于接触上面存在包含脂肪酸的污垢的脏污表面时，所述消费产品组合物可将污垢的脂肪酸转化为活性脂肪酸衍生物物质。就这一点而言，在消费产品组合物中使用较低含量的表面活性剂时，本发明的消费产品组合物可表现出改善的清洁性能，或等同的清洁性能。
本发明的消费产品组合物包括但不限于用于下列的组合物：处理毛发(人类、狗和/或猫)，包括漂白、着色、染色、调理、生长、除去、延迟生长、用洗发剂洗发、定型；除臭剂和止汗剂；个人清洁；与处理皮肤(人、犬和/或猫)相关的产品和/或方法，所述处理包括施用乳霜、洗剂、以及消费者使用的其他局部施用的产品；剃刮；身体喷雾剂；用于处理织物、硬质表面以及任何其他在织物和居家护理领域中的表面的组合物，包括：空气护理、汽车护理、餐具洗涤、织物调理(包括软化)、衣物洗涤去垢、衣物洗涤和冲洗添加剂和/或护理、硬质表面清洁和/或处理、和用于消费者或企业使用的其他清洁；掺入与一次性吸收制品和/或非吸收制品相关的产品中的组合物，这些产品包括成人失禁衣服、围兜、尿布、训练裤、婴儿和幼儿护理擦拭物；洗手液、洗发剂、洗剂、口腔护理工具；诸如湿或干的卫生纸、面巾纸、一次性手帕、一次性毛巾和/或擦拭物的产品；掺入与月经垫、失禁衬垫、阴唇间衬垫、卫生护垫、阴道栓剂、卫生巾、棉塞和棉塞施用装置和/或擦拭物的产品中的组合物。在优选的方面，消费产品组合物为洗涤剂组合物。
本文中优选的消费产品组合物包括织物清洁组合物、硬表面清洁组合物、盘碟洗涤组合物和毛发清洁组合物。此类组合物通常包含一种或多种消费产品助剂成分。
优选的消费产品组合物为手动盘碟洗涤组合物，优选液体形式。其通常包含按所述组合物的重量计30％至95％，优选40％至90％，更优选50％至85％的液体载体，其他必要组分和任选组分溶解、分散或悬浮在所述液体载体中。液体载体的一个优选组分是水。
优选地，将本发明的消费产品组合物的pH调节至在3至14之间，更优选在4至13之间，更优选在6至12之间，并且最优选在8至10之间，所述pH在20℃下以在去离子水中的10％产品浓度测量。可使用本领域已知的pH调节成分来调节消费产品组合物的pH。
污垢转化酶
消费产品组合物包含：a)一种或多种污垢转化酶，其选自氢过氧脂肪酸生成酶、氢过氧脂肪酸转化酶、氢过氧脂肪酸生成和转化酶、过加氧酶、脂肪酸水合酶、以及它们的混合物；和b)表面活性剂。所述消费产品组合物还可包含如本文所述的消费产品助剂成分。
在一个方面，所述消费产品组合物中表面活性剂的含量按所述组合物的重量计小于约2％，优选小于约1％，优选小于约0.5％，优选小于约0.1％，或优选0％，同时通过将油性和/或油脂污垢转化为表面活性剂而仍然提供增强的对此类污垢的起泡和/或清洁有益效果。据信，起泡和/或清洁有益效果的增加是由于脂肪酸转化为更含氧的脂肪酸，表面活性剂性质增强，并且在硬水存在下沉淀的趋势减小。此外，据信表面活性剂的浓度按所述组合物的重量计低于约2％可有利于消费产品组合物中酶的稳定性和活性。
氢过氧脂肪酸生成酶
在分子氧(O2)存在下脂肪酸可以被氢过氧脂肪酸生成酶如双加氧酶氧化，以产生氢过氧脂肪酸。这些氢过氧化化合物可进一步被酶促处理或自发地转化为不同组的含氧脂肪酸和其他衍生物。在本申请的上下文中，“氢过氧脂肪酸生成酶”是能够将至少一种脂肪酸转化为含氧化合物的混合物的酶，该混合物至少包含作为中间体或作为最终产物的氢过氧脂肪酸。氢过氧脂肪酸生成酶的非限制性实例为脂氧合酶、α-双加氧酶、二醇合酶和含有具有双加氧酶(DOX)活性的结构域的其他酶。
在本发明的一个实施方案中，所述清洁组合物包含脂氧合酶。脂氧合酶(EC1.13.11.-)是一类(非血红素)、含铁或锰的双加氧酶(DOX)，其催化分子氧插入不饱和脂肪酸中以生成相应的氢过氧脂肪酸。本发明包括不同组的脂氧合酶，包括亚油酸脂氧合酶、花生四烯酸脂氧合酶和油酸脂氧合酶。尽管亚油酸脂氧合酶、花生四烯酸脂氧合酶和油酸脂氧合酶通常分别识别亚油酸/亚油酸酯、花生四烯酸/花生四烯酸酯以及油酸/油酸酯作为优选底物，但术语“亚油酸脂氧合酶”、“花生四烯酸脂氧合酶”和“油酸脂氧合酶”在本文中可互换使用，且不暗示任何底物特异性，即，相应的酶可作用于这些底物中的任一种。
区域特异性脂氧合酶催化不饱和脂肪酸的位置特异性氢过氧化。例如，动物12-和15-脂氧合酶和微生物15-脂氧合酶将花生四烯酸转化为对应的12-和15-氢过氧脂肪酸；而来自珊瑚和海胆的11-脂氧合酶产生11-氢过氧脂肪酸作为中间体或最终产物。类似地，植物和细菌的9-和13-脂氧合酶和真菌11-和13-脂氧合酶将亚油酸转化为其9-、11-和13-氢过氧脂肪酸衍生物。此外，一些双加氧酶能够催化分子氧在不饱和脂肪酸的若干位置处的结合。
作为本发明一部分的脂氧合酶的非限制性实例包括表1中所列的野生型脂氧合酶及其表现出脂氧合酶活性的变体。优选的脂氧合酶表现出与表1中所列野生型脂氧合酶中的一种或多种至少20％，优选至少30％，优选至少40％，优选至少50％，优选至少60％，优选至少70％，优选至少80％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性。
表1：脂氧合酶
在本发明的另一个实施方案中，所述清洁组合物包含α-双加氧酶。α-双加氧酶是另一类双加氧酶(DOX)，其通过立体选择性双加氧作用将饱和与不饱和脂肪酸转化为其对应的2-氢过氧脂肪酸。所得氢过氧脂肪酸可以自发脱羧为较短的醛。α-双加氧酶与脂氧合酶的不同之处在于在氧化过程中不需要不饱和碳键。α-双加氧酶通常由不同种类的植物和真菌编码，其中它们在针对病原体攻击的宿主防御反应期间被上调，但在细菌中也发现同源物。
作为本发明一部分的α-双加氧酶的非限制性实例包括表2中所列的野生型及其表现出α-双加氧酶活性的变体。优选的α-双加氧酶表现出至少20％，优选至少30％，优选至少40％，优选至少50％，优选至少60％，优选至少70％，优选至少80％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性，如通过将序列的全长与选自表2中所列野生型α-双加氧酶的至少一个参考序列的全长进行比对所计算的。
表2：α-双加氧酶
氢过氧脂肪酸转化酶
氢过氧脂肪酸可被催化或自发地转化为含氧衍生物，包括羟基-、二羟基-、氧代-、环氧-和酮脂肪酸、二乙烯基醚和醛(Andreou,A.等人(2009)，Prog.Lipid Res.48(3-4):148-170)。本发明的清洁组合物还可包含一种或多种氢过氧脂肪酸转化酶，所述氢过氧脂肪酸转化酶能够将氢过氧脂肪酸转化为氢过氧脂肪酸的一种或多种衍生物。优选地，氢过氧脂肪酸转化酶选自：环加氧酶(EC 1.14.99.1)、丙二烯氧化物合酶(EC 4.2.1.92)、氢过氧化物异构酶(EC 4.2.1.92、EC 5.3.99.1、EC 5.4.4.5、EC 5.4.4.6)、氢过氧化物裂解酶(EC 4.2.1.92)、氢过氧化物脱水酶(EC 4.2.1.92)、二乙烯基醚合酶(EC 4.2.1.121、EC4.2.1.B8、EC 4.2.1.B9)、9,12-十八碳二烯酸8-氢过氧化物8R-异构酶(EC 5.4.4.5)、9,12-十八碳二烯酸8-氢过氧化物8S-异构酶(EC 5.4.4.6)、7,10-氢过氧化物二醇合酶、环氧醇合酶、以及它们的混合物。优选地，氢过氧脂肪酸转化酶选自：氢过氧化物异构酶(EC4.2.1.92、EC 5.3.99.1、EC 5.4.4.5、EC 5.4.4.6)、氢过氧化物裂解酶(EC 4.2.1.92)、氢过氧化物脱水酶(EC 4.2.1.92)、7,10-氢过氧化物二醇合酶、环氧醇合酶、以及它们的混合物，优选7,10-氢过氧化物二醇合酶。
氢过氧脂肪酸转化酶的非限制性实例包括表2中列出的野生型及其表现出氢过氧脂肪酸转化酶活性的变体。优选的氢过氧脂肪酸转化酶表现出与表3中所列野生型中的一种或多种至少20％，优选至少30％，优选至少40％，优选至少50％，优选至少60％，优选至少70％，优选至少80％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性。优选的氢过氧脂肪酸转化酶也可以是表3中所列野生型的片段(例如N-末端结构域或C-末端结构域)。
表3：氢过氧脂肪酸转化酶
由氢过氧脂肪酸转化酶转化氢过氧脂肪酸而形成的氢过氧脂肪酸衍生物优选选自二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇、以及它们的混合物，优选二羟基脂肪酸。
多结构域酶
在本发明的另一个实施方案中，至少一种氢过氧脂肪酸生成酶和至少一种氢过氧脂肪酸转化酶在多肽链中连接在一起。在另一个实施方案中，所述清洁组合物包含含有多肽结构域的一条或多条多结构域多肽链，所述多肽结构域包括：a)氢过氧脂肪酸生成结构域，和b)氢过氧脂肪酸转化结构域，优选地所述氢过氧脂肪酸生成结构域是双加氧酶(DOX)结构域，并且优选地所述氢过氧脂肪酸转化结构域选自丙二烯氧化物合酶(AOS)结构域、环氧醇合酶(EAS)结构域、氢过氧化物裂解酶(HPL)结构域和氢过氧化物异构酶(HPI)结构域。在反应的第一步中，这些酶的DOX结构域将不饱和脂肪酸转化为氢过氧脂肪酸，随后往往进行由HPI、AOS或EAS结构域催化的另外的转化。据信当两种酶连接在一起时，转化速率和对氢过氧脂肪酸的期望衍生物的选择性可得到增强。
包括a)氢过氧脂肪酸生成结构域和b)氢过氧脂肪酸转化结构域的多结构域酶的若干实例存在于自然界中。本发明包括的这些多结构域酶的实例为二醇合酶。在本申请的上下文中，“二醇合酶”是能够将至少一种不饱和脂肪酸转化为包含至少二羟基脂肪酸的氧脂素混合物的酶。本领域已经报道了至少两种不同类的二醇合酶。I类真菌二醇合酶，也称为psi因子生成加氧酶(Ppo)，其包含N-末端双加氧酶(DOX)结构域和C-末端细胞色素P450/氢过氧化物异构酶(HPI)结构域；而II类细菌二醇合酶由N-末端丙二烯氧化物合酶(AOS)结构域和C-末端双加氧酶(DOX)结构域组成。在反应的第一步中，不饱和脂肪酸(例如亚油酸)通过DOX结构域被转化为氢过氧脂肪酸衍生物，随后往往通过HPI结构域或AOS结构域被异构化为二羟基脂肪酸。
若干个氨基酸残基在I类二醇合酶中是保守的。例如，在DOX结构域中，含有催化性Tyr的YR(W/F)H基序是高度保守的。在HPI结构域中，SRS-4区域基序ANQXQ、EXXG基序和血红素特征基序(G/E)(P/A)HX(C/S)(L/F/G)也经常存在于二醇合酶中。血红素基序的His残基和Cys残基以及SRS-4区的最后一个Asn与异构化步骤和反应期间产生的氧脂素的类型有关。
本发明包含不同组的二醇合酶，包括亚油酸二醇合酶和油酸二醇合酶。尽管油酸二醇合酶通常将油酸/油酸酯识别为优选的底物，亚油酸二醇合酶将亚油酸/亚油酸酯识别为优选的底物，但术语“油酸二醇合酶”和“亚油酸二醇合酶”在本文中可互换使用并且不暗示任何底物特异性，即，各自的酶可作用于两种底物。
基于反应产物，已表征了若干种二醇合酶：5,8-亚油酸二醇合酶(5,8-LDS)、7,8-亚油酸二醇合酶(7,8-LDS)、8,11-亚油酸二醇合酶(8,11-LDS)和9,14-亚油酸二醇合酶(9,14-LDS)。尽管它们经常被称为亚油酸二醇合酶，但是它们可以转化不同于亚油酸酯(例如油酸酯)的底物。
本发明包括的二醇合酶的非限制性实例列于表4中。例如，构巢曲霉PpoA(SEQ IDNO:56)将C16和C18不饱和脂肪酸(包括棕榈油酸、油酸、亚油酸和α-亚麻酸)转化成5,8-二羟基脂肪酸，并将C20不饱和脂肪酸(包括二十碳烯酸、二十碳二烯酸和二十碳三烯酸)转化成7,10-二羟基脂肪酸(Brodhun,F.等人(2009),J.Biol.Chem.284(18):11792-11805，以及Jerneren,F.等人(2010),Biochim.Biophys.Acta,Mol.Cell Biol.Lipids 1801(4):503-507)。在另一个实例中，小麦全蚀病菌7,8-LDS(SEQ ID NO:67)将油酸、亚油酸和α-亚麻酸转化为7,8-二羟基脂肪酸作为主要产物，但当γ-亚麻酸、二十碳三烯酸、花生四烯酸和二十碳五烯酸用作底物时，该酶不显示活性。类似地，苹果炭疽病菌7,8-LDS(SEQ ID NO:66)将棕榈油酸、油酸、亚油酸和α-亚麻酸转化为7,8-二羟基脂肪酸，而且通过仅使用N-末端双加氧酶结构域将二十碳烯酸、二十碳二烯酸、二高-γ-亚麻酸和花生四烯酸加工成8-氢过氧脂肪酸(Seo,M.-J.等人(2016),Appl.Microbiol.Biotechnol.100(7):3087-3099)。此外，产黄青霉8,11-LDS(SEQ ID NO:70)将亚油酸和α-亚麻酸转化为8,11-二羟基脂肪酸，而油酸和棕榈油酸转化为8-羟基脂肪酸。有意思的是，苹果炭疽病菌7,8-LDS的Q898E或Q898L变体也将亚油酸转化为8,11-二羟基脂肪酸(Shin,K.-C.等人(2016),J.Lipid Res.57(2):207-218)。念珠藻属PCC 7120 9,14-LDS(SEQ ID NO:2)将亚油酸与9,10-二羟基、8-11-二羟基和9-羟基脂肪酸转化为主产物9,14-二羟基脂肪酸；将α-亚麻酸转化为9,16-二羟基、9,13-二羟基和9-羟基脂肪酸；并且将γ-亚麻酸转化为9,14-二羟基和9-羟基脂肪酸(Lang,I.等人(2008),Biochem.J.410(2):347-357)。
表4：二醇合酶
在自然界中已经观察到其他二羟基化模式，但是酶还没有被表征。例如，巨大芽孢杆菌(Bacillus megaterium)ALA2和棍状杆菌属(Clavibacter sp.)ALA2从亚油酸产生12,13-二羟基脂肪酸。在另一个实例中，红藻龙须菜(Gracilariopsis lemaneiformis)从花生四烯酸产生9,10-二羟基、12,12-二羟基和12-羟基脂肪酸。最后，水节霉(Leptomituslacteus)将亚油酸转化为8,11-二羟基、11,16-二羟基、11,17-二羟基、7-羟基和8-羟基脂肪酸。因此，二醇合酶可用于将不饱和脂肪酸转化为不同的羟基化产物(Kim,K.-R.和D.-K.Oh(2013),Biotechnol.Adv.31(8):1473-1485)。
在另一个实施方案中，二醇合酶选自：亚油酸二醇合酶(EC 1.13.11.44)、5,8-亚油酸二醇合酶(EC 1.13.11.60和EC 5.4.4.5)、7,8-亚油酸二醇合酶(EC 1.13.11.60和EC5.4.4.6)、9,14-亚油酸二醇合酶(EC 1.13.11.B1)、8,11-亚油酸二醇合酶、油酸二醇合酶、以及它们的混合物。
在另一个实施方案中，二醇合酶与选自以下的野生型二醇合酶中的一种或多种具有至少60％，优选至少70％，优选至少80％，优选至少85％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性：构巢曲霉PpoA(SEQ ID NO:56)、烟曲霉PpoA(SEQID NO:61)、土曲霉PpoA(SEQ ID NO:62)、白曲霉PpoA(SEQ ID NO:63)、棒曲霉PpoA(SEQ IDNO:64)、黑曲霉PpoA(SEQ ID NO:65)、苹果炭疽病菌7,8-LDS(SEQ ID NO:66)、小麦全蚀病菌7,8-LDS(SEQ ID NO:67)、稻瘟病菌7,8-LDS(SEQ ID NO:68)、点形念珠藻8,11-LDS(SEQID NO:69)、产黄青霉8,11-LDS(SEQ ID NO:70)、指状青霉8,11-LDS(SEQ ID NO:71)、念珠藻属PCC 7120 9,14-LDS(SEQ ID NO:2)、推定的深海单细胞蓝细菌9,14-LDS(SEQ ID NO:72)和推定的念珠藻属NIES-4103 9,14-LDS(SEQ ID NO:73)。
优选地，二醇合酶是5,8-亚油酸二醇合酶(EC 1.13.11.60和EC 5.4.4.5)。优选地，5,8-亚油酸二醇合酶(EC 1.13.11.60和EC 5.4.4.5)与选自以下的野生型二醇合酶中的一种或多种具有至少60％，优选至少70％，优选至少80％，优选至少85％，优选至少90％，优选至少95％，优选至少98％或优选甚至100％的同一性：构巢曲霉PpoA(SEQ ID NO:56)、烟曲霉PpoA(SEQ ID NO:61)、土曲霉PpoA(SEQ ID NO:62)、白曲霉PpoA(SEQ ID NO:63)、棒曲霉PpoA(SEQ ID NO:64)和黑曲霉PpoA(SEQ ID NO:65)、以及它们的混合物，更优选构巢曲霉PpoA(SEQ ID NO:56)。
在包含a)氢过氧脂肪酸生成结构域和b)氢过氧脂肪酸转化结构域的多结构域酶的另一个实例中，含有双加氧酶(DOX)结构域和丙二烯氧化物合酶(AOS)结构域的酶产生一系列不同的不饱和脂肪酸的含氧衍生物并且包括在本发明中。这些DOX-AOS酶的非限制性实例列于表5中。在另一个实例中，酶可以含有双加氧酶(DOX)结构域和环氧醇合酶(EAS)结构域并且也包括在本发明中。。这些DOX-EAS酶的非限制性实例列于表5中。
表5：其他多结构域酶
优选地，基于组合物中的活性蛋白质，氢过氧脂肪酸生成酶以组合物重量的0.0001重量％至1重量％的量存在。更优选地，基于组合物中的活性蛋白质，氢过氧脂肪酸生成酶以组合物重量的0.001重量％至0.2重量％的量存在。
在本发明的一个实施方案中，通过氢过氧脂肪酸生成酶转化的脂肪酸选自：单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、饱和脂肪酸、以及它们的混合物；优选肉豆蔻油酸、肉豆蔻酸、十五烷酸、棕榈油酸、棕榈酸、十六碳烯酸(sapienic acid)、十七烷酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、硬脂酸、鳕油酸、花生酸、山萮酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物，优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物，更优选油酸。
在另一个实施方案中，由脂肪酸与氢过氧脂肪酸生成酶的转化反应所形成的所得氢过氧脂肪酸选自2-氢过氧脂肪酸、8R-氢过氧脂肪酸、8S-氢过氧脂肪酸、9R-氢过氧脂肪酸、9S-氢过氧脂肪酸、10R-氢过氧脂肪酸、11R-氢过氧脂肪酸、11S-氢过氧脂肪酸、12R-氢过氧脂肪酸、12S-氢过氧脂肪酸、13R-氢过氧脂肪酸、13S-氢过氧脂肪酸、14R-氢过氧脂肪酸、14S-氢过氧脂肪酸、15S-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；优选2R-氢过氧脂肪酸、不饱和5S-氢过氧脂肪酸、不饱和8R-氢过氧脂肪酸、不饱和9R-氢过氧脂肪酸、不饱和11R-氢过氧脂肪酸、不饱和12R-氢过氧脂肪酸、不饱和12S-氢过氧脂肪酸、不饱和13S-氢过氧脂肪酸、不饱和15S-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；更优选2R-氢过氧脂肪酸。所得氢过氧脂肪酸可自发或酶促转化为单羟基脂肪酸、二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇或其他衍生物。单羟基脂肪酸的非限制性实例为2-羟基脂肪酸、不饱和5-羟基脂肪酸、不饱和8-羟基脂肪酸、不饱和9-羟基脂肪酸、不饱和11-羟基脂肪酸、不饱和12-羟基脂肪酸、不饱和13-羟基脂肪酸、不饱和15-羟基脂肪酸、它们的衍生物、以及它们的混合物。二羟基脂肪酸的非限制性实例为5,8-二羟基脂肪酸、7,8-二羟基脂肪酸、7,10-二羟基脂肪酸、8,11-二羟基脂肪酸、9,14-二羟基脂肪酸、以及它们的混合物。醛的非限制性实例为1-链烷醛、链烯-1-醛、链二烯-1-醛、链三烯-1-醛、链四烯-1-醛、以及它们的混合物。
羟基脂肪酸生成酶
本发明的污垢转化酶可以是羟基脂肪酸生成酶，优选选自过加氧酶、脂肪酸水合酶、以及它们的混合物。
过加氧酶
脂肪酸可以在过氧化氢(H2O2)存在下通过过加氧酶而氧化，从而生成羟基脂肪酸。在本发明的另一个实施方案中，所述清洁组合物包含一种或多种酶，所述一种或多种酶选自：非特异性过加氧酶(EC 1.11.2.1)、植物种子过加氧酶(EC 1.11.2.3)、脂肪酸过加氧酶(EC 1.11.2.4)、以及它们的混合物。过加氧酶，也称为氢过氧化物氧化还原酶，可以使用过氧化氢作为共底物催化脂肪酸转化为羟基化衍生物。本发明包括的过加氧酶的非限制性实例列于表6中。例如，少动鞘氨醇单胞菌(Sphingomonas paucimobilis)过加氧酶催化脂肪酸在α-位的羟基化，而枯草芽孢杆菌过加氧酶催化α-位(40％)和β-位(60％)处的羟基化。
表6：过加氧酶
在本发明的一个实施方案中，通过过加氧酶转化的脂肪酸选自单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、饱和脂肪酸、以及它们的混合物；优选肉豆蔻油酸、肉豆蔻酸、十五烷酸、棕榈油酸、棕榈酸、十六碳烯酸、十七烷酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、硬脂酸、鳕油酸、花生酸、山萮酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物，优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物，更优选油酸。
在另一个实施方案中，由脂肪酸与过加氧酶的转化反应所形成的所得羟基脂肪酸选自2-羟基脂肪酸、3-羟基脂肪酸、4-羟基脂肪酸、5-羟基脂肪酸、6-羟基脂肪酸、以及它们的混合物；优选2-羟基脂肪酸、3-羟基脂肪酸、以及它们的混合物。
脂肪酸水合酶
不饱和脂肪酸可以在水(H2O)存在下通过脂肪酸水合酶而水合，从而生成羟基脂肪酸。在另一个实施方案中，所述清洁组合物包含一种或多种脂肪酸水合酶(EC4.2.1.53)。脂肪酸水合酶催化水对不饱和脂肪酸双键的区域特异性且不可逆加成，从而生成羟基脂肪酸。不饱和脂肪酸的水合在细菌中是常见的。例如，还原亚硝酸盐寡养单胞菌(Stenotrophomonas nitritireducens)在C-10位处对亚油酸进行水合，而鹑鸡肠球菌(Enterococcus gallinarum)、黄杆菌属(Flavobacterium sp.)、乳杆菌属(Lactobacillussp.)、乳酸片球菌(Pediococcus acidilactici)和反刍动物月形单胞菌(Selenomonasruminantium)对亚油酸进行水合。油酸水合酶可以将顺-9和顺-12不饱和脂肪酸转化为10-或13-羟基脂肪酸或10,13-二羟基脂肪酸。油酸水合酶的非限制性实例列于表7中。
表7：脂肪酸水合酶
在本发明的一个实施方案中，通过水合酶转化的脂肪酸选自单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、以及它们的混合物；优选肉豆蔻油酸、棕榈油酸、十六碳烯酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、鳕油酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物；优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物；更优选油酸。
在另一个实施方案中，由脂肪酸与水合酶的转化反应所形成的所得羟基脂肪酸选自10-羟基脂肪酸、13-羟基脂肪酸、10,13-二羟基脂肪酸、以及它们的混合物。
附加的考虑
本发明还包括酶的变体。如本文所用，酶的变体包括当相应蛋白质的野生型蛋白质被一个或多个氨基酸(例如1、2、5或10个氨基酸)修饰或在所述一个或多个氨基酸处被修饰时产生的序列。本发明还包括来源于野生型酶的截短形式的变体，例如具有截短的N-末端或截短的C-末端的蛋白质。一些酶可以包括在被细胞分泌时可能被除去的N-末端信号肽。本发明包括没有N-末端信号肽的变体。生物信息学工具，例如SignalP ver 4.1(Petersen TN.,Brunak S.,von Heijne G.和Nielsen H.(2011),Nature Methods,8:785-786)可用于预测这类信号肽的存在和长度。本发明还包括通过向野生型蛋白添加额外的氨基酸序列，例如N-末端标签、C-末端标签或在蛋白序列中间的插入物而衍生的变体。标签的非限制性实例为麦芽糖结合蛋白(MBP)标签、谷胱甘肽S-转移酶(GST)标签、硫氧还蛋白(Trx)标签、His-标签和本领域的技术人员已知的任何其他标签。标签可用于在发酵期间改善溶解度和表达水平或作为酶纯化的柄。
重要的是酶的变体保留并优选地改善野生型蛋白质催化不饱和脂肪酸转化的能力。当然，可以容许变体的给定性质的一些性能下降，但是变体应当保持并且优选地改善它们所针对的相关应用的合适性质。筛选野生型之一的变体可用于鉴定它们是否保留并优选改善适当的性质。
变体可以具有“保守”取代。保守取代的合适实例包括酶中的一个保守取代，例如以下序列中的保守取代：SEQ ID NO:1、SEQ ID NO:2、SEQ ID NO:3、SEQ ID NO:4、SEQ IDNO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ IDNO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ IDNO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ ID NO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ IDNO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ ID NO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ IDNO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ ID NO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ IDNO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ ID NO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ IDNO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ ID NO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ IDNO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ IDNO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ IDNO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ IDNO:77、SEQ ID NO:78、SEQ ID NO:79或SEQ ID NO:80。其他合适的实例包括蛋白质中10个或更少(例如五个或更少)的保守取代。因此，本发明的酶可以包括1、2、3、4、5、6、7、8、9、10或更多个保守取代。含有一个或多个保守取代的酶可以如下方式产生：通过使用例如标准方法，例如定点诱变或PCR，对编码该酶的核苷酸序列进行操纵。
可以取代酶中原始氨基酸并被认为是保守取代的氨基酸的实例包括：Ser取代Ala；Lys取代Arg；Gln或His取代Asn；Glu取代Asp；Asn取代Gln；Asp取代Glu；Pro取代Gly；Asn或Gln取代His；Leu或Val取代Ile；Ile或Val取代Leu；Arg或Gln取代Lys；Leu或Ile取代Met；Met、Leu或Tyr取代Phe；Thr取代Ser；Ser取代Thr；Tyr取代Trp；Trp或Phe取代Tyr；以及Ile或Leu取代Val。
变体包括“经修饰的酶”或“突变酶”，其包括具有至少一个氨基酸取代、插入和/或缺失的蛋白质。经修饰的酶可具有1、2、3、4、5、6、7、8、9或10或更多个氨基酸修饰(选自取代、插入、缺失及其组合)。
酶可以通过多种化学技术修饰，以产生具有与未经修饰的酶基本上相同或优选改善的活性，并任选具有其他所需性质的衍生物。例如，蛋白质的羧酸基团，无论是羧基末端还是侧链，可以以药学上可接受的阳离子盐的形式提供或酯化，例如以形成C1-C6烷基酯，或转化为酰胺，例如式CONR1R2的酰胺，其中R1和R2各自独立地为H或C1-C6烷基，或组合成杂环，例如5-或6-元环。酶的氨基基团，无论是氨基末端还是侧链，可以是药学上可接受的酸加成盐形式，例如HCI、HBr、乙酸、苯甲酸、甲苯磺酸、马来酸、酒石酸和其他有机盐，或者可以被修饰成C1-C20烷基或二烷基氨基或进一步转化为酰胺。蛋白质侧链的羟基基团可以使用公知的技术转化为烷氧基或酯基，例如C1-C20烷氧基或C1-C20烷基酯。蛋白质侧链的苯基环和酚环可以被一个或多个卤素原子(诸如F、Cl、Br或I)，或被C1-C20烷基、C1-C20烷氧基、羧酸及其酯，或此类羧酸的酰胺取代。蛋白质侧链的亚甲基基团可以延伸到同源C2-C4亚烷基。硫醇可以用许多公知的保护基中的任何一种(例如乙酰胺基团)保护。本领域的技术人员还将认识到用于将环状结构引入本公开的蛋白质中以选择并提供对结构的构象限制从而导致增强的稳定性的方法。
如本文所提及，关于本发明的同一性或同源性百分比是可以用GAP程序计算的那些，该GAP程序可从GCG(Genetics Computer Group Inc.,Madison,Wl,USA)获得。另选地，可以执行手动比对。
对于酶序列比较，可以使用以下设置：比对算法：Needleman和Wunsch,J.Mol.Biol.1970,48:443-453。使用Blosum62矩阵作为氨基酸相似性的比较矩阵(Henikoff S.和Henikoff J.G.,P.N.A.S.USA 1992,89:10915-10919)。使用以下空位评分参数：空位罚分：12，空位长度罚分：2，末端空位无罚分。
通常将给定序列与下列的全长序列进行比较，以得到分数：SEQ ID NO:1、SEQ IDNO:2、SEQ ID NO:3、SEQ ID NO:4、SEQ ID NO:5、SEQ ID NO:6、SEQ ID NO:7、SEQ ID NO:8、SEQ ID NO:9、SEQ ID NO:10、SEQ ID NO:11、SEQ ID NO:12、SEQ ID NO:13、SEQ ID NO:14、SEQ ID NO:15、SEQ ID NO:16、SEQ ID NO:17、SEQ ID NO:18、SEQ ID NO:19、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ IDNO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ IDNO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ IDNO:38、SEQ ID NO:39、SEQ ID NO:40、SEQ ID NO:41、SEQ ID NO:42、SEQ ID NO:43、SEQ IDNO:44、SEQ ID NO:45、SEQ ID NO:46、SEQ ID NO:47、SEQ ID NO:48、SEQ ID NO:49、SEQ IDNO:50、SEQ ID NO:51、SEQ ID NO:52、SEQ ID NO:53、SEQ ID NO:54、SEQ ID NO:55、SEQ IDNO:56、SEQ ID NO:57、SEQ ID NO:58、SEQ ID NO:59、SEQ ID NO:60、SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ IDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79或SEQID NO:80。
酶可通过添加剂颗粒(例如酶颗粒)或以包封物的形式掺入清洁组合物中，或者可以液体制剂的形式加入。优选地，酶通过包封物掺入清洁组合物中。包封酶促进了酶在组合物中的稳定性，并有助于抵消组合物中存在的任何不利化合物例如漂白剂、蛋白酶、表面活性剂、螯合剂等的影响。
存在于添加剂颗粒中的氢过氧脂肪酸生成酶可以是添加剂颗粒中唯一的酶，或者可以与一种或多种附加的辅酶组合存在于添加剂颗粒中。
必要时，该组合物包含，提供获取途径或原位形成对于酶的有效功能而言所必需的任何附加底物。例如，提供分子氧作为脂氧合酶、α-双加氧酶、二醇合酶和其他含有DOX结构域的酶的附加底物。分子氧可以从大气中获得，或者从可被转化以原位生成氧的前体获得。在许多应用中，来自大气的氧可以足够的量存在。类似地，过氧化氢作为过加氧酶的附加底物提供，并且可添加到清洁组合物中或在清洁过程中原位生成，例如通过醇氧化酶对其对应底物的作用。此外，需要将水作为脂肪酸水合酶的附加底物，并且水可以是清洁组合物的一部分或在清洁过程中加入。
表面活性剂
本发明的消费产品组合物还包含表面活性剂，所述表面活性剂的含量按所述组合物的重量计优选为约0.01％至约60％，优选约5％至约50％，更优选约8％至约40％。
在一些方面，所述消费产品组合物包含相对低含量的表面活性剂，因为污垢转化酶可将直接位于被清洁表面上的含脂肪酸的污垢转化为活性脂肪酸衍生物物质，以便有助于从表面清洁污垢。因此，此类活性脂肪酸衍生物物质可减少在消费产品组合物中掺入较大量的表面活性剂的需求。此外，较低含量的表面活性剂可有利于本文所用的污垢转化酶的稳定性和活性。因此，所述消费产品组合物优选包含按所述组合物的重量计约0.01％至约2％，优选约0.01％至约1.5％，优选约0.01％至约1％，优选约0.01％至约0.5％，优选约0.01％至约0.2％，优选约0.01％至约0.1％的表面活性剂。
合适的表面活性剂选自阴离子表面活性剂、非离子表面活性剂、两性离子表面活性剂、两性表面活性剂、阳离子表面活性剂、以及它们的混合物；如果该组合物是衣物洗涤剂或洗发剂，则通常采用阴离子和非离子表面活性剂。如果组合物是织物柔软剂或毛发调理剂，则通常使用阳离子表面活性剂。
适用于所述组合物中的阴离子表面活性剂包括烷基硫酸盐和烷基醚硫酸盐。其它合适的阴离子表面活性剂为有机硫酸反应产物的水溶性盐。其它合适的阴离子表面活性剂为用羟乙磺酸酯化并且用氢氧化钠中和的脂肪酸的反应产物。其它类似的阴离子表面活性剂描述于美国专利2,486,921；2,486,922；和2,396,278中。
用于所述组合物中的示例性阴离子表面活性剂包括月桂基硫酸铵、月桂基聚氧乙烯醚硫酸铵、月桂基硫酸三乙基胺、月桂基聚氧乙烯醚硫酸三乙基胺、月桂基硫酸三乙醇胺、月桂基聚氧乙烯醚硫酸三乙醇胺、月桂基硫酸单乙醇胺、月桂基聚氧乙烯醚硫酸单乙醇胺、月桂基硫酸二乙醇胺、月桂基聚氧乙烯醚硫酸二乙醇胺、月桂酸单甘油酯硫酸钠、月桂基硫酸钠、月桂基聚氧乙烯醚硫酸钠、月桂基硫酸钾、月桂基聚氧乙烯醚硫酸钾、月桂基肌氨酸钠、月桂酰肌氨酸钠、月桂基肌氨酸、椰油酰肌氨酸、椰油酰硫酸铵、月桂酰硫酸铵、椰油酰硫酸钠、月桂酰硫酸钠、椰油酰硫酸钾、月桂基硫酸钾、月桂基硫酸三乙醇胺、月桂基硫酸三乙醇胺、椰油酰硫酸单乙醇胺、月桂基硫酸单乙醇胺、十三烷基苯磺酸钠、十二烷基苯磺酸钠、椰油酰羟乙磺酸钠以及它们的组合。在另一个实施方案中，阴离子表面活性剂为月桂基硫酸钠或月桂基聚氧乙烯醚硫酸钠。
所述组合物可包含非离子表面活性剂。组合物可包含按所述组合物的重量计至多0.01％至约30％，或约0.01％至约20％，还或约0.1％至约10％的非离子表面活性剂。在一些示例中，非离子表面活性剂可包括乙氧基化非离子表面活性剂。适用于本文的为式R(OC2H4)n OH的乙氧基化醇和乙氧基化烷基酚，其中R选自含有约8至约20个碳原子的脂族烃基团、和其中烷基基团含有约8至约12个碳原子的烷基苯基基团，并且n的平均值为约5至约15。
合适的非离子表面活性剂为式R1(OC2H4)nOH的那些，其中R1为C10-C16烷基基团或C8-C12烷基苯基基团，并且n为3至约80。在一个方面，尤其可用的材料为C9-C15醇与每摩尔醇约5至约20摩尔环氧乙烷的缩合产物。
合适的阳离子表面活性剂包括可递送织物护理有益效果的那些，其非限制性实例包括：脂肪胺；季铵表面活性剂；和咪唑啉季铵盐材料。
阳离子表面活性剂的非限制性示例为N,N-双(硬脂酰-氧乙基)N,N-二甲基氯化铵、N,N-双(牛油酰-氧乙基)N,N-二甲基氯化铵、N,N-双(硬脂酰-氧乙基)N-(2-羟乙基)N-甲基甲硫酸铵；1,2二(硬脂酰氧基)3三甲基丙基氯化铵；二亚烷基二甲基铵盐诸如二低芥酸菜籽基二甲基氯化铵、二(硬)牛油二甲基氯化铵、二低芥酸菜籽基二甲基甲酯硫酸铵；1-甲基-1-硬脂酰氨基乙基-2-硬脂酰基咪唑啉甲基硫酸盐；1-牛油基酰氨基乙基-2-牛油基咪唑啉；N,N″-二烷基二亚乙基三胺；用脂肪酸酯化的N-(2-羟乙基)-1,2-乙二胺或N-(2-羟基异丙基)-1,2-乙二胺与乙醇酸的反应产物，其中脂肪酸为(氢化)牛油脂肪酸、棕榈脂肪酸、氢化棕榈脂肪酸、油酸、油菜籽脂肪酸、氢化油菜籽脂肪酸；聚甘油酯(PGE)、油性糖衍生物和蜡乳液以及上述物质的混合物。
阳离子表面活性剂可用作消费产品组合物中的调理剂，诸如在织物软化组合物或毛发调理组合物中用作调理剂。
适用于毛发护理组合物中的两性去污表面活性剂包括被广泛地描述为脂族仲胺和叔胺的衍生物的那些表面活性剂，其中脂族基团可为直链或支链，并且其中脂族取代基中的一个包含约8至约18个碳原子，并且一个脂族取代基包含阴离子基团诸如羧基、磺酸根、硫酸根、磷酸根或膦酸根。用于本发明的毛发护理组合物中的示例性两性去污表面活性剂包括椰油酰两性基乙酸盐、椰油酰两性基二乙酸盐、月桂酰两性基乙酸盐、月桂酰两性基二乙酸盐、以及它们的混合物。
适用于毛发护理组合物中的两性离子去污表面活性剂包括被广泛地描述为脂族季铵、鏻鎓和锍鎓化合物的衍生物的那些表面活性剂，其中脂族基团可为直链或支链，并且其中脂族取代基中的一个包含约8至约18个碳原子，并且一个脂族取代基包含阴离子基团诸如羧基、磺酸根、硫酸根、磷酸根或膦酸根。在另一个实施方案中，选择两性离子化物，诸如甜菜碱。
合适的两性离子表面活性剂或两性表面活性剂的非限制性示例描述于美国专利5,104,646和5,106,609中，这些文献全文以引用方式并入本文。其他适用于组合物中的阴离子表面活性剂、两性离子表面活性剂、两性表面活性剂或任选的附加表面活性剂的非限制性示例描述于McCutcheon的《Emulsifiers and Detergents》(1989年鉴，由M.C.Publishing Co.)出版和美国专利3,929,678、2,658,072；2,438,091；2,528,378中，这些文献全文以引用方式并入本文。
在一个方面，可优选的是消费产品组合物不含硫酸盐表面活性剂。在此类方面，表面活性剂优选为糖基表面活性剂或氨基酸基表面活性剂，例如选自癸基葡糖苷、羟乙基磺酸盐、谷氨酸盐以及它们的混合物的表面活性剂。
在一个方面，所述消费产品组合物可包含阴离子表面活性剂和辅助表面活性剂，所述辅助表面活性剂选自两性表面活性剂、两性离子表面活性剂以及它们的混合物。优选地，两性表面活性剂是氧化胺表面活性剂，并且两性离子表面活性剂是甜菜碱表面活性剂。
优选地，阴离子表面活性剂与一种或多种辅助表面活性剂的重量比小于9:1，更优选5:1至1:1，更优选4:1至2:1。优选地，主要辅助表面活性剂体系是两性表面活性剂。优选地，主要辅助表面活性剂体系是氧化胺表面活性剂，并且其中所述组合物包含重量比小于9:1，更优选5:1至1:1，更优选4:1至2:1，优选3:1至2.5:1的阴离子表面活性剂和氧化胺表面活性剂。优选地，对于本发明的组合物，其中表面活性剂体系包含一种或多种阴离子表面活性剂和一种或多种辅助表面活性剂，其中阴离子表面活性剂为烷基硫酸盐和烷基烷氧基硫酸盐的混合物，辅助表面活性剂为烷基二甲基氧化胺，并且其中阴离子表面活性剂与辅助表面活性剂的重量比为4:1至2:1。
优选的氧化胺是烷基二甲基氧化胺或烷基酰氨基丙基二甲基氧化胺，更优选地烷基二甲基氧化胺，并且尤其是椰油二甲基氧化胺。氧化胺可具有直链或支链的烷基部分。
所述组合物还可包含C10AO，尤其是正癸基二甲胺，并且优选包含按总氧化胺的重量计小于5％，优选小于3％的C8氧化胺，例如C8二甲基氧化胺。
辅助表面活性剂可为两性离子表面活性剂。两性离子表面活性剂的合适实例包括甜菜碱，优选烷基甜菜碱、烷基酰胺基甜菜碱、以及它们的混合物。最优选椰油酰胺丙基甜菜碱。
所述消费产品组合物还可包含辅助表面活性剂，所述辅助表面活性剂包括非离子表面活性剂。合适的非离子表面活性剂包括脂族醇与1摩尔至25摩尔环氧乙烷的缩合产物。脂族醇的烷基链可为直链或支链、伯烷基或仲烷基，并通常含有8至22个碳原子。尤其优选的是具有具有10至18个碳原子，优选地10至15个碳原子的烷基的醇与每摩尔醇2摩尔至18摩尔，优选地2摩尔至15摩尔，更优选5摩尔至12摩尔的环氧乙烷的缩合产物。高度优选的非离子表面活性剂是格尔伯特醇与每摩尔醇2摩尔至18摩尔，优选地2摩尔至15摩尔，更优选地5摩尔至12摩尔的环氧乙烷的缩合产物。优选地，非离子表面活性剂是烷基乙氧基化表面活性剂，优选在其烷基链中包含9至15个碳原子并且每摩尔醇包含5至12个环氧乙烷单元。用于本文的其他合适的非离子表面活性剂包括脂肪醇聚乙二醇醚、烷基多聚葡糖苷和脂肪酸葡糖酰胺，优选烷基多聚葡糖苷。优选地，烷基多聚葡糖苷表面活性剂是C8-C16烷基多聚葡糖苷表面活性剂，优选C8-C14烷基多聚葡糖苷表面活性剂，优选具有在0.1至3之间，更优选在0.5至2.5之间，甚至更优选在1至2之间的平均聚合度。最优选地，烷基多聚葡糖苷表面活性剂具有在10至16，优选在10至14之间，最优选在12至14之间的平均烷基碳链长度，其中平均聚合度为在0.5至2.5之间，优选在1至2之间，最优选在1.2至1.6之间。C8-C16烷基多聚葡糖苷可从几个供应商处商购获得(例如，购自Seppic公司的
消费产品助剂成分
本文的消费产品组合物可任选地包含许多其他消费产品助剂成分，诸如酶稳定剂、辅酶、盐、水溶助长剂、螯合剂、助洗剂、分散剂、染料转移抑制剂、漂白剂、稳定剂/增稠剂、香料、调理剂、调色剂、结构剂、溶剂、含水载体、以及它们的混合物。消费产品助剂成分还包括洗擦颗粒、恶臭控制剂、颜料、染料、遮光剂、pH调节剂和缓冲物(例如，羧酸如柠檬酸、HCl、NaOH、KOH、链烷醇胺、磷酸和磺酸，碳酸盐如碳酸钠、碳酸氢盐、倍半碳酸盐、硼酸盐、硅酸盐、磷酸盐、咪唑等)。
酶稳定剂
优选地，本发明的组合物还包含酶稳定剂，所述酶稳定剂选自化学稳定剂和物理稳定剂，优选地，所述物理稳定剂包括包封所述酶。合适的酶稳定剂可选自由下列构成的组：(a)优选选自由下列构成的组的单价、二价和/或三价阳离子：碱土金属、碱金属、铝、铁、铜和锌的无机或有机盐，优选碱金属和碱土金属，优选碱金属和碱土金属盐与卤化物、硫酸盐、亚硫酸盐、碳酸盐、碳酸氢盐、硝酸盐、亚硝酸盐、磷酸盐、甲酸盐、乙酸盐、丙酸盐、柠檬酸盐、马来酸盐、酒石酸盐、琥珀酸盐、草酸盐、乳酸盐、以及它们的混合物。在优选的实施方案中，盐选自由下列构成的组：氯化钠、氯化钙、氯化钾、硫酸钠、硫酸钾、乙酸钠、乙酸钾、甲酸钠、甲酸钾、乳酸钙、硝酸钙以及它们的混合物。最优选的是选自由下列构成的组的盐：氯化钙、氯化钾、硫酸钾、乙酸钠、乙酸钾、甲酸钠、甲酸钾、乳酸钙、硝酸钙以及它们的混合物的盐，特别是选自由下列构成的组的钾盐：氯化钾、硫酸钾、乙酸钾、甲酸钾、丙酸钾、乳酸钾以及它们的混合物。最优选的是乙酸钾和氯化钾。优选的钙盐是甲酸钙、乳酸钙和硝酸钙，包括硝酸钙四水合物。可优选甲酸钙和甲酸钠盐。这些阳离子以按组合物的总重量计至少0.01重量％，优选至少0.03重量％，更优选至少0.05重量％，最优选至少0.25重量％至最多2重量％或甚至最多1重量％的量存在。这些盐被配制为相对于组合物总重量的0.1重量％至5重量％，优选0.2重量％至4重量％，更优选0.3重量％至3重量％，最优选0.5重量％至2重量％。其它酶稳定剂可选自由下列构成的组：(b)选自由低聚糖、多糖以及它们的混合物构成的组的碳水化合物，如WO201219844中所述的单糖甘油酸酯；(c)高效可逆蛋白酶抑制剂，高效可逆蛋白酶抑制剂选自由下列构成的组：苯基硼酸以及它们的衍生物，优选4-甲酰基苯基硼酸；(d)醇类，如1,2-丙烷二醇、丙二醇；(e)肽醛稳定剂，如三肽醛，如Cbz-Gly-Ala-Tyr-H，或二取代的丙氨酰胺；(f)羧酸，如WO2012/19849中所述的苯基烷基二羧酸或如WO2012/19848中所述的在苄基的至少两个碳原子上包含羧基的多取代苄基羧酸，邻苯二甲酰谷氨酰胺酸，邻苯二甲酰基天冬酰胺酸，氨基邻苯二甲酸和/或低聚氨基-联苯-低聚羧酸；以及(g)它们的混合物。
辅酶
本发明优选的组合物包含选自淀粉酶、脂肪酶、蛋白酶、纤维素酶、脂氧合酶、二醇合酶以及它们的混合物的一种或多种附加的辅酶。本发明的甚至更优选的组合物包含选自脂肪酶、蛋白酶、纤维素酶、淀粉酶以及它们的任意组合的一种或多种辅酶。本发明的最优选的组合物包含选自脂肪酶、蛋白酶、淀粉酶以及它们的任意组合的一种或多种辅酶。
特别优选的是，本发明的组合物另外包含蛋白酶辅酶。由于油酸和其他泡沫抑制不饱和脂肪酸存在于体垢甚至人体皮肤中，因为蛋白酶用作皮肤护理剂或分解蛋白质污垢，因此释放的脂肪酸被分解，防止泡沫抑制。
特别优选的是，本发明的组合物另外包含淀粉酶辅酶。由于油性污垢通常被捕获在淀粉质污垢中，所以淀粉酶和不饱和脂肪酸转化酶一起协同作用：通过用淀粉酶分解淀粉质污垢来释放脂肪酸污垢，因此，不饱和脂肪酸转化酶特别有效地确保不会对洗涤液体中的泡沫产生负面影响。
特别优选的是，本发明的组合物另外包含脂肪酶辅酶。脂肪酶将脂肪酯污垢分解成脂肪酸，然后氢过氧脂肪酸生成酶、过加氧酶和脂肪酸水合酶作用于脂肪酸，生成泡沫或清洁促进剂。
在本发明的另一个实施方案中，消费产品组合物包含一种或多种辅酶，所述一种或多种辅酶选自：非特异性单加氧酶(EC 1.14.14.1)、烷烃1-单加氧酶(EC 1.14.15.3)、油酸12-羟化酶(EC 1.14.18.4)、亚油酸异构酶(EC 5.2.1.5)、亚油酸(10E,12Z)-异构酶(EC5.3.3.B2)、脂肪酸脱羧酶(OleT样)、铁依赖性脱羧酶(UndA样)、其他CYP450单加氧酶、以及它们的混合物。
当存在时，辅酶通常以按组合物的重量计(基于活性蛋白质)约0.0001％至约1％，优选约0.0005％至约0.5％，更优选约0.005％至约0.1％的量存在。
盐
本发明的组合物可任选地包含按总组合物的重量计0.01％至3％，优选0.05％至2％，更优选0.2％至1.5％，或最优选0.5％至1％的盐，优选一价、二价无机盐或它们的混合物，优选氯化钠。最优选地，所述组合物可选地或进一步包含按组合物的重量计0.01％至2％，优选0.1％至1％，更优选0.2％至0.8％的量的多价金属阳离子，优选地所述多价金属阳离子是镁、铝、铜、钙或铁，更优选地是镁，最优选地所述多价盐是氯化镁。据信，多价阳离子的使用有助于在油水和气水界面处形成增强泡沫的蛋白质/蛋白质、表面活性剂/表面活性剂或混合蛋白质/表面活性剂网络。
水溶助长剂
本发明的组合物可以任选地包含按总组合物的重量计1％至10％，或优选0.5％至10％，更优选1％至6％，或最优选0.1％至3％，或它们的组合的水溶助长剂，优选异丙基苯磺酸钠。适用于本文的其他水溶助长剂包括阴离子型水溶助长剂，尤其是二甲苯磺酸钠、二甲苯磺酸钾和二甲苯磺酸铵，甲苯磺酸钠、甲苯磺酸钾和甲苯磺酸铵，异丙基苯磺酸钠、异丙基苯磺酸钾和异丙基苯磺酸铵、以及它们的混合物，如美国专利3,915,903中所公开的。
螯合剂
本文洗涤剂组合物可包含按总组合物的重量计0.1％至20％，优选0.2％至5％，更优选0.2％至3％含量的螯合剂。
合适的螯合剂包括柠檬酸盐；含氮的无磷氨基羧酸盐，诸如EDDS、EDTA和DTPA；氨基膦酸盐，诸如二亚乙基三胺五亚甲基膦酸和乙二胺四亚甲基膦酸；无氮膦酸盐，例如HEDP；以及含氮或氧的无磷无羧酸根的螯合剂，诸如具有某些大环N-配体的一般类别化合物，诸如已知用于漂白催化剂体系中的那些。
用于本发明组合物的其他合适的螯合剂包括羧酸盐螯合剂、氨基羧酸盐螯合剂、氨基膦酸盐螯合剂、以及它们的混合物。优选地，螯合剂选自MGDA(甲基甘氨酸-N,N-二乙酸)、GLDA(谷氨酸-N,N-二乙酸)、以及它们的混合物。
助洗剂
所述组合物还可包含按所述组合物的重量计约0.1％至80％的助洗剂。液体形式的组合物一般包含按所述组合物的重量计约1％至10％的助洗剂组分。颗粒形式的组合物一般包含按所述组合物的重量计约1％至50％的助洗剂组分。洗涤剂助剂是本领域熟知的，并且可包括例如磷酸盐以及各种有机和无机无磷助洗剂。可用于本文的水溶性无磷有机助洗剂包括聚乙酸、羧酸、聚羧酸和多羟基磺酸的各种碱金属盐、铵盐和取代的铵盐。聚乙酸盐助洗剂和聚羧酸盐助洗剂的示例为乙二胺四乙酸、次氮基三乙酸、氧联二琥珀酸、苯六甲酸、多羧酸苯和柠檬酸的钠、钾、锂、铵和取代的铵盐。其它聚羧酸盐助洗剂为氧联二琥珀酸盐和醚羧酸盐助洗剂组合物，该醚羧酸盐助洗剂组合物包含酒石酸一琥珀酸盐和酒石酸二琥珀酸盐的组合。用于液体洗涤剂中的助洗剂包括柠檬酸。合适的无磷无机助洗剂包括硅酸盐、硅铝酸盐、硼酸盐和碳酸盐，诸如碳酸钠和碳酸钾、碳酸氢钠和碳酸氢钾、倍半碳酸钠和倍半碳酸钾、十水四硼酸钠和十水四硼酸钾、以及具有约0.5至约4.0、或约1.0至约2.4的SiO2与碱金属氧化物的重量比的硅酸钠和硅酸钾。也可用的是硅铝酸盐，包括沸石。
分散剂
所述组合物可包含按所述组合物的重量计约0.1％至约10％的分散剂。合适的水溶性有机材料是均聚酸或共聚酸或它们的盐，其中多元羧酸可含有至少两个彼此分离不超过两个碳原子的羧基基团。分散剂也可为聚胺的烷氧基化衍生物和/或季铵化衍生物。
染料转移抑制剂
所述组合物还可包含按所述组合物的重量计约0.0001％，约0.01％，约0.05％，至按所述组合物的重量计约10％，约2％，或甚至约1％的一种或多种染料转移抑制剂，如聚乙烯吡咯烷酮聚合物、聚胺N-氧化物聚合物、N-乙烯基吡咯烷酮和N-乙烯基咪唑的共聚物、聚乙烯噁唑烷酮和聚乙烯咪唑或它们的混合物。
漂白剂体系
适用于本文的漂白剂体系包含一种或多种漂白剂。合适的漂白剂的非限制性示例包括催化金属配合物；活性过氧源；漂白活化剂；漂白增效剂；光漂白剂；漂白酶；自由基引发剂；H2O2；次氯酸盐漂白剂；过氧源，包括过硼酸盐和/或过碳酸盐以及它们的组合。合适的漂白活化剂包括可全水解的酯和可全水解的酰亚胺，诸如四乙酰基乙二胺、辛酰基己内酰胺、苯甲酰氧基苯磺酸酯、壬酰氧基苯磺酸酯、苯甲酰基戊内酰胺、十二烷酰氧基苯磺酸酯。其它漂白剂包括过渡金属与具有指定稳定常数的配体的金属配合物。
稳定剂/增稠剂
所述组合物可包含一种或多种稳定剂和增稠剂。可使用任何合适含量的稳定剂；示例性含量包括按组合物的重量计约0.01％至约20％、约0.1％至约10％、或约0.1％至约3％。适用于本文的稳定剂的非限制性示例包括结晶的含羟基稳定剂、三羟基硬脂酸甘油酯、氢化油、或它们的变型、以及它们的组合。在一些方面，结晶的含羟基稳定剂可为水不溶性蜡状物质，包括脂肪酸、脂肪酸酯或脂肪皂。在其它方面，结晶的含羟基稳定剂可为蓖麻油的衍生物，诸如氢化蓖麻油衍生物，例如蓖麻蜡。含羟基稳定剂公开于美国专利6,855,680和7,294,611中。其它稳定剂包括增稠稳定剂，诸如树胶和其它类似多糖，例如结冷胶、角叉菜胶和其它已知类型的增稠剂和流变添加剂。该类示例性稳定剂包括树胶型聚合物(例如黄原胶)、聚乙烯醇及其衍生物、纤维素及其衍生物(包括纤维素醚和纤维素酯)、和罗望子胶(例如包括木葡聚糖聚合物)、瓜尔胶、刺槐豆胶(在一些方面，包括半乳甘露聚糖聚合物)、以及其它工业树胶和聚合物。
调理剂
消费产品组合物可包含一种或多种调理剂。调理剂包括用于向表面例如毛发或织物提供特定调理或软化有益效果的材料。可用于本发明的消费产品组合物中的调理剂通常包括水不溶性、水分散性、非挥发性、可形成乳化的液体颗粒的液体。合适的调理剂为特征通常为有机硅、有机调理油或它们的组合的那些调理剂，或者在含水表面活性剂基质中以其他方式形成液体、分散颗粒的那些调理剂。
阳离子表面活性剂，例如上文所述的季铵化合物，也可以用作本发明组合物中的调理剂。
一种或多种调理剂以按所述组合物的重量计约0.01％至约10％，约0.1％至约8％，和约0.2％至约4％存在。
有机硅
合适的有机硅包含Si-O部分，并且可选自(a)非官能化硅氧烷聚合物，(b)官能化硅氧烷聚合物，以及它们的组合。有机硅的分子量通常通过参考材料的粘度而示出。在一个方面，有机硅在25℃下可具有约10厘沲至约2,000,000厘沲的粘度。在另一方面，合适的有机硅在25℃下可具有约10厘沲至约800,000厘沲的粘度。
合适的有机硅可为直链、支链或交联的。
在一些示例中，有机硅可包括环状有机硅。环状有机硅可包括式[(CH3)2SiO]n的环甲基硅酮，其中n为可在约3至约7、或约5至约6范围内的整数。
在一些示例中，有机硅可包括官能化硅氧烷聚合物。官能化硅氧烷聚合物可包含一个或多个官能团部分，该官能团部分选自氨基、酰氨基、烷氧基、羟基、聚醚、羧基、氢离子、巯基、硫酸根、磷酸根、和/或季铵部分。这些部分可通过二价亚烷基基团直接连接到硅氧烷主链(即“侧基”)，或可为主链的一部分。合适的官能化硅氧烷聚合物包括选自以下的材料：氨基有机硅、酰氨基有机硅、有机硅聚醚、有机硅-氨基甲酸酯聚合物、季ABn有机硅、氨基ABn有机硅、以及它们的组合。
在一些示例中，官能化硅氧烷聚合物可包括有机硅聚醚，其还称为“聚二甲基硅氧烷共聚多元醇”。一般来讲，有机硅聚醚包含聚二甲基硅氧烷主链与一个或多个聚氧化亚烷基链。聚氧化亚烷基部分可作为侧链或作为末端嵌段掺入聚合物中。在一些示例中，官能化硅氧烷聚合物可包括氨基有机硅。
在一些示例中，有机硅可包括胺ABn有机硅和季ABn有机硅。此类有机硅一般通过二胺与环氧化物反应来制备。
在一些示例中，官能化硅氧烷聚合物可包括有机硅-氨基甲酸酯。这些可以商品名
有机硅材料通常用作消费产品组合物中的调理剂，诸如在织物软化组合物或毛发调理组合物中用作调理剂。
有机调理材料
本发明的组合物的调理剂还可包含单独或与其它调理剂诸如上述有机硅组合的至少一种有机调理材料诸如油或蜡。有机材料可以是非聚合的、低聚的或聚合的。它可为油或蜡的形式，并且可以纯制剂或以预乳化形式添加。有机调理材料的一些非限制性示例包括但不限于：i)烃油；ii)聚烯烃；iii)脂肪酯；iv)氟化调理化合物；v)脂肪醇；vi)烷基葡糖苷和烷基葡糖苷衍生物；vii)季铵化合物；viii)具有至多约2,000,000的分子量的聚乙二醇和聚丙二醇，包括具有CTFA名称PEG-20 200、PEG-400、PEG-600、PEG-1000、PEG-2M、PEG-7M、PEG-14M、PEG-45M的那些、以及它们的混合物。
香料
消费产品助剂成分可包含香料，该香料为除了微胶囊之外另外添加到消费产品组合物中的纯香料。因此，消费产品组合物可包含纯香料和包含香料作为微胶囊的芯材料的微胶囊。纯香料和微胶囊的香料可相同或可不同。
调色剂
组合物可包含调色剂(有时被称为遮光剂、上蓝剂或美白剂)。调色剂通常向被处理表面例如织物提供蓝色或紫色色调。调色剂能够单独使用或组合使用，以产生特定的调色色调和/或对不同的表面类型调色。这可例如通过将红色和蓝绿色染料混合以产生蓝色或紫色色调来提供。调色剂可选自任何已知化学类别的染料，包括但不限于吖啶、蒽醌类(包括多环醌类)、吖嗪、偶氮(例如，单偶氮、双偶氮、三偶氮、四偶氮、多偶氮)、包括预金属化偶氮、苯并二呋喃和苯并二呋喃酮、类胡萝卜素、香豆素、花菁、二氮杂半花菁、二苯甲烷、甲臜、半花菁、靛蓝类、甲烷、萘酰亚胺、萘醌、硝基和亚硝基、噁嗪、酞菁、吡唑类、二苯乙烯、苯乙烯基、三芳基甲烷、三苯甲烷、氧杂蒽以及它们的混合物。
合适的织物调色剂还包括染料、染料-粘土缀合物、以及有机颜料和无机颜料。合适的染料包括小分子染料和聚合物染料。合适的小分子染料包括选自以下的小分子染料：属于酸性、直接、碱性、活性、或水解的活性、溶剂或分散染料的颜色索引(C.I.)分类的染料，例如被分类为蓝、紫、红、绿或黑的染料，并且单独地或组合地提供期望的色调。在另一方面，合适的小分子染料包括选自色素索引(Society of Dyers and Colourists，Bradford，UK)的下列编号的小分子染料：直接紫染料诸如9、35、48、51、66和99，直接蓝染料诸如1、71、80和279，酸性红染料诸如17、73、52、88和150，酸性紫染料诸如15、17、24、43、49和50，酸性蓝染料诸如15、17、25、29、40、45、75、80、83、90和113，酸性黑染料诸如1，碱性紫染料诸如1、3、4、10和35，碱性蓝染料诸如3、16、22、47、66、75和159，分散染料或溶剂染料(US 8,268,016 B2)，或如US 7,208,459 B2中所公开的染料，以及它们的混合物。在另一方面，合适的小分子染料包括选自以下的小分子染料：C.I.编号酸性紫17、酸性蓝80、酸性紫50、直接蓝71、直接紫51、直接蓝1、酸性红88、酸性红150、酸性蓝29、酸性蓝113或它们的混合物。
合适的聚合物染料包括选自以下的聚合物染料：含有共价结合(有时被称为缀合)的色原体的聚合物(染料-聚合物缀合物)(例如具有共聚至该聚合物主链中的色原体的聚合物)以及它们的混合物。聚合物染料包括描述于US 7,686,892B2中的那些。
在一些示例中，适宜的聚合物染料包括选自下列物质的聚合物染料：以商品名
合适的染料粘土缀合物包括选自以下的染料粘土缀合物：至少一种阳离子/碱性染料和绿土粘土、以及它们的混合物。在另一方面，合适的染料粘土缀合物包括选自以下的染料粘土缀合物：一种阳离子/碱性染料，其选自：C.I.碱性黄1至108，C.I.碱性橙1至69，C.I.碱性红1至118，C.I.碱性紫1至51，C.I.碱性蓝1至164，C.I.碱性绿1至14，C.I.碱性棕1至23、CI碱性黑1至11以及粘土，该粘土选自蒙脱石粘土、锂蒙脱石粘土、皂石粘土以及它们的混合物。在另一个方面，合适的染料粘土缀合物包括选自以下的染料粘土缀合物：蒙脱石碱性蓝B7 C.I.42595缀合物、蒙脱石碱性蓝B9 C.I.52015缀合物、蒙脱石碱性紫V3C.I.42555缀合物、蒙脱石碱性绿G1 C.I.42040缀合物、蒙脱石碱性红R1 C.I.45160缀合物、蒙脱石C.I.碱性黑2缀合物、锂蒙脱石碱性蓝B7 C.I.42595缀合物、锂蒙脱石碱性蓝B9C.I.52015缀合物、锂蒙脱石碱性紫V3 C.I.42555缀合物、锂蒙脱石碱性绿G1 C.I.42040缀合物、锂蒙脱石碱性红R1 C.I.45160缀合物、锂蒙脱石C.I.碱性黑2缀合物、皂碱性蓝B7C.I.42595缀合物、皂碱性蓝B9 C.I.52015缀合物、皂碱性紫V3 C.I.42555缀合物、皂碱性绿G1 C.I.42040缀合物、皂碱性红R1 C.I.45160缀合物、皂C.I.碱性黑2缀合物以及它们的混合物。
合适的颜料包括选自以下的颜料：黄烷士酮、靛蒽醌、含有1至4个氯原子的氯化靛蒽醌、皮蒽酮、二氯皮蒽酮、单溴二氯皮蒽酮、二溴二氯皮蒽酮、四溴皮蒽酮、二萘嵌苯-3,4,9,10-四羧酸二酰亚胺，其中酰亚胺基团可为未取代的或者被C1-C3-烷基或苯基或杂环基团取代，并且其中苯基和杂环基团可另外带有不提供水中溶解度的取代基、蒽素嘧啶羧酸酰胺、蒽酮紫、异蒽酮紫、二噁嗪颜料、每个分子可含有至多2个氯原子的铜酞菁、多氯铜酞菁或每个分子含有至多14个溴原子的多溴氯铜酞菁、以及它们的混合物。在另一方面，合适的颜料包括选自以下的颜料：群青蓝(C.I.颜料蓝29)、群青紫(C.I.颜料紫15)、单星蓝以及它们的混合物。
结构剂
可加入以充分悬浮包含有益剂的递送颗粒的有用结构剂材料包括多糖，例如结冷胶、蜡质玉米或马齿种玉米淀粉、辛烯基琥珀酸化淀粉、衍生的淀粉诸如羟乙基化或羟丙基化淀粉、角叉菜胶、瓜耳胶、果胶、黄原胶，以及它们的混合物；改性纤维素，诸如水解纤维素乙酸酯、羟丙基纤维素、甲基纤维素、以及它们的混合物；改性蛋白质，诸如明胶；氢化和未氢化的聚烯烃、以及它们的混合物；无机盐，例如氯化镁、氯化钙、甲酸钙、甲酸镁、氯化铝、高锰酸钾、合成锂皂石粘土、膨润土以及它们的混合物；与无机盐组合的多糖；季铵化聚合物材料，例如聚醚胺、烷基三甲基氯化铵、二酯二牛油基氯化铵；咪唑；具有小于6.0的pKa的非离子聚合物，例如聚乙烯亚胺、聚乙烯亚胺乙氧基化物；聚氨酯。此类材料可得自CPKelco Corp.(San Diego,California,USA)；Degussa AG或Dusseldorf,Germany；BASF AG(Ludwigshafen,Germany)；Rhodia Corp.(Cranbury,New Jersey,USA)；Baker HughesCorp.(Houston,Texas,USA)；Hercules Corp.(Wilmington,Delaware,USA)；Agrium Inc.(Calgary,Alberta,Canada)、ISP(New Jersey,U.S.A.)。
溶剂
本发明的组合物可任选包含有机溶剂。合适的有机溶剂包括C4-14醚和二醚、多元醇、二醇、烷氧基化二醇、C6-C16二醇醚、烷氧基化芳族醇、芳族醇、脂族直链或支链醇、烷氧基化脂族直链或支链醇、烷氧基化C1-C5醇、C8-C14烷基和环烷基烃以及卤代烃、以及它们的混合物。优选地，有机溶剂包括醇、二醇和二醇醚，或者醇和二醇。所述组合物包含按所述总组合物的重量计0％至小于50％，优选0.01％至25％，更优选0.1％至10％，或最优选0.5％至5％的有机溶剂，优选醇，更优选乙醇、聚亚烷基二醇，更优选聚丙二醇、以及它们的混合物。
含水载体
本文的组合物可为可倾倒的液体形式(在环境条件下)。因此，此类组合物通常包含载体，其以约20重量％至约95重量％、或甚至约60重量％至约85重量％的含量存在。所述载体可包括水，或水和有机溶剂的可混溶混合物，并且在一个方面，可包括水与最小或不显著浓度的有机溶剂，除了另外作为其它组分的微量成分附带地掺入组合物中的那些。
可用于本发明组合物实施方案中的载体包括水以及低级烷基醇和多元醇的水溶液。可用于本文的低级烷基醇是具有1至6个碳的一元醇，在一个方面，为乙醇和异丙醇。可用于本文的示例性多元醇包括丙二醇、己二醇、甘油和丙烷二醇。
清洁表面的方法
本发明涉及一种清洁上面存在包含脂肪酸的污垢的表面的方法，所述方法包括以下步骤：(a)使存在于所述表面上的所述污垢与消费产品组合物接触，所述消费产品组合物包含表面活性剂和选自以下的污垢转化酶：(i)α-双加氧酶；(ii)氢过氧脂肪酸生成酶，所述氢过氧脂肪酸生成酶选自脂氧合酶；和氢过氧脂肪酸转化酶，所述氢过氧脂肪酸转化酶选自环加氧酶(EC 1.14.99.1)、丙二烯氧化物合酶(EC 4.2.1.92)、氢过氧化物异构酶(EC4.2.1.92、EC 5.3.99.1、EC 5.4.4.5、EC 5.4.4.6)、氢过氧化物裂解酶(EC 4.2.1.92)、氢过氧化物脱水酶(EC 4.2.1.92)、二乙烯基醚合酶(EC 4.2.1.121、EC 4.2.1.B8、EC4.2.1.B9)、9,12-十八碳二烯酸8-氢过氧化物8R-异构酶(EC 5.4.4.5)、9,12-十八碳二烯酸8-氢过氧化物8S-异构酶(EC 5.4.4.6)、7,10-氢过氧化物二醇合酶、环氧醇合酶、以及它们的混合物，其中所述氢过氧脂肪酸转化酶能够转化所述氢过氧脂肪酸生成酶的反应产物；(iii)多结构域酶，所述多结构域酶包含氢过氧脂肪酸结构域，所述氢过氧脂肪酸结构域包括双加氧酶结构域和氢过氧脂肪酸转化结构域，所述氢过氧脂肪酸转化结构域选自丙二烯氧化物合酶结构域、环氧醇合酶结构域、氢过氧化物裂解酶结构域和氢过氧化物异构酶结构域；(iv)羟基脂肪酸生成酶，所述羟基脂肪酸生成酶选自过加氧酶、脂肪酸水合酶、以及它们的混合物；以及(iv)它们的混合物；和(b)将所述表面上的所述污垢的所述脂肪酸转化为选自氢过氧脂肪酸、氢过氧脂肪酸衍生物、羟基脂肪酸以及它们的混合物的活性脂肪酸衍生物物质。
本发明的方法可在表面上原位形成活性脂肪酸衍生物物质，从而在使用期间从消费产品组合物产生附加的清洁作用。这可提供增强的清洁有益效果和/或允许在消费产品组合物中使用降低的表面活性剂含量。
所述方法还可包括从所述表面除去所述消费产品组合物的步骤，例如通过从所述表面冲洗所述组合物(例如用水)或从所述表面机械地除去所述组合物(例如通过从所述表面擦拭组合物)。
所述方法还可包括用水稀释所述消费产品组合物以形成稀释的消费产品组合物，然后使所述表面与稀释的消费产品组合物接触的步骤。
用本发明的消费产品组合物处理的优选表面包括选自毛发、皮肤、织物、盘碟、餐具和家用硬质表面的表面。
在一个方面，污垢的脂肪酸选自单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、饱和脂肪酸、以及它们的混合物。优选地，污垢的脂肪酸选自肉豆蔻油酸、棕榈油酸、十六碳烯酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、鳕油酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物。优选地，污垢的脂肪酸选自棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物。优选地，污垢的脂肪酸是油酸。
在一个方面，通过所述方法形成的活性脂肪酸衍生物物质是选自以下的氢过氧脂肪酸：2-氢过氧脂肪酸、8R-氢过氧脂肪酸、8S-氢过氧脂肪酸、9R-氢过氧脂肪酸、9S-氢过氧脂肪酸、10R-氢过氧脂肪酸、11R-氢过氧脂肪酸、11S-氢过氧脂肪酸、12R-氢过氧脂肪酸、12S-氢过氧脂肪酸、13R-氢过氧脂肪酸、13S-氢过氧脂肪酸、14R-氢过氧脂肪酸、14S-氢过氧脂肪酸、15S-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；优选地选自2R-氢过氧脂肪酸、不饱和8R-氢过氧脂肪酸、不饱和9R-氢过氧脂肪酸、不饱和11R-氢过氧脂肪酸、不饱和12R-氢过氧脂肪酸、不饱和12S-氢过氧脂肪酸、不饱和13S-氢过氧脂肪酸、不饱和15S-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；并且更优选2R-氢过氧脂肪酸。
在一个方面，通过所述方法形成的活性脂肪酸衍生物物质是选自以下的氢过氧脂肪酸衍生物：二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇、以及它们的混合物；优选二羟基脂肪酸。
在一个方面，通过所述方法形成的活性脂肪酸衍生物物质是选自以下的羟基脂肪酸：2-羟基脂肪酸、3-羟基脂肪酸、4-羟基脂肪酸、5-羟基脂肪酸、6-羟基脂肪酸、以及它们的混合物；优选2-羟基脂肪酸、3-羟基脂肪酸、以及它们的混合物。
本发明还涉及一种清洁表面的方法，包括手动洗涤脏污制品，优选盘碟的方法，包括以下步骤：将本发明的组合物递送至一定体积的水中以形成洗涤溶液，以及将所述脏污制品浸入所述洗涤溶液中，其中所述脏污制品上的污垢包含至少一种选自以下的不饱和脂肪酸：单不饱和脂肪酸、二不饱和脂肪酸、三不饱和脂肪酸、四不饱和脂肪酸、五不饱和脂肪酸、六不饱和脂肪酸、以及它们的混合物。优选的不饱和脂肪酸包括：肉豆蔻油酸、棕榈油酸、十六碳烯酸、油酸、反油酸、异油酸、亚油酸、反亚油酸、α-亚麻酸、γ-亚麻酸、鳕油酸、α-桐酸、β-桐酸、蓖麻油酸、二十碳烯酸、花生四烯酸、二十碳五烯酸、芥酸、二十二碳二烯酸、二十二碳六烯酸、二十四碳烯酸、以及它们的混合物，更优选棕榈油酸、油酸、亚油酸、α-亚麻酸、γ-亚麻酸、以及它们的混合物，更优选油酸。
优选地，由脂肪酸与氢过氧脂肪酸生成酶的转化反应所形成的所得氢过氧脂肪酸选自8R-羟基过氧脂肪酸、8S-羟基过氧脂肪酸、9R-氢过氧脂肪酸、9S-氢过氧脂肪酸、10R-氢过氧脂肪酸、11R-氢过氧脂肪酸、11S-氢过氧脂肪酸、12R-氢过氧脂肪酸、12S-氢过氧脂肪酸、13R-氢过氧脂肪酸、13S-氢过氧脂肪酸、14R-氢过氧脂肪酸、14S-氢过氧脂肪酸、15S-氢过氧脂肪酸、它们的衍生物、以及它们的混合物；优选不饱和的8R-氢过氧脂肪酸、不饱和的8S-氢过氧脂肪酸、不饱和的9R-氢过氧脂肪酸、不饱和的10R-氢过氧脂肪酸、它们的衍生物、和它们的混合物。
由氢过氧脂肪酸转化酶转化氢过氧脂肪酸而形成的氢过氧脂肪酸衍生物优选选自单羟基脂肪酸、二羟基脂肪酸、环氧脂肪酸、氧代脂肪酸、二乙烯基醚脂肪酸、烯醛、醛、环氧醇、以及它们的混合物，优选二羟基脂肪酸。
优选地，在洗涤过程中，在含水洗涤液体中，氢过氧脂肪酸生成酶和氢过氧脂肪酸转化酶各自以0.005ppm至15ppm，优选0.01ppm至5ppm，更优选0.02ppm至0.5ppm的浓度存在。因此，本文的组合物将以其稀释形式施用到盘碟上。将脏污表面例如盘碟与有效量的，通常0.5mL至20mL(每25个待处理的盘碟)，优选3mL至10mL的稀释在水中的本发明洗涤剂组合物(优选液体形式)接触。洗涤剂组合物的实际用量将基于使用者的判断，并且将典型地根据以下因素：如，具体的组合物产品制剂，包括组合物中活性成分的浓度、待清洁的脏盘碟数量、盘碟脏的程度等。通常，将0.01mL至150mL，优选3mL至40mL的本发明的液体洗涤剂组合物与2,000mL至20,000mL，更典型地5,000mL至15,000mL的水在具有1,000mL至20,000mL，更典型地5,000mL至15,000mL范围内的体积容量的池中混合。将脏污盘碟浸入包含稀释组合物的池，然后用布、海绵或类似制品接触盘碟的脏污表面来实现清洁它们。可将布、海绵或类似制品在与盘碟表面接触之前浸入洗涤剂组合物和水的混合物中，然后典型地与盘碟表面接触1秒至10秒的时间，虽然实际时间将随每个应用和使用者的不同而不同。织物、海绵或类似制品与表面的接触优选伴随着表面的同时洗擦。
另选地，本发明的组合物可直接递送到盘碟上，或通过在在水的存在下用组合物清洁盘碟之前，使包含组合物的清洁工具(如海绵)与盘碟接触，以及任选地冲洗。此类直接应用的盘碟洗涤方法对于清洁油脂盘碟特别有益，尤其是在油脂已被烘烤的情况下。
本发明包括一种或多种氢过氧脂肪酸生成酶和一种或多种氢过氧脂肪酸转化酶在包含污垢的含水洗涤液体中提供增加的泡沫持久性的用途，其中所述污垢包含脂肪酸。酶优选包含在用于手动洗涤盘碟的洗涤剂组合物中，特别是本发明的洗涤剂组合物中。
本发明还涉及本文所述的消费产品组合物将存在于表面上的污垢的脂肪酸转化为活性脂肪酸衍生物物质的用途，所述活性脂肪酸衍生物物质选自氢过氧脂肪酸、氢过氧脂肪酸衍生物、羟基脂肪酸、以及它们的混合物。
测试方法
以下所述测定必须依次采用，使得本文描述并且受权利要求书保护的发明可被更完全理解。
测试方法1-玻璃小瓶泡沫里程(mileage)方法
玻璃小瓶泡沫里程测试方法的目的是测量在油脂污垢例如橄榄油存在下洗涤剂组合物的某种溶液产生的泡沫体积随时间的演变。此方法的步骤如下：
1.通过随后在室温下向40mL玻璃小瓶(尺寸：95mm H×27.5mm D)加入以下物质的等分试样来制备测试溶液：a)10g指定洗涤剂浓度和水硬度的含水洗涤剂溶液，b)1.0g指定浓度和水硬度的含水蛋白质(或蛋白质混合物)溶液，和c)0.11g橄榄油(
2.通过在磁力搅拌板(IKA，型号RTC B S001；VWR磁力搅拌器，目录号58949-012；500RPM)上在室温下搅拌2分钟，然后手动摇动20秒并向上向下移动(每秒约2个上下循环，+/-30cm向上和30cm向下)，将测试溶液在密闭的试验瓶中混合。
3.在摇动后，将密闭小瓶中的测试溶液在磁力搅拌板(IKA，型号RTC B S001；VWR磁力搅拌器，目录号58949-012；500RPM)上在46℃水浴中进一步搅拌60分钟，以保持恒定温度。然后如上所述手动摇动样品另外20秒，用尺子记录初始泡沫高度(H1)。
4.在46℃水浴中，在搅拌(IKA，型号RTC B S001型；VWR磁力搅拌器，目录号58949-012；500RPM)的同时，再将样品温育30分钟，然后如上所述手动摇动20秒。记录最终泡沫高度(H2)。
5.产生较大泡沫高度(H1和H2)的蛋白质溶液，优选与泡沫高度在H1和H2之间的较低液滴组合，是更理想的。
测试方法2-小水槽泡沫里程方法
当如下周期性地加入污垢负载时，可确定由液体洗涤剂组合物的溶液产生的泡沫体积的演变。将500mL液体洗涤剂组合物在15dH硬水中的溶液(最终浓度为0.12重量％，初始温度为46℃)的等分试样添加到圆柱形容器(尺寸：150mm D×150mm H)中。在测试期间将容器在水浴中温育以将溶液的温度保持在46℃和40℃之间。通过用位于容器中部的桨(尺寸：50mm×25mm)以135rpm机械搅拌120秒，在容器中产生初始泡沫体积。
然后，用20mL注射器和自动泵(KDS Legato110单注射器I/W泵)将0.5mL的油脂污垢的等分试样(组成：参见表3，0.5mL)添加到溶液中，同时桨以135rpm旋转至溶液中，持续14秒。混合后，在下一个循环之前将溶液再温育166秒。每180秒重复污垢注射、桨划和温育步骤，直到达到终点，并记录所需的污垢添加量。当连续两次或更多次观察到环绕叶轮至少半周的透明无泡沫环时，出现终点。将整个过程重复多次，并且计算每种液体洗涤剂组合物的所有平行测定的添加数的平均值。
最后，然后如下计算泡沫里程指数：(测试液体洗涤剂组合物的污垢添加的平均数)/(参考液体洗涤剂组合物的污垢添加的平均数)×100。根据测试目的，本领域的技术人员可以选择替代的水硬度、溶液温度、产品浓度或污垢类型。
表3-油脂污垢组合物
测试方法3-大水槽泡沫里程方法
当如下周期性地加入污垢负载时，可确定由洗涤剂组合物的溶液产生的泡沫体积的演变。用4巴的恒压将硬水(15dH)流填充到水槽(圆筒尺寸：300mm D×288mm H)中至4L。同时，通过移液管以0.67mL/秒的流速在水槽表面底部上方37cm的高度处分配洗涤剂组合物的等分试样(最终浓度0.12重量％)。由于水的压力，在水槽中产生初始泡沫体积。在测试期间，将溶液的温度保持在46℃。
在记录初始泡沫体积(平均泡沫高度×水槽表面积)后，将固定量的油脂污垢(组成：参见表3，4mL)注入水槽的中部，同时桨(尺寸：10cm×5cm，以45度角定位在水槽的中部，处于气液界面处)以85rpm旋转20次到溶液中。该步骤后立即进行另一次总泡沫体积的测量。重复污垢注射、桨划和测量步骤，直到测得的泡沫体积达到设定为400cm3的最小水平。记录达到该水平所需的污垢添加量。将整个过程重复多次，并且计算每种洗涤剂组合物的所有平行测定的添加数的平均值。
最后，然后如下计算泡沫里程指数：(测试洗涤剂组合物的污垢添加的平均数)/(参考洗涤剂组合物的污垢添加的平均数)×100。
根据测试目的，本领域的技术人员可以选择替代的水硬度、溶液温度、产品浓度或污垢类型。
实施例
提供以下实施例以进一步说明本发明，而非解释为是对本发明的限制，因为在不脱离本发明实质或范围的条件下可以对本发明进行许多改变。
实施例1a-铜绿假单胞菌菌株42A2 10S-DOX
铜绿假单胞菌10S-DOX(SEQ ID NO:18)是氢过氧脂肪酸生成酶(油酸10S-脂氧合酶，EC 1.13.11.77)，该氢过氧脂肪酸生成酶将不饱和脂肪酸(例如油酸和亚油酸)转化为对应的氢过氧化物质，并且其被包括作为本发明的一部分。设计并合成编码铜绿假单胞菌菌株42A2 10S-DOX变体的密码子优化基因(SEQ ID NO:55)，包括含有His-tag、MBP标签和TEV蛋白酶切割位点的N-末端氨基酸序列(SEQ ID NO:56)。基因合成后，用Genscript(Piscataway,NJ)表达和纯化该蛋白质。简言之，将完整的合成基因序列亚克隆到pET28a载体中以进行异源表达。用重组质粒转化大肠杆菌BL21(DE3)细胞，并将单菌落接种到含卡那霉素的LB培养基中。将培养物在15℃下以200rpm温育16小时，并加入异丙基β-D-1-硫代半乳糖苷(IPTG)(最终浓度1mM)以诱导蛋白质表达。通过离心收获细胞，并通过超声处理来裂解沉淀。离心后，收集上清液，并使用镍亲和柱和本领域已知的标准工序通过一步纯化来纯化蛋白质。将蛋白质储存在含有50mM Tris-HCl、150mM NaCl和10％甘油的pH 8.0的缓冲液中。通过Bradford蛋白质测定以BSA为标准(ThermoFisher，目录号23236)进行测定，最终的蛋白质浓度为0.12mg/mL。
实施例1b-点形念珠藻HPL
点形念珠藻HPL(SEQ ID NO:40)是将氢过氧化物脂肪酸(例如10S-氢过氧亚油酸酯)转化为较小脂肪酸和醇并且被包括作为本发明的一部分的酶(氢过氧化物裂解酶，EC4.2.1.92)。设计并合成编码点形念珠藻HPL变体的密码子优化基因(SEQ ID NO:62)，包括含有His-tag、MBP标签和TEV蛋白酶切割位点的N-末端氨基酸序列(SEQ ID NO:63)。基因合成后，用Genscript(Piscataway,NJ)表达和纯化该蛋白质。简言之，将完整的合成基因序列亚克隆到pET28a载体中以进行异源表达。用重组质粒转化大肠杆菌BL21(DE3)细胞，并在37℃下将单菌落接种到含卡那霉素的TB培养基中。当OD600达到约0.8-1.0时，通过添加异丙基β-D-1-硫代吡喃半乳糖苷(IPTG)(最终浓度0.1mM)和δ-氨基乙酰丙酸(最终浓度0.25mM)诱导蛋白质表达。将培养物在16℃下以200rpm培养16小时。通过离心收获细胞，并通过超声处理来裂解沉淀。离心后，收集上清液，并使用镍亲和柱和本领域已知的标准工序通过两步纯化来纯化蛋白质。将蛋白质储存在含有10％甘油的1X PBS缓冲液(pH 7.4)中。通过Bradford蛋白质测定以BSA为标准(ThermoFisher，目录号23236)进行测定，最终的蛋白质浓度为1.58mg/mL，通过在还原条件下考马斯蓝染色的SDS-PAGE凝胶的光密度分析估计，纯度为约75％。
实施例1c-氢过氧脂肪酸生成和转化酶洗涤剂组合物
在特定条件(例如，水硬度、溶液温度、洗涤剂浓度等)下，跟踪在污垢(即，橄榄油或油脂污垢)存在下由洗涤剂组合物的特定溶液所产生的泡沫体积的演变。制备下列溶液：
A.硬水(15dH)：将0.75g MgCl2.6H2O(Sigma-Aldrich，目录号M9272)、2.10gCaCl2.6H2O(Sigma-Aldrich，目录号21108)和0.689g NaHCO3(Sigma-Aldrich，目录号31437)溶解在5L去离子水中。
B.对照参考洗涤剂组合物的洗涤剂溶液(“溶液DG-R”)使用Fairy Dark Green(2017年2月在英国可商购获得)制备，稀释在上述制备的硬水(15dH)中，目标洗涤剂浓度为0.12％。
C.蛋白质溶液：在进行泡沫里程方法之前，将蛋白质在去离子水中稀释至所需浓度。
D.油脂污垢：根据表3中所述的组合物制备油脂污垢。
实施例2：示例性手动盘碟洗涤洗涤剂组合物
表5中示出包含根据本发明的a)氢过氧脂肪酸生成酶铜绿假单胞菌菌株42A210S-DOX(SEQ ID NO:18)和b)一种或多种氢过氧化脂肪酸转化酶铜绿假单胞菌菌株42A27,10-DS/HP-异构酶(SEQ ID NO:38)或点形念珠藻HPL(SEQ ID NO:40)的手动盘碟洗涤洗涤剂组合物。酶可以按照实施例1a和1b中描述的方案或本领域中描述的类似方法来制备(Estupinan,M.等人(2015)).PLoS One 10(7):e0131462/0131461-e0131462/0131420)。
表5：洗涤剂组合物
自动盘碟洗涤组合物的实施例
下列是自动盘碟洗涤组合物形式的本发明消费产品组合物的非限制性实施例。
其中上表中的值以活性物质的克数给出。
淀粉酶 由Novozymes提供的
漂白活化剂 Weylchem提供的PAAN
Lutensol TO7 由BASF提供的非离子表面活性剂
液体聚合物 由Nippon Shokubi提供的GT 101
MGDA 由BASF提供的甲基甘氨酸二乙酸三钠
蛋白酶 由DuPont提供的
磺化聚合物 由Dow Chemicals提供的Acusol 588
SLF180 由BASF提供的非离子表面活性剂
洗发剂组合物的实施例
下列是用于清洁毛发的洗发剂组合物形式的本发明消费产品组合物的非限制性实施例。
1衍生自具有低含水粘度的天然胶的阳离子聚合物
2阳离子合成共聚物
3植物来源的阳离子聚合物
实施例17至22：用于手洗或洗衣机(通常为顶部加载式洗衣机)的颗粒状衣物洗涤剂组合物。
实施例23-28：通常用于前加载式自动洗衣机的颗粒状衣物洗涤剂组合物。
实施例29-35：重垢型液体衣物洗涤剂组合物。
基于总清洁和/或处理组合物重量。将酶水平报告为原料。
实施例36至47：单位剂量组合物
这些实施例提供了用于单位剂量衣物洗涤剂的各种配方，并且包括包含一个粉末隔室和一个液体隔室的双隔室单位剂量产品。用于包封组合物的膜为PVA。通过将选自组合物A-E的液体隔室组合物与选自组合物F-K的粉末隔室组合物组合来制备每个实施例。
下列实施例A-E是单位剂量产品的液体组合物：
下列实施例F-K是单位剂量产品的粉末/固体组合物：
基于总清洁和/或处理组合物/隔室重量。将酶水平报告为原料。
对于上述实施例17-47，适用以下定义：
AE1.8S为C12-15烷基乙氧基(1.8)硫酸盐
AE3S为C12-15烷基乙氧基(3)硫酸盐
AE7 为C12-13醇乙氧基化物，平均乙氧基化度为7
AE8 为C12-13醇乙氧基化物，平均乙氧基化度为8
AE9 为C12-13醇乙氧基化物，平均乙氧基化度为9
淀粉酶1 为
淀粉酶2 为
淀粉酶3 为
AS为C12-14烷基硫酸盐
纤维素酶2 为CellucleanTM，15.6mg活性物质/g
木葡聚糖酶为
螯合剂1 为二亚乙基三胺五醋酸
螯合剂2 为1-羟乙烷1,1-二膦酸
螯合剂3 为乙二胺-N,N′-二琥珀酸的钠盐，(S,S)异构体(EDDS)
分散体B 为糖苷水解酶，报告为1000mg活性物质/g
DTI 1 为聚(4-乙烯基吡啶-1-氧化物)(诸如
DTI 2 为聚(1-乙烯基吡咯烷酮-共-1-乙烯基咪唑)(诸如
HSAS为中间支化的烷基硫酸盐，如US 6,020,303和US 6,060,443中公开的
LAS 为具有C9-C15平均脂族碳链链长的直链烷基苯磺酸盐(HLAS为酸形式)。
脂肪酶为
甘露聚糖酶为
核酸酶为磷酸二酯酶SEQ ID NO 1，报告为1000mg活性物质/g
光学增白剂1 为4,4′-双{[4-苯胺基-6-吗啉基-均-三嗪-2-基]-氨基}-2,2′-二苯乙烯二磺酸二钠
光学增白剂2 为4,4′-双-(2-磺基苯乙烯基)联苯二钠(钠盐)
光学增白剂3 为来自3V Sigma的
香料胶囊包封物为核-壳三聚氰胺甲醛香料微胶囊
光漂白剂为磺化酞菁锌
抛光酶为对硝基苄基酯酶，报告为1000mg活性物/g
如本公开中所述的聚醚胺。
聚合物1 为双((C2H5O)(C2H4O)n)(CH3)-N+-CxH2x-N+-(CH3)-双((C2H5O)(C2H4O)n)，其中n＝20至30，x＝3至8，或其硫酸化或磺酸化变体
聚合物2 为乙氧基化(EO15)的四亚乙基五胺
聚合物3 为乙氧基化的聚乙烯亚胺
聚合物4 为乙氧基化的六亚甲基二胺
聚合物5 为Acusol 305，由Rohm&Haas提供
聚合物6 为接枝有乙酸乙烯酯侧链的聚乙二醇聚合物，由BASF提供。
蛋白酶为
蛋白酶2 为
蛋白酶3 为
季铵为C12-14二甲基羟乙基氯化铵
S-ACMC为由Megazyme提供的活性蓝19偶氮-CM-纤维素
去垢剂为
结构剂为氢化蓖麻油
紫罗兰DD为由Milliken提供的噻吩偶氮聚合物调色染料
针对酶给出的所有百分比和比率均基于活性蛋白质。除非另外指明，本文所有的百分比和比率都按重量计算。除非另外指明，否则所有百分比和比率均基于总组合物计算。
应当理解，贯穿本说明书给出的每一最大数值限度包括每一较低数值限度，如同此类较低数值限度在本文中明确写出。贯穿本说明书给出的每一最小数值限度将包括每一较高数值限度，如同此类较高数值限度在本文中明确写出。贯穿本说明书给出的每一数值范围将包括落在此类较宽数值范围内的每一较窄数值范围，如同此类较窄的数值范围全部在本文中明确写出。
本文所公开的量纲和值不应理解为严格限于所引用的精确数值。相反，除非另外指明，否则每个此类量纲旨在表示所述值以及围绕该值功能上等同的范围。例如，公开为“40mm”的量纲旨在表示“约40mm”。
序列表
<110> 宝洁公司（Procter & Gamble Company）
Velasquez, Juan E.
Gonzales, Denis A.
Bettiol, Jean-Luc P.
Geary, Nicholas W.
<120> 清洁具有包含脂肪酸的污垢的表面的方法
以及用于该方法的消费产品组合物
<130> 15408
<160> 80
<170> PatentIn 3.5版
<210> 1
<211> 1165
<212> PRT
<213> 小麦全蚀病菌（Gaeumannomyces graminis）
<400> 1
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Leu Arg Asp Leu Leu His His Val Phe Gly Asn Gln Lys Ser Pro Thr
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Val Tyr Pro Asn Ala Pro Gly Asn Ser Ala Lys Pro Val Pro Thr Gly
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Leu Ile Phe Leu Asn Ser Ala Val Lys Gly Val Asn Asp Asp Gln Gln
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Ser Arg Glu Gly Lys Lys Leu Thr Asp Gly Leu Ile Asn Asp Leu Trp
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Asp Ser Leu Asp His Pro Pro Val Ala Ser Leu Gly Lys Gly Phe Ser
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Gly Ala Ala Asn Thr Pro Tyr Ala Arg Ser Thr Lys Pro Leu Val Phe
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Gln Asn Pro Asn Pro Pro Asp Pro Ala Thr Ile Phe Asp Thr Leu Met
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Val Arg Asp Pro Ala Lys Phe Arg Pro His Pro Asn Lys Ile Ser Ser
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Met Leu Phe Tyr Leu Ala Thr Ile Ile Thr His Asp Ile Phe Gln Thr
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Ser Pro Leu Tyr Gly Arg Asn His Asp Glu Gln Met Ala Val Arg Thr
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Asp Lys Ala Val Asp Trp Gly His Val Ile Tyr Lys Leu Phe Phe Arg
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Ala Phe Pro Asn His Phe Leu Pro Asn Ser Val Tyr Ala His Phe Pro
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Phe Val Val Pro Ser Glu Asn Lys Leu Ile Phe Glu Gly Leu Gly Ala
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Ala Asn Lys Tyr Ser Trp Asp Pro Pro Lys Ala Arg Ala Pro Ile Gln
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Phe Ile Arg Ser His Lys Ala Val Leu Glu Val Leu Ser Asn Gln Lys
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Asp Tyr Lys Val Thr Trp Gly Pro Ala Ile Lys Met Leu Ser Gly Asp
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Ser Arg His His Val Ile Ala Ala Leu Thr Ala Pro Lys Gln Trp Arg
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Ala Ala Ile Phe Trp Asp Ser Asp Val Cys Asn Ser Leu Lys Leu His
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Gln Ala Ser Lys Ala Ala Ala Asp Lys Met Ser Ala Leu Ile Ala Glu
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His Val Arg Glu Met Glu Ala Gly Thr Gly Phe Leu Gly Ala Leu Gly
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His Thr Asn Gly Asn Gly Val His Thr Asn Gly Asn Gly Val Pro His
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Met Lys Arg Leu Ala Met Leu Asn Thr Ser Glu Ala Asp Glu Lys Leu
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<210> 2
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<213> 念珠藻属(Nostoc sp.)
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Asn Pro Asp Ser Phe Thr Glu Leu Tyr Tyr His Thr Gln Leu Cys Tyr
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Asn Ile Val Glu Tyr Val Leu Gln Ala Gln Phe Arg Ser Ile Glu Asp
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Val Ala Val Asp Cys Ser Asn Ile Trp Asp Pro Asn Thr Tyr Pro Trp
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Leu Asp Ile Ala Ala Ile Val Leu Asn Gln Asp Glu Ser Glu Asn Asp
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Tyr Tyr Gln Glu Ile Ala Tyr Asn Pro Gly Asn Thr His Tyr Asp Leu
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Tyr Phe Pro Leu Pro Val Thr Glu Ile Pro Ser Lys Arg Phe Leu Phe
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Leu Leu Glu Lys Tyr Asn Phe Leu Thr Asp Asn Ser Tyr Pro Ser Asp
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Gly Glu His Asp Lys Ile Glu Ala Leu Val Ser Ala Met Pro Thr Thr
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Ala Leu Asp Leu Ala Val Gly Thr Thr Asp Pro Thr Asp Ile Pro Asp
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Ser Tyr Phe Leu Glu Arg Arg Leu Asn Gly Tyr Asn Pro Gly Ala Ile
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Arg Glu Ser Ser Gly Gln Glu Gly Trp Thr His Glu Leu Thr His Asn
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Gln Cys Arg Leu Phe Val Asp Lys Gln Asn Gly Val Lys Leu His Ser
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Ile Lys Ile Asp Asp His Glu Ile Thr Pro Cys Gln Glu Gln Trp Gln
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Ala Ile Lys Arg Arg Leu Ala Pro Thr His Pro Val Arg Ala Phe Ile
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Asn Pro His Leu Glu Gly Leu Ile Phe Ile Asn Ser Ser Ala Val Pro
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Lys Ile Ile Gly Ser Thr Gly Phe Ile Pro Ile Ala Ser Met Leu Thr
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Gln Gly Ser Ile Val Asp Val Met Lys Asn Glu Leu Ser Lys Leu Ser
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Tyr Met Trp Asn Pro Ile Ala Asp Leu Pro Arg Asp Ile Pro Gly Asp
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Ser Leu Tyr Ser Gly Asp Gln Pro Pro Lys Tyr Asp Ser Ser Glu Leu
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Lys Ser Leu Leu Met Tyr Ile Ile Tyr His Ser Ser Phe Leu His Ser
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Trp Ala Asn Phe Lys Gln Tyr Asp Asp Ala Gly Asn Pro Asn His Val
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Ser Met Gly Asp Tyr Ser Gln Tyr Asp Gln Gln Thr Gln Asp Lys Ile
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Arg Phe Ser Gln Arg Ser Leu Thr Trp Val Leu Ser Ser Ile Arg Tyr
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Asn Ser Val Ala Val Tyr Gly Ser Asp Leu Leu Lys Gln Leu Ile Arg
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Glu Lys Ser Ser Ile Leu Glu Pro Gly Leu Pro Leu Glu Asp Leu Met
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Met Ser Ile Asn Ile
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<210> 3
<211> 805
<212> PRT
<213> 深海单细胞蓝细菌（Acaryochloris marina）
<400> 3
Met Asp Ser Arg Asp Pro Arg Thr Glu His Val Asp Asp Glu Phe Lys
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Ala Ala Met Leu Gln Glu Phe Pro Lys Ile Ala Pro Ile Phe His Glu
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Ile Ile Arg Asp Pro Asp Ser Tyr Thr Gln Leu His Tyr Tyr Ser Glu
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Tyr Arg Leu Val Asn Arg Gln Asn Pro Ser Ala Asp Thr Gly Trp Leu
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Lys Pro Glu Glu Val Lys Leu Pro Leu Asp Tyr Leu Pro Arg Val Ala
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Ser Asp Thr Arg Pro Glu Thr Tyr Leu Gln Asp Asp Phe Arg Gln Gln
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Val Arg Ser Thr Gly Val Ser Tyr Leu Leu Gln Ile Gln Leu Gln Pro
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Val Ser Asp Asp Ala Ala Met Asn Glu Thr Ala Lys Asp Cys Thr Ile
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Pro Trp Glu Glu Glu Asp His Pro Phe His Asp Val Ala Val Leu Asp
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Leu Gly Ser Ile Leu Pro Asp Glu Leu Ala Glu Ala Leu Glu Phe Asn
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Pro Tyr Asn Ala Pro Pro Glu Leu Ser Leu Ile Leu Ala Lys Thr Ala
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Arg Glu Thr Ala Ser Val Asn His Leu Arg Ser Val Val Tyr Gln Ile
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Ser Ala Asn Met Arg Lys Tyr Gln Thr Pro Ser Ser Ser Leu Val Asp
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Trp Gly Ser Gly His Gln Pro Ser Leu Pro Glu Gln Tyr Pro Tyr Gly
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Thr Gly Lys Thr Pro Ser Phe Asp Asn Thr Lys Pro Leu Pro Ala Arg
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Val Lys Pro Lys Pro Arg Tyr Trp Ala Asn Phe Gly Leu Lys Leu Ile
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Pro Asn Gln Gln Leu Asp Pro Asp Leu Pro Glu Leu Gly Ile Thr Gly
405 410 415
Ala Leu Asp Leu Met Gly Thr Ser Val Val Ser Tyr Met Pro Pro Asn
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Leu Thr Arg Thr Arg Leu Asp Lys Phe Ser Asp Asp Phe Phe Val Glu
435 440 445
Arg Arg Leu Asn Gly Phe Asn Pro Gly Lys Leu Asn Arg Val Thr Gly
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His Ala Trp Gln Tyr Gln Val Cys Tyr Asp Cys Ser Lys His Gln Val
465 470 475 480
Glu Pro Ala Gly Ile Leu Pro Thr Lys Ile Thr Ala Arg Phe Asn Phe
485 490 495
Cys Gly Gln Tyr Leu His Pro His Ser Ile Gln Phe Thr Leu Asn Gly
500 505 510
Gln Thr Glu Thr Gln Gln Pro Gly Asp Glu Asn Trp Glu Trp Ser Lys
515 520 525
Arg Leu Phe Arg Cys Ala Glu Phe Val Phe Gln Glu Ala Gln Ser His
530 535 540
Leu Gly Arg Thr His Met Asn Leu Asp Gln Tyr Ala Met Ala Tyr Tyr
545 550 555 560
Arg Asn Val Val Asn Asn Pro Ile Arg Leu Leu Leu Glu Pro His Leu
565 570 575
Glu Gly Leu Leu Ser Ile Asn Lys Leu Gly Ala Asn Leu Ile Ser Gly
580 585 590
Pro Thr Gly Phe Ile Pro Glu Ala Ser Ser Leu Thr Pro Glu Ser Val
595 600 605
Asp Asp Val Leu Lys Asp Glu Ile Ser His Leu Ser Tyr His Trp Thr
610 615 620
Pro His Arg Gln Thr Leu Pro Asp Arg Val Leu Asn Asn His Tyr Asp
625 630 635 640
Pro Ala Ala Ile Ala Met Trp Asn Leu Leu Thr Gln Tyr Val Arg Glu
645 650 655
Phe Phe Glu Asp His Gln Ala Gly Met Glu Glu Tyr Trp Ser Glu Ile
660 665 670
Gln Ala Met Ser His Asp Leu Val Thr His Ser Ile Leu Lys Pro Glu
675 680 685
Leu Gly Thr Leu Ala Val Gln Asn Asn Ala Asp Leu Gln Gln Leu Cys
690 695 700
Val Tyr Val Ile Phe Leu Ser Ser Phe Phe His Ser Trp Val Asn Asn
705 710 715 720
Lys Gln Tyr Glu Asp Gly Gly Asp Val Ser Tyr Ser Thr Ile Gly Leu
725 730 735
Trp Asp Thr Arg His Pro Lys Tyr Asp Pro Leu Arg Val Ala Glu Arg
740 745 750
Glu Ala Lys Gln Val Thr Leu Leu Trp Thr Leu Ser His Val Arg Tyr
755 760 765
Asn Pro Ile Met Asp Val Gly Pro Thr Ala Leu Lys Asn Leu Leu Trp
770 775 780
Gln Gln Arg Gln His Ile Glu Pro Gly Ile Pro Leu Ala Asn Leu Met
785 790 795 800
Met Ser Thr Asn Ile
805
<210> 4
<211> 862
<212> PRT
<213> 燕麦（Avena sativa）
<400> 4
Met Leu Leu Gly Gly Leu Ile Asp Asn Leu Thr Gly Ala Asn Lys His
1 5 1015
Ala Arg Leu Lys Gly Thr Val Val Leu Met Arg Lys Asn Val Leu Asp
202530
Leu Asn Asp Phe Gly Ala Thr Ile Ile Asp Gly Val Ser Glu Phe Leu
354045
Gly Lys Gly Val Thr Cys Gln Leu Ile Ser Ser Thr Val Val Asp Asn
505560
Asn Asn Gly Asn Arg Gly Lys Val Gly Ala Glu Ala Gly Leu Glu Gln
65707580
Trp Leu Thr Ser Leu Pro Ser Leu Thr Thr Gly Glu Ser Lys Phe Gly
859095
Leu Thr Phe Asp Trp Glu Val Asp Lys Leu Gly Val Pro Gly Ala Ile
100 105 110
Val Val Asn Asn Tyr His Ser Ala Gln Phe Phe Leu Lys Thr Ile Thr
115 120 125
Leu Asp Asp Val Pro Gly Arg Ala Gly Lys Leu Thr Phe Val Ala Asn
130 135 140
Ser Trp Ile Tyr Pro Ala Glu Lys Tyr Arg Tyr Asn Arg Val Phe Phe
145 150 155 160
Ala Asn Asp Thr Tyr Leu Pro Ser Gln Met Pro Ala Ala Leu Lys Pro
165 170 175
Tyr Arg Asp Asp Glu Leu Arg Asn Leu Arg Gly Asp Asp Gln Gln Gly
180 185 190
Pro Tyr Glu Glu His Asp Arg Val Tyr Arg Tyr Asp Val Tyr Asn Asp
195 200 205
Leu Gly Glu Asp Arg Pro Val Leu Gly Gly Thr Ala Asp His Pro Tyr
210 215 220
Pro Arg Arg Gly Arg Thr Gly Arg Lys Pro Asn Pro Asn Asp Pro Ser
225 230 235 240
Leu Glu Ser Arg Leu Ser Leu Leu Glu Gln Ile Tyr Val Pro Arg Asp
245 250 255
Glu Lys Phe Gly His Leu Lys Met Ser Asp Phe Leu Gly Tyr Ser Ile
260 265 270
Lys Ala Ile Thr Gln Gly Ile Leu Pro Ala Val Arg Thr Tyr Val Asp
275 280 285
Cys Thr Pro Gly Glu Phe Asp Ser Phe Gln Asp Ile Ile Asn Leu Tyr
290 295 300
Glu Gly Gly Ile Lys Leu Pro Lys Ile Ala Ala Leu Glu Glu Leu Arg
305 310 315 320
Lys Arg Phe Pro Phe Gln Leu Leu Lys Asp Leu Leu Pro Val Gly Gly
325 330 335
Asp Phe Leu Leu Lys Leu Pro Leu Pro His Ile Ile Lys Glu Asp Lys
340 345 350
Gln Ala Trp Arg Thr Asp Glu Glu Phe Ala Arg Glu Val Leu Ala Gly
355 360 365
Val Asn Pro Val Met Ile Thr Arg Leu Thr Glu Phe Pro Pro Lys Ser
370 375 380
Thr Leu Asp Pro Ser Lys Tyr Gly Asp His Thr Ser Thr Ile Thr Ala
385 390 395 400
Ala His Ile Glu Lys Asn Leu Glu Gly Leu Thr Val Gln Gln Ala Leu
405 410 415
Glu Gly Asn Lys Leu Tyr Ile Leu Asp His His Asp Arg Phe Met Pro
420 425 430
Phe Leu Ile Asp Val Asn Asn Leu Asp Gly Asn Phe Ile Tyr Ala Thr
435 440 445
Arg Thr Leu Phe Phe Leu Arg Gly Asp Gly Arg Leu Thr Pro Leu Ala
450 455 460
Ile Glu Leu Ser Glu Pro Phe Ile Gln Asp Gly Leu Thr Thr Ala Lys
465 470 475 480
Ser Lys Val Tyr Thr Pro Val Pro Ser Gly Ser Val Glu Gly Trp Val
485 490 495
Trp Glu Leu Ala Lys Ala Tyr Val Ala Val Gly Asp Ser Gly Trp His
500 505 510
Gln Leu Val Ser His Trp Leu Asn Thr His Ala Val Met Glu Pro Phe
515 520 525
Val Ile Ala Thr Asn Arg Gln Leu Ser Val Thr His Pro Val His Lys
530 535 540
Leu Leu Ser Pro His Tyr Arg Asp Thr Met Thr Ile Asn Ala Leu Ala
545 550 555 560
Arg Gln Thr Leu Ile Asn Ala Gly Gly Ile Phe Glu Met Thr Val Phe
565 570 575
Pro Gly Lys Phe Ala Leu Gly Met Ser Ser Val Val Tyr Lys Asp Trp
580 585 590
Asn Phe Ala Glu Gln Gly Leu Pro Asp Asp Leu Leu Arg Arg Gly Val
595 600 605
Ala Val Pro Asp Pro Ser Ser Pro Tyr Lys Val Arg Leu Leu Ile Glu
610 615 620
Asp Tyr Pro Tyr Ala Ala Asp Gly Leu Ala Ile Trp His Ala Ile Glu
625 630 635 640
Gln Tyr Val Thr Glu Tyr Leu Ala Ile Tyr Tyr Pro Asp Asp Ala Val
645 650 655
Leu Gln Asp Asp Val Glu Leu Gln Ala Trp Trp Lys Glu Ala Arg Glu
660 665 670
Val Gly His Gly Asp Leu Lys Asp Ala Pro Trp Trp Pro Ser Met Gln
675 680 685
Thr Val Ala Glu Leu Ala Lys Ser Cys Ala Thr Ile Ile Trp Ile Ala
690 695 700
Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln Tyr Pro Tyr Ala Gly
705 710 715 720
Tyr Leu Pro Asn Arg Pro Thr Val Ser Arg Arg Arg Met Pro Glu Pro
725 730 735
Gly Thr Gln Glu Tyr Ala Glu Leu Glu Arg Asp Pro Glu Arg Ala Phe
740 745 750
Ile His Thr Ile Thr Ser Gln Ile Gln Thr Ile Ile Gly Ile Ser Leu
755 760 765
Leu Glu Val Leu Ser Lys His Ser Ser Asp Glu Leu Tyr Leu Gly Gln
770 775 780
Arg Asp Thr Pro Glu Trp Thr Ser Asp Pro Lys Ala Leu Ala Val Phe
785 790 795 800
Gln Arg Phe Ser Asp Arg Leu Val Asp Ile Glu Ser Lys Val Val Gly
805 810 815
Met Asn His Asp Pro Gln Leu Lys Asn Arg Asn Gly Pro Ala Lys Leu
820 825 830
Pro Tyr Met Leu Leu Tyr Pro Asn Thr Ser Asp Arg Lys Gly Asp Ala
835 840 845
Ala Gly Leu Thr Ala Lys Gly Ile Pro Asn Ser Ile Ser Ile
850 855 860
<210> 5
<211> 863
<212> PRT
<213> 水稻(Oryza sativa)
<400> 5
Met Leu Gly Gly Leu Lys Asp Lys Leu Thr Gly Lys Asn Gly Asn Lys
1 5 1015
Ile Lys Gly Leu Ala Val Leu Met Ser Arg Lys Leu Leu Asp Pro Arg
202530
Asp Phe Thr Ala Ser Leu Leu Asp Asn Val His Glu Val Phe Gly Asn
354045
Ser Ile Thr Cys Gln Leu Val Ser Ala Thr Val Ala Asp Gln Asn Asn
505560
Glu Gly Arg Gly Ile Val Gly Ser Glu Ala Asn Leu Glu Gln Gly Leu
65707580
Thr Asp Leu Pro Ser Val Ser Gln Gly Glu Ser Lys Leu Thr Val Arg
859095
Phe Asn Trp Glu Met Asp Lys His Gly Val Pro Gly Ala Ile Ile Ile
100 105 110
Lys Asn His His Ser Thr Lys Phe Phe Leu Lys Thr Ile Thr Leu His
115 120 125
Asp Val Pro Gly Cys Asp Thr Ile Val Phe Val Ala Asn Ser Trp Ile
130 135 140
Tyr Pro Val Gly Lys Tyr His Tyr Asn Arg Ile Phe Phe Ala Asn Asn
145 150 155 160
Ser Tyr Leu Pro Ser Gln Met Pro Glu Ala Leu Arg Pro Tyr Arg Glu
165 170 175
Asp Glu Leu Arg Tyr Leu Arg Gly Glu Asp Arg Gln Gly Pro Tyr Gln
180 185 190
Glu His Asp Arg Ile Tyr Arg Tyr Asp Val Tyr Asn Asp Leu Gly Glu
195 200 205
Pro Asp Arg Asp Asn Pro Arg Pro Val Leu Gly Gly Ser Gln Lys His
210 215 220
Pro Tyr Pro Arg Arg Gly Arg Thr Gly Arg Ile Pro Thr Lys Lys Asp
225 230 235 240
Pro Asn Ser Glu Ser Arg Leu Ser Leu Leu Glu Gln Ile Tyr Val Pro
245 250 255
Ser Asp Glu Arg Phe Ala His Leu Lys Met Ser Asp Phe Ala Gly Tyr
260 265 270
Ser Ile Lys Ala Ile Val Gln Gly Ile Leu Pro Ala Ile Arg Thr Tyr
275 280 285
Val Asp Leu Thr Pro Gly Glu Phe Asp Ser Phe Glu Asp Ile Leu Lys
290 295 300
Leu Tyr Arg Gly Gly Leu Lys Leu Pro Ser Ile Pro Ala Leu Glu Glu
305 310 315 320
Leu Arg Lys Ser Phe Pro Val Gln Leu Ile Lys Asp Leu Leu Pro Val
325 330 335
Gly Gly Ser Tyr Leu Leu Lys Phe Pro Lys Pro Asp Ile Ile Lys Glu
340 345 350
Asn Glu Val Ala Trp Arg Thr Asp Glu Glu Phe Ala Arg Glu Ile Leu
355 360 365
Ala Gly Leu Asn Pro Met Val Ile Arg Arg Leu Thr Glu Phe Pro Pro
370 375 380
Lys Ser Thr Leu Asp Pro Ser Lys Tyr Gly Asp Gln Thr Ser Thr Ile
385 390 395 400
Thr Pro Ala His Ile Glu Lys Asn Leu Glu Gly Leu Ser Val Gln Gln
405 410 415
Ala Leu Asp Ser Asn Arg Leu Tyr Ile Leu Asp His His Asp His Phe
420 425 430
Met Pro Phe Leu Ile Asp Ile Asn Ser Leu Asp Gly Ile Phe Thr Tyr
435 440 445
Ala Thr Arg Thr Leu Leu Phe Leu Arg Asp Asp Asp Thr Leu Lys Pro
450 455 460
Leu Ala Ile Glu Leu Ser Leu Pro His Ile Glu Gly Asn Leu Thr Ser
465 470 475 480
Ala Lys Ser Lys Val His Thr Pro Ala Ser Ser Gly Ile Glu Ser Trp
485 490 495
Val Trp Gln Leu Ala Lys Ala Tyr Val Ala Val Asn Asp Ser Gly Trp
500 505 510
His Gln Leu Ile Ser His Trp Leu Asn Thr His Ala Val Met Glu Pro
515 520 525
Phe Val Ile Ala Thr Asn Arg Gln Leu Ser Val Thr His Pro Val Tyr
530 535 540
Lys Leu Leu Gln Pro His Tyr Arg Asp Thr Met Thr Ile Asn Ala Leu
545 550 555 560
Ala Arg Gln Thr Leu Ile Asn Gly Gly Gly Ile Phe Glu Gln Thr Val
565 570 575
Phe Pro Gly Lys His Ala Leu Ala Met Ser Ser Ala Val Tyr Lys Asn
580 585 590
Trp Asn Phe Thr Glu Gln Gly Leu Pro Asp Asp Leu Ile Lys Arg Gly
595 600 605
Ile Ala Ile Lys Asp Pro Ser Ser Pro Ser Lys Val Lys Leu Leu Ile
610 615 620
Lys Asp Tyr Pro Tyr Ala Thr Asp Gly Leu Ala Ile Trp Gln Ala Ile
625 630 635 640
Glu Gln Trp Val Thr Glu Tyr Cys Ala Ile Tyr Tyr Pro Asn Asp Gly
645 650 655
Val Leu Gln Gly Asp Val Glu Leu Gln Ala Trp Trp Lys Glu Val Arg
660 665 670
Glu Val Gly His Gly Asp Leu Lys Asp Ala Asp Trp Trp Pro Lys Met
675 680 685
Gln Ser Leu Pro Glu Leu Thr Lys Ala Cys Thr Thr Ile Ile Trp Ile
690 695 700
Ala Ser Ala Leu His Ala Ala Val Asn Phe Gly Gln Tyr Pro Tyr Ala
705 710 715 720
Gly Tyr Leu Pro Asn Arg Pro Thr Ile Ser Arg Arg Pro Met Pro Glu
725 730 735
Pro Gly Ser Lys Glu Tyr Thr Glu Leu Asp Glu Asn Pro Glu Lys Phe
740 745 750
Phe Ile Arg Thr Ile Thr Ser Gln Phe Gln Thr Ile Leu Gly Val Ser
755 760 765
Leu Ile Glu Ile Leu Ser Lys His Ser Ala Asp Glu Ile Tyr Leu Gly
770 775 780
Gln Arg Asp Thr Pro Glu Trp Thr Ser Asp Pro Lys Ala Leu Glu Ala
785 790 795 800
Phe Lys Arg Phe Ser Arg Gln Leu Val Glu Ile Glu Ser Lys Val Leu
805 810 815
Asn Met Asn Lys Asp Pro Leu Leu Lys Asn Arg Val Gly Pro Ala Asn
820 825 830
Phe Pro Tyr Thr Leu Met Phe Pro Asn Thr Ser Asp Asn Lys Gly Ala
835 840 845
Ala Glu Gly Ile Thr Ala Arg Gly Ile Pro Asn Ser Ile Ser Ile
850 855 860
<210> 6
<211> 619
<212> PRT
<213> 稻瘟病菌（Magnaporthe oryzae）
<400> 6
Met Arg Val Leu Val Trp Ile Ala Gly Leu Ala Pro Leu Ala Val Ala
1 5 1015
Val Pro Ser Ser Ser Tyr Arg Val Ala Val Ala Ala Arg Ala Asp Asn
202530
Thr Ser Ala Ser Val Ala Pro Ser Gln Asn Val Ser Gly Ala Ala Pro
354045
Pro Glu Leu Val Val Tyr Thr Leu Pro Cys Glu Asp Gly Asn Ser Thr
505560
Ala Arg Thr Ala Glu Ile Arg Leu Lys Gln Ala Thr Leu Leu Tyr Gly
65707580
Pro Ser Leu Leu Gly Asn Ala Ser Tyr Phe Pro Gly Gly Pro Leu Gly
859095
Asp Ala Ile Ser Leu Arg Asp Gln Thr Val Trp Glu Gly Ala Ala Val
100 105 110
Val Gln Ser Leu Arg Ala Phe Thr Asp Ala Ala Lys Val Ala Ala Asn
115 120 125
Ile Lys Gln Asn Gly Gly Leu Asn Ser Leu Asp Asp Phe Lys Val Leu
130 135 140
Tyr Gln Asp Gly Trp Lys Gly Ser Val Pro Gln Gly Ile Ala Arg Gly
145 150 155 160
Gln Ser Glu Asn Tyr Thr Ser Asp Leu Leu Phe Ser Met Glu Arg Leu
165 170 175
Ser Val Asn Pro Tyr Ile Leu Lys Arg Leu His Pro Thr Glu Asp Ala
180 185 190
Leu Pro Phe Gln Val Asp Arg Ala Thr Val Lys Gln Leu Thr Lys Thr
195 200 205
Ser Leu Lys Ala Leu His Ala Ala Gly Arg Leu Phe Val Ala Asp His
210 215 220
Ser Tyr Gln Arg Asn Tyr Thr Arg Leu Ala Asn Arg Tyr Ser Ala Ala
225 230 235 240
Cys Thr Ala Leu Phe Tyr Leu Asp Pro Arg Ser Asn Gln Phe Leu Pro
245 250 255
Leu Ala Ile Lys Thr Asn Val Gly Ala Asp Leu Thr Tyr Thr Pro Leu
260 265 270
Asp Thr Asp Asn Asn Asn Trp Leu Leu Ala Lys Ile Met Phe Asn Asn
275 280 285
Asn Asp Leu Phe His Gly Gln Ile Phe His Val Ala Tyr Pro His Ala
290 295 300
Ile Ala Glu Ile Val His Leu Ala Ala Leu Arg Thr Met Ser Ala Arg
305 310 315 320
His Pro Val Leu Ala Leu Met Glu Arg Leu Met Tyr Gln Ala Tyr Ala
325 330 335
Val Arg Pro Leu Gly Glu Arg Val Leu Phe Asn Lys Gly Gly Leu Phe
340 345 350
Glu Gln Asn Phe Ala Tyr Pro Gln Asp Met Val Tyr Lys Phe Val Gly
355 360 365
Asp Ser Tyr Pro Thr Thr Gly Arg Trp Arg Ala Gly Tyr Leu Asp Thr
370 375 380
Asp Val Arg Ala Arg Gly Leu Val Asp Ala Asp Tyr Gly Pro Glu Leu
385 390 395 400
Pro His Phe Pro Phe Tyr Glu Asp Gly Ser Arg Leu Val Glu Val Ile
405 410 415
Arg Arg Phe Val Arg Ser Phe Val Asp Ala Thr Tyr His Glu Ser Asp
420 425 430
Glu Met Val Ala Lys Asp Ala Glu Leu Gln Ala Trp Val Ala Glu Ala
435 440 445
Asn Gly Pro Ala Gly Val Glu Asp Phe Glu Pro Gly Pro Leu Asp Thr
450 455 460
Arg Glu Arg Leu Val Glu Val Leu Thr His Met Ala Trp Leu Thr Gly
465 470 475 480
Cys Ala His His Val Leu Asn Gln Gly Glu Pro Val Thr Ala Ser Gly
485 490 495
Val Leu Pro Met His Pro Thr Ala Leu Tyr Ala Pro Val Pro Thr Ser
500 505 510
Lys Ala Asn Thr Thr Ala Asp Leu Leu Gly Tyr Leu Pro Ser Ala Gln
515 520 525
Lys Ser Val Asp Gln Val Thr Leu Leu Ala Arg Phe Asn Arg Pro Asp
530 535 540
Val Val Pro Thr Asn Gln Thr Leu Arg Tyr Met Phe Ala Ala Pro Gln
545 550 555 560
Leu Leu Leu Gly Asn Gly Glu Ala Tyr Arg Arg Ala Asn Gln Arg Phe
565 570 575
Val Arg Ala Met Gly Arg Ile Ser Asp Glu Val Lys Ala Arg Arg Phe
580 585 590
Asp Asp Arg Gly Leu Ser Gln Gly Met Pro Phe Ile Trp Gln Ala Leu
595 600 605
Asp Pro Gly Asn Ile Pro Phe Tyr Leu Ser Val
610 615
<210> 7
<211> 1121
<212> PRT
<213> 烟曲霉（Aspergillus fumigatus）
<400> 7
Met Leu Arg Arg Phe Ser Ser Thr Phe Lys Lys Lys Gly Asp Arg Glu
1 5 1015
Ser Lys Gln Asn Gly Thr Ala Ser Ser Ser Ser Ala Ala Val Ala Asn
202530
Thr Asn Asn Asn Asp Asn Lys Arg His Ser Lys Ile Ser Ala Ala Arg
354045
Lys Ser Ser Ser Asp Asp Asp Arg Asn Glu Lys Lys Gly Asn Ser Val
505560
Ser Pro Phe Glu Lys Tyr Ala Ser Val Leu His Ala Ser Arg Ser Pro
65707580
Ile Pro Asn Gln Thr Gly Asp Gly Ala Tyr Leu Glu His Glu His Thr
859095
Thr Ser Leu Leu Gln Asp Ala Arg His Leu Gly Phe Lys Asp Phe Lys
100 105 110
Thr Leu Lys Glu Val Ile Glu Ser Lys Leu Pro Gly Gly Gln Leu Ile
115 120 125
Asp Asp Lys Thr Met Leu Met Glu Arg Ile Ile Gln Leu Val Ser Arg
130 135 140
Leu Pro His Asn Ser Lys His Arg Glu Glu Leu Thr Asn Ala Phe Leu
145 150 155 160
Thr Glu Leu Trp Asp Ser Leu Pro His Pro Pro Leu Ser Tyr Met Gly
165 170 175
Asn Asp Tyr Ala Tyr Arg Ser Ala Asp Gly Ser Asn Asn Asn Pro Thr
180 185 190
Leu Pro Arg Leu Gly Ala Ala Asn Thr Leu Tyr Ala Arg Thr Ile Pro
195 200 205
Pro Leu Ile Ile Gln Pro Gly Gly Leu Pro Asp Pro Gly Leu Val Phe
210 215 220
Asp Thr Leu Phe Ala Arg Gln Thr Phe Lys Pro His Pro Asn Lys Val
225 230 235 240
Ser Ser Val Phe Phe Tyr Trp Ala Ser Leu Ile Ile His Asp Ile Phe
245 250 255
Gln Thr Asp Tyr Lys Asn Pro Asn Met Asn Lys Thr Ser Gly Tyr Leu
260 265 270
Asp Leu Ser Ile Leu Tyr Gly Asp Val Gln Glu Glu Gln Asn Leu Ile
275 280 285
Arg Thr Phe Lys Asp Gly Lys Leu Lys Pro Asp Ser Phe Ser Glu Pro
290 295 300
Arg Leu Gln Ala Phe Pro Ala Thr Cys Cys Val Leu Met Val Met Leu
305 310 315 320
Asn Arg Phe His Asn Tyr Ala Val Glu Gln Leu Ala Ala Ile Asn Glu
325 330 335
Asn Gly Arg Phe Thr Lys Pro Ala Asp Asn Leu Ser Glu Glu Glu Ala
340 345 350
Lys Lys Ala Trp Ala Lys Tyr Asp Glu Asp Leu Phe Gln Thr Gly Arg
355 360 365
Leu Ile Thr Cys Gly Leu Tyr Ile Asn Ile Thr Leu Tyr Asp Tyr Leu
370 375 380
Arg Thr Ile Val Asn Leu Asn Arg Thr Asn Ser Thr Trp Cys Leu Asp
385 390 395 400
Pro Arg Ala Gln Met Glu Gly Ser His Thr Ala Pro Ser Gly Leu Gly
405 410 415
Asn Gln Cys Ser Val Glu Phe Asn Leu Ala Tyr Arg Trp His Ser Ala
420 425 430
Thr Ser Ala Thr Asp Glu Lys Trp Thr Glu Asp Val Tyr Glu Arg Leu
435 440 445
Met Gly Lys Pro Ala Ser Glu Val Ser Met Thr Glu Leu Leu Met Gly
450 455 460
Leu Gly Lys Tyr Gln Ala Glu Leu Pro Lys Asp Pro Ser Lys Arg Thr
465 470 475 480
Phe Ala Asp Leu Glu Arg Gln Ala Asp Gly Arg Phe Lys Asp Glu Asp
485 490 495
Leu Val Asn Leu Leu Val Asn Ala Val Glu Asp Val Ala Gly Ser Phe
500 505 510
Gly Ala Arg Asn Val Pro Lys Val Leu Lys Asn Val Glu Ile Leu Gly
515 520 525
Ile Ile Gln Ser Arg Lys Trp Asn Val Gly Ser Leu Asn Glu Phe Arg
530 535 540
Lys Phe Phe Gly Leu Lys Pro Tyr Glu Thr Phe Glu Glu Ile Asn Ser
545 550 555 560
Asp Pro Asp Val Ala Glu Ser Leu Arg Ser Leu Tyr Asp His Pro Asp
565 570 575
Phe Val Glu Leu Tyr Pro Gly Ile Val Ala Glu Glu Ala Lys Gln Pro
580 585 590
Met Val Pro Gly Val Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Ala
595 600 605
Val Leu Ser Asp Ala Val Ala Leu Val Arg Gly Asp Arg Phe Tyr Thr
610 615 620
Ile Asp Tyr Asn Pro Arg Asn Leu Thr Asn Trp Gly Tyr Ser Glu Val
625 630 635 640
Arg Tyr Asp Leu Ser Ile Asn Gln Gly Cys Ile Phe Tyr Lys Leu Ala
645 650 655
Thr Arg Ala Phe Pro Asn Trp Phe Lys Pro Asp Ser Ile Tyr Ala His
660 665 670
Tyr Pro Met Thr Ile Pro Ser Glu Asn Arg Lys Ile Met Lys Asp Leu
675 680 685
Gly Arg Glu Ile His Tyr Ser Trp Asp Arg Pro Gln Tyr Thr Pro Pro
690 695 700
Arg Val Asp Leu Val Ser Tyr Ser Asn Ala Lys Leu Val Ala Glu Gln
705 710 715 720
Gln Asn Gln Phe Arg Ala Ala Trp Gly Asp Thr Val Glu Phe Val Phe
725 730 735
Gly Lys Ala Ser Lys Glu Phe Lys Leu Tyr Gln Asp Ser Ala Phe Ile
740 745 750
Gln Lys His Ala Asp Val Met Ser Lys Leu Leu Asn Lys Glu Glu Trp
755 760 765
His Arg Ser Val Lys Glu Phe Tyr Glu Asp Ile Thr Ala Lys Leu Leu
770 775 780
Glu Asp Lys Thr Arg Arg Phe Gly Gly Ile Asn Gln Val Asp Ile Thr
785 790 795 800
Asn Asp Val Gly Asn Leu Thr Pro Val Ile Phe Ala Ala Asn Val Phe
805 810 815
Ser Leu Pro Leu Lys Ser Lys Glu Asn Pro Arg Gly Ile Tyr Thr Glu
820 825 830
His Glu Met Phe Lys Val Leu Ala Ala Leu Tyr Asn Cys Leu Tyr Phe
835 840 845
Asp Ile Asp Lys Thr Lys Ser Tyr Pro Leu His His Ala Ser Gln Ala
850 855 860
Val Gly Glu Pro Leu Gly Lys Ala Leu Glu Ala Asn Val Lys Ala Leu
865 870 875 880
Gly Gly Ser Ser Leu Leu Ser Gly Ile Phe Arg Ser Phe Arg Glu Asn
885 890 895
Lys Asn Ala Leu Lys Glu Tyr Gly Val His Leu Thr Lys Gln Leu Leu
900 905 910
Glu Asn Gly Leu Gly Ala His Glu Ile Ala Trp Ala Gln Phe Leu Pro
915 920 925
Thr Val Ile Ala Met Val Pro Ala Gln Ala Gln Ala Phe Thr Gln Ile
930 935 940
Val Asp Phe Tyr Leu Ser Lys Glu Gly Ser Lys His Leu Pro Ala Ile
945 950 955 960
Gln Arg Leu Ala Lys Gln Asp Thr Lys Lys Ser Asp Glu Gln Leu Leu
965 970 975
His Tyr Cys Leu Glu Ala Val Arg Leu Asn Asp Met Ser Gly Leu Tyr
980 985 990
Arg Gln Ser Glu Thr Thr Leu Ala Val Thr Asp Glu Ala Val Glu Val
995 10001005
Thr Ile Gln Pro Gly Asp Lys Val Phe Val Ser Phe Ala Lys Ala
101010151020
Asn Arg Asp Ala Ser Val Phe Pro Asp Pro Ala Glu Val Arg Leu
102510301035
Asp Arg Pro Met Asn Ser Tyr Ile Asn Pro Thr Leu Gly Pro His
104010451050
Gly Phe Leu Ser Lys Glu Thr Ser His Ile Ala Leu Thr Ala Met
105510601065
Leu Arg Ala Val Gly Arg Leu Asn Asn Leu Arg Val Ala Pro Gly
107010751080
Val Gln Gly Gln Leu Lys Lys Ile Pro Gln Pro Gly Gly Tyr Ser
108510901095
Ala Tyr Leu Arg Glu Asp His Gly Ser Tyr Ser Ile Phe Pro Thr
110011051110
Thr Phe Arg Val Gln Tyr Asp Ala
11151120
<210> 8
<211> 1117
<212> PRT
<213> 构巢曲霉（Aspergillus nidulans）
<400> 8
Met Leu Arg Arg Phe Ser Thr Phe Arg Lys Ser Lys Gly Asp Lys Thr
1 5 1015
Glu Lys Ala Asp Arg Asp Ser Lys Ala Asn Gly Ser Asn Ala Asn Ser
202530
Ala Ala Ala Ala Ser Asn Ser Ser Lys Arg Gln Ser Lys Val Pro Pro
354045
Pro Arg Arg Pro Ser Ser Asp Ser Gly Ser Ser Ala Glu Ser Glu Asp
505560
Val Pro Ala Val Phe Glu Lys Tyr Ala Gln Val Leu His Ala Ser Ser
65707580
Arg Pro Ile Pro His Gln Gly Gly Glu Ala Ala Tyr Leu Glu Lys Glu
859095
His Pro Ser Gly Leu Phe Asn Asp Leu Lys Ser Leu Gly Phe Lys Asp
100 105 110
Phe Ala Ser Leu Lys Asp Val Ile Lys Thr Lys Ile Asn Gly Glu Leu
115 120 125
Thr Asp Asp Lys Thr Met Ile Met Glu Arg Ile Ile Gln Ile Val Ser
130 135 140
Ser Leu Pro Ser Asn Ser Lys Met Arg Val Asp Leu Thr Asn Met Phe
145 150 155 160
Leu Asp Glu Leu Trp Gly Ser Leu Pro His Pro Pro Leu Ser Tyr Met
165 170 175
Gly Asn Asp Tyr Gln Tyr Arg Ser Ala Asp Gly Ser Asn Asn Asn Pro
180 185 190
Thr Leu Pro Trp Leu Gly Ala Ala Asn Thr Ala Tyr Ala Arg Ser Ile
195 200 205
Glu Pro Leu Thr Val Gln Pro Gly Gly Leu Pro Asp Ala Gly Leu Val
210 215 220
Phe Asp Thr Leu Phe Ala Arg Gln Lys Phe Thr Pro His Pro Asn Lys
225 230 235 240
Val Ser Ser Leu Phe Phe Asp Trp Ala Ser Leu Ile Ile His Asp Ile
245 250 255
Phe Gln Thr Asp Tyr Arg Asp Tyr Asn Lys Asn Lys Thr Ser Ala Tyr
260 265 270
Leu Asp Leu Ala Ile Leu Tyr Gly Asp Val Gln Glu Glu Gln Asp Leu
275 280 285
Val Arg Thr His Lys Asp Gly Lys Leu Lys Pro Asp Ser Phe Ser Glu
290 295 300
Pro Arg Leu Gln Ala Phe Pro Ala Ala Cys Cys Val Leu Leu Val Met
305 310 315 320
Leu Asn Arg Phe His Asn Tyr Val Val Glu Glu Leu Ala Ala Ile Asn
325 330 335
Glu Asn Gly Arg Phe Thr Lys Pro Ser Pro Asp Leu Pro Glu Glu Gln
340 345 350
Ala Lys Lys Ala Trp Ala Lys Tyr Asp Glu Asp Leu Phe Gln Thr Gly
355 360 365
Arg Leu Ile Thr Cys Gly Leu Phe Ile Asn Ile Thr Leu Tyr Asp Tyr
370 375 380
Leu Arg Thr Ile Val Asn Leu Asn Arg Val Asn Ser Thr Trp Cys Leu
385 390 395 400
Asp Pro Arg Ala Gln Met Glu Gly Ser Ala Thr Pro Ala Gly Leu Gly
405 410 415
Asn Gln Cys Ser Val Glu Phe Asn Leu Ala Tyr Arg Trp His Ser Ala
420 425 430
Ile Ser Ala Asn Asp Glu Lys Trp Thr Glu Lys Val Tyr Glu Glu Leu
435 440 445
Ile Gly Lys Pro Gly Ser Glu Ile Ser Thr Gln Glu Leu Leu Met Gly
450 455 460
Leu Gly Lys Tyr Gly Ala Ser Leu Pro Lys Asp Pro Ser Gln Arg Thr
465 470 475 480
Phe Ala Gly Leu Lys Arg Gln Glu Asp Gly Thr Phe Lys Asp Glu Glu
485 490 495
Leu Val Asn Ile Leu Thr Ser Ala Ile Glu Asp Val Ala Gly Ser Phe
500 505 510
Gly Ala Arg Asn Val Pro Lys Val Leu Lys Ala Val Glu Val Leu Gly
515 520 525
Ile Glu Gln Gly Arg Lys Trp Asn Val Gly Ser Leu Asn Glu Phe Arg
530 535 540
Lys Phe Phe Gly Leu Lys Asn Tyr Glu Thr Phe Glu Glu Ile Asn Ser
545 550 555 560
Asp Pro Glu Val Ala Glu Ser Leu Arg Ala Leu Tyr Gly His Pro Asp
565 570 575
Tyr Val Glu Leu Tyr Pro Gly Ile Val Ser Glu Glu Ala Lys Glu Pro
580 585 590
Met Ile Pro Gly Val Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Ala
595 600 605
Val Leu Ser Asp Ala Val Ala Leu Val Arg Gly Asp Arg His Tyr Thr
610 615 620
Val Asp Tyr Asn Pro Arg Asn Leu Thr Asn Trp Gly Tyr Asn Glu Val
625 630 635 640
Arg Tyr Asp Leu Asn Ile Asn Gln Gly Cys Val Phe Tyr Lys Leu Ala
645 650 655
Thr Arg Ala Phe Pro Asn Trp Phe Lys Pro Asp Ser Ile Tyr Ala His
660 665 670
Tyr Pro Met Thr Ile Pro Ser Glu Asn Lys Val Ile Met Lys Asn Leu
675 680 685
Gly Arg Glu Ala Asp Tyr Ser Trp Asp Arg Pro Gln Tyr Gln Ala Pro
690 695 700
Arg Ala Ser Leu Thr Ser Tyr Ser Asn Val Lys Leu Ile Leu Asp Gln
705 710 715 720
Gln Lys Asp Phe Arg Val Val Trp Gly Asp Cys Thr Pro Leu His Ser
725 730 735
Gly Lys Gly Gly Glu Asp Phe Trp Ser Lys Thr Leu Ser Asp Pro Gln
740 745 750
Phe Lys Lys Ser Ile Lys Glu Phe Tyr Glu Lys Thr Thr Leu Glu Leu
755 760 765
Phe Ala Asp Lys Ser Val Asn Leu Ala Gly Arg Lys Gln Ile Asp Ile
770 775 780
Val Lys Asp Val Gly Asn Ile Val Pro Ala Arg Phe Ala Ser Lys Leu
785 790 795 800
Leu Ser Leu Pro Leu Arg Ser Lys Glu Asn Ser Lys Gly Val Phe Thr
805 810 815
Asp His Glu Ile Phe Met Ala Leu Ala Val Ile Tyr Asn Ala Ile Phe
820 825 830
Phe Asp Val Asp Thr Thr Lys Ser Phe Pro Leu Arg Lys Ala Ala Asp
835 840 845
Ala Val Ser Lys Glu Leu Gly Lys His Val Glu Ser His Val Lys Ser
850 855 860
Val Ser Ser Pro Gly Phe Leu Ser Arg Val Ile Asp Asn Phe Arg Asp
865 870 875 880
Asp His Asn Ala Leu Lys Asp Leu Gly Asp Gln Leu Ile Lys Arg Leu
885 890 895
Ala Glu Gly Gly Leu Ser Val Ser Asp Ile Thr Tyr Gly Gln Ile Leu
900 905 910
Pro Thr Ala Val Glu Leu Val His Gly Gln Ala Gln Met Phe Thr Arg
915 920 925
Val Val Glu Tyr Tyr Leu Asn Glu Gly Lys Gln His Leu Pro Glu Leu
930 935 940
Ser Leu Leu Ala Lys Gln Asp Ser Ala Glu Thr Asp Ala Lys Leu Thr
945 950 955 960
Arg Tyr Ala Leu Glu Ala Ile Arg Leu Asn Ala Gly Ser Gly Ala Tyr
965 970 975
Arg Lys Ala Glu Thr Asn Phe Tyr Phe Lys Glu Gly Asp Ala Asp Ile
980 985 990
Asn Leu Lys Pro Gly Asp Glu Ile Phe Ile Ser Ser Thr Gln Ala Asn
995 10001005
Arg Asp Pro Thr Ala Phe Pro Asp Pro Asp Glu Val Arg Leu Asp
101010151020
Arg Pro Asp Glu Ser Tyr Leu Asn Tyr Gly Ile Gly Ser Gln Ile
102510301035
Gly Leu Gly Lys Asp Ala Thr Leu Thr Ala Val Thr Ala Met Val
104010451050
Arg Ala Ala Phe Ser Leu Glu Gly Leu Arg Pro Ala Pro Gly Val
105510601065
Gln Gly Val Leu Lys Lys Val Val Arg Pro Glu Gly Tyr Thr Leu
107010751080
Tyr Met Arg Glu Asp His Gly Ala Phe Ser Pro Phe Pro Thr Thr
108510901095
Phe Arg Val His Phe Asp Gly Glu Val Val Thr Pro Lys Lys Gln
110011051110
Ile Asp Ser Ala
1115
<210> 9
<211> 1113
<212> PRT
<213> 土曲霉（Aspergillus terreus）
<400> 9
Met Leu Arg Arg Phe Ser Thr Gln Phe Lys Lys Ser Lys Gly Asp Arg
1 5 1015
Glu Ser Lys Gln Asn Gly Thr Pro Gly Pro Ala Asn Asn Ser Ser Lys
202530
Arg Gln Ser Lys Leu Ala Gln Pro Arg Lys Ser Ser Ser Ser Ser Ser
354045
Asp Gly Glu Arg Ser Ser Lys Asn Glu Asp Gly Val Pro Ala Phe Glu
505560
Lys Tyr Ala Gln Val Leu His Ala Ser Arg Ser Pro Leu Pro Asn Gln
65707580
Thr Gly Asp Gly Ala Thr Ser Ala His Asp His Gln Thr Thr Leu Phe
859095
Gln Asp Leu Arg Ser Phe Gly Phe Lys Asp Phe Gly Thr Leu Lys Glu
100 105 110
Val Ile Ala Thr Lys Ala Lys Gly Glu His Val Asp Asp Lys Thr Met
115 120 125
Val Met Glu Arg Ile Ile Gln Leu Val Ser Gly Leu Pro Ala Asn Ser
130 135 140
Lys Ser Arg Thr Glu Leu Thr His Leu Phe Leu Asp Gln Leu Trp Asp
145 150 155 160
Ser Leu Pro His Pro Pro Leu Ser Tyr Met Gly Ser Asp Tyr Ala Tyr
165 170 175
Arg Ser Ala Asp Gly Ser Asn Asn Asn Pro Thr Leu Pro Trp Leu Gly
180 185 190
Ala Ala Asn Thr Pro Tyr Ala Arg Ser Ile Ala Pro Leu Thr Ile Gln
195 200 205
Pro Gly Gly Leu Pro Asp Ala Gly Leu Val Phe Asp Ser Leu Phe Ala
210 215 220
Arg Asp Lys Phe Arg Pro His Pro Asn Lys Val Ser Ser Val Phe Phe
225 230 235 240
Asp Trp Ala Ser Leu Ile Ile His Asp Ile Phe Gln Thr Asp His Gln
245 250 255
Asn Pro Asn Ile Asn Lys Thr Ser Gly Tyr Leu Asp Leu Ser Ile Leu
260 265 270
Tyr Gly Asp Val Lys Glu Glu Gln Asp Leu Val Arg Thr His Lys Asp
275 280 285
Gly Lys Leu Lys Pro Asp Ala Phe Ser Glu Pro Arg Leu Gln Ala Phe
290 295 300
Pro Ala Thr Cys Cys Val Leu Leu Val Met Leu Asn Arg Phe His Asn
305 310 315 320
His Val Val Glu Gln Leu Ala Glu Ile Asn Glu Asn Gly Arg Phe Thr
325 330 335
Lys Pro Ser Pro Asp Leu Pro Glu Asp Lys Ala Lys Ala Ala Trp Glu
340 345 350
Lys Tyr Asp Glu Asp Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys Gly
355 360 365
Leu Tyr Ile Asn Ile Thr Leu Tyr Asp Tyr Leu Arg Thr Ile Val Asn
370 375 380
Leu Asn Arg Val Asn Ser Thr Trp Cys Leu Asp Pro Arg Ala Gln Met
385 390 395 400
Glu Gly Ser Ser Ser Thr Pro Ser Gly Leu Gly Asn Gln Cys Ser Val
405 410 415
Glu Phe Asn Leu Ala Tyr Arg Trp His Ser Ala Ile Ser Ala Asn Asp
420 425 430
Glu Lys Trp Thr Glu Gln Val Tyr Gln Asp Leu Met Gly Lys Pro Ala
435 440 445
Glu Glu Val Ser Val Glu Glu Leu Leu Gly Gly Leu Met Lys Tyr Gly
450 455 460
Arg Ser Leu Glu Lys Asp Pro Ser Lys Arg Thr Phe Ala Gly Leu Gln
465 470 475 480
Arg Gln Ala Asp Gly Thr Phe Lys Asp Glu Glu Leu Val Glu Ile Leu
485 490 495
Thr Asn Ala Val Glu Asp Val Ala Gly Ser Phe Gly Ala Arg His Val
500 505 510
Pro Lys Ala Leu Lys Ala Val Glu Val Leu Gly Ile Asn Gln Ala Arg
515 520 525
Gln Trp Asn Val Gly Ser Leu Asn Glu Phe Arg Lys Phe Phe Asp Leu
530 535 540
Lys Pro Tyr Glu Ser Phe Glu Glu Ile Asn Ser Asp Pro Asp Val Ala
545 550 555 560
Asp Ala Leu Arg Asn Leu Tyr Glu His Pro Asp Tyr Val Glu Leu Tyr
565 570 575
Pro Gly Ile Val Ala Glu Glu Ala Lys Glu Pro Met Ile Pro Gly Val
580 585 590
Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Ala Val Leu Ser Asp Ala
595 600 605
Val Ala Leu Val Arg Gly Asp Arg His Tyr Thr Ile Asp Tyr Asn Pro
610 615 620
Arg Asn Leu Thr Asn Trp Gly Tyr Asn Glu Cys Arg Tyr Asp Leu Ser
625 630 635 640
Ile Asn Gln Gly Cys Val Phe Tyr Lys Leu Ala Thr Arg Ala Phe Pro
645 650 655
Asn Trp Phe Lys Pro Asp Ser Ile Tyr Ala His Tyr Pro Met Thr Ile
660 665 670
Pro Ser Glu Asn Arg Asn Ile Met Lys Asn Leu Gly Arg Glu Ser His
675 680 685
Tyr Ser Trp Glu Arg Pro Lys Phe Thr Pro Pro Gln Val Asn Leu Val
690 695 700
Ser Tyr Pro Asn Val Lys Leu Ala Leu Glu Gln Glu Lys Gln Leu Arg
705 710 715 720
Val Ile Trp Ser Gly Asn Thr Pro Leu Arg Pro Ala Lys Gly Gly Asp
725 730 735
Asp Phe Trp Ser Lys Ala Leu Asn Asn Asp Glu Trp Arg Lys Asn Ile
740 745 750
Lys Glu Phe Tyr Glu Asp Met Thr Ile Lys Leu Leu Asn Glu Lys Ser
755 760 765
Cys Lys Leu Gly Gly Ile Arg Gln Ile Asp Ile Thr Arg Asp Leu Gly
770 775 780
Asn Leu Ala Pro Val His Phe Ala Ser Lys Val Phe Ser Leu Pro Leu
785 790 795 800
Lys Thr Lys Gln Asn Ser Lys Gly Val Phe Thr Glu His Glu Met Phe
805 810 815
Met Ile Met Ala Val Val Phe Thr Ser Val Phe Phe Asp Val Asp Pro
820 825 830
Thr Lys Ser Phe Pro Leu His Phe Ala Ser Arg Ala Val Ser Gln Gln
835 840 845
Leu Gly Ser Ala Ile Glu Ser His Val Lys Ser Ile Gly His Pro Gly
850 855 860
Phe Leu Ser Ala Ile Ile Asp Ser Phe Arg Asp Asp Asp Asn Val Leu
865 870 875 880
Lys Glu Tyr Gly Asp Gln Leu Ile Lys Lys Leu Leu Asp Ser Gly Leu
885 890 895
Gly Val Ser Asp Val Thr Tyr Ser Gln Ile Leu Pro Thr Ala Val Ser
900 905 910
Met Val His Asn Gln Ala Arg Met Phe Thr His Ile Val Asp Tyr Tyr
915 920 925
Val Thr Glu Gly Lys Lys His Leu Pro Glu Ile Asn Arg Leu Ala Lys
930 935 940
Glu Ser Thr Pro Glu Ala Asp Glu Lys Leu Thr Arg Tyr Cys Leu Glu
945 950 955 960
Ala Phe Arg Leu Phe Gly Thr Phe Gly Ser Tyr Arg Glu Ala Gln Thr
965 970 975
Asp Phe Thr Val Asn Asp Glu Ser Gly Pro Val Asp Ile Lys Gln Gly
980 985 990
Asp Lys Val Phe Val Gly Ala Val Lys Ala Asn Arg Asp Pro Ser Val
995 10001005
Phe Pro Asp Pro Asp Glu Val Arg Leu Asp Arg Pro Leu Asp Ser
101010151020
Tyr Ile Gln Phe Gly Leu Gly Pro His Ala Gly Leu Gly Lys Glu
102510301035
Ala Thr Leu Leu Ala Leu Thr Ala Met Leu Arg Val Val Gly Arg
104010451050
Leu Asp Asn Leu Arg Pro Ala Pro Gly Pro Gln Gly Gln Leu Lys
105510601065
Lys Ile Pro Arg Glu Gly Gly Tyr Tyr Val Tyr Leu Arg Glu Asp
107010751080
Trp Gly Ser Tyr Ser Pro Phe Pro Thr Thr Phe Lys Val His Phe
108510901095
Asp Gly Glu Leu Pro Ala Pro Lys Lys Arg Gly Ile Ala Ser Ala
110011051110
<210> 10
<211> 1117
<212> PRT
<213> 棒曲霉（Aspergillus clavatus）
<400> 10
Met Leu Arg Arg Phe Ser Ser Thr Phe Asn Lys Lys Lys Gly Asp Arg
1 5 1015
Glu Pro Lys Gln Asn Gly Val Ala Thr Thr Thr Thr Thr Thr Thr Pro
202530
Thr Thr Asn Lys Arg Tyr Ser Lys Val Pro Glu Gly His Lys Ser Ser
354045
Ser Glu Glu Glu Arg Asn Glu Lys Lys Gly Gly Ala Val Ser Pro Phe
505560
Glu Lys Tyr Ala Ser Val Leu His Ala Ser Arg Thr Pro Ile Pro Asn
65707580
Gln Thr Gly Asp Gly Ala Tyr Leu Glu His Glu His Thr Thr Ser Leu
859095
Leu Gln Asp Ala Arg His Leu Gly Phe Lys Asp Leu Ala Thr Leu Lys
100 105 110
Glu Val Ile Lys Asn Lys Ala Thr Gly Gln Leu Val Asp Asp Lys Thr
115 120 125
Met Leu Met Glu Arg Val Ile Gln Leu Val Ser Ser Leu Pro His Asn
130 135 140
Ser Lys His Arg Glu Glu Leu Thr His Ser Phe Leu Asp Glu Leu Trp
145 150 155 160
Gly Ser Leu Pro His Pro Pro Leu Ser Tyr Met Gly Ser Glu Tyr Ala
165 170 175
Tyr Arg Ser Ala Asp Gly Ser Asn Asn Asn Pro Thr Leu Pro Trp Leu
180 185 190
Gly Ala Ala Asn Thr Ala Tyr Ala Arg Thr Ile Ala Pro Leu Ile Ile
195 200 205
Gln Pro Gly Gly Leu Pro Asp Pro Gly Leu Val Phe Asp Thr Leu Phe
210 215 220
Ala Arg Gln Ser Phe Lys Pro His Pro Asn Asn Val Ser Ser Leu Phe
225 230 235 240
Phe Asp Trp Ala Ser Leu Ile Ile His Asp Ile Phe Gln Thr Asp Tyr
245 250 255
Arg Asn Pro His Val Asn Lys Thr Ser Gly Tyr Leu Asp Leu Ser Ile
260 265 270
Leu Tyr Gly Asp Val Gln Glu Glu Gln Asn Leu Ile Arg Thr Phe Glu
275 280 285
Gly Gly Arg Leu Lys Thr Asp Ser Phe Ser Glu Pro Arg Leu Gln Ala
290 295 300
Phe Pro Ala Ala Cys Cys Val Leu Leu Val Met Leu Asn Arg Phe His
305 310 315 320
Asn His Val Val Glu Gln Leu Ala Ala Ile Asn Glu Asn Gly Arg Phe
325 330 335
Thr Gln Pro Arg Asp Gly Leu Pro Glu Asp Gln Ala Lys Lys Ala Trp
340 345 350
Glu Lys Tyr Asp Glu Asp Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys
355 360 365
Gly Leu Tyr Ile Asn Ile Thr Leu Tyr Asp Tyr Leu Arg Thr Ile Val
370 375 380
Asn Leu Asn Arg Thr Asn Ser Thr Trp Cys Leu Asp Pro Arg Ala Gln
385 390 395 400
Met Glu Gly Asn Asn Thr Thr Pro Ser Gly Leu Gly Asn Gln Cys Ser
405 410 415
Val Glu Phe Asn Leu Ala Tyr Arg Trp His Ser Ala Ile Ser Ala Asn
420 425 430
Asp Glu Lys Trp Thr Glu Lys Ile Tyr Glu Asp Leu Met Gly Lys Pro
435 440 445
Ala Ser Glu Val Ser Met Thr Glu Leu Leu Met Gly Leu Gly Lys Tyr
450 455 460
Glu Ala Gly Leu Ser Lys Asp Pro Ser Gln Arg Thr Phe Ala Gly Leu
465 470 475 480
Glu Arg Gln Ala Asp Gly Arg Phe Arg Asp Glu Asp Leu Val Asn Ile
485 490 495
Leu Thr Gly Ala Ile Glu Asp Val Ala Gly Ser Phe Gly Ala Arg Asn
500 505 510
Val Pro Lys Val Leu Lys Asn Val Glu Val Leu Gly Ile Leu Gln Ser
515 520 525
Arg Lys Trp Asn Val Gly Ser Leu Asn Glu Phe Arg Lys Phe Phe Gly
530 535 540
Leu Lys Pro Tyr Glu Thr Phe Glu Glu Ile Asn Ser Asp Pro Asp Val
545 550 555 560
Ala Glu Ser Leu Arg Ser Leu Tyr Asp His Pro Asp Phe Val Glu Leu
565 570 575
Tyr Pro Gly Ile Val Ser Glu Glu Ala Lys Glu Pro Met Ile Pro Gly
580 585 590
Val Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Ala Val Leu Ser Asp
595 600 605
Ala Val Ala Leu Val Arg Gly Asp Arg Tyr Tyr Thr Ile Asp Tyr Asn
610 615 620
Ala Arg Asn Leu Thr Asn Trp Gly Tyr Ser Glu Val Arg Tyr Asp Leu
625 630 635 640
Ser Ile Asn Gln Gly Cys Val Phe Tyr Lys Leu Ala Thr Arg Ala Phe
645 650 655
Pro Asp Trp Phe Lys Ser Asp Ser Ile Tyr Ala His Tyr Pro Met Thr
660 665 670
Ile Pro Ser Glu Asn Arg Lys Ile Met Lys Asn Leu Gly Arg Glu Ala
675 680 685
His Tyr Ser Tyr Asp Arg Pro Gln Tyr Ile Pro Pro Pro Val Asp Leu
690 695 700
Leu Ser Tyr Pro Ser Ala Lys Leu Val Ala Glu Gln Arg Lys Asp Phe
705 710 715 720
His Thr Val Trp Ala Asp Thr Val Glu Phe Val Phe Gly Lys Ala Ser
725 730 735
Lys Asn Phe Lys Leu Ser Glu Asp Thr Ala Phe Ile Glu Arg Gln Arg
740 745 750
Glu Thr Ile Ser Lys Leu Leu Ser Gln Asp Glu Trp Gln Arg Asn Val
755 760 765
Lys Glu Phe Tyr Glu Glu Ile Thr Thr Gly Leu Leu Glu Glu Lys Thr
770 775 780
Arg Arg Phe Ala Gly Ile Asn Gln Val Asp Ile Thr Arg Asp Ile Gly
785 790 795 800
Asn Leu Val Pro Val Ile Phe Ala Ser Asn Leu Phe Ser Leu Pro Leu
805 810 815
Arg Ser Lys Glu Asn Ser Arg Gly Ile Phe Thr Glu His Glu Met Phe
820 825 830
Asn Val Leu Ala Val Leu Tyr Asn Cys Ile Tyr Phe Asp Ile Asp Lys
835 840 845
Thr Lys Ser Phe Pro Leu His His Ala Ser Gln Ala Val Gly Glu Gln
850 855 860
Leu Gly Lys Ala Val Glu Ala Asn Val Lys Ser Leu Gly Gly Ser Ser
865 870 875 880
Leu Leu Ser Gly Ile Leu Gly Gly Ser Arg Asp Asn Lys Asn Ala Leu
885 890 895
Lys Glu Tyr Gly Ala His Met Thr Lys Gln Leu Leu Glu Asn Gly Leu
900 905 910
Gly Ala Ser Glu Ile Thr Trp Ser Gln Ile Leu Pro Thr Val Ile Ala
915 920 925
Met Val Pro Ser Gln Ala Gln Ala Phe Thr Gln Ile Ile Asp Phe Tyr
930 935 940
Leu Ser Lys Glu Gly Ser Lys Tyr Leu Pro Glu Ile Gln Arg Leu Ala
945 950 955 960
Arg Glu Glu Ser Lys Glu Ser Asp Glu Gln Leu Leu Arg Tyr Cys Met
965 970 975
Glu Ala Ile Arg Leu Asn Lys Thr Ser Gly Ala Tyr Arg Glu Ala Arg
980 985 990
Thr Ser Leu Thr Val Thr Glu Glu Thr Gly Gln Val Thr Leu Gln Pro
995 10001005
Gly Asp Lys Val Phe Val Gly Phe Ala Lys Ala Asn Arg Asp Pro
101010151020
Ser Val Phe Pro Asp Pro Ser Glu Val Arg Leu Asp Arg Pro Leu
102510301035
Asp Ala Tyr Ile Asn His Ser Leu Gly Pro His Gly Phe Leu Ser
104010451050
Lys Glu Thr Ser Gln Ile Ala Leu Thr Ala Met Leu Arg Ala Val
105510601065
Gly Arg Leu Asn Asn Leu Arg Arg Ala Pro Gly Ala Gln Gly Glu
107010751080
Val Lys Lys Val Pro Ile Ala Asp Gly Tyr Ser Ala Tyr Leu Arg
108510901095
Glu Asp His Gly Ser Tyr Ser Val Phe Pro Thr Thr Phe Arg Val
110011051110
His Tyr Asp Val
1115
<210> 11
<211> 1125
<212> PRT
<213> 马尼弗氏青霉（Penicillium marneffei）
<400> 11
Met Arg Arg Phe Ser Ser Val Phe Lys Lys Lys Glu Glu Ser Lys Ala
1 5 1015
Lys Glu Asn Gly His Ile Ser Glu Lys Val Asn Gly Lys Arg Gln Ser
202530
Lys Ala Val Ser Thr Ser Gln Pro Pro Ala Glu Pro Glu Asp His Ser
354045
Lys Ala Arg His Glu Val Ser Ala Ile Phe Glu Arg Tyr Ala Gln Val
505560
Ile His Ala Ser Arg Gln Pro Leu Pro Thr Gln Ser Gly Asp Gly Thr
65707580
Tyr Leu Glu His Gly His Asp His Ser Ser Ser Leu Phe Ser Asp Leu
859095
Arg Ser Leu Gly Phe Lys Asp Tyr Gly Thr Leu Val Glu Val Met Lys
100 105 110
Asn Lys Ala Ser Gly Ser Tyr Val Asp Asp Lys Thr Met Leu Met Glu
115 120 125
Arg Ile Ile Gln Leu Val Ser Gly Leu Pro Ser Asn Ser Glu Arg Arg
130 135 140
Thr Glu Leu Thr Asn Ala Phe Leu Asp Glu Leu Trp Asp Ser Leu Pro
145 150 155 160
His Pro Pro Leu Ser Phe Val Gly Pro Lys Phe Glu Tyr Arg Ser Ala
165 170 175
Asp Gly Ser Trp Asn Asn Pro Thr Ile Pro Trp Leu Gly Ala Ala Asn
180 185 190
Thr Glu Tyr Ser Arg Ser Ile Pro Pro Leu Thr Ile Gln Pro Ser Gly
195 200 205
Leu Pro Asp Ala Gly Leu Val Phe Asp Ser Ile Met Ala Arg Glu Lys
210 215 220
Phe Thr Pro His Pro Asn Lys Val Ser Ser Val Phe Phe Ala Trp Ala
225 230 235 240
Ser Leu Ile Ile His Asp Ile Phe Gln Thr Asp Tyr Arg Asn Pro His
245 250 255
Ile Ser Gln Thr Ser Ser Tyr Leu Asp Leu Ser Ile Leu Tyr Gly Asp
260 265 270
Asn Gln Asp Asp Gln Asn Gln Met Arg Thr Phe Lys Asp Gly Lys Ile
275 280 285
Lys Pro Asp Ser Phe Ser Glu Pro Arg Leu Gln Ala Phe Pro Ala Met
290 295 300
Cys Asn Val Leu Met Val Met Leu Asn Arg Phe His Asn Asn Val Val
305 310 315 320
Glu Gln Ile Ala Gln Ile Asn Glu Asn Gly Arg Phe Asn Lys Pro Arg
325 330 335
Pro Gly Leu Ser Pro Glu Gln Thr Glu Ala Ala Trp Lys Lys Tyr Asp
340 345 350
Glu Asp Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys Gly Leu Tyr Ile
355 360 365
Asn Ile Thr Leu Tyr Asp Tyr Leu Arg Thr Ile Val Asn Leu Asn Arg
370 375 380
Thr Asn Ser Thr Trp Cys Leu Asp Pro Arg Ala Arg Met Gln Gly Thr
385 390 395 400
His Thr Thr Pro Ser Gly Leu Gly Asn Gln Cys Ser Val Glu Phe Asn
405 410 415
Leu Ala Tyr Arg Trp His Ser Ala Thr Ser Tyr Asn Asp Glu Lys Trp
420 425 430
Thr Glu Glu Val Tyr Arg Asp Leu Phe Gly Lys Pro Ala Glu Glu Ile
435 440 445
Ser Met Pro Glu Leu Leu Met Gly Leu His Lys Tyr Glu Gln Ser Ile
450 455 460
Asp Lys Asp Pro Ser Lys Arg Thr Phe Ala Asn Leu Gln Arg Gln Ala
465 470 475 480
Asp Gly Thr Phe Lys Asp Asp Asp Leu Val Lys Ile Met Thr Ser Ala
485 490 495
Val Glu Asp Val Ala Gly Ser Phe Gly Ala Arg Asn Val Pro Lys Val
500 505 510
Met Arg Ser Ile Glu Ile Leu Gly Ile Glu Gln Ala Arg Lys Trp Asn
515 520 525
Val Gly Ser Leu Asn Glu Phe Arg Lys Phe Phe Asn Leu Lys Pro Tyr
530 535 540
Glu Thr Phe Glu Glu Ile Asn Ser Asp Pro Tyr Val Ala Asp Gln Leu
545 550 555 560
Arg His Leu Tyr Glu His Pro Asp Tyr Val Glu Leu Tyr Pro Gly Ile
565 570 575
Val Ala Glu Glu Ala Lys Ala Pro Met Val Pro Gly Val Gly Ile Ala
580 585 590
Pro Thr Tyr Thr Ile Ser Arg Ala Val Leu Ser Asp Ala Val Ala Leu
595 600 605
Val Arg Gly Asp Arg Phe Tyr Thr Thr Asp Gln Thr Pro Arg Asn Leu
610 615 620
Thr Asn Trp Gly Trp Gln Glu Ser Gly His Asp Leu Asn Ile Asn Gln
625 630 635 640
Gly Cys Val Phe Tyr Lys Leu Ala Phe Arg Ala Phe Pro Asn His Phe
645 650 655
Lys Pro Asp Ser Ile Tyr Ala His Tyr Pro Met Thr Ile Pro Glu Glu
660 665 670
Asn Lys Val Ile Met Lys Asp Leu Gly Arg Glu Asp Asp Tyr Ser Trp
675 680 685
Asp Gln Pro Thr Phe Asn Ala Pro Arg Val Glu Leu Lys Thr Tyr Gln
690 695 700
Ala Ala Gln Thr Val Leu Asn Asp Thr Arg Asn Phe Arg Val Thr Trp
705 710 715 720
Gly Asp Ala Ser Ala Trp Val Phe Gly Glu Lys Thr Gly Phe Asp Tyr
725 730 735
Met Leu Ser Gly Asp Thr Pro Phe His Gly Gln Gln Arg Glu Leu Leu
740 745 750
Glu Arg Ser Leu Tyr Gln Glu Gly Trp Asp Ser Arg Leu Lys Glu Phe
755 760 765
Tyr Glu Gln Ile Thr Leu Gln Leu Leu His Asp Lys Ser Tyr Thr Leu
770 775 780
Ala Gly Thr Lys Gln Val Asp Leu Thr Arg Asp Val Gly Asn Leu Ala
785 790 795 800
Thr Val His Phe Ala Ala His Leu Phe Gly Leu Pro Leu Lys Thr Lys
805 810 815
Gly Asn Pro Arg Gly Ile Phe Thr Glu His Glu Leu Tyr Met Ala Asn
820 825 830
Ala Val Ile Phe Gln Ala Val Phe Phe Asp Tyr Asp Leu Met Arg Ser
835 840 845
Tyr Pro Leu Arg Gln Ala Ala Arg Ala Val Ala Lys Lys Ile Gly Glu
850 855 860
Met Val Glu Leu Asn Val Lys Ser Ile Gly Ser Asn Gly Ile Ile Ser
865 870 875 880
His Phe Ile Asp Gly Leu Arg Lys Gln Asp Asn Pro Leu Ser Asp Tyr
885 890 895
Gly Glu Asn Leu Val Lys Lys Leu Leu Asp Ser Gly Leu Asn Ala His
900 905 910
Glu Val Thr Trp Thr Gln Ile Leu Pro Ala Val Val Ser Met Val Pro
915 920 925
Lys Leu Gly Gln Val Phe Thr Gln Ile Ile Asp Phe Tyr Leu Ser Asp
930 935 940
Ala Gly Lys Ala Tyr Leu Pro Glu Ile Asn Arg Leu Thr Lys Leu Asn
945 950 955 960
Thr Pro Glu Ser Asp Gln Ala Leu Thr His Tyr Val Leu Glu Ala Ile
965 970 975
Arg Leu Asn Gly Thr Tyr Gly Ala Tyr Arg Glu Ala Gln Arg Asp Val
980 985 990
Thr Val Asn Asp Gly Gly Asn Glu Ile Gln Val Asn Lys Gly Ala Lys
995 10001005
Val Phe Val Ser Phe Ile Ser Ala Ser Arg Asp Pro Ala Ala Phe
101010151020
Pro Glu Pro Glu Lys Val Val Leu Asp Arg Pro Val Glu Ser Tyr
102510301035
Val His Tyr Gly Ile Gly Pro His Ser Cys Leu Gly Gln Asp Ala
104010451050
Ser Leu Ile Ala Leu Thr Ser Met Leu Arg Val Val Gly Arg Leu
105510601065
Glu Asn Leu Arg Arg Ala Arg Gly Thr Gln Gly Leu Leu Lys Lys
107010751080
Ile Pro Arg Glu Gly Gly Ser Tyr His Tyr Leu Arg Glu Asp Gly
108510901095
Gly Ser Phe Thr Pro Phe Pro Ala Thr Phe Lys Val Glu Trp Asp
110011051110
Ser Glu Leu Pro Pro Leu Lys Asn Arg Gly Thr Trp
111511201125
<210> 12
<211> 1117
<212> PRT
<213> 斜卧青霉（Penicillium decumbens）
<400> 12
Met Leu Arg Arg Phe Ser Thr Gln Phe Arg Lys Asn Lys Glu Ser Asn
1 5 1015
Ser Asp Ala Asp Ser Ser Lys Asn Ser Lys Glu Asn Gly Lys Arg His
202530
Ser Lys Pro Ile Gln Pro Ser Arg Lys Ala Ser Ser His Asp Glu Lys
354045
Gln His Ser Val Asn Arg Ala Glu Val Val Ala Val Phe Glu Lys His
505560
Ala Gln Ala Ile His Ala Ser Arg Glu Pro Leu Pro Ser Gln Gly Gly
65707580
Asp Gly Ala Tyr Leu Lys His Asp Gln Ser Ser Gly Leu Phe Glu Asp
859095
Ile Lys Ser Leu Gly Phe Arg Asp Leu Ser Thr Val Lys Ser Leu Ile
100 105 110
Lys Ser Lys Ala Ser Gly Glu Leu Ile Asp Asp Lys Thr Tyr Leu Met
115 120 125
Glu Arg Ile Ile Gln Leu Val Ala Asp Met Pro Gly His Ser Lys Asn
130 135 140
Arg Val Glu Leu Thr Asn Gln Phe Leu Asp Glu Leu Trp Asp Ser Leu
145 150 155 160
Pro His Pro Pro Leu Ser Tyr Met Gly Asp Glu Tyr Lys Tyr Arg Ser
165 170 175
Ala Asp Gly Ser Arg Asn Asn Pro Thr Leu Pro Gln Leu Gly Ala Ala
180 185 190
Asn Thr Pro Tyr Cys Arg Thr Ile Pro Pro Leu Thr Ile Gln Pro Ser
195 200 205
Gly Leu Pro Asp Ala Gly Leu Leu Phe Asp Ser Leu Phe Ala Arg Gln
210 215 220
Lys Phe Thr Pro His Pro Asn Lys Val Ser Ser Ile Phe Phe Asp Trp
225 230 235 240
Ala Ser Leu Ile Ile His Asp Ile Phe Gln Thr Asp Tyr Arg Gln Gln
245 250 255
His Val Asn Lys Thr Ser Ser Tyr Leu Asp Leu Ser Ile Leu Tyr Gly
260 265 270
Asp Val Lys Glu Gln Gln Asp Leu Ile Arg Ser His Gln Asp Gly Lys
275 280 285
Leu Lys Pro Asp Cys Phe Ser Glu Gly Arg Leu Gln Ala Leu Pro Ala
290 295 300
Ala Cys Gly Val Leu Leu Val Met Leu Asn Arg Phe His Asn His Val
305 310 315 320
Val Glu Gln Leu Ala Ala Ile Asn Glu Asn Gly Arg Phe Thr Lys Pro
325 330 335
Leu Asp Gly Leu Pro Glu Asp Ala Ala Lys Lys Ala Trp Ala Lys Tyr
340 345 350
Asp Glu Asp Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys Gly Leu Tyr
355 360 365
Ile Asn Ile Thr Leu Tyr Asp Tyr Leu Arg Thr Ile Val Asn Leu Asn
370 375 380
Arg Thr Asn Ser Thr Trp Cys Leu Asp Pro Arg Ala Gln Met Glu Lys
385 390 395 400
Thr Gly Ala Thr Pro Ser Gly Leu Gly Asn Gln Cys Ser Val Glu Phe
405 410 415
Asn Leu Ala Tyr Arg Trp His Ser Thr Ile Ala Gln Gly Asp Glu Lys
420 425 430
Trp Ile Glu Gln Ile Tyr Tyr Asp Leu Met Gly Lys Pro Ala Glu Glu
435 440 445
Val Ser Met His Glu Leu Leu Met Gly Met Lys Lys Val Glu Gly Ser
450 455 460
Leu Asp Pro Asp Pro Ser Lys Arg Thr Phe Ala Arg Leu Glu Arg Gln
465 470 475 480
Ala Asp Gly Thr Phe Lys Asp Glu Glu Leu Val Ala Ile Leu Thr Ser
485 490 495
Ala Cys Glu Asp Val Ala Ser Ser Phe Gly Pro Arg Asn Val Pro Lys
500 505 510
Ala Leu Arg Ser Ile Glu Ile Leu Gly Ile Glu Ala Ala Arg Arg Trp
515 520 525
His Val Gly Ser Leu Asn Glu Phe Arg Lys His Phe Gly Leu Lys Pro
530 535 540
Tyr Asp Thr Phe Glu Glu Ile Asn Ser Asn Pro Glu Ile Ala Asn Cys
545 550 555 560
Leu Arg His Leu Tyr Glu His Pro Asp Tyr Val Glu Leu Tyr Pro Gly
565 570 575
Ile Val Ser Glu Glu Pro Lys Glu Pro Met Val Pro Gly Val Gly Ile
580 585 590
Ala Pro Thr Tyr Thr Ile Ser Arg Ala Ile Leu Ser Asp Ala Val Ala
595 600 605
Leu Val Arg Gly Asp Arg His Tyr Thr Ile Asp Tyr Asn Ala Arg Asn
610 615 620
Leu Thr Asn Trp Gly Tyr Asn Glu Cys Arg Tyr Asp Leu Asn Ile Asn
625 630 635 640
Gln Gly Cys Val Phe Tyr Lys Leu Ala Leu Arg Ala Phe Pro Asn Trp
645 650 655
Tyr Lys Pro Asp Ser Ile Tyr Ala His Tyr Pro Met Thr Ile Pro Ser
660 665 670
Glu Asn Arg Asn Ile Met Lys Ser Leu Gly Arg Glu Gln Asp Tyr Ser
675 680 685
Trp Glu Arg Pro Ser Phe Thr Pro Ser Arg Ile Asn Leu Ser Ser His
690 695 700
Asn Asn Ala Arg Leu Val Leu Glu Asn Ser Thr Asp Phe Thr Pro Val
705 710 715 720
Trp Ser Glu Ala Met Thr Glu Leu Phe Gly Lys Gly Glu Phe Gly Ala
725 730 735
Gln Gln Arg Glu Ala Met Ser Ala Ala Phe Ala Thr Glu Asp Phe Leu
740 745 750
Thr Leu Val Lys Asn Phe Tyr Glu Glu Val Thr Leu Arg Leu Ile Lys
755 760 765
Glu Lys Gly Ala Asn Leu Val Gly Thr Asn Gln Val Asp Ile Thr Arg
770 775 780
Asp Val Gly Asn Leu Ala His Val His Phe Ala Ser Ala Leu Phe Gly
785 790 795 800
Leu Pro Leu Lys Thr Asp Glu Asn Pro Arg Gly Leu Phe Thr Glu His
805 810 815
Glu Met Tyr Met Ile Leu Ala Thr Ile Tyr Ser Ala Leu Phe Tyr Asp
820 825 830
Val Asp Pro Ala Lys Ser Leu Pro Leu Asn Ser Ala Ala Ser Ala Val
835 840 845
Ala Arg Gln Leu Gly Ser Val Val Glu Ala Thr Val Lys Thr Asp Thr
850 855 860
His Ser Gly Leu Phe Ser Gly Leu Ile Asn Ser Phe Arg Pro His Asp
865 870 875 880
Asn Ala Ile Arg Glu His Gly Thr Ala Ala Leu Arg Arg Val Glu Glu
885 890 895
Ser Gly Gln Ser Ala Ser Gln Ile Thr Trp Ser His Ile Ile Pro Thr
900 905 910
Val Val Gly Met Val Pro Ser Gln Gly Gln Val Phe Thr Gln Val Ile
915 920 925
Glu Tyr Tyr Thr Ser Pro Glu Gly Lys His His Trp Ser Glu Ile Ser
930 935 940
Arg Leu Ala Arg Gln Asp Ser Lys Glu Ser Asp Glu Met Leu His Arg
945 950 955 960
Tyr Cys Leu Glu Ala Ile Arg Ile Asn Gly Thr Phe Gly Glu Tyr Arg
965 970 975
Glu Ala Lys Asn Pro Val Ile Leu Glu Glu Asn Glu Glu Val Ile Asn
980 985 990
Val Gln Pro Gly Asn Lys Ile Phe Ala Ser Phe Ile Gln Ala Asn Leu
995 10001005
Asp Pro Ser Val Phe Pro Glu Pro Asn Ser Val Asn Leu Ser Arg
101010151020
Pro Leu Ser Ser Tyr Ile His Gln Gly Gln Gly Pro Ala Asn Gly
102510301035
Leu Gly Gln Glu Thr Thr Lys Ile Ala Leu Val Ser Met Leu Arg
104010451050
Val Val Ala Arg Leu Pro Asn Leu Arg Arg Ala Pro Gly Ala Gln
105510601065
Gly Gln Leu Lys Arg Ile Pro Gln Lys Gly Gly His Tyr Val Tyr
107010751080
Leu Arg Gln Asp Gly Thr Ser Tyr Phe Pro Phe Pro Thr Thr Leu
108510901095
Lys Leu His Trp Asp Gly Asp Phe Glu Phe Gln Thr Ser Gln Thr
110011051110
Ser Lys Lys His
1115
<210> 13
<211> 1118
<212> PRT
<213> 产黄青霉（Penicillium chrysogenum）
<400> 13
Met Leu Lys Arg Phe Ser Thr Gln Phe Lys Arg Ser Lys Asp Ser Lys
1 5 1015
Asp Pro Lys Glu Ser Asn Gly Asp Ala Glu Pro Lys Ser Thr Asp Lys
202530
Ser Ser Lys Arg Ala Ser Lys Val Ser Pro Ser Pro Lys Ser Thr His
354045
Lys Glu Glu Asn His Val Val Lys Arg Ser Glu Val Val Ala Val Phe
505560
Asp Lys Tyr Ala Gln Ala Ile His Ala Ser Gln Glu Pro Leu Pro Asn
65707580
Gln Thr Ser Asp Gly Ala Tyr Leu Lys His Asp Lys Ser Ser Gly Leu
859095
Ile Asn Asp Ile Lys Ala Leu Gly Phe Arg Asp Val Gly Thr Val Lys
100 105 110
Asp Leu Ile Ala Ser Lys Ala Ser Gly Gly Ile Ile Asp Asp Lys Thr
115 120 125
Tyr Leu Met Glu Arg Ile Ile Gln Met Val Ala Asp Leu Pro Gly Asn
130 135 140
Ser Lys Asn Arg Thr Asp Leu Thr Gly Leu Phe Leu Asp Asp Leu Trp
145 150 155 160
Asn Ser Ile Pro His Pro Pro Leu Ser Tyr Met Gly Asp Asp Tyr Lys
165 170 175
Tyr Arg Ser Ala Asp Gly Ser Asn Asn Asn Pro Thr Leu Pro Trp Leu
180 185 190
Gly Ala Ala Asn Thr Pro Tyr Cys Arg Thr Ile Ala Pro Leu Thr Ile
195 200 205
Gln Pro Ser Gly Leu Pro Asp Ala Gly Leu Ile Phe Asp Thr Leu Tyr
210 215 220
Ala Arg Gln Glu Phe Thr Pro His Pro Asn Lys Val Ser Ser Val Phe
225 230 235 240
Phe Asp Trp Ala Ser Leu Ile Ile His Asp Ile Phe Gln Thr Asp Tyr
245 250 255
Ser Gln Gln His Leu Asn Gln Thr Ser Ala Tyr Leu Asp Leu Ser Ile
260 265 270
Leu Tyr Gly Asp Val Lys Glu Gln Gln Asp Lys Ile Arg Ser His Glu
275 280 285
Asn Gly Lys Leu Lys Pro Asp Cys Phe Ser Glu Gly Arg Leu Gln Ala
290 295 300
Leu Pro Pro Ala Cys Gly Val Leu Leu Val Met Leu Asn Arg Phe His
305 310 315 320
Asn His Ile Val Thr Gln Leu Ala Ala Ile Asn Glu Asn Gly Arg Phe
325 330 335
Ser Ala Pro Arg Pro Gly Leu Ser Glu Glu Glu Thr Lys Ala Ala Trp
340 345 350
Ala Lys Arg Asp Glu Asp Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys
355 360 365
Gly Leu Tyr Ile Asn Ile Thr Leu Tyr Asp Tyr Leu Arg Thr Ile Val
370 375 380
Asn Leu Asn Arg Thr Asn Ser Thr Trp Cys Leu Asp Pro Arg Ala Gln
385 390 395 400
Ala Glu Lys Ala Asp Ala Thr Pro Ser Gly Leu Gly Asn Gln Cys Ser
405 410 415
Val Glu Phe Asn Leu Ala Tyr Arg Trp His Ser Thr Ile Ser Gln Gly
420 425 430
Asp Glu Lys Trp Ile Glu Gln Ile Tyr Tyr Asp Leu Met Gly Lys Pro
435 440 445
Ala Glu Gln Val Ser Met Pro Glu Leu Leu Met Gly Met Lys Lys Val
450 455 460
Lys Gly Met Leu Glu Ala Asp Pro Ala Lys Arg Thr Phe Gly His Leu
465 470 475 480
Gln Arg Asn Ala Asp Gly Tyr Phe Asp Asp Gly Glu Leu Val Asn Ile
485 490 495
Leu Thr Arg Ala Thr Glu Asp Val Ala Ser Ser Phe Gly Pro Arg Asn
500 505 510
Val Pro Lys Ala Met Arg Ser Ile Glu Ile Leu Gly Ile Glu Ala Ser
515 520 525
Arg Arg Trp Asn Val Gly Ser Leu Asn Glu Phe Arg Lys His Phe Gly
530 535 540
Leu Lys Pro Tyr Glu Thr Phe Glu Asp Val Asn Ser Asn Pro Glu Ile
545 550 555 560
Ala Asn Thr Leu Arg His Leu Tyr Glu His Pro Asp Tyr Ile Glu Leu
565 570 575
Tyr Pro Gly Ile Val Ser Glu Glu Ala Lys Glu Pro Met Ile Pro Gly
580 585 590
Val Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Ala Val Leu Ser Asp
595 600 605
Ala Val Ala Leu Val Arg Gly Asp Arg His Tyr Thr Ile Asp Tyr Asn
610 615 620
Pro Arg Asn Leu Thr Asn Trp Gly Tyr Asn Glu Cys Arg Tyr Asp Leu
625 630 635 640
Asn Ile Asn Gln Gly Cys Ile Phe Tyr Lys Leu Ala Thr Arg Ala Phe
645 650 655
Pro Asn His Tyr Lys Pro Asp Ser Ile Tyr Ala His Tyr Pro Met Thr
660 665 670
Ile Pro Ser Glu Asn Arg Asn Ile Met Lys Asp Leu Gly Arg Glu Gln
675 680 685
Asp Tyr Ser Trp Asp Lys Pro Ala Phe Met Glu Pro Arg Val Asn Leu
690 695 700
Thr Ser His Gln Asn Ala Lys Leu Leu Leu Glu Asn Gln Lys Asp Phe
705 710 715 720
Arg Pro Ser Trp Ala Arg Ser Met Ser Glu Leu Phe Gly Lys Gly Glu
725 730 735
Phe Asp Thr Lys Gln Arg Glu Ala Ile Gly Lys Ala Leu Asn Thr Glu
740 745 750
Glu Phe Pro Lys Leu Val Lys Thr Phe Tyr Glu Asp Ile Thr Glu Arg
755 760 765
Leu Ile Ala Glu Lys Gly Gly Gln Leu Gly Lys Ile Asn Gln Ile Asp
770 775 780
Ile Thr Arg Asp Val Gly Asn Leu Ala His Val His Phe Ala Ser Thr
785 790 795 800
Ile Phe Gly Val Pro Leu Lys Thr Glu Asp Asn Pro Arg Gly Leu Phe
805 810 815
Thr Glu His Glu Met Tyr Met Ile Leu Ser Thr Ile Phe Ser Ala Leu
820 825 830
Phe Phe Asp Val Asp Ala Pro Arg Ser Tyr Ala Leu Asn Arg Ala Ala
835 840 845
Ser Ala Val Ser Asn Gln Leu Gly Gln Val Val Glu Ala Thr Val Lys
850 855 860
Ala Asp Thr Asn Ser Gly Leu Phe Ala Gly Ile Met Asp Asn Phe Arg
865 870 875 880
Pro His Asp Asn Ala Leu Arg Glu Phe Gly Thr Glu Ala Ile Arg Arg
885 890 895
Met Lys Glu Ala Gly Ser Ser Ala Ser Asp Ile Thr Trp Ser Ala Ile
900 905 910
Val Pro Thr Ile Val Gly Leu Val Pro Asn Gln Ala Gln Val Phe Thr
915 920 925
Gln Ile Ile Glu Phe Tyr Thr Ala Pro Glu Asn Lys Ala His Leu Ala
930 935 940
Glu Ile Asn Arg Phe Ala Lys Thr Asp Ser Ala Glu Ser Asp Glu Lys
945 950 955 960
Leu Tyr Arg Tyr Cys Leu Glu Ala Val Arg Leu Asn Gly Thr Phe Gly
965 970 975
Ala Phe Arg Glu Ala Lys Glu Thr Val Thr Val Glu Glu Asp Gly Lys
980 985 990
Thr Tyr Thr Val Gln Pro Gly Gln Gln Val Phe Ala Ser Phe Asp Gln
995 10001005
Ala Asn His Asp Pro Ser Val Phe Pro Glu Pro Asn Glu Val Asn
101010151020
Leu Asp Arg Pro Leu Asp Ser Tyr Ile Asn His Gly Gln Gly Pro
102510301035
Thr Thr Gly Phe Gly Glu Gln Val Thr Lys Ile Ala Leu Ile Ala
104010451050
Met Leu Arg Val Val Gly Arg Leu Gln Gly Leu Arg Arg Ala Pro
105510601065
Gly Ala Gln Gly Gln Leu Lys Lys Ile Pro Gln Glu Gly Gly Tyr
107010751080
Gln Val Tyr Leu Arg Gly Asp Gly Thr Ala Tyr Cys Pro Phe Pro
108510901095
Met Ser Leu Lys Leu His Trp Asp Gly Pro Phe Glu Gln Lys Lys
110011051110
Lys Val Thr Ser Ser
1115
<210> 14
<211> 1110
<212> PRT
<213> 黑曲霉（Aspergillus niger）
<400> 14
Met Leu Arg Arg Phe Ser Ser Thr Phe Lys Arg Ser Ser Lys Gly Asp
1 5 1015
Arg Asp Ser Lys Pro Asn Gly Thr Gln Val Asn Gly Gln Lys Arg Gln
202530
Ser Lys Val Pro Ala Pro Arg Lys Ser Ser Ser Asp Glu Ser His Ser
354045
Asp His Gly Val Glu Ser Asp Asp Gly Val Ser Val Phe Glu Lys Tyr
505560
Ala Gln Val Leu His Ala Ser Arg Arg Pro Leu Pro Asn Gln Asn Gly
65707580
Asp Gly Thr Tyr Leu Glu Gln Glu His Ser Gly Ser Leu Phe Lys Asp
859095
Leu Arg Ala Leu Gly Phe Lys Asp Ile Gly Thr Leu Lys Asp Leu Ile
100 105 110
Lys Thr Lys Ala Lys Gly Glu Tyr Ile Asp Asp Lys Thr Met Leu Met
115 120 125
Glu Arg Ile Ile Gln Leu Val Ser Ser Leu Pro Gly Asn Ser Lys Thr
130 135 140
Arg Val Asp Leu Thr Asn Ala Phe Leu Asp Glu Leu Trp Gly Ser Leu
145 150 155 160
Pro His Pro Pro Leu Ser Tyr Met Gly Glu Glu Tyr Ala Tyr Arg Ser
165 170 175
Ala Asp Gly Ser Asn Asn Asn Pro Thr Leu Pro Trp Leu Gly Ala Ala
180 185 190
Gly Thr Pro Tyr Ala Arg Ser Ile Ala Pro Leu Thr Ile Gln Pro Gly
195 200 205
Gly Leu Pro Asp Ala Gly Leu Val Phe Asp Cys Leu Phe Ala Arg Glu
210 215 220
Lys Phe Thr Pro His Pro Asn Lys Val Ser Ser Leu Phe Phe Asp Trp
225 230 235 240
Ala Ser Leu Val Ile His Asp Ile Phe Gln Thr Asp Tyr Thr Asn Ser
245 250 255
His Val Asn Lys Thr Ser Ala Tyr Leu Asp Leu Ser Ile Leu Tyr Gly
260 265 270
Asp Asp Gln Glu Asp Gln Asn Leu Val Arg Thr Phe Lys Asp Gly Lys
275 280 285
Leu Lys Pro Asp Thr Phe Ser Glu Gln Arg Leu Gln Ala Phe Pro Pro
290 295 300
Ala Cys Ser Val Leu Met Val Met Leu Ser Arg Phe His Asn Trp Val
305 310 315 320
Val Glu Glu Leu Ala Ala Ile Asn Glu Asn Gly Arg Phe Asn Lys Pro
325 330 335
Asp Pro Arg Leu Asp Glu Glu Lys Ala Arg Lys Ala Trp Glu Lys Tyr
340 345 350
Asp Asn Asp Leu Phe Gln Thr Gly Arg Leu Val Thr Cys Gly Leu Phe
355 360 365
Ile Asn Ile Thr Leu Tyr Asp Tyr Leu Arg Thr Ile Val Asn Leu Asn
370 375 380
Arg Ser Asn Ser Thr Trp Cys Leu Asp Pro Arg Val Gln Met Glu Gly
385 390 395 400
Thr Lys Ser Thr Pro Ser Gly Leu Gly Asn Gln Cys Ser Val Glu Phe
405 410 415
Asn Leu Ala Tyr Arg Trp His Ser Ala Ile Ser Ala Asn Asp Glu Lys
420 425 430
Trp Thr Glu Glu Ile Tyr Glu Glu Leu Met Gly Lys Pro Ala Ser Glu
435 440 445
Val Ser Met Arg Glu Leu Leu Val Gly Leu Gly Lys Tyr Glu Arg Glu
450 455 460
Ile Pro Lys Asp Pro Ser Lys Arg Thr Phe Ala Gly Leu Lys Arg Gln
465 470 475 480
Glu Asp Gly Thr Phe Lys Asp Glu Asp Leu Val Arg Ile Leu Ala Asn
485 490 495
Ala Ile Glu Asp Val Ala Ser Ser Phe Gly Ala Arg Asn Val Pro Lys
500 505 510
Val Leu Arg Ser Val Glu Ile Leu Gly Ile Glu Gln Gly Arg Lys Trp
515 520 525
Asn Val Gly Ser Leu Asn Glu Phe Arg Lys Phe Phe Asp Leu Lys Pro
530 535 540
Tyr Glu Thr Phe Glu Glu Ile Asn Ser Asp Pro Asp Val Ala Asp Ser
545 550 555 560
Leu Arg His Leu Tyr Glu His Pro Asp Tyr Val Glu Leu Tyr Pro Gly
565 570 575
Ile Val Ala Glu Glu Ala Lys Glu Pro Met Ile Pro Gly Val Gly Ile
580 585 590
Ala Pro Thr Tyr Thr Ile Ser Arg Ala Val Leu Ser Asp Ala Val Ala
595 600 605
Leu Val Arg Gly Asp Arg Tyr Tyr Thr Ile Asp Tyr Asn Pro Arg Asn
610 615 620
Leu Thr Asn Trp Gly Tyr Asn Glu Val Arg Tyr Asp Leu Asn Ile Asn
625 630 635 640
Gln Gly Cys Val Phe Tyr Lys Leu Ala Met Arg Ala Phe Pro Asn Tyr
645 650 655
Phe Lys Pro Asp Ser Ile Tyr Ala His Tyr Pro Met Thr Ile Pro Ser
660 665 670
Glu Asn Arg Asn Ile Met Lys Asn Leu Gly Arg Glu Ser His Tyr Ser
675 680 685
Tyr Asp Arg Pro Arg Tyr Thr Glu Pro Val Pro Asn Leu Leu Ser Tyr
690 695 700
Ala Asn Ala Lys Leu Val Leu Asn Asn Gln Lys Asp Phe Thr Val Pro
705 710 715 720
Trp Gly Gly Leu Ser Ser Ile His Ala Gly Lys Gly Gly Ala Asp Phe
725 730 735
Trp Ser Lys Ser Phe Asp Asn Glu Gln Trp Arg Asn Ser Val Lys Glu
740 745 750
Phe Tyr Glu Asp Ala Thr Leu Lys Leu Leu Asn Glu Lys Ser Cys Lys
755 760 765
Leu Ala Gly Asn Lys Gln Val Asp Ile Ala Arg Asp Val Gly Asn Leu
770 775 780
Val Pro Val His Phe Val Ser Lys Val Phe Ser Leu Pro Leu Lys Thr
785 790 795 800
Lys Ser Asn Pro Arg Gly Ile Phe Thr Glu His Glu Met Phe Met Ile
805 810 815
Met Ala Val Val Phe Asn Ser Thr Phe Phe Asp Val Asp Pro Thr Lys
820 825 830
Ser Phe Pro Leu Gln His Ala Ala Arg Ala Val Ser Gln Glu Leu Gly
835 840 845
Lys Val Val Glu Ala His Val Lys Ser Ile Asn His Pro Gly Phe Leu
850 855 860
His Gly Ile Ile Asp Ser Phe Arg Asp Asp His Asn Ala Leu Lys Asp
865 870 875 880
Tyr Gly Asp Gln Leu Ile Lys Arg Leu Leu Glu Ser Gly Leu Gly Val
885 890 895
Ser Asp Val Thr Trp Gly Gln Ile Leu Pro Ala Ala Val Glu Met Val
900 905 910
His Thr Gln Ser Gln Met Phe Thr Gln Ile Ile His Phe Tyr Leu Thr
915 920 925
Glu Gly Gln Lys Tyr Leu Pro Glu Ile Asn Arg Leu Ala Asn Glu Asn
930 935 940
Thr Ala Glu Ala Asn Asp Arg Leu Thr Arg Phe Cys Leu Glu Ala Val
945 950 955 960
Arg Leu Asn Gly Asn Leu Gly Ile Tyr Arg Glu Ala Gln Ala Asp Ile
965 970 975
Asn Val Ser Asp Glu Thr Gly Gln Tyr Ser Val Lys Ser Gly Glu Lys
980 985 990
Val Phe Val Gly Ser Ser Lys Ala Asn Arg Asp Pro Gln Ala Phe Pro
995 10001005
Ser Pro Asp Glu Val Arg Leu Asp Arg Pro Leu Asp Ser Tyr Leu
101010151020
His Tyr Gly Leu Gly Pro Gln Ser Gly Leu Gly Lys Asp Ala Thr
102510301035
Leu Ala Ala Val Thr Ala Met Val Arg Val Val Ala Arg Leu Asp
104010451050
Thr Leu Arg Pro Ala Pro Gly Ala Gln Gly Gln Leu Lys Lys Ile
105510601065
Pro Gln Glu Ala Gly Phe Ser Val Tyr Met Arg Glu Asp Tyr Gly
107010751080
Ser Tyr Ser Pro Phe Pro Thr Thr Tyr Lys Val His Tyr Asn Gly
108510901095
Asp Val Pro Ala Pro Lys Arg Gln Val Thr Thr Ala
110011051110
<210> 15
<211> 542
<212> PRT
<213> 点形念珠藻（Nostoc punctiforme）
<400> 15
Met Ala Gly Lys Arg Asp Thr Ser Lys Asp Gly Leu Gly Asn Lys Ile
1 5 1015
Gln Thr Leu Val Leu Thr Asn Phe Lys Gly Leu Trp Gln Leu Leu Gln
202530
Ser Asn Glu Phe Ile Lys Arg Lys Val Asn Lys Thr Leu Leu Asn Ser
354045
Leu Ile Tyr Lys Ile Pro Thr Arg Pro Asn Ala Tyr Ser Met Met Thr
505560
Leu Asp Glu His Ile Pro Asp Thr Asn Ile Pro Lys Lys Thr Asp Thr
65707580
Tyr Thr Ser Trp Glu Ser Leu Asn Asp Arg Thr Tyr Thr Gly Arg His
859095
Leu Pro Pro Asp Pro Lys Leu Asn Ala Glu Gly Asn Leu Pro Lys Val
100 105 110
Glu Asp Leu Ala Ile Leu Phe Arg Lys Arg Asp Gly Lys Thr Ile Tyr
115 120 125
Ser Gly Lys Ser Thr Met Leu Phe Pro Tyr Trp Val Gln Trp Phe Thr
130 135 140
Asp Ser Phe Leu Arg Leu Asn His Tyr Asn Lys Leu Lys Asn Thr Ser
145 150 155 160
Asn His Glu Ile Asp Leu Cys Asn Val Tyr Gly Leu Thr Arg Lys Gln
165 170 175
Thr His Leu Leu Arg Ser Phe Gln Gly Gly Lys Leu Lys Thr Gln Lys
180 185 190
Leu Lys Arg Gln Asp Gly Val Glu Glu Glu Tyr Pro Leu Phe Tyr Tyr
195 200 205
Ala Asp Pro Ala Gln Asp Lys Val Lys Pro Glu Phe Glu Gly Leu Tyr
210 215 220
Glu Pro Ile Asn Asp Glu Lys Arg Gln Pro Ile Asp Lys Lys Gln Tyr
225 230 235 240
Met Phe Ala Met Gly Val Glu Arg Ala Asn Val Gln Ile Gly Tyr Val
245 250 255
Met Leu Asn Thr Leu Cys Phe Arg Glu His Asn Arg Leu Cys Asp Glu
260 265 270
Leu Ala Arg Asn Tyr Pro Asp Trp Asp Asp Glu Arg Leu Phe Gln Thr
275 280 285
Ser Arg Asn Ile Leu Met Ala Ile Ile Leu Lys Ile Ile Met Glu Glu
290 295 300
Tyr Ile Asn His Ile Thr Pro Tyr His Phe Lys Leu Phe Ala Asp Pro
305 310 315 320
Glu Ala Phe Thr Lys Glu Ser Trp His Arg Pro Asn Tyr Met Ala Ile
325 330 335
Glu Phe Asp Phe Val Tyr Arg Trp His Ser Ala Ile Pro Glu Thr Phe
340 345 350
Asn Tyr Asn Gly Lys Pro Thr His Ile Val Thr Ser Leu Trp Asn Asn
355 360 365
Lys Met Phe Ile Asp Gln Gly Leu Gly Ala Leu Met Glu Glu Thr Cys
370 375 380
Ser Gln Pro Gly Thr Lys Ile Gly Leu Phe Asn Thr Pro Asp Ile Leu
385 390 395 400
Val Glu Leu Thr Glu Leu Pro Ser Ile Arg Leu Gly Arg Gln Leu Gln
405 410 415
Leu Ala Ser Tyr Asn Asp Tyr Arg Glu Leu Cys Gly Phe Pro Arg Val
420 425 430
Thr Arg Phe Glu Gln Val Thr Gly Asn Glu Phe Ala Gln Glu Lys Leu
435 440 445
Lys Glu Leu Tyr Gly His Val Asp Lys Ile Glu Phe Phe Val Gly Leu
450 455 460
Tyr Ala Glu Asp Gly Arg Glu Asn Ser Thr Ile Pro Ala Leu Val Ala
465 470 475 480
Arg Leu Ile Gly Ile Asp Ala Phe Ser Gln Ala Leu Thr Asn Pro Leu
485 490 495
Leu Ser Pro Asn Ile Phe Asn Lys Glu Thr Phe Ser Pro Val Gly Trp
500 505 510
Glu Ile Leu Gln Asn Thr Lys Thr Val Ser Asp Leu Val Asn Arg Asn
515 520 525
Val Pro Pro Ser Gly Lys Lys Tyr Lys Val Thr Phe Asp Leu
530 535 540
<210> 16
<211> 634
<212> PRT
<213> 铜绿假单胞菌（Pseudomonas aeruginosa）
<400> 16
Met Thr Leu Ser Arg Leu Ser Leu Ala Ile Leu Ser Val Leu Ala Gly
1 5 1015
Ala Pro Ala Phe Ala Asp Asp Ser Gly Val Asp Leu Asp Gln Gly Trp
202530
Asn Gln Thr Gln Lys Thr Ala Trp Leu Glu Ala Gly Gln Gly Ser Arg
354045
Met Leu Pro Leu Ala Trp Leu Val Ala Leu Glu Gln Arg Ala Ser Glu
505560
Glu Pro Leu Met Ser Asp Ala Leu Ile Arg Gln Tyr Gly Tyr Val Pro
65707580
His Thr Leu Gly Gly Ser Ser Val Lys Val Val Gln Gly Tyr Ala Val
859095
Asp Arg Ser Asp Asp Ser Asp Leu Thr Phe Thr Lys Leu Arg Trp Lys
100 105 110
Ala Leu Gln Gly Ser Arg Glu Pro Trp Val Gly Pro Thr Cys Ser Met
115 120 125
Cys His Thr Ser His Ile Ser Tyr Gln Gly Thr Gln Leu Thr Val Tyr
130 135 140
Gly Gly Gln Thr Met Gly Asp Leu Ala Gly Phe Gln Leu Glu Ile Leu
145 150 155 160
Gly Ala Leu Gln Ser Thr Arg Ala Asp Thr Ala Lys Phe Glu Arg Phe
165 170 175
Ala Arg Lys Val Leu Gly Ala Asp Gly Leu Val Ser Gly Tyr Asn Asp
180 185 190
Ala Asn Lys Ala Arg Leu Gln Ala Ala Leu Asp Ala Thr Ile Val Arg
195 200 205
Leu Arg Asp Gly Ser His Phe Asn Leu Pro His Asp Pro Glu Phe Gly
210 215 220
Pro Gly Arg Leu Asp Ala Ile Gly Ser Ile Phe Asn Ser Val Gly Tyr
225 230 235 240
Glu Leu His Ala Asp Glu Gln Ile Tyr Gly Ala Glu Asp Ala Pro Val
245 250 255
Ser Tyr Pro Phe Leu Trp Asn Val Pro Gln Leu Asp Arg Val Gln Trp
260 265 270
Thr Gly Phe Asn Pro Asn His Ile Asn Val Val Asp Ile Asp Asn Arg
275 280 285
Lys Phe Asp Val Gly Ala Leu Ala Arg Asn Ala Gly Glu Ala Val Gly
290 295 300
Val Phe Ala Asp Val Lys Val Leu Ser Pro Ile Gln Ser Ala Leu His
305 310 315 320
Ile Gly Tyr Pro Ser Ser Ile Asn Val Asp Asn Leu Ile Arg Ile Glu
325 330 335
Asp Gln Leu Gly Gln Leu Lys Pro Pro Ala Trp Pro Asn Gln Leu Phe
340 345 350
Gly Ala Pro Glu Pro Thr Arg Val Ala Glu Gly Arg Glu Leu Tyr Arg
355 360 365
Gln His Cys Ser Ser Cys His Thr Pro Leu Asp Arg Asn Asp Leu Arg
370 375 380
Thr Pro Val Lys Thr Val Leu Thr His Leu Gln Ala Arg Gly Glu Val
385 390 395 400
Ala Pro Ile Gly Thr Asp Pro Trp Thr Ala Cys Asn Ser Ile Ala Gln
405 410 415
Leu Lys Thr Gly Tyr Val Arg Gly Lys Pro Tyr Leu Ser Phe Val Gly
420 425 430
Thr Gly Gln Arg Gly Phe Tyr Gly Lys Gln Ala Tyr Ala Val Asp Val
435 440 445
Leu Gln Glu Val Val Val Gln Ala Leu Ala Ala Arg Gly Leu Ser Val
450 455 460
Ala Leu Gly Ala Phe Gln Thr Ala Ala Leu Gly Ile Phe Asp Gly Gln
465 470 475 480
Leu Pro Pro Leu Ile Ser Pro Val Pro Asp Ser Pro Asp Ala Asp Ser
485 490 495
Ala Glu Ala Thr Ala Ala Asp Ala Pro Gly Ala Leu Leu Leu Ala Glu
500 505 510
Asn Val Ala Ala Asp Ser Asp Lys Ala Arg Arg Leu Glu Gln Cys Leu
515 520 525
Ala Met Thr Ser Asp Leu Met Ala Tyr Lys Ala Arg Pro Leu Asn Gly
530 535 540
Ile Trp Ala Ser Pro Pro Tyr Leu His Asn Gly Ser Val Ala Thr Leu
545 550 555 560
Tyr Asp Leu Leu Leu Pro Pro Asp Leu Arg Pro Arg Thr Phe Tyr Thr
565 570 575
Gly Ser Val Glu Phe Asp Pro Val Asn Val Gly Tyr Ile Thr Asp Ala
580 585 590
Gly Gly Ala Asn Arg Phe Leu Phe Asp Ser Gly Lys Pro Gly Asn Ala
595 600 605
Asn Gly Gly His Asp Tyr Gly Asn Ala Gln Phe Asn Glu Gln Gln Arg
610 615 620
Arg Ala Leu Val Glu Tyr Met Lys Thr Leu
625 630
<210> 17
<211> 634
<212> PRT
<213> 铜绿假单胞菌（Pseudomonas aeruginosa）
<400> 17
Met Thr Leu Ser Arg Leu Ser Leu Ala Ile Leu Ser Val Leu Ala Gly
1 5 1015
Ala Pro Ala Phe Ala Asp Asp Ser Gly Val Asp Leu Asp Gln Gly Trp
202530
Asn Gln Thr Gln Lys Thr Ala Trp Leu Glu Ala Gly Gln Gly Ser Arg
354045
Met Leu Pro Leu Ala Trp Leu Val Ala Leu Glu Gln Arg Ala Ser Glu
505560
Glu Pro Leu Met Ser Asp Ala Leu Ile Arg Gln Tyr Gly Tyr Val Pro
65707580
His Thr Leu Gly Gly Ser Ser Val Lys Val Val Gln Gly Tyr Ala Val
859095
Asp Arg Ser Asp Asp Ser Asp Leu Thr Phe Thr Lys Leu Arg Trp Lys
100 105 110
Ala Leu Gln Gly Ser Arg Glu Pro Trp Val Gly Pro Thr Cys Ser Met
115 120 125
Cys His Thr Ser His Ile Ser Tyr Gln Gly Thr Gln Leu Thr Val Tyr
130 135 140
Gly Gly Gln Thr Met Gly Asp Leu Ala Gly Phe Gln Leu Glu Ile Leu
145 150 155 160
Gly Ala Leu Gln Ser Thr Arg Ala Asp Thr Ala Lys Phe Glu Arg Phe
165 170 175
Ala Arg Lys Val Leu Gly Ala Asp Gly Leu Val Ser Gly Tyr Asn Asp
180 185 190
Ala Asn Lys Ala Arg Leu Gln Ala Ala Leu Asp Ala Thr Ile Val Arg
195 200 205
Leu Arg Asp Gly Ser His Phe Asn Leu Pro His Asp Pro Glu Phe Gly
210 215 220
Pro Gly Arg Leu Asp Ala Ile Gly Ser Ile Phe Asn Ser Val Gly Tyr
225 230 235 240
Glu Leu His Ala Asp Glu Gln Ile Tyr Gly Ala Glu Asp Ala Pro Val
245 250 255
Ser Tyr Pro Phe Leu Trp Asn Val Pro Gln Leu Asp Arg Val Gln Trp
260 265 270
Thr Gly Phe Asn Pro Asn His Ile Asn Val Val Asp Ile Asp Asn Arg
275 280 285
Lys Phe Asp Val Gly Ala Leu Ala Arg Asn Ala Gly Glu Ala Val Gly
290 295 300
Val Phe Ala Asp Val Lys Val Leu Ser Pro Ile Gln Ser Ala Leu His
305 310 315 320
Ile Gly Tyr Pro Ser Ser Ile Lys Val Asp Asn Leu Ile Arg Ile Glu
325 330 335
Asp Gln Phe Gly Gln Leu Lys Pro Pro Ala Trp Pro Asn Gln Leu Phe
340 345 350
Gly Ala Pro Glu Pro Thr Arg Val Ala Glu Gly Arg Glu Leu Tyr Arg
355 360 365
Gln His Cys Ser Ser Cys His Thr Pro Leu Asp Arg Asn Asp Leu Arg
370 375 380
Thr Pro Val Lys Thr Val Leu Thr His Leu Gln Ala Arg Gly Glu Val
385 390 395 400
Ala Pro Ile Gly Thr Asp Pro Trp Thr Ala Cys Asn Ser Ile Ala Gln
405 410 415
Leu Lys Thr Gly Tyr Val Arg Gly Lys Pro Tyr Leu Ser Phe Val Gly
420 425 430
Thr Gly Gln Arg Gly Phe Tyr Gly Lys Gln Ala Tyr Ala Val Asp Val
435 440 445
Leu Gln Glu Val Val Val Gln Ala Leu Ala Ala Arg Gly Leu Ser Val
450 455 460
Ala Leu Gly Ala Phe Gln Thr Ala Ala Leu Gly Ile Phe Asp Gly Gln
465 470 475 480
Leu Pro Pro Leu Ile Ser Pro Val Pro Asp Ser Pro Asp Ala Asp Ser
485 490 495
Val Glu Ala Thr Ala Ala Asp Ala Pro Gly Ala Leu Leu Leu Ala Glu
500 505 510
Asn Val Ala Ala Asp Ser Asp Lys Ala Arg Arg Leu Glu Gln Cys Leu
515 520 525
Ala Met Thr Ser Asp Leu Met Ala Tyr Lys Ala Arg Pro Leu Asn Gly
530 535 540
Ile Trp Ala Ser Pro Pro Tyr Leu His Asn Gly Ser Val Ala Thr Leu
545 550 555 560
Tyr Asp Leu Leu Leu Pro Pro Asp Leu Arg Pro Arg Thr Phe Tyr Thr
565 570 575
Gly Ser Val Glu Phe Asp Pro Val Asn Val Gly Tyr Ile Thr Asp Ala
580 585 590
Gly Gly Ala Asn Arg Phe Leu Phe Asp Ser Gly Lys Pro Gly Asn Ala
595 600 605
Asn Gly Gly His Asp Tyr Gly Asn Ala Gln Phe Asn Glu Gln Gln Arg
610 615 620
Arg Ala Leu Val Glu Tyr Met Lys Thr Leu
625 630
<210> 18
<211> 610
<212> PRT
<213> 尖孢镰孢菌（Fusarium oxysporum）
<400> 18
Met Val Ala Leu Leu Ile Phe Leu Gly Ile Phe Thr Cys Val Glu Thr
1 5 1015
Leu Pro Leu Ser Asp Ser Pro Ser Ser Tyr Ile Pro Glu Glu Val Pro
202530
Ser Ser Gln Thr Ala Asp Ile Gly Leu Pro Pro Pro Thr Glu Phe Thr
354045
Leu Pro Asn Glu Asp Asp Glu Ile Leu Ile Arg Lys Leu Asn Ile Gln
505560
Lys Thr Arg Lys Glu Ile Leu Tyr Gly Pro Ser Leu Ile Gly Lys Thr
65707580
Ser Phe Phe Ile Ser Gly Pro Leu Gly Asp Gln Ile Ser Gln Arg Asp
859095
Gln Thr Leu Trp Ser Arg Asp Ala Ala Pro Val Val Gln Ala Val Ser
100 105 110
His Asp Ala Ala Ala Ala Leu His Asp Ile Gln Ile His Gly Gly Leu
115 120 125
Gln Asn Leu Asp Asp Tyr Lys Ile Leu Tyr Gln Gly His Trp Ser Ser
130 135 140
Ser Val Pro Gly Gly Ile Ala Lys Gly Gln Phe Ser Asn Phe Thr Ser
145 150 155 160
Asp Leu Leu Phe Ser Met Glu Arg Leu Ser Thr Asn Pro Tyr Ile Leu
165 170 175
Arg Arg Leu His Pro His Ala Asp Glu Leu Pro Phe Ala Val Asp Ser
180 185 190
Lys Ile Val Gln Lys Leu Thr Gly Ser Thr Leu Pro Ser Leu His Lys
195 200 205
Ala Gly Arg Leu Phe Leu Ala Asp His Ser Tyr Gln Lys Asp Tyr Val
210 215 220
Ala Gln Glu Gly Arg Tyr Ala Ala Ala Cys Gln Ala Leu Phe Tyr Leu
225 230 235 240
Asp Asp Arg Cys His Gln Phe Leu Pro Leu Ala Ile Lys Thr Asn Val
245 250 255
Gly Ser Asn Leu Thr Tyr Thr Pro Leu Asp Glu Pro Asn Asp Trp Leu
260 265 270
Leu Ala Lys Val Met Phe Asn Val Asn Asp Leu Phe His Gly Gln Met
275 280 285
Tyr His Leu Ala Ser Thr His Ala Val Ala Glu Ile Val His Leu Ala
290 295 300
Ala Leu Arg Thr Met Ser Ser Arg His Pro Val Leu Ala Leu Leu Gln
305 310 315 320
Arg Leu Met Tyr Gln Ala Tyr Ala Ile Arg Pro Ile Gly Asn Asn Ile
325 330 335
Leu Phe Asn Pro Gly Gly Leu Ile Asp Gln Asn Ser Val Phe Ser Asn
340 345 350
Val Ala Val Arg Lys Phe Ala Thr Asp Phe Tyr Pro Thr Val Ala Gly
355 360 365
Pro Val Arg Ser Asn Tyr Phe Glu Ala Asn Leu Arg Ser Arg Gly Leu
370 375 380
Leu Asn Ala Thr His Gly Pro Asp Leu Pro His Phe Pro Phe Tyr Glu
385 390 395 400
Asp Gly Ala Arg Ile Ile Lys Val Ile Arg Thr Phe Ile Gln Ser Phe
405 410 415
Val Lys Ser Ile Tyr Lys Ser Asp Lys Val Leu Ala Lys Asp Trp Glu
420 425 430
Leu Gln Ala Trp Ile Ala Glu Ala Asn Gly Ala Ala Glu Val Ile Asp
435 440 445
Phe Pro Pro Thr Pro Leu Lys Lys Arg Lys His Leu Val Asp Ile Leu
450 455 460
Thr His Met Ala Trp Leu Thr Gly Val Ser His His Val Leu Asn Gln
465 470 475 480
Gly Glu Pro Val Thr Thr Ser Gly Val Leu Pro Leu His Pro Gly Ser
485 490 495
Leu Tyr Ala Pro Val Pro Gly Glu Lys Gly Val Val Asp Ser Leu Leu
500 505 510
Pro Trp Leu Pro Asn Glu Gln Lys Ser Val Asp Gln Ile Ser Phe Leu
515 520 525
Ala Leu Phe Asn Arg Pro Gln Ile Val Glu Asn Asn Arg Thr Leu Arg
530 535 540
Tyr Met Phe Asn Ser Glu Ser Leu Leu Ala Gly Thr Val Arg Ala Val
545 550 555 560
Ala Ala Ala Asn Glu Arg Phe Met Glu Glu Met Gly His Ile Ser Gln
565 570 575
Glu Ile Ser Asn Arg Lys Phe Asp Asp Asp Gly Leu Ser Gln Gly Met
580 585 590
Pro Phe Ile Trp Thr Gly Met Asp Pro Gly Val Ile Pro Phe Tyr Leu
595 600 605
Ser Val
610
<210> 19
<211> 618
<212> PRT
<213> 小麦全蚀病菌（Gaeumannomyces graminis）
<400> 19
Met Arg Ser Arg Ile Leu Ala Ile Val Phe Ala Ala Arg His Val Ala
1 5 1015
Ala Leu Pro Leu Ala Ala Glu Asp Ala Ala Ala Thr Leu Ser Leu Thr
202530
Ser Ser Ala Ser Ser Thr Thr Val Leu Pro Ser Pro Thr Gln Tyr Thr
354045
Leu Pro Asn Asn Asp Pro Asn Gln Gly Ala Arg Asn Ala Ser Ile Ala
505560
Arg Lys Arg Glu Leu Phe Leu Tyr Gly Pro Ser Thr Leu Gly Gln Thr
65707580
Thr Phe Tyr Pro Thr Gly Glu Leu Gly Asn Asn Ile Ser Ala Arg Asp
859095
Val Leu Leu Trp Arg Gln Asp Ala Ala Asn Gln Thr Ala Thr Ala Tyr
100 105 110
Arg Glu Ala Asn Glu Thr Phe Ala Asp Ile Thr Ser Arg Gly Gly Phe
115 120 125
Lys Thr Leu Asp Asp Phe Ala Leu Leu Tyr Asn Gly His Trp Lys Glu
130 135 140
Ser Val Pro Glu Gly Ile Ser Lys Gly Met Leu Ser Asn Cys Thr Ser
145 150 155 160
Asp Leu Leu Phe Ser Met Glu Arg Leu Ser Ser Asn Pro Tyr Val Leu
165 170 175
Lys Arg Leu His Pro Thr Lys Asp Lys Leu Pro Phe Ser Val Glu Ser
180 185 190
Lys Val Val Lys Lys Leu Thr Ala Thr Thr Leu Glu Ala Leu His Lys
195 200 205
Gly Gly Arg Leu Phe Leu Val Asp His Ser Tyr Gln Lys Lys Tyr Thr
210 215 220
Pro Gln Pro Gly Arg Tyr Ala Ala Ala Cys Gln Gly Leu Phe Tyr Leu
225 230 235 240
Asp Ala Arg Ser Asn Gln Phe Leu Pro Leu Ala Ile Lys Thr Asn Val
245 250 255
Gly Val Asp Leu Thr Tyr Thr Pro Leu Asp Asp Lys Asp Asp Trp Leu
260 265 270
Leu Ala Lys Ile Met Phe Asn Asn Asn Asp Leu Phe Tyr Ser Gln Met
275 280 285
Tyr His Val Leu Phe His Thr Ile Pro Glu Ile Val His Glu Ala Ala
290 295 300
Phe Arg Thr Leu Ser Asp Arg His Pro Val Met Gly Val Leu Asn Arg
305 310 315 320
Leu Met Tyr Gln Ala Tyr Ala Ile Arg Pro Val Gly Gly Ala Val Leu
325 330 335
Phe Asn Pro Gly Gly Phe Trp Asp Gln Asn Phe Gly Leu Pro Ala Ser
340 345 350
Ala Ala Ile Asp Phe Pro Gly Ser Val Tyr Ala Gln Gly Gly Gly Gly
355 360 365
Phe Gln Ala Gly Tyr Leu Glu Lys Asp Leu Arg Ser Arg Gly Leu Ile
370 375 380
Gly Glu Asp Ser Gly Pro Arg Leu Pro His Phe Pro Phe Tyr Glu Asp
385 390 395 400
Ala His Arg Leu Ile Gly Ala Ile Arg Arg Phe Met Gln Ala Phe Val
405 410 415
Asp Ser Thr Tyr Gly Ala Asp Asp Gly Asp Asp Gly Ala Leu Leu Arg
420 425 430
Asp Tyr Glu Leu Gln Asn Trp Ile Ala Glu Ala Asn Gly Pro Ala Gln
435 440 445
Val Arg Asp Phe Pro Ala Ala Pro Leu Arg Arg Arg Ala Gln Leu Val
450 455 460
Asp Val Leu Thr His Val Ala Trp Ile Thr Gly Gly Ala His His Val
465 470 475 480
Met Asn Gln Gly Ser Pro Val Lys Phe Ser Gly Val Leu Pro Leu His
485 490 495
Pro Ala Ala Leu Tyr Ala Pro Ile Pro Thr Ala Lys Gly Ala Thr Gly
500 505 510
Asn Gly Thr Arg Ala Gly Leu Leu Ala Trp Leu Pro Asn Glu Arg Gln
515 520 525
Ala Val Glu Gln Val Ser Leu Leu Ala Arg Phe Asn Arg Ala Gln Val
530 535 540
Gly Asp Arg Lys Gln Thr Val Arg Asp Ala Phe Ala Ala Pro Asp Leu
545 550 555 560
Leu Ala Gly Asn Gly Pro Gly Tyr Ala Ala Ala Asn Ala Arg Phe Val
565 570 575
Glu Asp Thr Gly Arg Ile Ser Arg Glu Ile Ala Gly Arg Gly Phe Asp
580 585 590
Gly Lys Gly Leu Ser Gln Gly Met Pro Phe Val Trp Thr Ala Leu Asn
595 600 605
Pro Ala Val Asn Pro Phe Phe Leu Ser Val
610 615
<210> 20
<211> 609
<212> PRT
<213> 刺盘孢炭疽菌（Colletotrichum gloeosporioides）
<400> 20
Met Arg Leu Leu Leu Ser Ile Ala Gly Leu Thr Thr Val Val Asn Ala
1 5 1015
Leu Ala Val Arg Ala Asp Gly Asn Val Thr Ser Ser Thr Ala Val Ala
202530
Thr Pro Thr Ala Trp Thr Gly Phe Pro Val Pro Thr Glu Tyr Thr Leu
354045
Pro Gln Asp Asp His Asp Phe Gln Glu Arg Lys Glu Glu Ile Lys Leu
505560
Lys Arg Asp Thr Ile Thr Tyr Val Pro Ser Ile Ile Gly Glu Thr Ser
65707580
Leu Phe Ile Gly Gly Ser Val Gly Thr Gln Ile Val Arg Gln Glu Gln
859095
Ala Lys Trp Ile Gln Asp Leu Thr Pro Val Gln Gln Asp Ala Phe Arg
100 105 110
Glu Gly Asn Ala Ser Leu Lys Ala Ile Gln Asp His Gly Gly Leu Lys
115 120 125
Thr Leu Glu Asp Tyr Lys Ile Leu Tyr Asp Gly His Trp Ser Gly Ser
130 135 140
Val Pro Gly Gly Ile Ala Gln Gly Gln Phe Asn Asn Phe Thr Ser Asp
145 150 155 160
Leu Leu Phe Ala Met Glu Arg Leu Ser Thr Asn Pro Tyr Val Val Arg
165 170 175
Arg Leu Asn Pro Glu Ser Asp Lys Ile Pro Phe Ser Val Asp Ala Asn
180 185 190
Asn Val Thr His Leu Thr Gly Thr Thr Leu Asp Thr Leu Phe Lys Ser
195 200 205
Gly Ser Leu Phe Leu Ala Asp His Ser Tyr Gln Ala Glu Tyr Thr Ala
210 215 220
Gln Asp Gly Arg Tyr Ser Ala Ala Cys Gln Ala Leu Phe Phe Leu Asp
225 230 235 240
Gln Arg Ser Gly Gln Phe Leu Pro Leu Ala Ile Lys Thr Asn Val Gly
245 250 255
Ser Asp Leu Val Tyr Thr Pro Leu Asp Asp Pro Asn Asp Trp Leu Leu
260 265 270
Ala Lys Ile Met Tyr Asn Val Asn Asp Phe Phe His Gly Gln Ile Tyr
275 280 285
His Leu Ala Asn Ser His Ala Val Ala Glu Ile Val Asn Leu Ala Ala
290 295 300
Ile Arg Thr Leu Ser Ser Arg His Pro Val Phe Gly Leu Leu Gln Arg
305 310 315 320
Leu Met Phe Gln Ala Tyr Ala Ile Arg Ala Thr Gly Glu Ile Ala Leu
325 330 335
Phe Asn Pro Gly Gly Leu Phe Asp Gln Ser Phe Ala Phe Ser Asn Val
340 345 350
Tyr Ala Arg Lys Phe Ala Thr Asp Phe Tyr Pro Thr Val Ala Gly Pro
355 360 365
Phe Gln Ala Asn Tyr Phe Glu Glu Asp Leu Arg Ala Arg Gly Leu Leu
370 375 380
Asn Ala Ser Tyr Gly Pro Glu Leu Pro His Leu Pro Phe His Glu Asp
385 390 395 400
Gly His Lys Ile Ile Asn Ala Ile Arg Thr Phe Ile Gly Thr Phe Val
405 410 415
Asp Thr Val Tyr Glu Ser Asp Lys Val Leu Ala Glu Asp Ser Glu Leu
420 425 430
Gln Ala Trp Ile Ala Glu Ala Asn Gly Pro Ala Lys Val Ile Asn Phe
435 440 445
Pro Ser Ala Pro Leu Asn Thr Arg Lys Gln Leu Ala Glu Ile Leu Thr
450 455 460
His Met Ala Trp Leu Thr Gly Val Ser His His Val Leu Asn Gln Gly
465 470 475 480
Glu Pro Phe Thr Thr Ser Gly Val Leu Pro Leu His Pro Ala Ser Leu
485 490 495
Tyr Ala Pro Val Pro Thr Ala Lys Gly Gly Ile Lys Asp Leu Leu Pro
500 505 510
Trp Leu Pro Asn Glu Gln Lys Ser Val Glu Gln Ile Ser Leu Leu Ala
515 520 525
Arg Phe Asn Arg Pro Lys Ile Val Glu Asn Asn Glu Thr Leu Leu His
530 535 540
Met Phe Asp Val Lys Thr Leu Leu Ser Gly Thr Gly Glu Ala Val Lys
545 550 555 560
Ala Ala Asn Glu Gln Phe Met Ile Ala Met Gly Thr Ile Ser Lys Glu
565 570 575
Ile Ser Thr Arg Lys Phe Asp Asp Gln Gly Leu Ser Gln Gly Met Pro
580 585 590
Phe Ile Trp Thr Gly Met Asp Pro Gly Val Ile Pro Phe Tyr Leu Ser
595 600 605
Val
<210> 21
<211> 896
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 21
Met Tyr Cys Arg Glu Ser Leu Ser Ser Leu Gln Thr Leu Asn Val Ala
1 5 1015
Lys Ser Leu Ser Ser Leu Phe Pro Lys Gln Ser Ala Leu Ile Asn Pro
202530
Ile Ser Ala Gly Arg Arg Asn Asn Leu Pro Arg Pro Asn Leu Arg Arg
354045
Arg Cys Lys Val Thr Ala Ser Arg Ala Asn Ile Glu Gln Glu Gly Asn
505560
Thr Val Lys Glu Pro Ile Gln Asn Ile Lys Val Lys Gly Tyr Ile Thr
65707580
Ala Gln Glu Glu Phe Leu Glu Gly Ile Thr Trp Ser Arg Gly Leu Asp
859095
Asp Ile Ala Asp Ile Arg Gly Arg Ser Leu Leu Val Glu Leu Ile Ser
100 105 110
Ala Lys Thr Asp Gln Arg Ile Thr Val Glu Asp Tyr Ala Gln Arg Val
115 120 125
Trp Ala Glu Ala Pro Asp Glu Lys Tyr Glu Cys Glu Phe Glu Met Pro
130 135 140
Glu Asp Phe Gly Pro Val Gly Ala Ile Lys Ile Gln Asn Gln Tyr His
145 150 155 160
Arg Gln Leu Phe Leu Lys Gly Val Glu Leu Lys Leu Pro Gly Gly Ser
165 170 175
Ile Thr Phe Thr Cys Glu Ser Trp Val Ala Pro Lys Ser Val Asp Pro
180 185 190
Thr Lys Arg Ile Phe Phe Ser Asp Lys Ser Tyr Leu Pro Ser Gln Thr
195 200 205
Pro Glu Pro Leu Lys Lys Tyr Arg Lys Glu Glu Leu Glu Thr Leu Gln
210 215 220
Gly Lys Asn Arg Glu Glu Val Gly Glu Phe Thr Lys Phe Glu Arg Ile
225 230 235 240
Tyr Asp Tyr Asp Val Tyr Asn Asp Val Gly Asp Pro Asp Asn Asp Pro
245 250 255
Glu Leu Ala Arg Pro Val Ile Gly Gly Leu Thr His Pro Tyr Pro Arg
260 265 270
Arg Cys Lys Thr Gly Arg Lys Pro Cys Glu Thr Asp Pro Ser Ser Glu
275 280 285
Gln Arg Tyr Gly Gly Glu Phe Tyr Val Pro Arg Asp Glu Glu Phe Ser
290 295 300
Thr Ala Lys Gly Thr Ser Phe Thr Gly Lys Ala Val Leu Ala Ala Leu
305 310 315 320
Pro Ser Ile Phe Pro Gln Ile Glu Ser Val Leu Leu Ser Pro Gln Glu
325 330 335
Pro Phe Pro His Phe Lys Ala Ile Gln Asn Leu Phe Glu Glu Gly Ile
340 345 350
Gln Leu Pro Lys Asp Ala Gly Leu Leu Pro Leu Leu Pro Arg Ile Ile
355 360 365
Lys Ala Leu Gly Glu Ala Gln Asp Asp Ile Leu Gln Phe Asp Ala Pro
370 375 380
Val Leu Ile Asn Arg Asp Arg Phe Ser Trp Leu Arg Asp Asp Glu Phe
385 390 395 400
Ala Arg Gln Thr Leu Ala Gly Leu Asn Pro Tyr Ser Ile Gln Leu Val
405 410 415
Glu Glu Trp Pro Leu Ile Ser Lys Leu Asp Pro Ala Val Tyr Gly Asp
420 425 430
Pro Thr Ser Leu Ile Thr Trp Glu Ile Val Glu Arg Glu Val Lys Gly
435 440 445
Asn Met Thr Val Asp Glu Ala Leu Lys Asn Lys Arg Leu Phe Val Leu
450 455 460
Asp Tyr His Asp Leu Leu Leu Pro Tyr Val Asn Lys Val Arg Glu Leu
465 470 475 480
Asn Asn Thr Thr Leu Tyr Ala Ser Arg Thr Leu Phe Phe Leu Ser Asp
485 490 495
Asp Ser Thr Leu Arg Pro Val Ala Ile Glu Leu Thr Cys Pro Pro Asn
500 505 510
Ile Asn Lys Pro Gln Trp Lys Gln Val Phe Thr Pro Gly Tyr Asp Ala
515 520 525
Thr Ser Cys Trp Leu Trp Asn Leu Ala Lys Thr His Ala Ile Ser His
530 535 540
Asp Ala Gly Tyr His Gln Leu Ile Ser His Trp Leu Arg Thr His Ala
545 550 555 560
Cys Thr Glu Pro Tyr Ile Ile Ala Ala Asn Arg Gln Leu Ser Ala Met
565 570 575
His Pro Ile Tyr Arg Leu Leu His Pro His Phe Arg Tyr Thr Met Glu
580 585 590
Ile Asn Ala Arg Ala Arg Gln Ser Leu Val Asn Gly Gly Gly Ile Ile
595 600 605
Glu Thr Cys Phe Trp Pro Gly Lys Tyr Ala Leu Glu Leu Ser Ser Ala
610 615 620
Val Tyr Gly Lys Leu Trp Arg Phe Asp Gln Glu Gly Leu Pro Ala Asp
625 630 635 640
Leu Ile Lys Arg Gly Leu Ala Glu Glu Asp Lys Thr Ala Glu His Gly
645 650 655
Val Arg Leu Thr Ile Pro Asp Tyr Pro Phe Ala Asn Asp Gly Leu Ile
660 665 670
Leu Trp Asp Ala Ile Lys Glu Trp Val Thr Asp Tyr Val Lys His Tyr
675 680 685
Tyr Pro Asp Glu Glu Leu Ile Thr Ser Asp Glu Glu Leu Gln Gly Trp
690 695 700
Trp Ser Glu Val Arg Asn Ile Gly His Gly Asp Lys Lys Asp Glu Pro
705 710 715 720
Trp Trp Pro Val Leu Lys Thr Gln Asp Asp Leu Ile Gly Val Val Thr
725 730 735
Thr Ile Ala Trp Val Thr Ser Gly His His Ala Ala Val Asn Phe Gly
740 745 750
Gln Tyr Gly Tyr Gly Gly Tyr Phe Pro Asn Arg Pro Thr Thr Thr Arg
755 760 765
Ile Arg Met Pro Thr Glu Asp Pro Thr Asp Glu Ala Leu Lys Glu Phe
770 775 780
Tyr Glu Ser Pro Glu Lys Val Leu Leu Lys Thr Tyr Pro Ser Gln Lys
785 790 795 800
Gln Ala Thr Leu Val Met Val Thr Leu Asp Leu Leu Ser Thr His Ser
805 810 815
Pro Asp Glu Glu Tyr Ile Gly Glu Gln Gln Glu Ala Ser Trp Ala Asn
820 825 830
Glu Pro Val Ile Asn Ala Ala Phe Glu Arg Phe Lys Gly Lys Leu Gln
835 840 845
Tyr Leu Glu Gly Val Ile Asp Glu Arg Asn Val Asn Ile Thr Leu Lys
850 855 860
Asn Arg Ala Gly Ala Gly Val Val Lys Tyr Glu Leu Leu Lys Pro Thr
865 870 875 880
Ser Glu His Gly Val Thr Gly Met Gly Val Pro Tyr Ser Ile Ser Ile
885 890 895
<210> 22
<211> 941
<212> PRT
<213> 水稻(Oryza sativa)
<400> 22
Met Leu Arg Pro Gln Leu Asn Pro Ser Ser Ser His His His Thr Thr
1 5 1015
Thr Thr Ser Ser Ser Ser Ser Thr Gln Leu Tyr Phe Ala Ser Ser Ser
202530
Cys Ile Ala Ser Leu Arg Arg Pro Ser Pro Pro Ser Leu Ile Ala Gly
354045
Ala Gly Cys Arg Thr Thr Arg Arg Arg Gln Gln Gly Arg Gln Arg Val
505560
Val Val Arg Cys Ala Ser Ser Ser Ala Ala Ser Ser Ala Ser Glu Ala
65707580
Ala Arg Arg Gly Thr Gly Ser Ser Asp Met Ala Pro Ala Ala Val Val
859095
Lys Val Lys Ala Val Ala Thr Ile Lys Val Thr Val Glu Gly Leu Leu
100 105 110
Asn Ser Leu Arg Pro Ser Lys Ala Ile Asp Asn Ile Arg Asp Leu Ile
115 120 125
Gly Arg Ser Leu Phe Leu Glu Leu Val Ser Ser Glu Leu Glu Ala Lys
130 135 140
Thr Gly Lys Lys Lys Ala Thr Val His Ser Tyr Ala His Lys Val Asp
145 150 155 160
Asp Asp Asp His Gly Val Val Thr Tyr Glu Ala Asp Phe Asp Val Pro
165 170 175
Thr Gly Phe Gly Pro Ile Gly Ala Val Val Val Thr Asn Glu Leu Gly
180 185 190
Gln Glu Met Phe Leu Glu Asp Leu Asn Leu Thr Ala Gly Asp Gly Ala
195 200 205
Gly Asn Ser Thr Val Leu Pro Ile Arg Cys Asn Ser Trp Val Gln Pro
210 215 220
Lys Ser Ser Ile Asp Glu Gly Thr Pro Gly Lys Arg Ile Phe Phe Ala
225 230 235 240
Lys Ala Tyr Leu Pro Gly Gln Thr Pro Ala Gly Leu Arg Ser Tyr Arg
245 250 255
Glu Glu Asp Leu Lys Gln Lys Arg Gly Asn Gly Ala Gly Gln Arg Glu
260 265 270
Ala Asp Asp Arg Val Tyr Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn
275 280 285
Pro Asp Ser Asn Gly Asp Leu Ala Arg Pro Val Leu Gly Gly Ser Lys
290 295 300
Gln Phe Pro Tyr Pro Arg Arg Cys Arg Thr Gly Arg Pro Pro Ser Lys
305 310 315 320
Lys Asp Pro Lys Ser Glu Thr Arg Lys Gly Asn Val Tyr Val Pro Arg
325 330 335
Asp Glu Glu Phe Ser Glu Val Lys Asn Ala Gln Phe Leu Leu Lys Thr
340 345 350
Leu Gln Ser Val Leu His Ala Ala Val Pro Ala Ala Gln Ser Ala Leu
355 360 365
Ile Asp Asn Leu Ser Leu Asn Leu Pro Phe Pro Ser Phe Phe Val Ile
370 375 380
Asp Lys Leu Phe Glu Asp Gly Val Glu Leu Pro Gly Val Glu Lys Leu
385 390 395 400
Gly Phe Leu His Ser Ile Val Pro Arg Leu Leu Glu Leu Leu Arg Asp
405 410 415
Ser Pro Gly Asp Lys Ile Leu Leu Phe Asp Thr Pro Ala Asn Val Gln
420 425 430
Lys Asp Lys Phe Ala Trp Leu Arg Asp Glu Glu Phe Ala Arg Glu Thr
435 440 445
Leu Ala Gly Ile Asn Pro Tyr Ala Ile Glu Leu Val Arg Glu Phe Pro
450 455 460
Leu Lys Ser Lys Leu Asp Pro Ala Val Tyr Gly Pro Ala Glu Ser Ala
465 470 475 480
Ile Thr Ala Asp Leu Leu Glu Glu Gln Met Arg Arg Val Met Thr Val
485 490 495
Glu Glu Ala Ile Ser Gln Lys Arg Leu Phe Met Leu Asp Phe His Asp
500 505 510
Leu Phe Leu Pro Tyr Val His Lys Ile Arg Ser Leu Lys His Thr Thr
515 520 525
Met Tyr Gly Ser Arg Thr Ile Phe Phe Leu Thr Asp Asp Gly Thr Leu
530 535 540
Arg Leu Leu Ala Ile Glu Leu Thr Arg Pro Ala Ser Pro Ser Gln Pro
545 550 555 560
Gln Trp Arg Gln Val Phe Thr Pro Ser Thr Asp Thr Thr Lys Ser Trp
565 570 575
Leu Trp Arg Met Ala Lys Ala His Val Arg Ala His Asp Ala Gly His
580 585 590
His Glu Leu Ile Thr His Trp Leu Arg Thr His Cys Ala Val Glu Pro
595 600 605
Tyr Ile Ile Ala Ala Asn Arg Gln Leu Ser Glu Met His Pro Ile Tyr
610 615 620
Gln Leu Leu His Pro His Phe Arg Tyr Thr Met Arg Ile Asn Ala Leu
625 630 635 640
Ala Arg Ser Arg Leu Ile Ser Ala Ala Gly Ile Ile Glu Leu Ser Phe
645 650 655
Ser Pro Gln Lys Tyr Ser Met Glu Leu Ser Ser Val Ala Tyr Asp Lys
660 665 670
Leu Trp Arg Phe Asp Met Glu Ala Leu Pro Ala Asp Leu Val Arg Arg
675 680 685
Gly Met Ala Glu Glu Asp Pro Thr Ala Glu His Gly Leu Arg Leu Ala
690 695 700
Ile Glu Asp Tyr Pro Phe Ala Asn Asp Gly Leu Leu Ile Trp Asp Ala
705 710 715 720
Ile Lys Thr Trp Val Gln Ala Tyr Val Ala Arg Phe Tyr Pro Asp Ala
725 730 735
Asp Ser Val Ala Gly Asp Glu Glu Leu Gln Ala Phe Trp Thr Glu Val
740 745 750
Arg Thr Lys Gly His Gly Asp Lys Lys Asp Ala Pro Trp Trp Pro Lys
755 760 765
Leu Asp Ser Pro Glu Ser Leu Ala His Thr Leu Thr Thr Ile Val Trp
770 775 780
Val Ala Ala Ala His His Ala Ala Val Asn Phe Gly Gln Tyr Asp Phe
785 790 795 800
Gly Gly Tyr Phe Pro Asn Arg Pro Ser Ile Ala Arg Thr Val Met Pro
805 810 815
Val Glu Glu Pro Val Asp Gly Ala Ala Met Glu Arg Phe Leu Asp Asn
820 825 830
Pro Asp Gln Ala Leu Arg Glu Cys Phe Pro Ser Gln Val Gln Ala Thr
835 840 845
Val Val Met Ala Val Leu Asp Val Leu Ser Thr His Ser Thr Asp Glu
850 855 860
Glu Tyr Leu Gly Gly Glu Gln Thr Arg Pro Trp Asn Ser Asp Ala Ala
865 870 875 880
Val Gln Ala Ala Tyr Ala Gly Phe Thr Ala Arg Leu Lys Glu Ile Glu
885 890 895
Gly Val Ile Asp Gly Arg Asn Lys Asp Arg Lys Leu Lys Asn Arg Cys
900 905 910
Gly Ala Gly Ile Leu Pro Tyr Gln Leu Met Lys Pro Phe Ser Asp Ala
915 920 925
Gly Val Thr Gly Met Gly Ile Pro Asn Ser Thr Ser Ile
930 935 940
<210> 23
<211> 685
<212> PRT
<213> 铜绿假单胞菌（Pseudomonas aeruginosa）
<400> 23
Met Lys Arg Arg Ser Val Leu Leu Ser Gly Val Ala Leu Ser Gly Thr
1 5 1015
Ala Leu Ala Asn Asp Ser Ile Phe Phe Ser Pro Leu Lys Tyr Leu Gly
202530
Ala Glu Gln Gln Arg Ser Ile Asp Ala Ser Arg Ser Leu Leu Asp Asn
354045
Leu Ile Pro Pro Ser Leu Pro Gln Tyr Asp Asn Leu Ala Gly Lys Leu
505560
Ala Arg Arg Ala Val Leu Thr Ser Lys Lys Leu Val Tyr Val Trp Thr
65707580
Glu Asn Phe Ala Asn Val Lys Gly Val Pro Met Ala Arg Ser Val Pro
859095
Leu Gly Glu Leu Pro Asn Val Asp Trp Leu Leu Lys Thr Ala Gly Val
100 105 110
Ile Val Glu Leu Ile Val Asn Phe Val Ala Ser Leu Pro Ala Ser Ala
115 120 125
Ala Ala Gln Phe Glu Arg Ile Ala Ala Gly Leu Ser Gly Asp Leu Glu
130 135 140
Ala Ala Arg Gln Val His Glu Ala Leu Leu Glu Glu Ala Lys Asn Asp
145 150 155 160
Pro Ala Ala Ala Gly Ser Leu Leu Leu Arg Phe Thr Glu Leu Gln Thr
165 170 175
Arg Val Ile Ala Leu Leu Thr Arg Val Gly Leu Leu Val Asp Asp Ile
180 185 190
Leu Lys Ser Ala Ser Asn Leu Val Thr Gln Gly Gly Gln Gly Asp Gly
195 200 205
Leu Asn Arg Phe Arg Ala Val Phe Gly Thr Leu Arg Leu Pro Glu Val
210 215 220
Ala Asp Ser Phe Arg Asp Asp Glu Ala Phe Ala Tyr Trp Arg Val Ala
225 230 235 240
Gly Pro Asn Pro Leu Leu Ile Arg Arg Val Asp Ala Leu Pro Ala Asn
245 250 255
Phe Pro Leu Gly Glu Glu Gln Phe Arg Arg Val Met Gly Ala Asp Asp
260 265 270
Ser Leu Leu Glu Ala Ala Ala Ser Arg Arg Leu Tyr Leu Leu Asp Tyr
275 280 285
Ala Glu Leu Gly Lys Leu Ala Pro Ser Gly Ala Val Asp Lys Leu Leu
290 295 300
Thr Gly Thr Gly Phe Ala Tyr Ala Pro Ile Ala Leu Phe Ala Leu Gly
305 310 315 320
Lys Asp Arg Ala Gly Leu Leu Pro Val Ala Ile Gln Cys Gly Gln Asp
325 330 335
Pro Ala Thr His Pro Met Phe Val Arg Pro Ala Glu Ser Glu Ser Asp
340 345 350
Leu Tyr Trp Gly Trp Gln Met Ala Lys Thr Val Val Gln Val Ala Glu
355 360 365
Glu Asn Tyr His Glu Met Phe Val His Leu Ala Gln Thr His Leu Val
370 375 380
Ser Glu Ala Phe Cys Leu Ala Thr Gln Arg Thr Leu Ala Pro Ser His
385 390 395 400
Pro Leu His Val Leu Leu Ala Pro His Phe Glu Gly Thr Leu Phe Ile
405 410 415
Asn Glu Gly Ala Ala Arg Ile Leu Leu Pro Ser Ala Gly Phe Ile Asp
420 425 430
Val Met Phe Ala Ala Pro Ile Gln Asp Thr Gln Ala Thr Ala Gly Gly
435 440 445
Asn Arg Leu Gly Phe Asp Phe Tyr Arg Gly Met Leu Pro Glu Ser Leu
450 455 460
Lys Ala Arg Asn Val Asp Asp Pro Ala Ala Leu Pro Asp Tyr Pro Tyr
465 470 475 480
Arg Asp Asp Gly Leu Leu Val Trp Asn Ala Ile Arg Gln Trp Ala Ala
485 490 495
Asp Tyr Val Ala Val Tyr Tyr Ala Ser Asp Gly Asp Val Thr Ala Asp
500 505 510
Val Glu Leu Ala Ala Trp Val Gly Glu Val Ile Gly Ser Gly Lys Val
515 520 525
Ala Gly Phe Arg Pro Ile Thr Gly Arg Ser Gln Leu Val Glu Val Leu
530 535 540
Thr Met Val Ile Phe Thr Ala Ser Ala Gln His Ala Ala Val Asn Phe
545 550 555 560
Pro Gln Pro Ser Met Met Thr Tyr Ala Pro Ala Ile Cys Ala Met Ser
565 570 575
Ala Ala Pro Ala Pro Asp Ser Pro Ser Gly Lys Ser Glu Ala Asp Trp
580 585 590
Leu Lys Met Met Pro Pro Thr Leu Val Ala Leu Glu Lys Val Asn Ile
595 600 605
Tyr His Leu Leu Gly Ser Val Tyr His Gly Arg Leu Gly Asp Tyr Arg
610 615 620
Gln Thr Gly Phe Pro Tyr Ala Pro Val Phe Ser Asp Arg Arg Val Thr
625 630 635 640
Ala Ser Gly Gly Pro Leu Glu Arg Phe Gln Ala Arg Leu Lys Glu Val
645 650 655
Glu Ala Thr Ile Arg Thr Arg Asn Gln Ala Arg Arg Lys Pro Tyr Glu
660 665 670
Tyr Leu Leu Pro Ser Arg Ile Pro Ala Ser Thr Asn Ile
675 680 685
<210> 24
<211> 880
<212> PRT
<213> 苦瓜（Momordica charantia）
<400> 24
Met Phe Gly Ile Gly Lys Ser Ile Ile Glu Gly Ala Val Asn Thr Thr
1 5 1015
Gly Asp Leu Ala Gly Ser Val Ile Asn Ala Gly Gly Asn Ile Val Gly
202530
Arg Val Thr Asn Ile Gly Gly Lys Lys Ile Lys Gly Thr Val Val Leu
354045
Met Arg Ser Asn Val Leu Asp Phe Thr Glu Phe His Ser Ser Leu Leu
505560
Asp Gly Val Thr Glu Leu Leu Gly Gly Gly Ile Ser Leu Gln Leu Ile
65707580
Ser Ala Thr His Ala Ser Asn Asp Ser Arg Gly Lys Val Gly Lys Gly
859095
Ala Phe Leu Glu Arg Trp Leu Thr Ser Val Pro Pro Leu Phe Ala Gly
100 105 110
Glu Ser Val Phe Gln Val Asn Phe Asp Trp Glu Glu Asn Phe Gly Phe
115 120 125
Pro Gly Ala Phe Phe Ile Lys Asn Gly His Thr Ser Glu Phe Phe Leu
130 135 140
Lys Ser Val Thr Leu Glu Asp Val Pro Gly Phe Gly Arg Val His Phe
145 150 155 160
Asp Cys Asn Ser Trp Val Tyr Pro Ser Arg Arg Tyr Lys Lys Asp Arg
165 170 175
Ile Phe Phe Ala Asn His Thr Cys Leu Pro Ile Asp Thr Pro Asp Ser
180 185 190
Leu Arg Lys Tyr Arg Glu Glu Glu Leu Leu Asn Leu Arg Gly Asp Gly
195 200 205
Thr Gly Glu Arg Lys Glu Trp Asp Arg Ile Tyr Asp Tyr Asp Val Tyr
210 215 220
Asn Asp Leu Cys Asp Pro Asn Gly Gly Pro Asn Leu Val Arg Pro Ile
225 230 235 240
Leu Gly Gly Ser Asp Gln Tyr Pro Tyr Pro Arg Arg Gly Arg Thr Gly
245 250 255
Arg Pro Pro Ala Arg Lys Asp His Lys Tyr Glu Ser Arg Leu Ser Asp
260 265 270
Val Met Ser Leu Asn Ile Tyr Val Pro Arg Asp Glu Asn Phe Gly His
275 280 285
Leu Lys Met Ala Asp Phe Leu Gly Asn Thr Leu Lys Val Leu Ser Thr
290 295 300
Ser Ile Gln Pro Gly Leu Glu Ser Ile Phe Asp Ser Thr Pro Gly Glu
305 310 315 320
Phe Asp Lys Phe Lys Glu Val Asp Asp Leu Phe Glu Arg Gly Phe Pro
325 330 335
Ile Pro Leu Asn Ile Phe Lys Asn Leu Thr Glu Asp Leu Ala Pro Pro
340 345 350
Leu Phe Lys Ala Phe Leu Arg Ser Asp Gly Glu Arg Phe Leu Lys Tyr
355 360 365
Pro Thr Pro Gln Val Ile Lys Asp Asn Lys Leu Gly Trp Arg Thr Asp
370 375 380
Glu Glu Phe Ala Arg Glu Met Ile Ala Gly Val Asn Pro Leu Ile Ile
385 390 395 400
Arg Arg Leu Glu Val Phe Pro Pro Leu Ser Lys Leu Asp Pro His Val
405 410 415
Tyr Gly Asn Gln Asn Ser Thr Met Thr Glu Glu Gln Ile Lys His Gly
420 425 430
Leu Asp Gly Leu Thr Val Asp Glu Ala Ile Lys Glu Asn Lys Leu Tyr
435 440 445
Ile Leu Asp His His Asp Ala Leu Met Pro Tyr Leu Arg Arg Ile Asn
450 455 460
Ser Thr Ser Thr Lys Thr Tyr Ala Thr Arg Thr Leu Leu Phe Leu Lys
465 470 475 480
Asp Asp Ser Thr Leu Lys Pro Leu Ala Ile Glu Leu Ser Leu Pro His
485 490 495
Pro Gln Gly Asp Glu His Gly Ala Ile Ser Lys Leu Tyr Phe Pro Ala
500 505 510
Glu Gly Arg Val Glu Ser Ala Ile Trp Gln Leu Ala Lys Ala Tyr Val
515 520 525
Ala Val Asn Asp Ser Gly Tyr His Gln Leu Asn Ser His Trp Leu His
530 535 540
Thr His Ala Val Leu Glu Pro Phe Val Ile Thr Thr His Arg Arg Leu
545 550 555 560
Ser Val Leu His Pro Ile His Lys Leu Leu Ala Pro His Tyr Lys Asp
565 570 575
Thr Met Phe Ile Asn Ala Ser Ala Arg Gln Val Leu Ile Asn Ala Gly
580 585 590
Gly Leu Ile Glu Ser Thr Gln Phe Pro Ala Lys Tyr Ala Met Glu Leu
595 600 605
Ser Ser Tyr Ile Tyr Lys Glu Trp Lys Phe Pro Asp Glu Ala Leu Pro
610 615 620
Thr Asn Leu Ile Lys Arg Gly Val Ala Ile Glu Asp Ser Gly Ser Pro
625 630 635 640
His Gly Val Arg Leu Leu Ile Asn Asp Tyr Pro Phe Ala Val Asp Gly
645 650 655
Leu Glu Ile Trp Ser Ala Ile Lys Thr Trp Val Thr Asp Tyr Cys Ser
660 665 670
Leu Tyr Tyr Lys Asp Asp Asp Ala Ile Arg Asn Asp Val Glu Leu Gln
675 680 685
Ser Trp Trp Lys Glu Leu Arg Glu Lys Gly His Thr Asp Lys Lys Asp
690 695 700
Glu Pro Trp Trp Pro Lys Met Gln Thr Phe Ser Glu Leu Ile Glu Ser
705 710 715 720
Cys Thr Ile Ile Ile Trp Ile Ser Ser Ala Leu His Ala Ala Val Asn
725 730 735
Phe Gly Gln Tyr Pro Tyr Gly Gly Tyr Val Pro Asn Arg Pro Thr Thr
740 745 750
Ser Arg Arg Phe Met Pro Glu Val Gly Thr Ala Glu Tyr Lys Glu Val
755 760 765
Glu Ser Asn Pro Glu Lys Ala Phe Leu Arg Thr Ile Ser Ser Gln Ile
770 775 780
Val Ala Leu Leu Gly Leu Ser Ile Ile Glu Ile Leu Ser Lys His Ala
785 790 795 800
Ser Asp Glu Val Tyr Leu Gly Gln Arg Ala Ser Ile Glu Trp Thr Ser
805 810 815
Asp Lys Ser Ala Ile Glu Ala Phe Glu Lys Phe Gly Lys Glu Leu Phe
820 825 830
Glu Val Glu Asp Arg Ile Met Arg Arg Asn Gln Asp Val Asn Leu Lys
835 840 845
Asn Arg Ala Gly Pro Val Asn Met Pro Tyr Thr Leu Leu Val Pro Ser
850 855 860
Ser Thr Glu Gly Leu Thr Gly Arg Gly Ile Pro Asn Ser Ile Ser Ile
865 870 875 880
<210> 25
<211> 674
<212> PRT
<213> 智人（Homo sapiens）
<400> 25
Met Pro Ser Tyr Thr Val Thr Val Ala Thr Gly Ser Gln Trp Phe Ala
1 5 1015
Gly Thr Asp Asp Tyr Ile Tyr Leu Ser Leu Val Gly Ser Ala Gly Cys
202530
Ser Glu Lys His Leu Leu Asp Lys Pro Phe Tyr Asn Asp Phe Glu Arg
354045
Gly Ala Val Asp Ser Tyr Asp Val Thr Val Asp Glu Glu Leu Gly Glu
505560
Ile Gln Leu Val Arg Ile Glu Lys Arg Lys Tyr Trp Leu Asn Asp Asp
65707580
Trp Tyr Leu Lys Tyr Ile Thr Leu Lys Thr Pro His Gly Asp Tyr Ile
859095
Glu Phe Pro Cys Tyr Arg Trp Ile Thr Gly Asp Val Glu Val Val Leu
100 105 110
Arg Asp Gly Arg Ala Lys Leu Ala Arg Asp Asp Gln Ile His Ile Leu
115 120 125
Lys Gln His Arg Arg Lys Glu Leu Glu Thr Arg Gln Lys Gln Tyr Arg
130 135 140
Trp Met Glu Trp Asn Pro Gly Phe Pro Leu Ser Ile Asp Ala Lys Cys
145 150 155 160
His Lys Asp Leu Pro Arg Asp Ile Gln Phe Asp Ser Glu Lys Gly Val
165 170 175
Asp Phe Val Leu Asn Tyr Ser Lys Ala Met Glu Asn Leu Phe Ile Asn
180 185 190
Arg Phe Met His Met Phe Gln Ser Ser Trp Asn Asp Phe Ala Asp Phe
195 200 205
Glu Lys Ile Phe Val Lys Ile Ser Asn Thr Ile Ser Glu Arg Val Met
210 215 220
Asn His Trp Gln Glu Asp Leu Met Phe Gly Tyr Gln Phe Leu Asn Gly
225 230 235 240
Cys Asn Pro Val Leu Ile Arg Arg Cys Thr Glu Leu Pro Glu Lys Leu
245 250 255
Pro Val Thr Thr Glu Met Val Glu Cys Ser Leu Glu Arg Gln Leu Ser
260 265 270
Leu Glu Gln Glu Val Gln Gln Gly Asn Ile Phe Ile Val Asp Phe Glu
275 280 285
Leu Leu Asp Gly Ile Asp Ala Asn Lys Thr Asp Pro Cys Thr Leu Gln
290 295 300
Phe Leu Ala Ala Pro Ile Cys Leu Leu Tyr Lys Asn Leu Ala Asn Lys
305 310 315 320
Ile Val Pro Ile Ala Ile Gln Leu Asn Gln Ile Pro Gly Asp Glu Asn
325 330 335
Pro Ile Phe Leu Pro Ser Asp Ala Lys Tyr Asp Trp Leu Leu Ala Lys
340 345 350
Ile Trp Val Arg Ser Ser Asp Phe His Val His Gln Thr Ile Thr His
355 360 365
Leu Leu Arg Thr His Leu Val Ser Glu Val Phe Gly Ile Ala Met Tyr
370 375 380
Arg Gln Leu Pro Ala Val His Pro Ile Phe Lys Leu Leu Val Ala His
385 390 395 400
Val Arg Phe Thr Ile Ala Ile Asn Thr Lys Ala Arg Glu Gln Leu Ile
405 410 415
Cys Glu Cys Gly Leu Phe Asp Lys Ala Asn Ala Thr Gly Gly Gly Gly
420 425 430
His Val Gln Met Val Gln Arg Ala Met Lys Asp Leu Thr Tyr Ala Ser
435 440 445
Leu Cys Phe Pro Glu Ala Ile Lys Ala Arg Gly Met Glu Ser Lys Glu
450 455 460
Asp Ile Pro Tyr Tyr Phe Tyr Arg Asp Asp Gly Leu Leu Val Trp Glu
465 470 475 480
Ala Ile Arg Thr Phe Thr Ala Glu Val Val Asp Ile Tyr Tyr Glu Gly
485 490 495
Asp Gln Val Val Glu Glu Asp Pro Glu Leu Gln Asp Phe Val Asn Asp
500 505 510
Val Tyr Val Tyr Gly Met Arg Gly Arg Lys Ser Ser Gly Phe Pro Lys
515 520 525
Ser Val Lys Ser Arg Glu Gln Leu Ser Glu Tyr Leu Thr Val Val Ile
530 535 540
Phe Thr Ala Ser Ala Gln His Ala Ala Val Asn Phe Gly Gln Tyr Asp
545 550 555 560
Trp Cys Ser Trp Ile Pro Asn Ala Pro Pro Thr Met Arg Ala Pro Pro
565 570 575
Pro Thr Ala Lys Gly Val Val Thr Ile Glu Gln Ile Val Asp Thr Leu
580 585 590
Pro Asp Arg Gly Arg Ser Cys Trp His Leu Gly Ala Val Trp Ala Leu
595 600 605
Ser Gln Phe Gln Glu Asn Glu Leu Phe Leu Gly Met Tyr Pro Glu Glu
610 615 620
His Phe Ile Glu Lys Pro Val Lys Glu Ala Met Ala Arg Phe Arg Lys
625 630 635 640
Asn Leu Glu Ala Ile Val Ser Val Ile Ala Glu Arg Asn Lys Lys Lys
645 650 655
Gln Leu Pro Tyr Tyr Tyr Leu Ser Pro Asp Arg Ile Pro Asn Ser Val
660 665 670
Ala Ile
<210> 26
<211> 1066
<212> PRT
<213> 海鞭（Plexaura homomalla）
<400> 26
Met Thr Trp Lys Asn Phe Gly Phe Glu Ile Phe Gly Glu Lys Tyr Gly
1 5 1015
Gln Glu Glu Leu Glu Lys Arg Ile Lys Asp Glu His Thr Pro Pro Pro
202530
Asp Ser Pro Val Phe Gly Gly Leu Lys Leu Lys Leu Lys Lys Glu Lys
354045
Phe Lys Thr Leu Phe Thr Leu Gly Thr Thr Leu Lys Gly Phe Arg Arg
505560
Ala Thr His Thr Val Gly Thr Gly Gly Ile Gly Glu Ile Thr Ile Val
65707580
Asn Asp Pro Lys Phe Pro Glu His Glu Phe Phe Thr Ala Gly Arg Thr
859095
Phe Pro Ala Arg Leu Arg His Ala Asn Leu Lys Tyr Pro Asp Asp Ala
100 105 110
Gly Ala Asp Ala Arg Ser Phe Ser Ile Lys Phe Ala Asp Ser Asp Ser
115 120 125
Asp Gly Pro Leu Asp Ile Val Met Asn Thr Gly Glu Ala Asn Ile Phe
130 135 140
Trp Asn Ser Pro Ser Leu Glu Asp Phe Val Pro Val Glu Glu Gly Asp
145 150 155 160
Ala Ala Glu Glu Tyr Val Tyr Lys Asn Pro Tyr Tyr Tyr Tyr Asn Leu
165 170 175
Val Glu Ala Leu Arg Arg Ala Pro Asp Thr Phe Ala His Leu Tyr Tyr
180 185 190
Tyr Ser Gln Val Thr Met Pro Phe Lys Ala Lys Asp Gly Lys Val Arg
195 200 205
Tyr Cys Arg Tyr Arg Ala Leu Pro Gly Asp Val Asp Ile Lys Glu Glu
210 215 220
Asp Glu Ser Gly Arg Leu Thr Glu Glu Glu Gln Arg Lys Ile Trp Ile
225 230 235 240
Phe Ser Arg His Glu Asn Glu Lys Arg Pro Asp Asp Tyr Leu Arg Lys
245 250 255
Glu Tyr Val Glu Arg Leu Gln Lys Gly Pro Val Asn Tyr Arg Leu Gln
260 265 270
Ile Gln Ile His Glu Ala Ser Pro Asp Asp Thr Ala Thr Ile Phe His
275 280 285
Ala Gly Ile Leu Trp Asp Lys Glu Thr His Pro Trp Phe Asp Leu Ala
290 295 300
Lys Val Ser Ile Lys Thr Pro Leu Ser Pro Asp Val Leu Glu Lys Thr
305 310 315 320
Ala Phe Asn Ile Ala Asn Gln Pro Ala Ser Leu Gly Leu Leu Glu Ala
325 330 335
Lys Ser Pro Glu Asp Tyr Asn Ser Ile Gly Glu Leu Arg Val Ala Val
340 345 350
Tyr Thr Trp Val Gln His Leu Arg Lys Leu Lys Ile Gly Ser Leu Val
355 360 365
Pro Ala Gly Gln Asn Ala Ile Tyr Asn Val Glu Val Glu Thr Gly Asp
370 375 380
Arg Glu His Ala Gly Thr Asp Ala Thr Ile Thr Ile Arg Ile Thr Gly
385 390 395 400
Ala Lys Gly Arg Thr Asp Tyr Leu Lys Leu Asp Lys Trp Phe His Asn
405 410 415
Asp Phe Glu Ala Gly Ser Lys Glu Gln Tyr Thr Val Gln Gly Phe Asp
420 425 430
Val Gly Asp Ile Gln Leu Ile Glu Leu His Ser Asp Gly Gly Gly Tyr
435 440 445
Trp Ser Gly Asp Pro Asp Trp Phe Val Asn Arg Val Ile Ile Ile Ser
450 455 460
Ser Thr Gln Asp Arg Val Tyr Ser Phe Pro Cys Phe Arg Trp Val Ile
465 470 475 480
Lys Asp Met Val Leu Phe Pro Gly Glu Ala Thr Leu Pro Phe Asn Glu
485 490 495
Val Pro Ala Ile Val Ser Glu Gln Arg Gln Lys Glu Leu Glu Gln Arg
500 505 510
Lys Leu Thr Tyr Gln Trp Asp Tyr Val Ser Asp Asp Met Pro Gly Asn
515 520 525
Ile Lys Ala Lys Thr His Asp Asp Leu Pro Arg Asp Val Gln Phe Thr
530 535 540
Asp Glu Lys Ser Arg Ser Tyr Gln Glu Ser Arg Lys Ala Ala Leu Val
545 550 555 560
Asn Leu Gly Ile Gly Ser Leu Phe Thr Met Phe Glu Asn Trp Asp Ser
565 570 575
Tyr Asp Asp Tyr His Ile Leu Tyr Arg Asn Trp Ile Leu Gly Gly Thr
580 585 590
Pro Asn Met Ala Asp Arg Trp His Glu Asp Arg Trp Phe Gly Tyr Gln
595 600 605
Phe Leu Asn Gly Ala Asn Pro Val Ile Leu Thr Arg Cys Asp Ala Leu
610 615 620
Pro Ser Asn Phe Pro Val Thr Asn Glu His Val Asn Ala Ser Leu Asp
625 630 635 640
Arg Gly Lys Asn Leu Asp Glu Glu Ile Lys Asp Gly His Ile Tyr Ile
645 650 655
Val Asp Phe Lys Val Leu Val Gly Ala Lys Ser Tyr Gly Gly Pro Val
660 665 670
Leu Glu Asp Ile Gly Tyr Lys Val Pro Asp His Leu Lys His Asp Glu
675 680 685
Ala Asp Ile Arg Tyr Cys Ala Ala Pro Leu Ala Leu Phe Tyr Val Asn
690 695 700
Lys Leu Gly His Leu Met Pro Ile Ala Ile Gln Ile Asn Gln Glu Pro
705 710 715 720
Gly Pro Glu Asn Pro Ile Trp Thr Pro His Glu Glu Asn Glu His Asp
725 730 735
Trp Met Met Ala Lys Phe Trp Leu Gly Val Ala Glu Ser Asn Phe His
740 745 750
Gln Leu Asn Thr His Leu Leu Arg Thr His Leu Thr Thr Glu Ser Phe
755 760 765
Ala Leu Ser Thr Trp Arg Asn Leu Ala Ser Ala His Pro Val Phe Lys
770 775 780
Leu Leu Gln Pro His Ile Tyr Gly Val Leu Ala Ile Asp Thr Ile Gly
785 790 795 800
Arg Lys Glu Leu Ile Gly Ser Gly Gly Ile Val Asp Gln Ser Leu Ser
805 810 815
Leu Gly Gly Gly Gly His Val Thr Phe Met Glu Lys Cys Phe Lys Glu
820 825 830
Val Asn Leu Gln Asp Tyr His Leu Pro Asn Ala Leu Lys Lys Arg Gly
835 840 845
Val Asp Asp Pro Ser Lys Leu Pro Gly Phe Tyr Tyr Arg Asp Asp Gly
850 855 860
Leu Ala Leu Trp Glu Ala Ile Glu Thr Phe Ile Gly Glu Ile Ile Ala
865 870 875 880
Ile Phe Tyr Lys Asn Asp Asp Asp Val Lys Arg Asp Asn Glu Ile Gln
885 890 895
Ser Trp Ile Tyr Asp Val His Lys Asn Gly Trp Arg Val Asn Pro Gly
900 905 910
His Gln Asp His Gly Val Pro Ala Ser Phe Glu Ser Arg Glu Gln Leu
915 920 925
Lys Glu Val Leu Thr Ser Leu Val Phe Thr Phe Ser Cys Gln His Ala
930 935 940
Ala Val Asn Phe Ser Gln Lys Asp His Tyr Gly Phe Thr Pro Asn Ala
945 950 955 960
Pro Ala Val Leu Arg His Pro Pro Pro Lys Lys Lys Gly Glu Ala Thr
965 970 975
Leu Gln Ser Ile Leu Ser Thr Leu Pro Ser Lys Ser Gln Ala Ala Lys
980 985 990
Ala Ile Ala Thr Val Tyr Ile Leu Thr Lys Phe Ser Glu Asp Glu Arg
995 10001005
Tyr Leu Gly Asn Tyr Ser Ala Thr Ala Trp Glu Asp Lys Asp Ala
101010151020
Leu Asp Ala Ile Asn Arg Phe Gln Asp Lys Leu Glu Asp Ile Ser
102510301035
Lys Lys Ile Lys Gln Arg Asn Glu Asn Leu Glu Val Pro Tyr Ile
104010451050
Tyr Leu Leu Pro Glu Arg Ile Pro Asn Gly Thr Ala Ile
105510601065
<210> 27
<211> 663
<212> PRT
<213> 智人（Homo sapiens）
<400> 27
Met Gly Arg Tyr Arg Ile Arg Val Ala Thr Gly Ala Trp Leu Phe Ser
1 5 1015
Gly Ser Tyr Asn Arg Val Gln Leu Trp Leu Val Gly Thr Arg Gly Glu
202530
Ala Glu Leu Glu Leu Gln Leu Arg Pro Ala Arg Gly Glu Glu Glu Glu
354045
Phe Asp His Asp Val Ala Glu Asp Leu Gly Leu Leu Gln Phe Val Arg
505560
Leu Arg Lys His His Trp Leu Val Asp Asp Ala Trp Phe Cys Asp Arg
65707580
Ile Thr Val Gln Gly Pro Gly Ala Cys Ala Glu Val Ala Phe Pro Cys
859095
Tyr Arg Trp Val Gln Gly Glu Asp Ile Leu Ser Leu Pro Glu Gly Thr
100 105 110
Ala Arg Leu Pro Gly Asp Asn Ala Leu Asp Met Phe Gln Lys His Arg
115 120 125
Glu Lys Glu Leu Lys Asp Arg Gln Gln Ile Tyr Cys Trp Ala Thr Trp
130 135 140
Lys Glu Gly Leu Pro Leu Thr Ile Ala Ala Asp Arg Lys Asp Asp Leu
145 150 155 160
Pro Pro Asn Met Arg Phe His Glu Glu Lys Arg Leu Asp Phe Glu Trp
165 170 175
Thr Leu Lys Ala Gly Ala Leu Glu Met Ala Leu Lys Arg Val Tyr Thr
180 185 190
Leu Leu Ser Ser Trp Asn Cys Leu Glu Asp Phe Asp Gln Ile Phe Trp
195 200 205
Gly Gln Lys Ser Ala Leu Ala Glu Lys Val Arg Gln Cys Trp Gln Asp
210 215 220
Asp Glu Leu Phe Ser Tyr Gln Phe Leu Asn Gly Ala Asn Pro Met Leu
225 230 235 240
Leu Arg Arg Ser Thr Ser Leu Pro Ser Arg Leu Val Leu Pro Ser Gly
245 250 255
Met Glu Glu Leu Gln Ala Gln Leu Glu Lys Glu Leu Gln Asn Gly Ser
260 265 270
Leu Phe Glu Ala Asp Phe Ile Leu Leu Asp Gly Ile Pro Ala Asn Val
275 280 285
Ile Arg Gly Glu Lys Gln Tyr Leu Ala Ala Pro Leu Val Met Leu Lys
290 295 300
Met Glu Pro Asn Gly Lys Leu Gln Pro Met Val Ile Gln Ile Gln Pro
305 310 315 320
Pro Asn Pro Ser Ser Pro Thr Pro Thr Leu Phe Leu Pro Ser Asp Pro
325 330 335
Pro Leu Ala Trp Leu Leu Ala Lys Ser Trp Val Arg Asn Ser Asp Phe
340 345 350
Gln Leu His Glu Ile Gln Tyr His Leu Leu Asn Thr His Leu Val Ala
355 360 365
Glu Val Ile Ala Val Ala Thr Met Arg Cys Leu Pro Gly Leu His Pro
370 375 380
Ile Phe Lys Phe Leu Ile Pro His Ile Arg Tyr Thr Met Glu Ile Asn
385 390 395 400
Thr Arg Ala Arg Thr Gln Leu Ile Ser Asp Gly Gly Ile Phe Asp Lys
405 410 415
Ala Val Ser Thr Gly Gly Gly Gly His Val Gln Leu Leu Arg Arg Ala
420 425 430
Ala Ala Gln Leu Thr Tyr Cys Ser Leu Cys Pro Pro Asp Asp Leu Ala
435 440 445
Asp Arg Gly Leu Leu Gly Leu Pro Gly Ala Leu Tyr Ala His Asp Ala
450 455 460
Leu Arg Leu Trp Glu Ile Ile Ala Arg Tyr Val Glu Gly Ile Val His
465 470 475 480
Leu Phe Tyr Gln Arg Asp Asp Ile Val Lys Gly Asp Pro Glu Leu Gln
485 490 495
Ala Trp Cys Arg Glu Ile Thr Glu Val Gly Leu Cys Gln Ala Gln Asp
500 505 510
Arg Gly Phe Pro Val Ser Phe Gln Ser Gln Ser Gln Leu Cys His Phe
515 520 525
Leu Thr Met Cys Val Phe Thr Cys Thr Ala Gln His Ala Ala Ile Asn
530 535 540
Gln Gly Gln Leu Asp Trp Tyr Ala Trp Val Pro Asn Ala Pro Cys Thr
545 550 555 560
Met Arg Met Pro Pro Pro Thr Thr Lys Glu Asp Val Thr Met Ala Thr
565 570 575
Val Met Gly Ser Leu Pro Asp Val Arg Gln Ala Cys Leu Gln Met Ala
580 585 590
Ile Ser Trp His Leu Ser Arg Arg Gln Pro Asp Met Val Pro Leu Gly
595 600 605
His His Lys Glu Lys Tyr Phe Ser Gly Pro Lys Pro Lys Ala Val Leu
610 615 620
Asn Gln Phe Arg Thr Asp Leu Glu Lys Leu Glu Lys Glu Ile Thr Ala
625 630 635 640
Arg Asn Glu Gln Leu Asp Trp Pro Tyr Glu Tyr Leu Lys Pro Ser Cys
645 650 655
Ile Glu Asn Ser Val Thr Ile
660
<210> 28
<211> 701
<212> PRT
<213> 智人（Homo sapiens）
<400> 28
Met Ala Thr Tyr Lys Val Arg Val Ala Thr Gly Thr Asp Leu Leu Ser
1 5 1015
Gly Thr Arg Asp Ser Ile Ser Leu Thr Ile Val Gly Thr Gln Gly Glu
202530
Ser His Lys Gln Leu Leu Asn His Phe Gly Arg Asp Phe Ala Thr Gly
354045
Ala Val Gly Gln Tyr Thr Val Gln Cys Pro Gln Asp Leu Gly Glu Leu
505560
Ile Ile Ile Arg Leu His Lys Glu Arg Tyr Ala Phe Phe Pro Lys Asp
65707580
Pro Trp Tyr Cys Asn Tyr Val Gln Ile Cys Ala Pro Asn Gly Arg Ile
859095
Tyr His Phe Pro Ala Tyr Gln Trp Met Asp Gly Tyr Glu Thr Leu Ala
100 105 110
Leu Arg Glu Ala Thr Gly Lys Thr Thr Ala Asp Asp Ser Leu Pro Val
115 120 125
Leu Leu Glu His Arg Lys Glu Glu Ile Arg Ala Lys Gln Asp Phe Tyr
130 135 140
His Trp Arg Val Phe Leu Pro Gly Leu Pro Ser Tyr Val His Ile Pro
145 150 155 160
Ser Tyr Arg Pro Pro Val Arg Arg His Arg Asn Pro Asn Arg Pro Glu
165 170 175
Trp Asn Gly Tyr Ile Pro Gly Phe Pro Ile Leu Ile Asn Phe Lys Ala
180 185 190
Thr Lys Phe Leu Asn Leu Asn Leu Arg Tyr Ser Phe Leu Lys Thr Ala
195 200 205
Ser Phe Phe Val Arg Leu Gly Pro Met Ala Leu Ala Phe Lys Val Arg
210 215 220
Gly Leu Leu Asp Cys Lys His Ser Trp Lys Arg Leu Lys Asp Ile Arg
225 230 235 240
Lys Ile Phe Pro Gly Lys Lys Ser Val Val Ser Glu Tyr Val Ala Glu
245 250 255
His Trp Ala Glu Asp Thr Phe Phe Gly Tyr Gln Tyr Leu Asn Gly Val
260 265 270
Asn Pro Gly Leu Ile Arg Arg Cys Thr Arg Ile Pro Asp Lys Phe Pro
275 280 285
Val Thr Asp Asp Met Val Ala Pro Phe Leu Gly Glu Gly Thr Cys Leu
290 295 300
Gln Ala Glu Leu Glu Lys Gly Asn Ile Tyr Leu Ala Asp Tyr Arg Ile
305 310 315 320
Met Glu Gly Ile Pro Thr Val Glu Leu Ser Gly Arg Lys Gln His His
325 330 335
Cys Ala Pro Leu Cys Leu Leu His Phe Gly Pro Glu Gly Lys Met Met
340 345 350
Pro Ile Ala Ile Gln Leu Ser Gln Thr Pro Gly Pro Asp Cys Pro Ile
355 360 365
Phe Leu Pro Ser Asp Ser Glu Trp Asp Trp Leu Leu Ala Lys Thr Trp
370 375 380
Val Arg Tyr Ala Glu Phe Tyr Ser His Glu Ala Ile Ala His Leu Leu
385 390 395 400
Glu Thr His Leu Ile Ala Glu Ala Phe Cys Leu Ala Leu Leu Arg Asn
405 410 415
Leu Pro Met Cys His Pro Leu Tyr Lys Leu Leu Ile Pro His Thr Arg
420 425 430
Tyr Thr Val Gln Ile Asn Ser Ile Gly Arg Ala Val Leu Leu Asn Glu
435 440 445
Gly Gly Leu Ser Ala Lys Gly Met Ser Leu Gly Val Glu Gly Phe Ala
450 455 460
Gly Val Met Val Arg Ala Leu Ser Glu Leu Thr Tyr Asp Ser Leu Tyr
465 470 475 480
Leu Pro Asn Asp Phe Val Glu Arg Gly Val Gln Asp Leu Pro Gly Tyr
485 490 495
Tyr Tyr Arg Asp Asp Ser Leu Ala Val Trp Asn Ala Leu Glu Lys Tyr
500 505 510
Val Thr Glu Ile Ile Thr Tyr Tyr Tyr Pro Ser Asp Ala Ala Val Glu
515 520 525
Gly Asp Pro Glu Leu Gln Ser Trp Val Gln Glu Ile Phe Lys Glu Cys
530 535 540
Leu Leu Gly Arg Glu Ser Ser Gly Phe Pro Arg Cys Leu Arg Thr Val
545 550 555 560
Pro Glu Leu Ile Arg Tyr Val Thr Ile Val Ile Tyr Thr Cys Ser Ala
565 570 575
Lys His Ala Ala Val Asn Thr Gly Gln Met Glu Phe Thr Ala Trp Met
580 585 590
Pro Asn Phe Pro Ala Ser Met Arg Asn Pro Pro Ile Gln Thr Lys Gly
595 600 605
Leu Thr Thr Leu Glu Thr Phe Met Asp Thr Leu Pro Asp Val Lys Thr
610 615 620
Thr Cys Ile Thr Leu Leu Val Leu Trp Thr Leu Ser Arg Glu Pro Asp
625 630 635 640
Asp Arg Arg Pro Leu Gly His Phe Pro Asp Ile His Phe Val Glu Glu
645 650 655
Ala Pro Arg Arg Ser Ile Glu Ala Phe Arg Gln Arg Leu Asn Gln Ile
660 665 670
Ser His Asp Ile Arg Gln Arg Asn Lys Cys Leu Pro Ile Pro Tyr Tyr
675 680 685
Tyr Leu Asp Pro Val Leu Ile Glu Asn Ser Ile Ser Ile
690 695 700
<210> 29
<211> 662
<212> PRT
<213> 智人（Homo sapiens）
<400> 29
Met Gly Leu Tyr Arg Ile Arg Val Ser Thr Gly Ala Ser Leu Tyr Ala
1 5 1015
Gly Ser Asn Asn Gln Val Gln Leu Trp Leu Val Gly Gln His Gly Glu
202530
Ala Ala Leu Gly Lys Arg Leu Trp Pro Ala Arg Gly Lys Glu Thr Glu
354045
Leu Lys Val Glu Val Pro Glu Tyr Leu Gly Pro Leu Leu Phe Val Lys
505560
Leu Arg Lys Arg His Leu Leu Lys Asp Asp Ala Trp Phe Cys Asn Trp
65707580
Ile Ser Val Gln Gly Pro Gly Ala Gly Asp Glu Val Arg Phe Pro Cys
859095
Tyr Arg Trp Val Glu Gly Asn Gly Val Leu Ser Leu Pro Glu Gly Thr
100 105 110
Gly Arg Thr Val Gly Glu Asp Pro Gln Gly Leu Phe Gln Lys His Arg
115 120 125
Glu Glu Glu Leu Glu Glu Arg Arg Lys Leu Tyr Arg Trp Gly Asn Trp
130 135 140
Lys Asp Gly Leu Ile Leu Asn Met Ala Gly Ala Lys Leu Tyr Asp Leu
145 150 155 160
Pro Val Asp Glu Arg Phe Leu Glu Asp Lys Arg Val Asp Phe Glu Val
165 170 175
Ser Leu Ala Lys Gly Leu Ala Asp Leu Ala Ile Lys Asp Ser Leu Asn
180 185 190
Val Leu Thr Cys Trp Lys Asp Leu Asp Asp Phe Asn Arg Ile Phe Trp
195 200 205
Cys Gly Gln Ser Lys Leu Ala Glu Arg Val Arg Asp Ser Trp Lys Glu
210 215 220
Asp Ala Leu Phe Gly Tyr Gln Phe Leu Asn Gly Ala Asn Pro Val Val
225 230 235 240
Leu Arg Arg Ser Ala His Leu Pro Ala Arg Leu Val Phe Pro Pro Gly
245 250 255
Met Glu Glu Leu Gln Ala Gln Leu Glu Lys Glu Leu Glu Gly Gly Thr
260 265 270
Leu Phe Glu Ala Asp Phe Ser Leu Leu Asp Gly Ile Lys Ala Asn Val
275 280 285
Ile Leu Cys Ser Gln Gln His Leu Ala Ala Pro Leu Val Met Leu Lys
290 295 300
Leu Gln Pro Asp Gly Lys Leu Leu Pro Met Val Ile Gln Leu Gln Leu
305 310 315 320
Pro Arg Thr Gly Ser Pro Pro Pro Pro Leu Phe Leu Pro Thr Asp Pro
325 330 335
Pro Met Ala Trp Leu Leu Ala Lys Cys Trp Val Arg Ser Ser Asp Phe
340 345 350
Gln Leu His Glu Leu Gln Ser His Leu Leu Arg Gly His Leu Met Ala
355 360 365
Glu Val Ile Val Val Ala Thr Met Arg Cys Leu Pro Ser Ile His Pro
370 375 380
Ile Phe Lys Leu Ile Ile Pro His Leu Arg Tyr Thr Leu Glu Ile Asn
385 390 395 400
Val Arg Ala Arg Thr Gly Leu Val Ser Asp Met Gly Ile Phe Asp Gln
405 410 415
Ile Met Ser Thr Gly Gly Gly Gly His Val Gln Leu Leu Lys Gln Ala
420 425 430
Gly Ala Phe Leu Thr Tyr Ser Ser Phe Cys Pro Pro Asp Asp Leu Ala
435 440 445
Asp Arg Gly Leu Leu Gly Val Lys Ser Ser Phe Tyr Ala Gln Asp Ala
450 455 460
Leu Arg Leu Trp Glu Ile Ile Tyr Arg Tyr Val Glu Gly Ile Val Ser
465 470 475 480
Leu His Tyr Lys Thr Asp Val Ala Val Lys Asp Asp Pro Glu Leu Gln
485 490 495
Thr Trp Cys Arg Glu Ile Thr Glu Ile Gly Leu Gln Gly Ala Gln Asp
500 505 510
Arg Gly Phe Pro Val Ser Leu Gln Ala Arg Asp Gln Val Cys His Phe
515 520 525
Val Thr Met Cys Ile Phe Thr Cys Thr Gly Gln His Ala Ser Val His
530 535 540
Leu Gly Gln Leu Asp Trp Tyr Ser Trp Val Pro Asn Ala Pro Cys Thr
545 550 555 560
Met Arg Leu Pro Pro Pro Thr Thr Lys Asp Ala Thr Leu Glu Thr Val
565 570 575
Met Ala Thr Leu Pro Asn Phe His Gln Ala Ser Leu Gln Met Ser Ile
580 585 590
Thr Trp Gln Leu Gly Arg Arg Gln Pro Val Met Val Ala Val Gly Gln
595 600 605
His Glu Glu Glu Tyr Phe Ser Gly Pro Glu Pro Lys Ala Val Leu Lys
610 615 620
Lys Phe Arg Glu Glu Leu Ala Ala Leu Asp Lys Glu Ile Glu Ile Arg
625 630 635 640
Asn Ala Lys Leu Asp Met Pro Tyr Glu Tyr Leu Arg Pro Ser Val Val
645 650 655
Glu Asn Ser Val Ala Ile
660
<210> 30
<211> 937
<212> PRT
<213> 小立碗藓（Physcomitrella patens）
<400> 30
Met Met Leu Asn Arg Leu Glu Thr Gly Ser Leu Ala Ser Ala Phe Arg
1 5 1015
Gly Ser Arg Glu Glu Phe Val Val Ser Arg Gly Arg Ser Pro Gly Arg
202530
Ser Gln Ser Ile Gln Val Arg Ala Ser Ser Gly Gly Asp Pro Ala Gly
354045
Trp Leu Gln Thr Ala Ser Lys Gln Leu Gly Lys Leu Ser Ser Phe Gly
505560
Glu Lys Arg Lys Thr Ala Thr Ser Thr Ser Thr Arg Gly Pro Ser Gly
65707580
Asp Asn Val Gln Tyr Thr Gly Val Ala Thr Thr Met Lys Lys Leu Lys
859095
Val Leu Asp Leu Ile Asp Arg Val Ala Asp Ile Gln Asp Asp Thr Ser
100 105 110
Glu Ile Val Gly Gly Lys Arg Val Thr Val Gln Leu Val Ser Lys Asp
115 120 125
Val Asp Pro Lys Thr Gly Glu Ser Met Lys Ser Ser Glu Val Ile Phe
130 135 140
Pro Asn Trp Ala Gly Leu Glu Gly Pro Ala Ala Ser Leu Ile Asp Phe
145 150 155 160
Val Leu Glu Phe Thr Val Pro Lys Ser Phe Gly Val Pro Gly Ala Ile
165 170 175
Leu Val Lys Asn Ala His Pro Asn Glu Phe Leu Leu Val Ser Phe Glu
180 185 190
Leu Glu Leu His Asp Lys Ser Lys Ala His Tyr Val Thr Asn Ser Trp
195 200 205
Val Tyr Asn Thr Glu Lys Thr Gly Ala Arg Ile Phe Phe Gln Asn Thr
210 215 220
Ala Tyr Leu Pro Asp Glu Thr Pro Ala Ser Leu Lys Ala Leu Arg Glu
225 230 235 240
Gln Glu Leu Ile Asn Leu Arg Gly Asp Gly Thr Gly Glu Arg Gln Ile
245 250 255
Gly Asp Arg Ile Tyr Asp Tyr Ala Val Tyr Asn Asp Leu Gly Asn Ile
260 265 270
Glu Gln Asn Glu Lys Phe Glu Arg Pro Asn Leu Gly Gly Asn Asp Met
275 280 285
Tyr His Phe Pro Arg Arg Met Arg Thr Gly Arg Arg Asn Thr Thr Val
290 295 300
Glu Ala Lys Lys Phe Pro Gly Met Val Tyr Glu Thr Arg Lys Thr Lys
305 310 315 320
Gly Asp Phe Tyr Ile Pro Arg Asp Glu Ala Phe Glu Arg Ala Lys Met
325 330 335
Ser Asp Phe Leu Ala Asp Gly Phe Arg Ser Ile Gly His Ser Val Ser
340 345 350
Ser Lys Val Thr Gly Phe Val Thr Arg Lys Gln Glu Phe Asp Thr Val
355 360 365
Glu Glu Ile Lys Lys Leu Tyr Ala Lys Lys Gly Glu Lys Val Gly Gly
370 375 380
Ile Asn Asn Val Leu Pro Asp Lys Glu Asp Ile Pro Glu Gln Glu Gln
385 390 395 400
Tyr Pro Leu Val Phe Leu Gln Glu Val Leu Lys Pro Asp Gly Lys Met
405 410 415
Glu His Pro Leu Leu Tyr Pro Leu Pro Gln Leu Leu Gln Ala Asp Asp
420 425 430
Thr Ser Trp Arg Ser Asn Asp Glu Phe Ala Arg Glu Phe Leu Ala Gly
435 440 445
Leu Asn Pro Val Met Ile Thr Arg Val Lys Phe Pro Ile Arg Ser Ser
450 455 460
Leu Asp Pro Ala Glu Phe Gly Asp Pro Thr Ser Ala Ile Thr Lys Asp
465 470 475 480
His Ile Glu Gly Ser Leu Glu Gly Leu Ser Val Glu Glu Ala Val Thr
485 490 495
Ser Asn Arg Leu Phe Val Val Asp Tyr His Asp Ala Phe Leu Pro Phe
500 505 510
Val Ala Lys Ile Asn Ala Gln Gln Asn Ser Ala Thr Tyr Ala Thr Arg
515 520 525
Thr Leu Leu Phe Leu Ser Lys Asp Gly Ile Leu Lys Leu Leu Ala Ile
530 535 540
Glu Leu Ala Leu Pro Pro Lys Thr Val Gly Glu Glu Arg Ile Thr Arg
545 550 555 560
Val Leu Thr Thr Arg Lys Asp Asp Gln Leu Trp Lys Val Asn Trp Glu
565 570 575
Trp Glu Leu Ala Lys Ala His Val Ser Asn Asn Asp Ile Thr Ala His
580 585 590
Gln Val Phe Ser His Phe Ser Arg Cys His Ala Val Thr Glu Ala Val
595 600 605
Ile Ile Cys Ser Asn Arg Asn Leu Ser Lys Leu His Pro Leu Met Gln
610 615 620
Leu Leu Ala Pro His Phe Lys Ser Thr Leu Glu Ile Asn Arg Gln Ala
625 630 635 640
Arg Ala Thr Leu Ile Ala Ala Gly Gly Ser Ile Glu Thr His Phe Thr
645 650 655
Thr Arg Ala Tyr Ser Leu Glu Met Ala Ala Val Asn Tyr Lys Asp Thr
660 665 670
Trp Thr Phe Glu Ser Gln Ala Leu Pro Thr Asp Leu Val Ala Arg Gly
675 680 685
Met Ala Val Pro Asp Pro Asp Ser Pro His Gly Val Arg Leu Val Val
690 695 700
Glu Asp Tyr Pro Tyr Ala Ala Asp Gly Leu Glu Leu Trp Gly Ala Leu
705 710 715 720
Lys Ala Trp His Lys Glu Tyr Val Asp Ile Tyr Tyr Lys Asp Asp Ala
725 730 735
Ala Val Leu Gln Asp Ser Glu Leu Met Thr Trp Trp Thr Glu Met Arg
740 745 750
Glu Lys Ala His Glu Asp Lys Lys Asp Ser His Gly Trp Pro Glu Leu
755 760 765
Asn Ser Lys Glu Ala Leu Val Asp Ile Leu Thr Thr Val Ile Trp Ile
770 775 780
Pro Ser Cys Leu His Ala Ala Val Asn Phe Gly Gln Tyr Asp Phe Ala
785 790 795 800
Gly Phe Met Pro His His Pro Thr Leu Thr Arg Arg Leu Leu Pro Glu
805 810 815
His Gly Asn Glu Lys Asp Lys Ala Asp Phe Asn Lys Asn Pro Glu Lys
820 825 830
Tyr Tyr Leu Thr Ser Ile Ser Asn Ile Asp Ser Thr Thr Thr Ala Met
835 840 845
Ser Val Tyr Glu Val Leu Ser Ala His Cys Pro Ile Glu Glu Tyr Ile
850 855 860
Gly Glu Arg Arg Gly Asn Trp Thr Asn Asn Glu Lys Val Leu Ala Ala
865 870 875 880
Phe Lys Gly Phe Lys Glu Ser Val Asn Glu Ala Asp Ala Val Met Arg
885 890 895
Ala Arg Asn Ala Asp Pro Lys Leu Arg Asn Arg Gly Gly Pro Val Lys
900 905 910
Met Pro Tyr Gln Leu Leu Arg Pro His Ser Lys Pro Gly Val Thr Ser
915 920 925
Met Gly Val Pro Asn Ser Ile Thr Ile
930 935
<210> 31
<211> 676
<212> PRT
<213> 智人（Homo sapiens）
<400> 31
Met Ala Glu Phe Arg Val Arg Val Ser Thr Gly Glu Ala Phe Gly Ala
1 5 1015
Gly Thr Trp Asp Lys Val Ser Val Ser Ile Val Gly Thr Arg Gly Glu
202530
Ser Pro Pro Leu Pro Leu Asp Asn Leu Gly Lys Glu Phe Thr Ala Gly
354045
Ala Glu Glu Asp Phe Gln Val Thr Leu Pro Glu Asp Val Gly Arg Val
505560
Leu Leu Leu Arg Val His Lys Ala Pro Pro Val Leu Pro Leu Leu Gly
65707580
Pro Leu Ala Pro Asp Ala Trp Phe Cys Arg Trp Phe Gln Leu Thr Pro
859095
Pro Arg Gly Gly His Leu Leu Phe Pro Cys Tyr Gln Trp Leu Glu Gly
100 105 110
Ala Gly Thr Leu Val Leu Gln Glu Gly Thr Ala Lys Val Ser Trp Ala
115 120 125
Asp His His Pro Val Leu Gln Gln Gln Arg Gln Glu Glu Leu Gln Ala
130 135 140
Arg Gln Glu Met Tyr Gln Trp Lys Ala Tyr Asn Pro Gly Trp Pro His
145 150 155 160
Cys Leu Asp Glu Lys Thr Val Glu Asp Leu Glu Leu Asn Ile Lys Tyr
165 170 175
Ser Thr Ala Lys Asn Ala Asn Phe Tyr Leu Gln Ala Gly Ser Ala Phe
180 185 190
Ala Glu Met Lys Ile Lys Gly Leu Leu Asp Arg Lys Gly Leu Trp Arg
195 200 205
Ser Leu Asn Glu Met Lys Arg Ile Phe Asn Phe Arg Arg Thr Pro Ala
210 215 220
Ala Glu His Ala Phe Glu His Trp Gln Glu Asp Ala Phe Phe Ala Ser
225 230 235 240
Gln Phe Leu Asn Gly Leu Asn Pro Val Leu Ile Arg Arg Cys His Tyr
245 250 255
Leu Pro Lys Asn Phe Pro Val Thr Asp Ala Met Val Ala Ser Val Leu
260 265 270
Gly Pro Gly Thr Ser Leu Gln Ala Glu Leu Glu Lys Gly Ser Leu Phe
275 280 285
Leu Val Asp His Gly Ile Leu Ser Gly Ile Gln Thr Asn Val Ile Asn
290 295 300
Gly Lys Pro Gln Phe Ser Ala Ala Pro Met Thr Leu Leu Tyr Gln Ser
305 310 315 320
Pro Gly Cys Gly Pro Leu Leu Pro Leu Ala Ile Gln Leu Ser Gln Thr
325 330 335
Pro Gly Pro Asn Ser Pro Ile Phe Leu Pro Thr Asp Asp Lys Trp Asp
340 345 350
Trp Leu Leu Ala Lys Thr Trp Val Arg Asn Ala Glu Phe Ser Phe His
355 360 365
Glu Ala Leu Thr His Leu Leu His Ser His Leu Leu Pro Glu Val Phe
370 375 380
Thr Leu Ala Thr Leu Arg Gln Leu Pro His Cys His Pro Leu Phe Lys
385 390 395 400
Leu Leu Ile Pro His Thr Arg Tyr Thr Leu His Ile Asn Thr Leu Ala
405 410 415
Arg Glu Leu Leu Ile Val Pro Gly Gln Val Val Asp Arg Ser Thr Gly
420 425 430
Ile Gly Ile Glu Gly Phe Ser Glu Leu Ile Gln Arg Asn Met Lys Gln
435 440 445
Leu Asn Tyr Ser Leu Leu Cys Leu Pro Glu Asp Ile Arg Thr Arg Gly
450 455 460
Val Glu Asp Ile Pro Gly Tyr Tyr Tyr Arg Asp Asp Gly Met Gln Ile
465 470 475 480
Trp Gly Ala Val Glu Arg Phe Val Ser Glu Ile Ile Gly Ile Tyr Tyr
485 490 495
Pro Ser Asp Glu Ser Val Gln Asp Asp Arg Glu Leu Gln Ala Trp Val
500 505 510
Arg Glu Ile Phe Ser Lys Gly Phe Leu Asn Gln Glu Ser Ser Gly Ile
515 520 525
Pro Ser Ser Leu Glu Thr Arg Glu Ala Leu Val Gln Tyr Val Thr Met
530 535 540
Val Ile Phe Thr Cys Ser Ala Lys His Ala Ala Val Ser Ala Gly Gln
545 550 555 560
Phe Asp Ser Cys Ala Trp Met Pro Asn Leu Pro Pro Ser Met Gln Leu
565 570 575
Pro Pro Pro Thr Ser Lys Gly Leu Ala Thr Cys Glu Gly Phe Ile Ala
580 585 590
Thr Leu Pro Pro Val Asn Ala Thr Cys Asp Val Ile Leu Ala Leu Trp
595 600 605
Leu Leu Ser Lys Glu Pro Gly Asp Gln Arg Pro Leu Gly Thr Tyr Pro
610 615 620
Asp Glu His Phe Thr Glu Glu Ala Pro Arg Arg Ser Ile Ala Thr Phe
625 630 635 640
Gln Ser Arg Leu Ala Gln Ile Ser Arg Gly Ile Gln Glu Arg Asn Gln
645 650 655
Gly Leu Val Leu Pro Tyr Thr Tyr Leu Asp Pro Pro Leu Ile Glu Asn
660 665 670
Ser Val Ser Ile
675
<210> 32
<211> 685
<212> PRT
<213> 铜绿假单胞菌（Pseudomonas aeruginosa）
<400> 32
Met Lys Arg Arg Ser Val Leu Leu Ser Gly Val Ala Leu Ser Gly Thr
1 5 1015
Ala Leu Ala Asn Asp Ser Ile Phe Phe Ser Pro Leu Lys Tyr Leu Gly
202530
Ala Glu Gln Gln Arg Ser Ile Asp Ala Ser Arg Ser Leu Leu Asp Asn
354045
Leu Ile Pro Pro Ser Leu Pro Gln Tyr Asp Asn Leu Ala Gly Lys Leu
505560
Ala Arg Arg Ala Val Leu Thr Ser Lys Lys Leu Val Tyr Val Trp Thr
65707580
Glu Asn Phe Gly Asn Val Lys Gly Val Pro Met Ala Arg Ser Val Pro
859095
Leu Gly Glu Leu Pro Asn Val Asp Trp Leu Leu Lys Thr Ala Gly Val
100 105 110
Ile Val Glu Leu Ile Val Asn Phe Val Ala Ser Leu Pro Ala Ser Ala
115 120 125
Ala Ala Gln Phe Glu Arg Ile Ala Thr Gly Leu Ser Gly Asp Leu Glu
130 135 140
Ala Ala Arg Gln Val His Glu Ala Leu Leu Glu Glu Ala Lys Asn Asp
145 150 155 160
Pro Ala Ala Ala Gly Ser Leu Leu Leu Arg Phe Thr Glu Leu Gln Thr
165 170 175
Arg Val Ile Ala Ile Leu Thr Arg Val Gly Leu Leu Val Asp Asp Ile
180 185 190
Leu Lys Ser Ala Ser Asn Leu Val Thr Gln Arg Gly Gln Gly Asp Gly
195 200 205
Leu Asn Arg Phe Arg Ala Val Phe Gly Thr Leu Arg Leu Pro Glu Val
210 215 220
Ala Asp Ser Phe Arg Asp Asp Glu Ala Phe Ala Tyr Trp Arg Val Ala
225 230 235 240
Gly Pro Asn Pro Leu Leu Ile Arg Arg Val Asp Ala Leu Pro Ala Asn
245 250 255
Phe Pro Leu Gly Glu Glu Gln Phe Arg Arg Val Met Gly Ala Asp Asp
260 265 270
Ser Leu Leu Glu Ala Ala Ala Ser Arg Arg Leu Tyr Leu Leu Asp Tyr
275 280 285
Ala Glu Leu Gly Lys Leu Ala Pro Ser Gly Ala Val Asp Lys Leu Leu
290 295 300
Thr Gly Thr Gly Phe Ala Tyr Ala Pro Ile Ala Leu Phe Ala Leu Gly
305 310 315 320
Lys Asp Arg Ala Arg Leu Leu Pro Val Ala Ile Gln Cys Gly Gln Asp
325 330 335
Pro Ala Thr His Pro Met Phe Val Arg Pro Ala Glu Ser Glu Ser Asp
340 345 350
Leu Tyr Trp Gly Trp Gln Met Ala Lys Thr Val Val Gln Val Ala Glu
355 360 365
Glu Asn Tyr His Glu Met Phe Val His Leu Ala Gln Thr His Leu Val
370 375 380
Ser Glu Ala Phe Cys Leu Ala Thr Gln Arg Thr Leu Ala Pro Ser His
385 390 395 400
Pro Leu His Val Leu Leu Ala Pro His Phe Glu Gly Thr Leu Phe Ile
405 410 415
Asn Glu Gly Ala Ala Arg Ile Leu Leu Pro Ser Ala Gly Phe Ile Asp
420 425 430
Val Met Phe Ala Ala Pro Ile Gln Asp Thr Gln Ala Thr Ala Gly Gly
435 440 445
Asn Arg Leu Gly Phe Asp Phe Tyr Arg Gly Met Leu Pro Glu Ser Leu
450 455 460
Lys Ala Arg Asn Val Asp Asp Pro Leu Ala Leu Pro Asp Tyr Pro Tyr
465 470 475 480
Arg Asp Asp Gly Leu Leu Val Trp Asn Ala Ile Arg Gln Trp Ala Ala
485 490 495
Asp Tyr Val Ala Val Tyr Tyr Ala Ser Asp Gly Asp Val Thr Ala Asp
500 505 510
Val Glu Leu Ala Ala Trp Val Gly Glu Val Ile Gly Ser Gly Lys Val
515 520 525
Ala Gly Phe Arg Pro Ile Thr Gly Arg Ser Gln Leu Val Glu Val Leu
530 535 540
Thr Met Val Ile Phe Thr Ala Ser Ala Gln His Ala Ala Val Asn Phe
545 550 555 560
Pro Gln Pro Ser Met Met Thr Tyr Ala Pro Ala Ile Cys Ala Met Ser
565 570 575
Ala Ala Pro Ala Pro Asp Ser Pro Ser Gly Lys Ser Glu Ala Asp Trp
580 585 590
Leu Lys Met Met Pro Pro Thr Leu Val Ala Leu Glu Lys Val Asn Ile
595 600 605
Tyr His Leu Leu Gly Ser Val Tyr His Gly Arg Leu Gly Asp Tyr Arg
610 615 620
Gln Thr Gly Phe Pro Tyr Ala Pro Val Phe Ser Asp Arg Arg Val Thr
625 630 635 640
Ala Ser Gly Gly Pro Leu Glu Arg Phe Gln Ala Arg Leu Lys Glu Val
645 650 655
Glu Ala Thr Ile Arg Thr Arg Asn Gln Ala Arg Arg Arg Pro Tyr Glu
660 665 670
Tyr Leu Leu Pro Ser Arg Ile Pro Ala Ser Thr Asn Ile
675 680 685
<210> 33
<211> 569
<212> PRT
<213> 蓝丝菌属（Cyanothece sp.）
<400> 33
Met Val Gln Pro Ser Leu Pro Gln Asp Asp Thr Pro Asp Gln Gln Glu
1 5 1015
Gln Arg Asn Arg Ala Ile Ala Gln Gln Arg Glu Ala Tyr Gln Tyr Ser
202530
Glu Thr Ala Gly Ile Leu Leu Ile Lys Thr Leu Pro Gln Ser Glu Met
354045
Phe Ser Leu Lys Tyr Leu Ile Glu Arg Asp Lys Gly Leu Val Ser Leu
505560
Ile Ala Asn Thr Leu Ala Ser Asn Ile Glu Asn Ile Phe Asp Pro Phe
65707580
Asp Lys Leu Glu Asp Phe Glu Glu Met Phe Pro Leu Leu Pro Lys Pro
859095
Leu Val Met Asn Thr Phe Arg Asn Asp Arg Val Phe Ala Arg Gln Arg
100 105 110
Ile Ala Gly Pro Asn Pro Met Val Ile Glu Arg Val Val Asp Lys Leu
115 120 125
Pro Asp Asn Phe Pro Val Thr Asp Ala Met Phe Gln Lys Ile Met Phe
130 135 140
Thr Lys Lys Thr Leu Ala Glu Ala Ile Ala Gln Gly Lys Leu Phe Ile
145 150 155 160
Thr Asn Tyr Lys Gly Leu Ala Glu Leu Ser Pro Gly Arg Tyr Glu Tyr
165 170 175
Gln Lys Asn Gly Thr Leu Val Gln Lys Thr Lys Thr Ile Ala Ala Pro
180 185 190
Leu Val Leu Tyr Ala Trp Lys Pro Glu Gly Phe Gly Asp Tyr Arg Gly
195 200 205
Ser Leu Ala Pro Ile Ala Ile Gln Ile Asn Gln Gln Pro Asp Pro Ile
210 215 220
Thr Asn Pro Ile Tyr Thr Pro Arg Asp Gly Lys His Trp Phe Ile Ala
225 230 235 240
Lys Ile Phe Ala Gln Met Ala Asp Gly Asn Cys His Glu Ala Ile Ser
245 250 255
His Leu Ala Arg Thr His Leu Ile Leu Glu Pro Phe Val Leu Ala Thr
260 265 270
Ala Asn Glu Leu Ala Pro Asn His Pro Leu Ser Val Leu Leu Lys Pro
275 280 285
His Phe Gln Phe Thr Leu Ala Ile Asn Glu Leu Ala Arg Glu Gln Leu
290 295 300
Ile Ser Ala Gly Gly Tyr Ala Asp Asp Leu Leu Ala Gly Thr Leu Glu
305 310 315 320
Ala Ser Ile Ala Val Ile Lys Ala Ala Ile Lys Glu Tyr Met Asp Asn
325 330 335
Phe Thr Glu Phe Ala Leu Pro Arg Glu Leu Ala Arg Arg Gly Val Gly
340 345 350
Ile Gly Asp Val Asp Gln Arg Gly Glu Asn Phe Leu Pro Asp Tyr Pro
355 360 365
Tyr Arg Asp Asp Ala Met Leu Leu Trp Asn Ala Ile Glu Val Tyr Val
370 375 380
Arg Asp Tyr Leu Ser Leu Tyr Tyr Gln Ser Pro Val Gln Ile Arg Gln
385 390 395 400
Asp Thr Glu Leu Gln Asn Trp Val Arg Arg Leu Val Ser Pro Glu Gly
405 410 415
Gly Arg Val Thr Gly Leu Val Ser Asn Gly Glu Leu Asn Thr Ile Glu
420 425 430
Ala Leu Val Ala Ile Ala Thr Gln Val Ile Phe Val Ser Gly Pro Gln
435 440 445
His Ala Ala Val Asn Tyr Pro Gln Tyr Asp Tyr Met Ala Phe Ile Pro
450 455 460
Asn Met Pro Leu Ala Thr Tyr Ala Thr Pro Pro Asn Lys Glu Ser Asn
465 470 475 480
Ile Ser Glu Ala Thr Ile Leu Asn Ile Leu Pro Pro Gln Lys Leu Ala
485 490 495
Ala Arg Gln Leu Glu Leu Met Arg Thr Leu Cys Val Phe Tyr Pro Asn
500 505 510
Arg Leu Gly Tyr Pro Asp Thr Glu Phe Val Asp Val Arg Ala Gln Gln
515 520 525
Val Leu His Gln Phe Gln Glu Arg Leu Gln Glu Ile Glu Gln Arg Ile
530 535 540
Val Leu Cys Asn Glu Lys Arg Leu Glu Pro Tyr Thr Tyr Leu Leu Pro
545 550 555 560
Ser Asn Val Pro Asn Ser Thr Ser Ile
565
<210> 34
<211> 630
<212> PRT
<213> 点形念珠藻（Nostoc punctiforme）
<400> 34
Met Thr Ala Leu Ser Pro Asp His Ser Ile Ser Ser Ser Ser Thr His
1 5 1015
Thr Leu Asp Ile Ala Arg Gln Glu Tyr Gln Tyr Asn Tyr Asn His Ile
202530
Pro Ser Ile Ala Met Val Asp Arg Leu Ser Ile Thr Glu Glu Phe Thr
354045
Thr Asn Trp Tyr Phe Leu Leu Ala Gln Gln Leu Arg Val Leu Phe Ile
505560
Asn Thr Leu Ile Val Asn Arg Gly Asn Gln Asp Ser Lys Ser Ile Arg
65707580
Asp Asp Val Glu Arg Phe Ile Leu Glu Ala Leu Leu Lys Gly Ala Val
859095
Pro Ala Arg Ile Ser Ile Leu Ala Arg Ile Leu Gln Ile Ile Pro Gln
100 105 110
Leu Leu Leu Lys Glu Ile Ser Lys Asp Phe Arg Glu Leu Asp Asp Leu
115 120 125
Phe His Ser Ile Leu Lys Glu Asn Gly Leu Ala Ile Leu Arg Asp Ala
130 135 140
Leu Lys Arg Ile Ile Thr Leu Leu Tyr Glu Gly Gln Pro Thr Gly His
145 150 155 160
Ala Thr Ser Leu Lys Asp Tyr Glu Asn Leu Phe Pro Val Ile Ser Leu
165 170 175
Pro Ala Ile Ala Lys Thr Tyr Gln Glu Asp Glu Val Phe Ala Tyr Met
180 185 190
Arg Val Ala Gly Tyr Asn Pro Val Thr Ile Lys Arg Val Thr Thr Leu
195 200 205
Ser Asp Arg Phe Pro Val Thr Asp Glu His Tyr Gln Ala Val Met Gly
210 215 220
Thr Asp Asp Ser Leu Ala Ala Ala Gly Ile Glu Gly Arg Leu Tyr Leu
225 230 235 240
Ala Asp Tyr Lys Ile Leu Asp Gly Ala Ile Asn Gly Thr Phe Pro His
245 250 255
Glu Gln Lys Tyr Leu Tyr Ala Pro Leu Ala Leu Phe Ala Leu Pro Lys
260 265 270
Gly Ser Asp Pro Thr Arg Leu Leu Arg Pro Val Ala Ile Gln Cys Gly
275 280 285
Gln Thr Pro Gly Pro Asp Tyr Pro Ile Val Thr Pro Asn Ser Gly Lys
290 295 300
Tyr Ala Trp Leu Phe Ala Lys Thr Ile Val Gln Ile Ala Asp Ala Asn
305 310 315 320
Ile His Glu Ala Val Thr His Leu Ala Arg Thr His Leu Leu Val Gly
325 330 335
Val Phe Ala Ile Ala Thr Ala Arg Gln Leu Pro Leu Thr His Pro Leu
340 345 350
Arg Ile Leu Leu Arg Pro His Phe Asp Ser Thr Leu Ala Ile Asn Asp
355 360 365
Ala Ala Gln Arg Ile Leu Ile Ala Pro Gly Gly Gly Val Asp Arg Leu
370 375 380
Leu Ser Ser Ser Ile Asp Asn Ser Arg Val Leu Ala Val Leu Gly Leu
385 390 395 400
Gln Ser Tyr Ser Phe Asn Ser Thr Ile Leu Pro Asn Gln Phe Gln Gln
405 410 415
Arg Gly Val Asp Asp Pro Asn Leu Leu Pro Ile Tyr Pro Tyr Arg Asp
420 425 430
Asp Ala Leu Leu Ile Trp Asn Ala Ile His Gln Trp Val Trp Asp Tyr
435 440 445
Leu Asn Leu Tyr Tyr Thr Thr Asp Glu Asp Ile Gln Lys Asp Arg Ala
450 455 460
Leu Gln Ala Trp Ala Ala Glu Ile Pro Ala Tyr Asp Gly Gly Arg Ile
465 470 475 480
Pro Asp Phe Gly Glu Asp Gly Gly Ile Lys Thr Leu Asn Tyr Leu Ile
485 490 495
Asp Ala Thr Thr Leu Ile Ile Phe Thr Ala Ser Ala Gln His Ala Ala
500 505 510
Val Asn Phe Pro Gln Lys Asp Leu Met Gly Tyr Ala Ala Ala Ile Pro
515 520 525
Leu Ala Gly Tyr Leu Pro Ala Ser Thr Leu Lys Arg Glu Val Thr Glu
530 535 540
Gln Asp Tyr Leu Asn Leu Leu Pro Pro Leu Asp Gln Ala Gln Arg Gln
545 550 555 560
Tyr Asn Leu Leu Ser Leu Leu Gly Ser Val Tyr Tyr Asn Lys Leu Gly
565 570 575
Glu Tyr Glu Gln Gly Tyr Phe Thr Asp Glu Lys Val Lys Pro Leu Leu
580 585 590
Gln Ala Phe Gln Ser His Leu Gln Gln Val Glu Asn Thr Ile Lys Gln
595 600 605
Arg Asn Leu His Arg Pro Pro Tyr Glu Tyr Leu Leu Pro Ser Lys Ile
610 615 620
Pro Gln Ser Ile Asn Ile
625 630
<210> 35
<211> 936
<212> PRT
<213> 多棱大麦（Hordeum vulgare）
<400> 35
Met Leu Thr Ala Thr Lys Pro Leu Val Gly Gly Ala Cys Ala Ala Pro
1 5 1015
Ser Ser Ser Ala Arg Arg Arg Thr Phe Val Val Pro Glu Ala Arg Arg
202530
Lys Pro Gly Asn Gly Arg Arg Thr Ser Val Ser Lys Val Gly Ser Thr
354045
Ser Thr Ser Thr Thr Thr Thr Thr Thr Thr Thr Leu Ser Ala Asp Ser
505560
Asn Gly Ala Ala Val Gly Thr Val Thr Arg Pro Asp Val His Val Gln
65707580
Asp Arg Thr His Ala Thr Glu Met Lys Ala Thr Val Thr Val His Met
859095
Ser Lys Ala Ala Gly Val Arg Asp Phe Leu Tyr Asp Leu Ile Leu Lys
100 105 110
Thr Trp Leu His Val Asp Leu Val Ser Ser Glu Leu Asp Pro Gln Thr
115 120 125
Gly Gln Glu Arg Glu Pro Ile Ser Gly Ala Val Lys His Ser Gly Arg
130 135 140
Val Asp Asp Glu Trp Asp Met Tyr Glu Ala Thr Phe Lys Val Pro Ala
145 150 155 160
Ser Phe Gly Pro Ile Gly Ala Val Gln Val Thr Asn Tyr His His Ser
165 170 175
Glu Met Leu Leu Gly Asp Ile Glu Val Phe Pro Thr Gly Gln Glu Glu
180 185 190
Ser Ala Val Thr Phe His Cys Lys Ser Trp Ile Asp Pro Ser His Cys
195 200 205
Thr Pro Asp Lys Arg Val Phe Phe Pro Ala His Ser Tyr Leu Pro Ser
210 215 220
Gln Thr Pro Lys Gly Val Glu Gly Leu Arg Lys Arg Glu Leu Glu Ile
225 230 235 240
Leu Arg Gly Thr Gly Cys Gly Glu Arg Lys Glu His Asp Arg Ile Tyr
245 250 255
Asp Tyr Asp Val Tyr Asn Asp Leu Gly Asn Pro Asp Asp Asp Asn Asn
260 265 270
Pro Thr Thr Arg Pro Val Leu Gly Gly Lys Glu His Pro Tyr Pro Arg
275 280 285
Arg Cys Arg Thr Gly Arg Pro Arg Ser Lys Lys Asp Pro Phe Ser Glu
290 295 300
Glu Arg Ser His Lys Glu His Ile Tyr Val Pro Arg Asp Glu Ala Phe
305 310 315 320
Thr Glu Arg Lys Met Gly Ala Phe Asp Thr Lys Lys Phe Met Ser Gln
325 330 335
Leu His Ala Leu Thr Thr Gly Leu Lys Thr Ala Lys His Lys Ser Gln
340 345 350
Ser Phe Pro Ser Leu Ser Ala Ile Asp Gln Leu Tyr Asp Asp Asn Phe
355 360 365
Arg Asn Gln Pro Val Gln Pro Glu Gly Gly Lys Leu Arg Phe Val Ile
370 375 380
Asp Leu Leu Glu Thr Glu Leu Leu His Leu Phe Lys Leu Glu Gly Ala
385 390 395 400
Ala Phe Leu Glu Gly Ile Arg Arg Val Phe Lys Phe Glu Thr Pro Glu
405 410 415
Ile His Asp Arg Asp Lys Phe Ala Trp Phe Arg Asp Glu Glu Phe Ala
420 425 430
Arg Gln Thr Ile Ala Gly Met Asn Pro Met Ser Ile Gln Leu Val Thr
435 440 445
Glu Phe Pro Ile Lys Ser Asn Leu Asp Glu Ala Thr Tyr Gly Pro Ala
450 455 460
Asp Ser Leu Ile Thr Lys Glu Val Val Glu Glu Gln Ile Arg Arg Val
465 470 475 480
Met Thr Ala Asp Glu Ala Val Gln Asn Lys Lys Leu Phe Met Leu Asp
485 490 495
Tyr His Asp Leu Leu Leu Pro Tyr Val His Lys Val Arg Lys Leu Asp
500 505 510
Gly Thr Thr Leu Tyr Gly Ser Arg Ala Leu Phe Phe Leu Thr Ala Asp
515 520 525
Gly Thr Leu Arg Pro Ile Ala Ile Glu Leu Thr Arg Pro Lys Ser Lys
530 535 540
Lys Lys Pro Gln Trp Arg Gln Val Phe Thr Pro Gly Cys Asp Gly Ser
545 550 555 560
Val Thr Gly Ser Trp Leu Trp Gln Leu Ala Lys Ala His Ile Leu Ala
565 570 575
His Asp Ala Gly Val His Gln Leu Val Ser His Trp Leu Arg Thr His
580 585 590
Ala Cys Thr Glu Pro Tyr Ile Ile Ala Ala Asn Arg Gln Leu Ser Gln
595 600 605
Met His Pro Val Tyr Arg Leu Leu His Pro His Phe Arg Phe Thr Met
610 615 620
Glu Ile Asn Ala Gln Ala Arg Ala Met Leu Ile Asn Ala Gly Gly Ile
625 630 635 640
Ile Glu Gly Ser Phe Val Pro Gly Glu Tyr Ser Leu Glu Leu Ser Ser
645 650 655
Val Ala Tyr Asp Gln Gln Trp Arg Phe Asp Met Glu Ala Leu Pro Glu
660 665 670
Asp Leu Ile Arg Arg Gly Met Ala Val Arg Asn Pro Asn Gly Glu Leu
675 680 685
Glu Leu Ala Ile Glu Asp Tyr Pro Tyr Ala Asn Asp Gly Leu Leu Val
690 695 700
Trp Asp Ala Ile Lys Gln Trp Ala Leu Thr Tyr Val Gln His Tyr Tyr
705 710 715 720
Pro Cys Ala Ala Asp Ile Val Asp Asp Glu Glu Leu Gln Ala Trp Trp
725 730 735
Thr Glu Val Arg Thr Lys Gly His Ala Asp Lys Gln Asp Glu Pro Trp
740 745 750
Trp Pro Glu Leu Asp Ser His Glu Asn Leu Ala Gln Thr Leu Ala Thr
755 760 765
Ile Met Trp Val Thr Ser Gly His His Ala Ala Val Asn Phe Gly Gln
770 775 780
Tyr Pro Met Ala Gly Tyr Ile Pro Asn Arg Pro Thr Met Ala Arg Arg
785 790 795 800
Asn Met Pro Thr Glu Ile Gly Gly Asp Asp Met Arg Asp Phe Val Glu
805 810 815
Ala Pro Glu Lys Val Leu Leu Asp Thr Phe Pro Ser Gln Tyr Gln Ser
820 825 830
Ala Ile Val Leu Ala Ile Leu Asp Leu Leu Ser Thr His Ser Ser Asp
835 840 845
Glu Glu Tyr Met Gly Thr His Glu Glu Pro Ala Trp Thr Lys Asp Gly
850 855 860
Val Ile Asn Gln Ala Phe Glu Glu Phe Lys Glu Ser Thr Arg Lys Ile
865 870 875 880
Val Glu Gln Val Asp Glu Trp Asn Asn Asp Pro Asp Arg Lys Asn Arg
885 890 895
His Gly Ala Gly Met Val Pro Tyr Val Leu Leu Arg Pro Ser Asp Gly
900 905 910
Asp Pro Thr Asp Gly Asp Pro Thr Asp Glu Lys Met Val Met Glu Met
915 920 925
Gly Ile Pro Asn Ser Ile Ser Ile
930 935
<210> 36
<211> 639
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 36
Met Lys Val Ile Thr Ser Leu Ile Ser Ser Ile Leu Leu Lys Phe Ile
1 5 1015
His Lys Asp Phe His Glu Ile Tyr Ala Arg Met Ser Leu Leu Asp Arg
202530
Phe Leu Leu Leu Ile Val His Gly Val Asp Lys Met Val Pro Trp His
354045
Lys Leu Pro Val Phe Leu Gly Leu Thr Tyr Leu Glu Val Arg Arg His
505560
Leu His Gln Gln Tyr Asn Leu Leu Asn Val Gly Gln Thr Pro Thr Gly
65707580
Ile Arg Phe Asp Pro Ala Asn Tyr Pro Tyr Arg Thr Ala Asp Gly Lys
859095
Phe Asn Asp Pro Phe Asn Glu Gly Val Gly Ser Gln Asn Ser Phe Phe
100 105 110
Gly Arg Asn Cys Pro Pro Val Asp Gln Lys Ser Lys Leu Arg Arg Pro
115 120 125
Asp Pro Met Val Val Ala Thr Lys Leu Leu Gly Arg Lys Lys Phe Ile
130 135 140
Asp Thr Gly Lys Gln Phe Asn Met Ile Ala Ala Ser Trp Ile Gln Phe
145 150 155 160
Met Ile His Asp Trp Ile Asp His Leu Glu Asp Thr His Gln Ile Glu
165 170 175
Leu Val Ala Pro Lys Glu Val Ala Ser Lys Cys Pro Leu Ser Ser Phe
180 185 190
Arg Phe Leu Lys Thr Lys Glu Val Pro Thr Gly Phe Phe Glu Ile Lys
195 200 205
Thr Gly Ser Gln Asn Ile Arg Thr Pro Trp Trp Asp Ser Ser Val Ile
210 215 220
Tyr Gly Ser Asn Ser Lys Thr Leu Asp Arg Val Arg Thr Tyr Lys Asp
225 230 235 240
Gly Lys Leu Lys Ile Ser Glu Glu Thr Gly Leu Leu Leu His Asp Glu
245 250 255
Asp Gly Leu Ala Ile Ser Gly Asp Ile Arg Asn Ser Trp Ala Gly Val
260 265 270
Ser Ala Leu Gln Ala Leu Phe Ile Lys Glu His Asn Ala Val Cys Asp
275 280 285
Ala Leu Lys Asp Glu Asp Asp Asp Leu Glu Asp Glu Asp Leu Tyr Arg
290 295 300
Tyr Ala Arg Leu Val Thr Ser Ala Val Val Ala Lys Ile His Thr Ile
305 310 315 320
Asp Trp Thr Val Gln Leu Leu Lys Thr Asp Thr Leu Leu Ala Gly Met
325 330 335
Arg Ala Asn Trp Tyr Gly Leu Leu Gly Lys Lys Phe Lys Asp Ser Phe
340 345 350
Gly His Ala Gly Ser Ser Ile Leu Gly Gly Val Val Gly Met Lys Lys
355 360 365
Pro Gln Asn His Gly Val Pro Tyr Ser Leu Thr Glu Asp Phe Thr Ser
370 375 380
Val Tyr Arg Met His Ser Leu Leu Pro Asp Gln Leu His Ile Leu Asp
385 390 395 400
Ile Asp Asp Val Pro Gly Thr Asn Lys Ser Leu Pro Leu Ile Gln Glu
405 410 415
Ile Ser Met Arg Asp Leu Ile Gly Arg Lys Gly Glu Glu Thr Met Ser
420 425 430
His Ile Gly Phe Thr Lys Leu Met Val Ser Met Gly His Gln Ala Ser
435 440 445
Gly Ala Leu Glu Leu Met Asn Tyr Pro Met Trp Leu Arg Asp Ile Val
450 455 460
Pro His Asp Pro Asn Gly Gln Ala Arg Pro Asp His Val Asp Leu Ala
465 470 475 480
Ala Leu Glu Ile Tyr Arg Asp Arg Glu Arg Ser Val Pro Arg Tyr Asn
485 490 495
Glu Phe Arg Arg Ser Met Phe Met Ile Pro Ile Thr Lys Trp Glu Asp
500 505 510
Leu Thr Glu Asp Glu Glu Ala Ile Glu Val Leu Asp Asp Val Tyr Asp
515 520 525
Gly Asp Val Glu Glu Leu Asp Leu Leu Val Gly Leu Met Ala Glu Lys
530 535 540
Lys Ile Lys Gly Phe Ala Ile Ser Glu Thr Ala Phe Tyr Ile Phe Leu
545 550 555 560
Ile Met Ala Thr Arg Arg Leu Glu Ala Asp Arg Phe Phe Thr Ser Asp
565 570 575
Phe Asn Glu Thr Ile Tyr Thr Lys Lys Gly Leu Glu Trp Val Asn Thr
580 585 590
Thr Glu Ser Leu Lys Asp Val Ile Asp Arg His Tyr Pro Asp Met Thr
595 600 605
Asp Lys Trp Met Asn Ser Glu Ser Ala Phe Ser Val Trp Asp Ser Pro
610 615 620
Pro Leu Thr Lys Asn Pro Ile Pro Leu Tyr Leu Arg Ile Pro Ser
625 630 635
<210> 37
<211> 631
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 37
Met Gly Phe Ser Pro Ser Ser Ser Trp Phe Leu His Pro Gln Leu His
1 5 1015
His Val Val Ser Lys Met Ser Tyr Phe Asp Ala Phe Leu Phe Tyr Ile
202530
Val His Leu Val Asp Lys Leu Gly Leu Trp His Arg Phe Pro Val Leu
354045
Leu Gly Val Ala Tyr Leu Gly Leu Arg Arg His Leu His Gln Arg Tyr
505560
Asn Leu Val His Val Gly Pro Ile Asn Gly Gln Gly Tyr Asp Thr Asp
65707580
Glu Phe Cys Tyr Arg Thr Ala Asp Gly Lys Cys Asn His Pro Ser Asp
859095
Asn Thr Ile Gly Ser Gln Gly Ser Phe Ile Gly Arg Asn Met Pro Pro
100 105 110
Ser Thr Ser Gln Tyr Gly Ile Leu Asp Pro His Pro Ser Val Val Ala
115 120 125
Thr Lys Leu Leu Ala Arg Lys Arg Phe Ile Asp Asn Gly Asp Gln Phe
130 135 140
Asn Val Ile Ala Cys Ser Trp Ile Gln Phe Met Ile His Asp Trp Val
145 150 155 160
Asp His Leu Glu Asp Thr His Gln Ile Glu Leu Glu Ala Pro Glu Glu
165 170 175
Val Ala Ser Gly Cys Pro Leu Lys Ser Phe Lys Phe Leu Arg Thr Lys
180 185 190
Lys Val Pro Thr Asp Asp His His Lys Ser Gly Ala Val Asn Thr Arg
195 200 205
Thr Pro Trp Trp Asp Gly Ser Val Ile Tyr Gly Asn Asp Glu Thr Gly
210 215 220
Met Arg Arg Val Arg Val Phe Lys Asp Gly Lys Leu Lys Ile Ser Gly
225 230 235 240
Asp Gly Leu Leu Glu Arg Asp Glu Arg Gly Val Pro Ile Ser Gly Asp
245 250 255
Ile Arg Asn Ser Trp Ser Gly Phe Ser Leu Leu Gln Ala Leu Phe Val
260 265 270
Lys Glu His Asn Ser Val Cys Asp Met Leu Lys Glu Arg Tyr Pro Asp
275 280 285
Phe Asp Asp Glu Lys Leu Tyr Arg Thr Ala Arg Leu Val Thr Ala Ala
290 295 300
Val Ile Ala Lys Val His Thr Ile Asp Trp Thr Ile Glu Leu Leu Lys
305 310 315 320
Thr Asp Thr Leu Thr Ala Gly Met Arg Ile Asn Trp Tyr Gly Phe Phe
325 330 335
Gly Lys Lys Val Lys Asp Met Val Gly Ala Arg Phe Gly Pro Leu Phe
340 345 350
Ser Gly Leu Val Gly Leu Lys Lys Pro Asn Asp His Gly Val Pro Tyr
355 360 365
Ser Leu Thr Glu Glu Phe Val Ser Val Tyr Arg Met His Cys Leu Leu
370 375 380
Pro Glu Thr Leu Ile Leu Arg Asp Met Asn Ser Glu Asn Val Asp Lys
385 390 395 400
Glu Asn Pro Ala Ile Glu Arg Glu Ile Pro Met Thr Glu Leu Ile Gly
405 410 415
Lys Lys Ala Gly Glu Lys Ala Ser Lys Leu Gly Phe Glu Gln Leu Leu
420 425 430
Val Ser Met Gly His Gln Ser Cys Gly Ala Leu Thr Leu Trp Asn Tyr
435 440 445
Pro Asn Trp Met Arg Asn Leu Val Ala Gln Asp Ile Asp Gly Glu Asp
450 455 460
Arg Pro His Leu Ile Asp Met Ala Ala Leu Glu Ile Tyr Arg Asp Arg
465 470 475 480
Glu Arg Gly Val Pro Arg Tyr Asn Glu Phe Arg Lys Asn Leu Leu Met
485 490 495
Ser Pro Ile Ser Lys Trp Glu Glu Leu Thr Asp Asp Glu Glu Ala Ile
500 505 510
Lys Val Leu Arg Glu Val Tyr Glu Asp Asp Ile Glu Lys Leu Asp Leu
515 520 525
Asn Val Gly Leu His Ala Glu Lys Lys Ile Lys Gly Phe Ala Ile Ser
530 535 540
Glu Thr Ala Phe Phe Ile Phe Leu Leu Val Ala Ser Arg Arg Leu Glu
545 550 555 560
Ala Asp Arg Phe Phe Thr Thr Asn Phe Asn Glu Lys Thr Tyr Thr Lys
565 570 575
Glu Gly Leu Glu Trp Val Asn Thr Thr Glu Thr Leu Lys Asp Val Ile
580 585 590
Asp Arg His Phe Pro Arg Leu Thr Asp Gln Trp Met Arg Cys Ser Ser
595 600 605
Ala Phe Ser Val Trp Gly Ser Asp Pro Asn Pro Lys Asn Trp Val Pro
610 615 620
Leu Tyr Leu Arg Ser Ala Pro
625 630
<210> 38
<211> 613
<212> PRT
<213> 禾谷镰孢菌（Fusarium graminearum）
<400> 38
Met Ala Ser Val Thr Ser Ile Ile Phe Val Ala Leu Val Ala Gly Val
1 5 1015
Leu Tyr Ala Ala Arg Met Ser Leu Val Pro Ile Leu Lys Ser Leu Tyr
202530
Ile Arg Leu Trp Lys Gly Val Asn His Phe Ile Glu Trp His Lys Leu
354045
Pro Thr Trp Phe Ala Val Phe Asn Leu Leu Ala Leu Arg Tyr Glu Leu
505560
Arg Glu Gly Asn Leu His Asp Thr Ser Pro Asn Ala Glu Phe Gln Gly
65707580
Thr Asp Lys Cys Pro Met Ser Asp Ser Lys Phe Val Ser Ser Arg Asp
859095
Ser Asp Gly Leu Tyr Asn Asp Leu Lys Gln Pro Lys Met Gly Cys Ala
100 105 110
Gly Met Arg Phe Gly Arg Asn Val Pro Arg Lys Tyr Thr Lys Pro Pro
115 120 125
Thr Glu Gln Glu Leu Leu Thr Pro Asn Pro Arg Val Ile Ser Glu Lys
130 135 140
Ile Leu Ala Arg Pro Glu Gly Gln Phe Lys Pro Ala Glu Ile Val Asn
145 150 155 160
Leu Leu Ala Ala Ala Trp Ile Gln Phe Gln Val His Asp Trp Ala Gln
165 170 175
His Phe Leu Val Thr Asn Gly Asp Lys Asp Ile Asp Ile Pro Leu His
180 185 190
Asn Lys Asp Lys Trp Thr Glu Gln Ser Met Lys Ile Pro Arg Thr Lys
195 200 205
Lys Ala Asp Ile Leu Ser Lys Gln Asp Ala Glu Thr Pro Ala Tyr Asp
210 215 220
Asn Glu Asn Thr His Trp Trp Asp Ala Ser Gln Ile Tyr Gly Ser Ser
225 230 235 240
Glu Ala Glu Thr Gln Ala Leu Arg Ala Lys Cys His Lys Ser Lys Pro
245 250 255
Gly Gln Leu Glu Leu His Leu Ser Asn Pro Ser Trp Ser Ser Asp His
260 265 270
Ile Phe Asp Thr Ala Arg Leu Ile Asn Cys Ala Leu Met Ala Lys Ile
275 280 285
His Thr Val Glu Trp Thr Pro Gly Ile Leu Gln His Pro Ala Leu Gln
290 295 300
Ile Gly Met Asn Ala Asn Trp Trp Gly Leu Leu Gly Asp Lys Leu Trp
305 310 315 320
His Ala Phe Gly Arg Val Phe Asp Asn Lys Ser Glu Val Ile Ser Gly
325 330 335
Ile Pro Gly Ser Gly Val Asp His Asp Lys Ala Pro Tyr Cys Leu Thr
340 345 350
Glu Glu Phe Val Ser Val Tyr Arg Leu His Ser Leu Ile Pro Asp Asn
355 360 365
Val Ala Phe Phe Asn Ile Lys Asp Gly Gln His Glu Gly Thr Leu Pro
370 375 380
Ile Val Asp Val Ser Phe Glu Ser Ala Arg Lys Pro Phe Asp Glu Gly
385 390 395 400
Lys Ser Gly Leu Gly Leu Ser Phe Ala Asp Val Phe Tyr Ser Phe Gly
405 410 415
Val Asn Tyr Pro Gly Ala Ile Arg Ala His Asn Met Pro Asn Phe Leu
420 425 430
Arg Asp Leu Lys Ile Pro Ala Asp Lys Asp Phe Pro Glu Gly Arg His
435 440 445
Leu Asp Leu Gly Thr Ile Asp Ile Leu Arg Asp Arg Glu Arg Gly Val
450 455 460
Pro Arg Tyr Asn Ala Phe Arg Arg Leu Phe His Met Pro Ala Ala Lys
465 470 475 480
Ser Phe Ile Asp Leu Thr Gly Gly Asp Asp Lys Leu Ala Ser Glu Leu
485 490 495
Glu Glu Val Tyr Glu Gly Asp Leu Glu Ala Val Asp Leu Leu Val Gly
500 505 510
Thr Leu Cys Glu Pro Leu Pro Lys Gly Phe Gly Phe Ser Asp Thr Ala
515 520 525
Phe Arg Val Phe Ile Leu Met Ala Thr Arg Arg Ile Lys Ser Asp Arg
530 535 540
Phe Ile Ala Gly Asp Gly Trp Cys Pro Glu Val Tyr Thr Arg Glu Gly
545 550 555 560
Met Asp Trp Val Gln Lys Asn Thr Met Lys Asp Val Leu Cys Arg His
565 570 575
Phe Pro Glu Leu Ala Ala Pro Leu His Asn Val Lys Asn Ala Phe Ala
580 585 590
Pro Trp Thr Lys Leu Gly Gln Thr Ala Ala Tyr Ala Gly Pro Glu Thr
595 600 605
Asn Lys Ala Lys Ser
610
<210> 39
<211> 653
<212> PRT
<213> 轮枝样孢刀菌（Fusarium verticillioides）
<400> 39
Met Ala Ser Val Leu Ser Lys Glu Ser Leu Ala Ile Ile Leu Ser Val
1 5 1015
Val Thr Leu Leu Ile Gly Leu Ser His Phe Asn Met Ile Ser Leu Lys
202530
Ser Ile Phe Lys Ser Val Tyr Ile Arg Leu Trp Lys Leu Val Asn Val
354045
Phe Val His Trp His Lys Leu Pro Thr Trp Leu Gly Val Phe Asn Leu
505560
Leu Ala Leu Arg Tyr Glu Leu Arg Glu Lys Asn Leu His Asp Thr Tyr
65707580
Pro Asn Ala Glu Phe Gln Gly Thr Ala Ala Asp Cys Pro Met Lys Asn
859095
Ser Lys Phe Ile Ala Thr Arg Asn Ser Asp Gly Asp Phe Asn Asp Leu
100 105 110
Ala Gln Pro Lys Met Gly Cys Ala Gly Met Arg Phe Gly Arg Asn Val
115 120 125
Pro Arg Asn His Thr Thr Pro Pro Thr Gln Gln Glu Leu Leu Thr Pro
130 135 140
Asn Pro Arg Leu Ile Ser Glu Lys Ile Leu Ala Arg Pro Glu Gly Gln
145 150 155 160
Phe Lys Pro Ala Glu Ile Val Asn Leu Leu Ala Ala Ala Trp Ile Gln
165 170 175
Phe Gln Val His Asp Trp Ala Gln His Ser Leu Val Thr Asn Gly Asp
180 185 190
Lys Asp Val Glu Ile Leu Leu Asp Lys Ala Asp Arg Trp Ser Glu Arg
195 200 205
Ile Met Lys Ile Pro Arg Thr Lys Lys Asp Asp Pro Leu Ser Gln Gln
210 215 220
Asp Ile Glu Thr Pro Ala Tyr Thr Asn Glu Cys Thr His Trp Trp Asp
225 230 235 240
Ala Ser Gln Ile Tyr Gly Ser Thr Glu Ala Glu Thr Lys Ala Leu Arg
245 250 255
Ala Gln Cys Asp Lys Ser Tyr Pro Gly Gln Leu His Val Thr Arg Glu
260 265 270
Asp Gly Val Gln Phe Leu Pro Arg Ser Asp Asp Gly Ile Pro Lys Thr
275 280 285
Gly Phe Arg Gln Asn Trp Trp Leu Gly Leu Glu Leu Leu His Thr Leu
290 295 300
Phe Ala Leu Glu His Asn Ala Ile Ala Thr Gln Leu His Leu Ser Asn
305 310 315 320
Pro Ser Trp Ser Ser Asp Gln Ile Phe Asp Thr Ala Arg Leu Ile Asn
325 330 335
Cys Ala Leu Met Ala Lys Ile His Thr Val Glu Trp Thr Pro Gly Ile
340 345 350
Leu Gln His Pro Ala Leu Gln Ile Gly Met Asn Ala Asn Trp Trp Gly
355 360 365
Leu Leu Gly Asp Lys Leu Trp His Val Phe Gly Arg Val Phe Asp Asn
370 375 380
Lys Ser Glu Val Ile Ser Gly Ile Pro Gly Ser Gly Val Asp His Asp
385 390 395 400
Asn Val Pro Tyr Cys Leu Thr Glu Glu Phe Val Ser Val Tyr Arg Leu
405 410 415
His Pro Leu Ile Pro Asp Asn Val Ala Phe Phe Ser Ile Lys Asp Gly
420 425 430
Gln His Lys Gly Thr Leu Pro Ile Lys Glu Val Ala Phe Lys Ser Ala
435 440 445
Arg Lys Pro Phe Asp Glu Asp Lys Ser Gly Leu Gly Leu Ser Phe Ala
450 455 460
Asp Val Phe Tyr Ser Phe Gly Val Asn Tyr Pro Gly Ala Ile Arg Ala
465 470 475 480
His Asn Met Pro Asn Phe Leu Arg Asp Leu Asn Ile Pro Gly Asp Lys
485 490 495
Asp Phe Pro His Gly Arg His Leu Asp Leu Gly Thr Ile Asp Ile Leu
500 505 510
Arg Asp Arg Glu Arg Gly Val Pro Arg Tyr Asn Ala Phe Arg Arg Leu
515 520 525
Phe His Met Ala Pro Ala Lys Thr Phe Ile Asp Leu Thr Gly Gly Asp
530 535 540
Ser Lys Leu Ala Ala Glu Leu Glu Glu Val Tyr Asp Gly Asp Leu Glu
545 550 555 560
Ala Val Asp Leu Leu Val Gly Thr Leu Ser Glu Pro Leu Pro Lys Gly
565 570 575
Phe Gly Phe Ser Asp Thr Ala Phe Arg Val Phe Ile Leu Met Ala Thr
580 585 590
Arg Arg Ile Lys Ser Asp Arg Phe Leu Ala Gly Asp Gly Trp Cys Pro
595 600 605
Glu Val Tyr Thr Arg Glu Gly Ile Asn Trp Val Gln Asn Asn Thr Met
610 615 620
Lys Asp Val Leu Cys Arg His Phe Pro Glu Leu Ala Ala Thr Leu His
625 630 635 640
Asn Val Lys Asn Leu Ser Val Ser Phe Ile Leu Ser Leu
645 650
<210> 40
<211> 668
<212> PRT
<213> 尖孢镰孢菌（Fusarium oxysporum）
<400> 40
Met Ala Ser Val Leu Ser Lys Glu Ser Leu Ala Ile Ser Leu Ser Ile
1 5 1015
Val Thr Leu Leu Ile Gly Leu Ser His Phe Asn Met Ile Ser Leu Lys
202530
Pro Ile Phe Lys Ser Val Tyr Ile Arg Leu Trp Lys Phe Val Asn Ile
354045
Phe Val His Trp His Lys Leu Pro Thr Trp Leu Gly Val Phe Asn Leu
505560
Leu Ala Leu Arg Tyr Glu Leu Arg Glu Lys Asn Leu His Asp Thr Tyr
65707580
Pro Asn Ala Glu Phe Gln Gly Thr Thr Ala Asp Cys Pro Met Lys Asn
859095
Ser Lys Phe Ile Ala Asn Arg Asn Ser Asp Gly Asp Phe Asn Asp Leu
100 105 110
Ala Gln Pro Lys Met Gly Cys Ala Gly Met Arg Phe Gly Arg Asn Val
115 120 125
Pro Arg Lys Tyr Thr Thr Pro Pro Thr Gln Gln Glu Leu Leu Thr Pro
130 135 140
Asn Pro Arg Ile Ile Ser Glu Lys Ile Leu Ala Arg Pro Glu Gly Gln
145 150 155 160
Phe Lys Pro Ala Glu Ile Val Asn Leu Leu Ala Ala Ala Trp Ile Gln
165 170 175
Phe Gln Val His Asp Trp Ala Gln His Phe Leu Val Thr Asn Gly Asp
180 185 190
Lys Asp Val Glu Ile Pro Leu Asp Lys Ala Asp Arg Trp Ser Glu Arg
195 200 205
Ile Met Lys Ile Pro Arg Thr Lys Lys Asp Asp Ala Leu Ser Gln Gln
210 215 220
Asp Ile Glu Thr Pro Ala Tyr Thr Asn Glu Cys Thr His Trp Trp Asp
225 230 235 240
Ala Ser Gln Ile Tyr Gly Ser Thr Glu Ala Glu Thr Lys Ala Leu Arg
245 250 255
Ala Gln Cys Asp Lys Ser Tyr Pro Gly Gln Leu His Val Thr Arg Glu
260 265 270
Asp Gly Val Gln Phe Leu Pro Arg Ser Asp Asp Gly Ile Pro Lys Thr
275 280 285
Gly Phe Arg Gln Asn Trp Trp Leu Gly Leu Glu Leu Leu His Thr Leu
290 295 300
Phe Ala Leu Glu His Asn Ala Ile Ala Thr Gln Leu His Leu Ser Asn
305 310 315 320
Pro Ser Trp Ser Ser Asp Gln Ile Phe Asp Thr Ala Arg Leu Ile Asn
325 330 335
Cys Ala Leu Met Ala Lys Ile His Thr Val Glu Trp Thr Pro Gly Ile
340 345 350
Leu Gln His Pro Ala Leu Gln Ile Gly Met Asn Ala Asn Trp Trp Gly
355 360 365
Leu Leu Gly Asp Lys Leu Trp His Val Phe Gly Arg Val Phe Asp Asn
370 375 380
Lys Ser Glu Val Ile Ser Gly Ile Pro Gly Ser Gly Val Asp His Asp
385 390 395 400
Asn Val Pro Tyr Cys Leu Thr Glu Glu Phe Val Ser Val Tyr Arg Leu
405 410 415
His Pro Leu Ile Pro Asp Asn Val Ala Phe Phe Ser Ile Lys Asp Gly
420 425 430
Gln His Lys Gly Thr Leu Pro Ile Lys Asp Val Ala Phe Glu Ser Ala
435 440 445
Arg Lys Pro Phe Asp Glu Asp Lys Ser Gly Leu Gly Leu Ser Phe Ala
450 455 460
Asp Val Phe Tyr Ser Phe Gly Val Asn Tyr Pro Gly Ala Ile Arg Ala
465 470 475 480
His Asn Met Pro Asn Phe Leu Arg Asp Leu Asn Ile Pro Gly Asp Lys
485 490 495
Asp Phe Pro Gln Gly Arg His Leu Asp Leu Gly Thr Ile Asp Ile Leu
500 505 510
Arg Asp Arg Glu Arg Gly Val Pro Arg Tyr Asn Ala Phe Arg Arg Leu
515 520 525
Phe His Met Ala Pro Ala Lys Ser Phe Leu Asp Leu Thr Gly Gly Asp
530 535 540
Ala Lys Leu Ala Ala Glu Leu Glu Asp Val Tyr Asp Gly Asp Leu Glu
545 550 555 560
Ala Val Asp Leu Leu Val Gly Thr Leu Ser Glu Pro Leu Pro Lys Gly
565 570 575
Phe Gly Phe Ser Asp Thr Ala Phe Arg Val Phe Ile Leu Met Ala Thr
580 585 590
Arg Arg Ile Lys Ser Asp Arg Phe Leu Ala Gly Asp Gly Trp Cys Pro
595 600 605
Glu Val Tyr Thr Arg Glu Gly Ile Asn Trp Val Gln Asn Asn Thr Met
610 615 620
Lys Asp Val Leu Cys Arg His Phe Pro Glu Leu Ala Ala Thr Leu His
625 630 635 640
Asn Val Lys Asn Ala Phe Ala Pro Trp Thr Lys Ile Gly Gln Thr Glu
645 650 655
Ala Tyr Ala Gly Pro Glu Thr Asn Lys Ala Lys Asn
660 665
<210> 41
<211> 618
<212> PRT
<213> Oryza sative
<400> 41
Met Gly Ser Gly Leu Phe Lys Pro Arg Val His Pro Asp Leu Arg Asp
1 5 1015
Val Phe Ser Lys Met Ser Phe Phe Asp Lys Ile Gly Phe Leu Phe Ile
202530
His Ala Phe Asp Lys Arg Asn Leu Trp His Lys Val Pro Val Pro Ile
354045
Gly Leu Leu Tyr Leu Asn Thr Arg Arg Thr Leu Leu Glu Lys Tyr Asn
505560
Leu Leu Ala Val Gly Arg Ser Ser His Gly Ala Leu Phe Asp Pro Lys
65707580
Glu Phe Leu Tyr Arg Thr Glu Asp Gly Lys Tyr Asn Asp Pro His Asn
859095
Ala Glu Ala Gly Ser Gln Asn Thr Phe Phe Gly Arg Asn Met Glu Pro
100 105 110
Val Asp Gln Gln Asp Glu Leu Met Ser Pro Asp Pro Phe Val Val Ala
115 120 125
Thr Lys Leu Leu Ala Arg Arg Glu Tyr Lys Asp Thr Gly Lys Gln Phe
130 135 140
Asn Ile Leu Ala Ala Ala Trp Ile Gln Phe Met Val His Asp Trp Met
145 150 155 160
Asp His Met Glu Asp Thr Gly Gln Ile Gly Ile Thr Ala Pro Lys Glu
165 170 175
Val Ala Asn Glu Cys Pro Leu Lys Ser Phe Lys Phe His Pro Thr Lys
180 185 190
Glu Leu Pro Thr Asn Ser Asp Gly Ile Lys Ile Gly His Tyr Asn Ile
195 200 205
Arg Thr Ala Trp Trp Asp Gly Ser Ala Val Tyr Gly Asn Asn Glu Glu
210 215 220
Arg Ala Glu Lys Leu Arg Thr Tyr Val Asp Gly Lys Leu Val Ile Gly
225 230 235 240
Asp Asp Gly Leu Leu Leu His Lys Glu Asn Gly Val Ala Leu Ser Gly
245 250 255
Asp Ile Arg Asn Ser Trp Ala Gly Val Ser Ile Leu Gln Ala Leu Phe
260 265 270
Val Lys Glu His Asn Ala Val Cys Asp Ala Ile Lys Glu Glu His Pro
275 280 285
Asn Leu Ser Asp Glu Glu Leu Tyr Arg Tyr Ala Lys Leu Val Thr Ser
290 295 300
Ala Val Ile Ala Lys Val His Thr Ile Asp Trp Thr Val Glu Leu Leu
305 310 315 320
Lys Thr Lys Thr Met Arg Ala Ala Met Arg Ala Asn Trp Tyr Gly Leu
325 330 335
Leu Gly Lys Lys Ile Lys Asp Thr Phe Gly His Ile Gly Gly Pro Ile
340 345 350
Leu Gly Gly Leu Val Gly Leu Lys Lys Pro Asn Asn His Gly Val Pro
355 360 365
Tyr Ser Leu Thr Glu Glu Phe Thr Ser Val Tyr Arg Met His Ser Leu
370 375 380
Ile Pro Ser Thr Leu Lys Leu Arg Asp Pro Thr Gly Gln Pro Asp Ala
385 390 395 400
Asn Asn Ser Pro Pro Cys Leu Glu Asp Ile Asp Ile Gly Glu Met Ile
405 410 415
Gly Leu Lys Gly Glu Glu Gln Leu Ser Lys Ile Gly Phe Glu Lys Gln
420 425 430
Ala Leu Ser Met Gly Tyr Gln Ala Cys Gly Ala Leu Glu Leu Trp Asn
435 440 445
Tyr Pro Ser Phe Phe Arg Asn Leu Ile Pro Gln Asn Leu Asp Gly Thr
450 455 460
Asn Arg Ser Asp Arg Ile Asp Leu Ala Ala Leu Glu Val Tyr Arg Asp
465 470 475 480
Arg Glu Arg Ser Val Pro Arg Tyr Asn Glu Phe Arg Arg Arg Leu Phe
485 490 495
Leu Ile Pro Ile Lys Ser Trp Glu Asp Leu Thr Ser Asp Lys Asp Ala
500 505 510
Ile Glu Thr Ile Arg Ala Ile Tyr Gly Asp Asp Val Glu Lys Leu Asp
515 520 525
Leu Leu Val Gly Leu Met Ala Glu Lys Lys Ile Lys Gly Phe Ala Ile
530 535 540
Ser Glu Thr Ala Phe Asn Ile Phe Ile Leu Met Ala Ser Arg Arg Leu
545 550 555 560
Glu Ala Asp Arg Phe Phe Thr Ser Asn Phe Asn Glu Glu Thr Tyr Thr
565 570 575
Lys Lys Gly Met Gln Trp Val Lys Thr Thr Glu Gly Leu Arg Asp Val
580 585 590
Ile Asn Arg His Tyr Pro Glu Ile Thr Ala Lys Trp Met Lys Ser Ser
595 600 605
Ser Ala Phe Ser Val Trp Asp Ala Asp Tyr
610 615
<210> 42
<211> 599
<212> PRT
<213> 智人（Homo sapiens）
<400> 42
Met Ser Arg Ser Leu Leu Leu Trp Phe Leu Leu Phe Leu Leu Leu Leu
1 5 1015
Pro Pro Leu Pro Val Leu Leu Ala Asp Pro Gly Ala Pro Thr Pro Val
202530
Asn Pro Cys Cys Tyr Tyr Pro Cys Gln His Gln Gly Ile Cys Val Arg
354045
Phe Gly Leu Asp Arg Tyr Gln Cys Asp Cys Thr Arg Thr Gly Tyr Ser
505560
Gly Pro Asn Cys Thr Ile Pro Gly Leu Trp Thr Trp Leu Arg Asn Ser
65707580
Leu Arg Pro Ser Pro Ser Phe Thr His Phe Leu Leu Thr His Gly Arg
859095
Trp Phe Trp Glu Phe Val Asn Ala Thr Phe Ile Arg Glu Met Leu Met
100 105 110
Arg Leu Val Leu Thr Val Arg Ser Asn Leu Ile Pro Ser Pro Pro Thr
115 120 125
Tyr Asn Ser Ala His Asp Tyr Ile Ser Trp Glu Ser Phe Ser Asn Val
130 135 140
Ser Tyr Tyr Thr Arg Ile Leu Pro Ser Val Pro Lys Asp Cys Pro Thr
145 150 155 160
Pro Met Gly Thr Lys Gly Lys Lys Gln Leu Pro Asp Ala Gln Leu Leu
165 170 175
Ala Arg Arg Phe Leu Leu Arg Arg Lys Phe Ile Pro Asp Pro Gln Gly
180 185 190
Thr Asn Leu Met Phe Ala Phe Phe Ala Gln His Phe Thr His Gln Phe
195 200 205
Phe Lys Thr Ser Gly Lys Met Gly Pro Gly Phe Thr Lys Ala Leu Gly
210 215 220
His Gly Val Asp Leu Gly His Ile Tyr Gly Asp Asn Leu Glu Arg Gln
225 230 235 240
Tyr Gln Leu Arg Leu Phe Lys Asp Gly Lys Leu Lys Tyr Gln Val Leu
245 250 255
Asp Gly Glu Met Tyr Pro Pro Ser Val Glu Glu Ala Pro Val Leu Met
260 265 270
His Tyr Pro Arg Gly Ile Pro Pro Gln Ser Gln Met Ala Val Gly Gln
275 280 285
Glu Val Phe Gly Leu Leu Pro Gly Leu Met Leu Tyr Ala Thr Leu Trp
290 295 300
Leu Arg Glu His Asn Arg Val Cys Asp Leu Leu Lys Ala Glu His Pro
305 310 315 320
Thr Trp Gly Asp Glu Gln Leu Phe Gln Thr Thr Arg Leu Ile Leu Ile
325 330 335
Gly Glu Thr Ile Lys Ile Val Ile Glu Glu Tyr Val Gln Gln Leu Ser
340 345 350
Gly Tyr Phe Leu Gln Leu Lys Phe Asp Pro Glu Leu Leu Phe Gly Val
355 360 365
Gln Phe Gln Tyr Arg Asn Arg Ile Ala Met Glu Phe Asn His Leu Tyr
370 375 380
His Trp His Pro Leu Met Pro Asp Ser Phe Lys Val Gly Ser Gln Glu
385 390 395 400
Tyr Ser Tyr Glu Gln Phe Leu Phe Asn Thr Ser Met Leu Val Asp Tyr
405 410 415
Gly Val Glu Ala Leu Val Asp Ala Phe Ser Arg Gln Ile Ala Gly Arg
420 425 430
Ile Gly Gly Gly Arg Asn Met Asp His His Ile Leu His Val Ala Val
435 440 445
Asp Val Ile Arg Glu Ser Arg Glu Met Arg Leu Gln Pro Phe Asn Glu
450 455 460
Tyr Arg Lys Arg Phe Gly Met Lys Pro Tyr Thr Ser Phe Gln Glu Leu
465 470 475 480
Val Gly Glu Lys Glu Met Ala Ala Glu Leu Glu Glu Leu Tyr Gly Asp
485 490 495
Ile Asp Ala Leu Glu Phe Tyr Pro Gly Leu Leu Leu Glu Lys Cys His
500 505 510
Pro Asn Ser Ile Phe Gly Glu Ser Met Ile Glu Ile Gly Ala Pro Phe
515 520 525
Ser Leu Lys Gly Leu Leu Gly Asn Pro Ile Cys Ser Pro Glu Tyr Trp
530 535 540
Lys Pro Ser Thr Phe Gly Gly Glu Val Gly Phe Asn Ile Val Lys Thr
545 550 555 560
Ala Thr Leu Lys Lys Leu Val Cys Leu Asn Thr Lys Thr Cys Pro Tyr
565 570 575
Val Ser Phe Arg Val Pro Asp Ala Ser Gln Asp Asp Gly Pro Ala Val
580 585 590
Glu Arg Pro Ser Thr Glu Leu
595
<210> 43
<211> 624
<212> PRT
<213> 铜绿假单胞菌（Pseudomonas aeruginosa）
<400> 43
Met His Pro Thr Phe Ser Arg Val Leu Leu Ala Ala Ala Leu Ala Ala
1 5 1015
Ala Gly Ser Pro Ala Val Ala Thr Glu Ile Gln Leu Glu Gln Gly Trp
202530
Asn Ala Glu Gln Arg Ala Ser Trp Tyr Asp Ala Ser Leu Gly Ser Arg
354045
Leu Leu Pro Leu Ala Trp Ala Gln Ala Leu Glu Arg Pro Asp Ser Glu
505560
Glu Arg Leu Phe Ser Glu Asp Asn Ala Arg Arg Leu Gly Phe Pro Leu
65707580
Arg Asn Trp Gln Gly Gly Glu Leu Arg Leu Pro Arg Gly Phe Ala Leu
859095
Asp Gln Gln Asp Asp Ser Gln Phe Ser Asp Thr Arg Leu Arg Trp Lys
100 105 110
Ala Arg Gln Ser Ser Ser Glu Pro Trp Val Gly Leu Asn Cys Ala Gly
115 120 125
Cys His Ser Thr Asp Ile Ser Tyr Arg Gly Ser Glu Leu Thr Val Asp
130 135 140
Ala Gly Ala Thr Leu Ala Asn Val Gln Ala Ile Phe Asp Glu Val Leu
145 150 155 160
Ala Ala Leu Arg Arg Thr Ser Asp Asp Gly Asp Lys Phe Ala Arg Phe
165 170 175
Ala Gly Asn Val Leu Gly Ser Glu Asp Ser Pro Ala Asn Arg Glu Leu
180 185 190
Leu Lys Ala Ala Leu Val Lys Arg Ala Ala Leu Ile Asp Thr Leu Leu
195 200 205
Ser Met Ser Ala Thr Asp Leu Gln Pro Gly Pro Gly Arg Leu Asp Ala
210 215 220
Thr Gly Gln Ser Leu Asn Arg Ala Ala Ile Asn Ser Gly Ala Arg His
225 230 235 240
Leu Gln Ala Asn Pro Thr Asp Ala Pro Thr Ser Phe Pro Ala Leu Trp
245 250 255
His Thr Leu Gln Met Asp Lys Leu Gln Ser Ser Gly Phe Val Pro Asn
260 265 270
Val Lys Val Leu Asp Leu Asn Gly Gln Val Phe Asp Leu Gly Tyr Leu
275 280 285
Ala Gly Asp Ile Gly Val Val Gln Gly Asp Tyr Gly Asp Val Val Ser
290 295 300
His Pro Leu Ser Gly Leu Glu Gly Tyr Ile Ser Ser Ile Arg Val Asp
305 310 315 320
Asn Leu Thr Arg Val Glu Gly Leu Ile His Lys Leu Lys Ala Pro Ala
325 330 335
Trp Pro Ser Gln Leu Phe Gly Ala Pro Asp Ser Ala Arg Leu Ala Gln
340 345 350
Gly Lys Arg Leu Tyr Glu Glu Asn Cys Ala Ala Cys His Ala Ser Ile
355 360 365
Gly Arg Asp Asp Leu Gln Thr Pro Ile Lys Val Arg Gln Val Arg Leu
370 375 380
Lys Ala His Gly Asp Asp Ala Pro Ile Gly Thr Asp Pro Trp Met Ala
385 390 395 400
Cys Asn Thr Phe Thr Phe Ser Ser Pro Ser Gly Asn Tyr Phe Gly Leu
405 410 415
Phe Arg Pro Ser Leu Gly Thr Pro Ser Gly Val Gly Ile Val Gly Arg
420 425 430
Thr Ser Lys Ile Ala Asp Met Gln Val Pro Glu Val Phe Gln Ile Met
435 440 445
Leu Gly Lys Lys Gly Gln Leu Ala Asp Gly Ile Ala Glu Ile Ile His
450 455 460
Ala Ile Val Thr Gly Gln Gln Thr Leu Pro Gly Ser Asp Ser Leu Gln
465 470 475 480
Ala Val Pro Ala Gly Gln Leu Leu Leu Ala Gly Ala Ala Pro Ala Asp
485 490 495
Ser Gln Ala Gln Ser Leu Ala Ala Gly Glu Val Pro Thr Asp Lys Ser
500 505 510
Ala Arg Lys Asp Tyr Cys Leu Asn Thr Glu His Pro Phe Leu Gly Tyr
515 520 525
Ile Ala Arg Pro Leu Asn Gly Ile Trp Ala Thr Ala Pro Tyr Leu His
530 535 540
Asn Gly Ser Val Pro Ser Leu Tyr Asp Leu Leu Leu Pro Gln Glu Gln
545 550 555 560
Arg Pro Ala Thr Phe Tyr Thr Gly Ser His Glu Phe Asp Pro Ser Arg
565 570 575
Val Gly Tyr Leu Thr Ala Pro Gly Pro Asp Asn Ala Phe Leu Phe Asp
580 585 590
Thr His Leu Glu Gly Asn Ser Asn Ala Gly His Asp Phe Ala Arg Glu
595 600 605
Tyr Asp Glu Ser Gln Arg Leu Ala Leu Leu Glu Tyr Leu Lys Thr Leu
610 615 620
<210> 44
<211> 624
<212> PRT
<213> 铜绿假单胞菌（Pseudomonas aeruginosa）
<400> 44
Met His Pro Thr Phe Ser Arg Ile Leu Leu Ala Ala Ala Leu Ala Ser
1 5 1015
Ala Gly Ser Pro Ala Val Ala Thr Glu Ile Gln Leu Glu Gln Gly Trp
202530
Asn Ala Glu Gln Arg Ala Ser Trp Tyr Asp Ala Ser Leu Gly Ser Arg
354045
Leu Leu Pro Leu Ala Trp Ala Gln Ala Leu Glu Arg Pro Asp Ser Glu
505560
Glu Arg Leu Phe Ser Glu Asp Asn Ala Arg Arg Leu Gly Phe Pro Leu
65707580
Arg Asn Trp Gln Gly Gly Glu Leu Arg Leu Pro Arg Gly Phe Ala Leu
859095
Asp Gln Gln Asp Asp Ser Gln Phe Ser Asp Thr Arg Leu Arg Trp Lys
100 105 110
Ala Arg Gln Ser Ser Ser Glu Pro Trp Val Gly Leu Asn Cys Ala Gly
115 120 125
Cys His Ser Thr Asp Ile Ser Tyr Arg Gly Ser Glu Leu Thr Val Asp
130 135 140
Ala Gly Ala Thr Leu Ala Asn Val Gln Ala Ile Phe Asp Glu Val Leu
145 150 155 160
Ala Ala Leu Arg Arg Thr Ser Asp Asp Gly Asp Lys Phe Ala Arg Phe
165 170 175
Ala Gly Asn Val Leu Gly Ser Glu Asp Ser Pro Ala Asn Arg Asp Leu
180 185 190
Leu Lys Ala Ala Leu Ala Lys Cys Ala Ala Leu Ile Asp Thr Leu Leu
195 200 205
Ser Met Ser Ala Thr Asp Leu Gln Pro Gly Pro Gly Arg Leu Asp Ala
210 215 220
Thr Gly Gln Ser Leu Asn Arg Ala Ala Ile Asn Ser Gly Ala Arg His
225 230 235 240
Leu Gln Ala Asn Pro Thr Asp Ala Pro Thr Ser Phe Pro Ala Leu Trp
245 250 255
His Thr Leu Gln Met Asp Lys Leu Gln Ser Ser Gly Phe Val Pro Asn
260 265 270
Val Lys Val Leu Asp Leu Asn Gly Gln Val Phe Asp Leu Gly Tyr Leu
275 280 285
Ala Gly Asp Ile Gly Val Val Gln Gly Asp Tyr Gly Asp Val Val Ser
290 295 300
His Pro Leu Ser Gly Leu Glu Gly Tyr Ile Ser Ser Ile Arg Val Asp
305 310 315 320
Asn Leu Thr Arg Val Glu Gly Leu Ile His Lys Leu Lys Ala Pro Ala
325 330 335
Trp Pro Ser Gln Leu Phe Gly Ala Pro Asp Ser Ala Arg Leu Ala Gln
340 345 350
Gly Lys Arg Leu Tyr Glu Glu Asn Cys Ala Ala Cys His Ala Ser Ile
355 360 365
Gly Arg Asp Asp Leu Gln Thr Pro Ile Lys Val Arg Gln Val Arg Leu
370 375 380
Lys Ala His Gly Asp Asp Ala Pro Ile Gly Thr Asp Pro Trp Met Ala
385 390 395 400
Cys Asn Thr Phe Thr Phe Ser Ser Pro Ser Gly Asn Tyr Phe Gly Leu
405 410 415
Phe Arg Pro Ser Leu Gly Thr Pro Ser Gly Val Gly Ile Val Gly Arg
420 425 430
Thr Ser Lys Ile Ala Asp Met Gln Val Pro Glu Val Phe Gln Ile Met
435 440 445
Leu Gly Lys Lys Gly Gln Leu Ala Asp Gly Ile Ala Glu Ile Ile His
450 455 460
Ala Ile Val Thr Gly Gln Gln Thr Leu Pro Gly Ser Asp Ser Leu Gln
465 470 475 480
Ala Leu Pro Thr Gly Gln Leu Leu Leu Ala Gly Gly Asp Pro Ala Gly
485 490 495
Ser Gln Ala Pro Ser Leu Ala Ala Gly Glu Val Pro Ala Asp Lys Ser
500 505 510
Ala Arg Lys Asp Tyr Cys Leu Asn Thr Glu His Pro Phe Leu Gly Tyr
515 520 525
Ile Ala Arg Pro Leu Asn Gly Ile Trp Ala Thr Ala Pro Tyr Leu His
530 535 540
Asn Gly Ser Val Pro Ser Leu Tyr Asp Leu Leu Leu Pro Gln Glu Gln
545 550 555 560
Arg Pro Thr Thr Phe Tyr Thr Gly Ser His Glu Phe Asp Pro Ser Arg
565 570 575
Val Gly Tyr Leu Thr Gly Pro Gly Pro Asp Asn Ala Phe Leu Phe Asp
580 585 590
Thr His Leu Glu Gly Asn Ser Asn Ala Gly His Asp Phe Ala Arg Glu
595 600 605
Tyr Asp Glu Ser Gln Arg Leu Ala Leu Leu Glu Tyr Leu Lys Thr Leu
610 615 620
<210> 45
<211> 711
<212> PRT
<213> 智人（Homo sapiens）
<400> 45
Met Ala Val Tyr Arg Leu Cys Val Thr Thr Gly Pro Tyr Leu Arg Ala
1 5 1015
Gly Thr Leu Asp Asn Ile Ser Val Thr Leu Val Gly Thr Cys Gly Glu
202530
Ser Pro Lys Gln Arg Leu Asp Arg Met Gly Arg Asp Phe Ala Pro Gly
354045
Ser Val Gln Lys Tyr Lys Val Arg Cys Thr Ala Glu Leu Gly Glu Leu
505560
Leu Leu Leu Arg Val His Lys Glu Arg Tyr Ala Phe Phe Arg Lys Asp
65707580
Ser Trp Tyr Cys Ser Arg Ile Cys Val Thr Glu Pro Asp Gly Ser Val
859095
Ser His Phe Pro Cys Tyr Gln Trp Ile Glu Gly Tyr Cys Thr Val Glu
100 105 110
Leu Arg Pro Gly Thr Ala Arg Thr Ile Cys Gln Asp Ser Leu Pro Leu
115 120 125
Leu Leu Asp His Arg Thr Arg Glu Leu Arg Ala Arg Gln Glu Cys Tyr
130 135 140
Arg Trp Lys Ile Tyr Ala Pro Gly Phe Pro Cys Met Val Asp Val Asn
145 150 155 160
Ser Phe Gln Glu Met Glu Ser Asp Lys Lys Phe Ala Leu Thr Lys Thr
165 170 175
Thr Thr Cys Val Asp Gln Gly Asp Ser Ser Gly Asn Arg Tyr Leu Pro
180 185 190
Gly Phe Pro Met Lys Ile Asp Ile Pro Ser Leu Met Tyr Met Glu Pro
195 200 205
Asn Val Arg Tyr Ser Ala Thr Lys Thr Ile Ser Leu Leu Phe Asn Ala
210 215 220
Ile Pro Ala Ser Leu Gly Met Lys Leu Arg Gly Leu Leu Asp Arg Lys
225 230 235 240
Gly Ser Trp Lys Lys Leu Asp Asp Met Gln Asn Ile Phe Trp Cys His
245 250 255
Lys Thr Phe Thr Thr Lys Tyr Val Thr Glu His Trp Cys Glu Asp His
260 265 270
Phe Phe Gly Tyr Gln Tyr Leu Asn Gly Val Asn Pro Val Met Leu His
275 280 285
Cys Ile Ser Ser Leu Pro Ser Lys Leu Pro Val Thr Asn Asp Met Val
290 295 300
Ala Pro Leu Leu Gly Gln Asp Thr Cys Leu Gln Thr Glu Leu Glu Arg
305 310 315 320
Gly Asn Ile Phe Leu Ala Asp Tyr Trp Ile Leu Ala Glu Ala Pro Thr
325 330 335
His Cys Leu Asn Gly Arg Gln Gln Tyr Val Ala Ala Pro Leu Cys Leu
340 345 350
Leu Trp Leu Ser Pro Gln Gly Ala Leu Val Pro Leu Ala Ile Gln Leu
355 360 365
Ser Gln Thr Pro Gly Pro Asp Ser Pro Ile Phe Leu Pro Thr Asp Ser
370 375 380
Glu Trp Asp Trp Leu Leu Ala Lys Thr Trp Val Arg Asn Ser Glu Phe
385 390 395 400
Leu Val His Glu Asn Asn Thr His Phe Leu Cys Thr His Leu Leu Cys
405 410 415
Glu Ala Phe Ala Met Ala Thr Leu Arg Gln Leu Pro Leu Cys His Pro
420 425 430
Ile Tyr Lys Leu Leu Leu Pro His Thr Arg Tyr Thr Leu Gln Val Asn
435 440 445
Thr Ile Ala Arg Ala Thr Leu Leu Asn Pro Glu Gly Leu Val Asp Gln
450 455 460
Val Thr Ser Ile Gly Arg Gln Gly Leu Ile Tyr Leu Met Ser Thr Gly
465 470 475 480
Leu Ala His Phe Thr Tyr Thr Asn Phe Cys Leu Pro Asp Ser Leu Arg
485 490 495
Ala Arg Gly Val Leu Ala Ile Pro Asn Tyr His Tyr Arg Asp Asp Gly
500 505 510
Leu Lys Ile Trp Ala Ala Ile Glu Ser Phe Val Ser Glu Ile Val Gly
515 520 525
Tyr Tyr Tyr Pro Ser Asp Ala Ser Val Gln Gln Asp Ser Glu Leu Gln
530 535 540
Ala Trp Thr Gly Glu Ile Phe Ala Gln Ala Phe Leu Gly Arg Glu Ser
545 550 555 560
Ser Gly Phe Pro Ser Arg Leu Cys Thr Pro Gly Glu Met Val Lys Phe
565 570 575
Leu Thr Ala Ile Ile Phe Asn Cys Ser Ala Gln His Ala Ala Val Asn
580 585 590
Ser Gly Gln His Asp Phe Gly Ala Trp Met Pro Asn Ala Pro Ser Ser
595 600 605
Met Arg Gln Pro Pro Pro Gln Thr Lys Gly Thr Thr Thr Leu Lys Thr
610 615 620
Tyr Leu Asp Thr Leu Pro Glu Val Asn Ile Ser Cys Asn Asn Leu Leu
625 630 635 640
Leu Phe Trp Leu Val Ser Gln Glu Pro Lys Asp Gln Arg Pro Leu Gly
645 650 655
Thr Tyr Pro Asp Glu His Phe Thr Glu Glu Ala Pro Arg Arg Ser Ile
660 665 670
Ala Ala Phe Gln Ser Arg Leu Ala Gln Ile Ser Arg Asp Ile Gln Glu
675 680 685
Arg Asn Gln Gly Leu Ala Leu Pro Tyr Thr Tyr Leu Asp Pro Pro Leu
690 695 700
Ile Glu Asn Ser Val Ser Ile
705 710
<210> 46
<211> 393
<212> PRT
<213> 点形念珠藻（Nostoc punctiforme）
<400> 46
Met Lys Leu Phe Thr Glu Tyr Pro Glu Lys Asp Glu Leu Lys Tyr Cys
1 5 1015
Asp Leu Met Ser Glu Leu Val Lys Lys Asn Met Glu Asn Leu Tyr Gly
202530
Gly Lys Lys Lys Lys Thr Ala Lys Arg Asp Thr His Ser Lys Thr His
354045
Ala Ala Val Gln Gly Thr Leu Glu Ile Phe Asp Phe Asp Glu Ala Ala
505560
Ile Lys Gln Glu Leu Ser Lys Arg Thr Ser Leu Ser Glu Ala Glu Leu
65707580
Ser Ala Ile Ser Leu Lys Gln Gly Leu Phe Ala Thr Pro Lys Gln Tyr
859095
Pro Val Trp Leu Arg Phe Ala Asn Gly Ala Phe Ser Val Lys Asn Asp
100 105 110
Tyr Glu Gly Asp Thr Arg Ser Met Ala Val Lys Ala Ile Gly Val Glu
115 120 125
Gly Glu Arg Leu Ser Gln Ser His Glu Leu Lys Thr Gln Asp Ile Ile
130 135 140
Thr His Asn Thr Glu Phe Phe Phe Val Arg Thr Ile Lys Asp Phe His
145 150 155 160
Ser Phe Phe Leu Thr Val Tyr Arg Ala Gly Leu Phe Pro Leu Phe Lys
165 170 175
Leu Leu Val Leu Phe Trp Leu Lys Leu His Pro Tyr Glu Ser Thr Leu
180 185 190
Leu Gln Thr Ser Phe Lys Arg Phe Pro Lys Ser Leu Leu Lys Glu Arg
195 200 205
Tyr Trp Ser Ala Ser Ala Phe Ser Val Gly Leu Lys Ser Gly Phe Asp
210 215 220
Pro Ser Gln Pro Gly Arg Val Pro Val Glu Tyr Pro Ala Val Ile Lys
225 230 235 240
Tyr Gly Phe Thr Pro Val Ser Ser Gln Pro Pro His Gln Gln Phe Pro
245 250 255
Leu Glu Ser Arg Ser Glu Ser Glu Leu Lys Leu Ala Lys Ala Ser Gly
260 265 270
Ser Glu Asp Asn Tyr Tyr Arg Glu Asp Ile Ile Gln Ala Leu Ala Lys
275 280 285
Pro Asp Ala Glu Tyr Tyr Trp Asp Phe Gln Ile Gln Phe Gln Thr Ser
290 295 300
Pro Glu Met Ser Ile Asp Asp Thr Thr Ile Val Trp Asn Glu Glu Glu
305 310 315 320
Ser Pro Phe Phe Thr Val Gly Arg Leu Thr Ile Lys His Gln Lys Val
325 330 335
Asn Tyr Pro Gln Ala Asn Asp Phe Gly Glu Asn Leu Ser Phe Ser Pro
340 345 350
Gly Asn Gly Leu Ala Val His Arg Pro Val Gly Ala Ile Asn Arg Leu
355 360 365
Arg Ser Ile Val Tyr Pro Ile Val Ala Asn Asp Arg His Gln Lys Arg
370 375 380
Gly Val Lys Tyr Gln Glu Pro Thr Val
385 390
<210> 47
<211> 1143
<212> PRT
<213> 土曲霉（Aspergillus terreus）
<400> 47
Met Ser Ser Val Ile Val Ala Leu Ala Val Leu Val Leu Ser Leu Leu
1 5 1015
Tyr Leu Thr Leu Phe Arg Asn Asp Leu Thr His Leu Ile Ile Glu Lys
202530
Leu Gln Ser Phe Arg Thr Gly Ser Gly Trp Glu Leu Ser Pro Arg Ser
354045
Arg Leu Leu Pro Arg Ala Thr Lys Ala Ala Leu Ser Ser Ile Thr Gly
505560
Thr Gly Val Gly Ile Trp Ser Arg Leu Tyr Ala Arg Ile Phe His Ser
65707580
Asp Glu Leu Ala Glu Glu Glu Asp Asp Glu Lys Tyr Gln Ala Gly Glu
859095
Ala Tyr Gly Asp Pro Lys Val Leu Ala Thr Ser Leu Ile Lys Asp Leu
100 105 110
Arg Ala Leu Gly Val Lys Gly Arg Arg Ser Asp Leu Arg Thr Leu Ile
115 120 125
Glu Met Val Lys Asn Lys Gly Lys Pro Met Asp Asp Arg Gln Met His
130 135 140
Met Glu Lys Ile Ile Ala Ile Val Ala Met Leu Pro Arg Thr Ser Lys
145 150 155 160
Ala Arg Gln Arg Leu Thr Gly Val Leu Ile Asp Gln Leu Trp Arg Ser
165 170 175
Leu Gln His Pro Pro Leu Ser Tyr Phe Gly Asn Lys Tyr Gln Tyr Arg
180 185 190
Thr Pro Asp Gly Ser Tyr Asn Asn Pro Leu Glu Pro Asn Leu Gly Lys
195 200 205
Ala Gly Ser Pro Tyr Ala Arg Ser Ile Pro Arg Ile Lys Thr Met His
210 215 220
Gly Val Arg Pro Asp Pro Gly Leu Leu Phe Asp Leu Leu Met Ala Arg
225 230 235 240
Asp Asp Ser Thr Phe Lys Glu Asn Pro Ala Gly Ile Ser Ser Met Leu
245 250 255
Phe Tyr His Ala Ser Ile Ile Ile His Asp Ile Phe Arg Thr Asn Arg
260 265 270
Arg Asp Pro Asn Ile Ser Asp Thr Ser Ser Tyr Leu Asp Leu Ala Pro
275 280 285
Leu Tyr Gly Ser Ser Leu Glu Asp Gln Leu Lys Val Arg Thr Met Glu
290 295 300
Lys Gly Met Leu Lys Pro Asp Thr Phe His Glu Lys Arg Leu Leu Gly
305 310 315 320
Gln Pro Ala Gly Val Asn Val Ile Leu Val Met Tyr Ser Arg Phe His
325 330 335
Asn Tyr Val Ala Asp Met Leu Leu Lys Ile Asn Glu Asn Gly Arg Phe
340 345 350
Thr Leu Pro Pro Thr Ser Ser Glu Glu Ala Arg Lys Lys Ala Leu Ala
355 360 365
Lys Gln Asp Glu Asp Leu Phe Gln Val Ala Arg Leu Val Val Asn Gly
370 375 380
Leu Tyr Val Asn Ile Ser Leu His Asp Tyr Leu Arg Gly Leu Thr Asn
385 390 395 400
Thr His His Ser Ala Ser Asp Trp Thr Leu Asp Pro Arg Ile Ala Val
405 410 415
Gly Arg Thr Phe Asp Pro Asp Gly Val Pro Arg Gly Ile Gly Asn Gln
420 425 430
Ile Ser Ala Glu Phe Asn Leu Leu Tyr Arg Phe His Ser Val Ile Ser
435 440 445
Arg Arg Asp Glu Lys Trp Thr Asn Glu Phe Leu Lys Ser Leu Phe Pro
450 455 460
Asp Leu Asn Lys Pro Leu Asp Gln Leu Thr Pro Gln Glu Phe Met Met
465 470 475 480
Gly Leu Met Arg Tyr Glu Gln Ser Ile Asp Lys Asp Pro Ser Lys Arg
485 490 495
Glu Phe Gly Gly Leu Lys Arg Ser Pro Asp Gly Lys Phe Asn Asp Ala
500 505 510
Asp Leu Val Gln Ile Leu Lys Asp Ser Met Glu Asp Pro Ala Gly Leu
515 520 525
Phe Gly Pro Arg Asn Val Pro Lys Ala Leu Arg Met Ile Glu Ile Ala
530 535 540
Gly Ile Met Ser Ala Arg Lys Trp Asp Leu Gly Ser Leu Asn Glu Met
545 550 555 560
Arg Asp Phe Phe Lys Leu Lys Arg His Ala Thr Phe Glu Asp Ile Asn
565 570 575
Pro Asp Pro Glu Ile Ala Asp Leu Leu Arg Lys Leu Tyr Asp His Pro
580 585 590
Asp Met Val Glu Met Tyr Pro Gly Met Phe Leu Glu Asp Ala Lys Pro
595 600 605
Arg Leu Asp Pro Gly Cys Gly Gly Cys Pro Pro Tyr Thr Val Gly Arg
610 615 620
Ala Val Phe Ser Asp Ala Val Thr Leu Val Arg Ser Asp Arg Phe Leu
625 630 635 640
Thr Leu Asp Tyr Thr Ala Ser Asn Leu Thr Asn Trp Gly Phe Arg Glu
645 650 655
Val Gln Gln Asp Tyr Asp Ile Leu Gly Gly Ser Met Phe His Lys Leu
660 665 670
Ile Gln Arg Ala Leu Pro Gly Trp Phe Pro Tyr Asn Ser Leu His Ala
675 680 685
Thr Gln Pro Met Phe Thr Arg Lys Met Asn Glu Gln Ile Ala Arg Glu
690 695 700
Ile Gly Thr Ile Asp His Tyr Ser Leu Ala Asp Pro Ala Pro Pro Pro
705 710 715 720
Arg Lys Ile Val Leu Thr Asp Tyr Ala Thr Asn Ile Lys Val Leu Lys
725 730 735
Asp Gln Ala Ser Phe Arg Val Pro Trp Ala Arg Tyr Leu Asn Asp Met
740 745 750
Phe Pro Gly Lys Thr Tyr Asn Asp Tyr Met Leu Gly Gly Asp Asp Pro
755 760 765
Ala Asn Ala Ala Gln Lys Lys Leu Val His Ser Ile Leu Phe Ser Pro
770 775 780
Asp Gln Phe Leu Asp Leu Leu Ser Glu Thr Thr Thr Lys Leu Gly Ser
785 790 795 800
Glu Leu Leu Lys Ala Asn Thr Leu Trp Leu Thr Lys Asp Leu His Gln
805 810 815
Val Asp Ile Ile Arg Asp Val Ala Ile Pro Leu Asn Ala Arg Ile Met
820 825 830
Ala Asp Leu Phe Cys Leu Asp Met Lys Thr Pro Glu Asn Pro Thr Gly
835 840 845
Ser Met Asn Ala Ala Thr Val Tyr Arg His Leu Met Asn Val Arg Ile
850 855 860
Trp Gly Phe Asn Asn Asn Asp Pro Ala Leu Met Leu Gln Arg Arg Lys
865 870 875 880
Trp Ala Ile Glu Ser Ala Glu Ala Leu Ile Glu Thr Thr Arg Lys Leu
885 890 895
Val Asn Glu Gln Ala Gln Pro Ala Gln Ser Gly Val Leu Lys Asn Leu
900 905 910
Met Thr Arg Arg Gln Ala Thr Gly Thr Leu Arg Trp Tyr Gly Asn Asn
915 920 925
Val Ala Lys Glu Met Met Glu Met Gly Met Ser Ala Glu Glu Val Ala
930 935 940
Asp Ile Cys Trp Leu Thr Ala Ile Gly Gly Val Gly Thr Pro Ser Gly
945 950 955 960
Val Val Ala Asn Val Leu Gln Tyr Tyr Phe Arg Tyr Glu Asn Ile Gly
965 970 975
His Trp Glu Glu Ile Gln Lys Leu Val Thr Gln Pro Asp Thr Pro Ala
980 985 990
Ala Asp Arg Thr Leu Arg Gln Tyr Val Leu Glu Ala Asn Arg Leu Thr
995 10001005
Ser Met Glu Cys Thr Val Arg Val Cys Ala Arg Pro Val Thr Val
101010151020
Asp Gly His Asp Phe Lys Pro Gly Glu Val Ile Val Asn His Leu
102510301035
Gly Leu Ala Cys Arg Asp Pro His Asn Ile Pro Asp Ala Asp Lys
104010451050
Phe Arg Leu Asp Arg Pro Ala Ser Ala Tyr Ile Gln Trp Gly Tyr
105510601065
Gly Ala His Glu Cys Leu Gly Lys Glu Ile Ala Ile Thr Phe Ala
107010751080
Val Ser Met Ile Arg Ile Leu Ala Gly Leu Lys Tyr Leu Arg Pro
108510901095
Ala Pro Gly Glu Met Gly Val Leu Lys Ser Val Met Ala Asp Gly
110011051110
Arg Gln Ala Phe Leu Asn Asp Ser Trp Ser Trp Leu Thr Gln Asp
111511201125
Pro Thr Ser Lys Ser Asn Met His Gly Lys Ala Ser Ala Val Asp
113011351140
<210> 48
<211> 518
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 48
Met Ala Ser Ile Ser Thr Pro Phe Pro Ile Ser Leu His Pro Lys Thr
1 5 1015
Val Arg Ser Lys Pro Leu Lys Phe Arg Val Leu Thr Arg Pro Ile Lys
202530
Ala Ser Gly Ser Glu Thr Pro Asp Leu Thr Val Ala Thr Arg Thr Gly
354045
Ser Lys Asp Leu Pro Ile Arg Asn Ile Pro Gly Asn Tyr Gly Leu Pro
505560
Ile Val Gly Pro Ile Lys Asp Arg Trp Asp Tyr Phe Tyr Asp Gln Gly
65707580
Ala Glu Glu Phe Phe Lys Ser Arg Ile Arg Lys Tyr Asn Ser Thr Val
859095
Tyr Arg Val Asn Met Pro Pro Gly Ala Phe Ile Ala Glu Asn Pro Gln
100 105 110
Val Val Ala Leu Leu Asp Gly Lys Ser Phe Pro Val Leu Phe Asp Val
115 120 125
Asp Lys Val Glu Lys Lys Asp Leu Phe Thr Gly Thr Tyr Met Pro Ser
130 135 140
Thr Glu Leu Thr Gly Gly Tyr Arg Ile Leu Ser Tyr Leu Asp Pro Ser
145 150 155 160
Glu Pro Lys His Glu Lys Leu Lys Asn Leu Leu Phe Phe Leu Leu Lys
165 170 175
Ser Ser Arg Asn Arg Ile Phe Pro Glu Phe Gln Ala Thr Tyr Ser Glu
180 185 190
Leu Phe Asp Ser Leu Glu Lys Glu Leu Ser Leu Lys Gly Lys Ala Asp
195 200 205
Phe Gly Gly Ser Ser Asp Gly Thr Ala Phe Asn Phe Leu Ala Arg Ala
210 215 220
Phe Tyr Gly Thr Asn Pro Ala Asp Thr Lys Leu Lys Ala Asp Ala Pro
225 230 235 240
Gly Leu Ile Thr Lys Trp Val Leu Phe Asn Leu His Pro Leu Leu Ser
245 250 255
Ile Gly Leu Pro Arg Val Ile Glu Glu Pro Leu Ile His Thr Phe Ser
260 265 270
Leu Pro Pro Ala Leu Val Lys Ser Asp Tyr Gln Arg Leu Tyr Glu Phe
275 280 285
Phe Leu Glu Ser Ala Gly Glu Ile Leu Val Glu Ala Asp Lys Leu Gly
290 295 300
Ile Ser Arg Glu Glu Ala Thr His Asn Leu Leu Phe Ala Thr Cys Phe
305 310 315 320
Asn Thr Trp Gly Gly Met Lys Ile Leu Phe Pro Asn Met Val Lys Arg
325 330 335
Ile Gly Arg Ala Gly His Gln Val His Asn Arg Leu Ala Glu Glu Ile
340 345 350
Arg Ser Val Ile Lys Ser Asn Gly Gly Glu Leu Thr Met Gly Ala Ile
355 360 365
Glu Lys Met Glu Leu Thr Lys Ser Val Val Tyr Glu Cys Leu Arg Phe
370 375 380
Glu Pro Pro Val Thr Ala Gln Tyr Gly Arg Ala Lys Lys Asp Leu Val
385 390 395 400
Ile Glu Ser His Asp Ala Ala Phe Lys Val Lys Ala Gly Glu Met Leu
405 410 415
Tyr Gly Tyr Gln Pro Leu Ala Thr Arg Asp Pro Lys Ile Phe Asp Arg
420 425 430
Ala Asp Glu Phe Val Pro Glu Arg Phe Val Gly Glu Glu Gly Glu Lys
435 440 445
Leu Leu Arg His Val Leu Trp Ser Asn Gly Pro Glu Thr Glu Thr Pro
450 455 460
Thr Val Gly Asn Lys Gln Cys Ala Gly Lys Asp Phe Val Val Leu Val
465 470 475 480
Ala Arg Leu Phe Val Ile Glu Ile Phe Arg Arg Tyr Asp Ser Phe Asp
485 490 495
Ile Glu Val Gly Thr Ser Pro Leu Gly Ser Ser Val Asn Phe Ser Ser
500 505 510
Leu Arg Lys Ala Ser Phe
515
<210> 49
<211> 1066
<212> PRT
<213> 海鞭（Plexaura homomalla）
<400> 49
Met Thr Trp Lys Asn Phe Gly Phe Glu Ile Phe Gly Glu Lys Tyr Gly
1 5 1015
Gln Glu Glu Leu Glu Lys Arg Ile Lys Asp Glu His Thr Pro Pro Pro
202530
Asp Ser Pro Val Phe Gly Gly Leu Lys Leu Lys Leu Lys Lys Glu Lys
354045
Phe Lys Thr Leu Phe Thr Leu Gly Thr Thr Leu Lys Gly Phe Arg Arg
505560
Ala Thr His Thr Val Gly Thr Gly Gly Ile Gly Glu Ile Thr Ile Val
65707580
Asn Asp Pro Lys Phe Pro Glu His Glu Phe Phe Thr Ala Gly Arg Thr
859095
Phe Pro Ala Arg Leu Arg His Ala Asn Leu Lys Tyr Pro Asp Asp Ala
100 105 110
Gly Ala Asp Ala Arg Ser Phe Ser Ile Lys Phe Ala Asp Ser Asp Ser
115 120 125
Asp Gly Pro Leu Asp Ile Val Met Asn Thr Gly Glu Ala Asn Ile Phe
130 135 140
Trp Asn Ser Pro Ser Leu Glu Asp Phe Val Pro Val Glu Glu Gly Asp
145 150 155 160
Ala Ala Glu Glu Tyr Val Tyr Lys Asn Pro Tyr Tyr Tyr Tyr Asn Leu
165 170 175
Val Glu Ala Leu Arg Arg Ala Pro Asp Thr Phe Ala His Leu Tyr Tyr
180 185 190
Tyr Ser Gln Val Thr Met Pro Phe Lys Ala Lys Asp Gly Lys Val Arg
195 200 205
Tyr Cys Arg Tyr Arg Ala Leu Pro Gly Asp Val Asp Ile Lys Glu Glu
210 215 220
Asp Glu Ser Gly Arg Leu Thr Glu Glu Glu Gln Arg Lys Ile Trp Ile
225 230 235 240
Phe Ser Arg His Glu Asn Glu Lys Arg Pro Asp Asp Tyr Leu Arg Lys
245 250 255
Glu Tyr Val Glu Arg Leu Gln Lys Gly Pro Val Asn Tyr Arg Leu Gln
260 265 270
Ile Gln Ile His Glu Ala Ser Pro Asp Asp Thr Ala Thr Ile Phe His
275 280 285
Ala Gly Ile Leu Trp Asp Lys Glu Thr His Pro Trp Phe Asp Leu Ala
290 295 300
Lys Val Ser Ile Lys Thr Pro Leu Ser Pro Asp Val Leu Glu Lys Thr
305 310 315 320
Ala Phe Asn Ile Ala Asn Gln Pro Ala Ser Leu Gly Leu Leu Glu Ala
325 330 335
Lys Ser Pro Glu Asp Tyr Asn Ser Ile Gly Glu Leu Arg Val Ala Val
340 345 350
Tyr Thr Trp Val Gln His Leu Arg Lys Leu Lys Ile Gly Ser Leu Val
355 360 365
Pro Ala Gly Gln Asn Ala Ile Tyr Asn Val Glu Val Glu Thr Gly Asp
370 375 380
Arg Glu His Ala Gly Thr Asp Ala Thr Ile Thr Ile Arg Ile Thr Gly
385 390 395 400
Ala Lys Gly Arg Thr Asp Tyr Leu Lys Leu Asp Lys Trp Phe His Asn
405 410 415
Asp Phe Glu Ala Gly Ser Lys Glu Gln Tyr Thr Val Gln Gly Phe Asp
420 425 430
Val Gly Asp Ile Gln Leu Ile Glu Leu His Ser Asp Gly Gly Gly Tyr
435 440 445
Trp Ser Gly Asp Pro Asp Trp Phe Val Asn Arg Val Ile Ile Ile Ser
450 455 460
Ser Thr Gln Asp Arg Val Tyr Ser Phe Pro Cys Phe Arg Trp Val Ile
465 470 475 480
Lys Asp Met Val Leu Phe Pro Gly Glu Ala Thr Leu Pro Phe Asn Glu
485 490 495
Val Pro Ala Ile Val Ser Glu Gln Arg Gln Lys Glu Leu Glu Gln Arg
500 505 510
Lys Leu Thr Tyr Gln Trp Asp Tyr Val Ser Asp Asp Met Pro Gly Asn
515 520 525
Ile Lys Ala Lys Thr His Asp Asp Leu Pro Arg Asp Val Gln Phe Thr
530 535 540
Asp Glu Lys Ser Arg Ser Tyr Gln Glu Ser Arg Lys Ala Ala Leu Val
545 550 555 560
Asn Leu Gly Ile Gly Ser Leu Phe Thr Met Phe Glu Asn Trp Asp Ser
565 570 575
Tyr Asp Asp Tyr His Ile Leu Tyr Arg Asn Trp Ile Leu Gly Gly Thr
580 585 590
Pro Asn Met Ala Asp Arg Trp His Glu Asp Arg Trp Phe Gly Tyr Gln
595 600 605
Phe Leu Asn Gly Ala Asn Pro Val Ile Leu Thr Arg Cys Asp Ala Leu
610 615 620
Pro Ser Asn Phe Pro Val Thr Asn Glu His Val Asn Ala Ser Leu Asp
625 630 635 640
Arg Gly Lys Asn Leu Asp Glu Glu Ile Lys Asp Gly His Ile Tyr Ile
645 650 655
Val Asp Phe Lys Val Leu Val Gly Ala Lys Ser Tyr Gly Gly Pro Val
660 665 670
Leu Glu Asp Ile Gly Tyr Lys Val Pro Asp His Leu Lys His Asp Glu
675 680 685
Ala Asp Ile Arg Tyr Cys Ala Ala Pro Leu Ala Leu Phe Tyr Val Asn
690 695 700
Lys Leu Gly His Leu Met Pro Ile Ala Ile Gln Ile Asn Gln Glu Pro
705 710 715 720
Gly Pro Glu Asn Pro Ile Trp Thr Pro His Glu Glu Asn Glu His Asp
725 730 735
Trp Met Met Ala Lys Phe Trp Leu Gly Val Ala Glu Ser Asn Phe His
740 745 750
Gln Leu Asn Thr His Leu Leu Arg Thr His Leu Thr Thr Glu Ser Phe
755 760 765
Ala Leu Ser Thr Trp Arg Asn Leu Ala Ser Ala His Pro Val Phe Lys
770 775 780
Leu Leu Gln Pro His Ile Tyr Gly Val Leu Ala Ile Asp Thr Ile Gly
785 790 795 800
Arg Lys Glu Leu Ile Gly Ser Gly Gly Ile Val Asp Gln Ser Leu Ser
805 810 815
Leu Gly Gly Gly Gly His Val Thr Phe Met Glu Lys Cys Phe Lys Glu
820 825 830
Val Asn Leu Gln Asp Tyr His Leu Pro Asn Ala Leu Lys Lys Arg Gly
835 840 845
Val Asp Asp Pro Ser Lys Leu Pro Gly Phe Tyr Tyr Arg Asp Asp Gly
850 855 860
Leu Ala Leu Trp Glu Ala Ile Glu Thr Phe Ile Gly Glu Ile Ile Ala
865 870 875 880
Ile Phe Tyr Lys Asn Asp Asp Asp Val Lys Arg Asp Asn Glu Ile Gln
885 890 895
Ser Trp Ile Tyr Asp Val His Lys Asn Gly Trp Arg Val Asn Pro Gly
900 905 910
His Gln Asp His Gly Val Pro Ala Ser Phe Glu Ser Arg Glu Gln Leu
915 920 925
Lys Glu Val Leu Thr Ser Leu Val Phe Thr Phe Ser Cys Gln His Ala
930 935 940
Ala Val Asn Phe Ser Gln Lys Asp His Tyr Gly Phe Thr Pro Asn Ala
945 950 955 960
Pro Ala Val Leu Arg His Pro Pro Pro Lys Lys Lys Gly Glu Ala Thr
965 970 975
Leu Gln Ser Ile Leu Ser Thr Leu Pro Ser Lys Ser Gln Ala Ala Lys
980 985 990
Ala Ile Ala Thr Val Tyr Ile Leu Thr Lys Phe Ser Glu Asp Glu Arg
995 10001005
Tyr Leu Gly Asn Tyr Ser Ala Thr Ala Trp Glu Asp Lys Asp Ala
101010151020
Leu Asp Ala Ile Asn Arg Phe Gln Asp Lys Leu Glu Asp Ile Ser
102510301035
Lys Lys Ile Lys Gln Arg Asn Glu Asn Leu Glu Val Pro Tyr Ile
104010451050
Tyr Leu Leu Pro Glu Arg Ile Pro Asn Gly Thr Ala Ile
105510601065
<210> 50
<211> 1151
<212> PRT
<213> 尖孢镰孢菌（Fusarium oxysporum）
<400> 50
Met Ser Phe Asn Glu Lys Phe Gln Ala Gly Glu Ser Tyr Gly Asp Ser
1 5 1015
Lys Glu Asp Pro Ser Ser Leu Leu Asn Asn Pro Glu Lys Leu Val Ala
202530
Asp Leu Met Lys Asp Phe Ala Gly Val Arg Ser Gln Ala Ser Pro Ala
354045
Gln Leu Leu Gly Leu Val Lys Glu Leu Leu Gln Lys Gly Gln Pro Leu
505560
Asp Asp Lys Lys Gly Thr Thr Glu Leu Leu Ile Gly Ile Leu Thr Ala
65707580
Leu Pro Ala Thr Ser Lys Ala Arg Thr Ala Leu Thr Asn Lys Leu Ile
859095
Asp Thr Leu Trp Gly Asn Leu Gln His Pro Pro Leu Ser Tyr Met Gly
100 105 110
Gly Asp Val Lys Tyr Asp Val Val Asn Ser Asp Lys Pro Ala His Lys
115 120 125
His Asn Cys Glu Leu Tyr Asp Thr Ile Glu Phe Lys Val Pro Gly Thr
130 135 140
Asp Val Leu Leu Arg Glu Gln Val Pro Gln Ala Pro Asp Gly Leu His
145 150 155 160
Gln Tyr Arg Met Pro Asp Gly Ser Phe Asn Asn Ile Leu Glu Pro Asn
165 170 175
Leu Gly Arg Ala Gly Thr Pro Tyr Ala Lys Ser Val Lys Ser Glu Lys
180 185 190
Arg Leu His Gly Val Lys Pro Asp Pro Gly Leu Leu Phe Asp Leu Leu
195 200 205
Met Ala Arg Asp Glu Thr Thr Phe Gln Glu Asn Pro Ala Gly Ile Ser
210 215 220
Ser Met Leu Phe Tyr His Ala Ala Ile Ile Ile His Asp Ile Phe Arg
225 230 235 240
Thr Asn Arg Thr Asp Met Asn Lys Ser Asp Thr Ser Ser Tyr Leu Asp
245 250 255
Leu Ala Pro Leu Tyr Gly Ser Ser Leu Lys Asp Gln His Glu Ile Arg
260 265 270
Thr Met Lys Glu Gly Lys Leu Lys Pro Asp Thr Phe His Glu Lys Arg
275 280 285
Leu Leu Gly Gln Pro Ala Gly Val Asn Val Met Leu Val Leu Tyr Ser
290 295 300
Arg Phe His Asn Tyr Val Ala Asp Ile Leu Leu Lys Ile Asn Glu Asn
305 310 315 320
Gly Arg Phe Ser Leu Ser Val Pro Pro Asn Ala Ser Glu Glu Asp Lys
325 330 335
Ala Lys Ala Ile Ala Lys Gln Asp His Asp Leu Phe Asn Val Ala Arg
340 345 350
Leu Ile Thr Gly Gly Leu Tyr Ile Asn Ile Cys Leu His Asp Tyr Leu
355 360 365
Arg Ala Ile Thr Asn Thr His His Ser Ala Ser Asp Trp Thr Leu Asp
370 375 380
Pro Arg Val Ala Ile Asp Lys Gln Phe Asp Gly Asp Gly Val Pro Arg
385 390 395 400
Gly Val Gly Asn Gln Val Ser Val Glu Phe Asn Leu Leu Tyr Arg Phe
405 410 415
His Ser Cys Ile Ser Lys Arg Asp Glu Lys Trp Ile Asn Asn Phe Phe
420 425 430
Leu Lys Leu Phe Pro Gly Arg Lys Ala Glu Asp Leu Gln Asp Val Ser
435 440 445
Trp Thr Glu Leu Gly Gln Ala Leu Leu Ile Phe Glu Gln Asn Thr Pro
450 455 460
Lys Asp Pro Ser Val Arg Thr Phe Asp Gly Leu Glu Arg Gln Ala Asp
465 470 475 480
Gly Thr Phe Lys Asp Glu Asp Leu Val Arg Ile Leu Lys Asp Ala Met
485 490 495
Glu Asp Pro Ala Gly Thr Phe Gly Ala Arg Met Val Pro Lys Ala Leu
500 505 510
Lys Val Val Glu Val Leu Gly Ile Ile Gln Gly Arg Lys Trp Gln Cys
515 520 525
Ala Ser Leu Asn Glu Phe Arg Glu Phe Phe Gly Leu Lys Arg Tyr Asp
530 535 540
Ser Phe Ser Glu Ile Asn Ser Asn Pro Asp Ile Ala Asn Ile Leu Glu
545 550 555 560
Lys Leu Tyr Thr Asp Pro Asp Met Val Glu Leu Tyr Pro Gly Leu Met
565 570 575
Ile Glu Asp Ile Lys Pro Gln Arg Asn Pro Gly Ser Gly Ile Met Pro
580 585 590
Thr Tyr Ser Val Gly Arg Ala Val Leu Ser Asp Ala Val Thr Leu Val
595 600 605
Arg Ser Asp Arg Phe Asn Thr Ile Asp Tyr Thr Val Ser Asn Leu Thr
610 615 620
Ala Trp Gly Tyr Asn Glu Val Gln Gln Asp Tyr Lys Thr Leu Gly Gly
625 630 635 640
Ser Met Leu Tyr Lys Leu Ile Gln Arg Gly Val Pro Asn Trp Phe Pro
645 650 655
Phe Asn Ser Ile Ala Val Met Gln Pro Met Tyr Thr Lys Lys Ala Asn
660 665 670
Glu Gln Ile Ala Lys Glu Ile Gly Thr Phe Asp Gln Tyr Thr Leu Asp
675 680 685
Asp Pro Lys Ala Pro Pro Lys Val Ala Val Leu Thr Ser Gly Pro Ala
690 695 700
Ile Lys Gln Ile Leu Ser Asn Thr Lys Gln Tyr Val Val Pro Trp Leu
705 710 715 720
Lys Pro Leu Asn Thr Leu Phe Pro Gly Lys Lys Asp Phe Gly Trp Phe
725 730 735
Met Leu Ala Gly Asp Gln Pro Gln Asn Tyr Thr His Arg Ala Asn Phe
740 745 750
Ser Lys Ala Met Ser Lys Ile Pro Asn Met His Asn Ala Val His Ala
755 760 765
Phe Ile Glu Arg Glu Gly Thr Lys Leu Ile Asn Lys Glu Thr Phe Thr
770 775 780
Leu Lys Lys Gly Leu Asp Gln Ile Asp Ile Ile Arg Asp Val Ala Ile
785 790 795 800
Pro Leu Asn Thr Gln Leu Leu Ala Asp Leu Phe Tyr Phe Asp Leu Arg
805 810 815
Thr Glu Glu Asn Pro Asp Gly Lys Leu Gly Val Ala Glu Leu Tyr Arg
820 825 830
Ser Leu Leu Asp Ile Arg Ile Trp Gly Val Asn Asn Asn Asp Pro Ala
835 840 845
Gln Ala Trp Asn Arg Arg Arg Arg Ala Gln Glu Gly Ala Lys Arg Met
850 855 860
Ile Glu Thr Thr Lys Thr Ile Val Ala Glu Ala Asp Ala Gly Arg Pro
865 870 875 880
Arg Gly Ile Gly Leu Val Ser Ala Val Ala Asn Arg Ile Gly Ala Arg
885 890 895
Ser Tyr Leu Lys Lys Asp Ser Leu Arg Ser Cys Gly Leu Lys Leu Val
900 905 910
Glu Glu Leu Leu Ala Gln Gly Asn Asn Val Asp Gln Val Thr Asp Asn
915 920 925
Leu Trp Leu Thr Ala Phe Gly Gly Ile Gly Val Pro Val Thr Ala Phe
930 935 940
Tyr Glu Val Leu Ser Phe Phe Leu Arg Pro Glu Asn Glu Ala Ile Trp
945 950 955 960
Ala Glu Val Gln Ala Ile Ala Gln Lys Gly Asp Asp Ala Thr Leu His
965 970 975
Ala Tyr Val Ala Glu Ala Gln Arg Met Thr Ser Ser Gln Arg Asn Val
980 985 990
Arg Val Ala Thr Ala Pro Gly Glu Val Gln Gly Gln Ala Ile Gln Pro
995 10001005
Gly Thr Ala Val Val Leu Met Leu Gly Glu Ala Gly Arg Asn Pro
101010151020
Lys Glu Val Pro Asp Ala Gly Lys Phe Asn Pro Gln Arg Lys Lys
102510301035
Glu Asp Val Ser Ala Phe Ser Tyr Gly Gln His Glu Cys Ile Ala
104010451050
Lys Asp Val Ala Leu Ala Phe Val Thr Gly Leu Ile Lys Leu Val
105510601065
Ala Asp Leu Lys Glu Leu Arg Pro Ala Pro Gly Gln Met Gly Thr
107010751080
Val Lys Thr Ile Gln Val Gly Thr Glu Lys Ala Tyr Leu Asn Asp
108510901095
Ser Trp Ser Tyr Leu Gly Phe Asp Ala Ser Thr Trp Lys Val His
110011051110
Phe Asn Gly His Gly Lys Gly Lys Phe Glu Gly Glu Arg Val Pro
111511201125
Thr Lys Ser Thr Pro Ile Gln Glu Tyr Tyr Tyr Leu Leu Gln Lys
113011351140
Arg Lys Asp Glu Ile Leu Gly Asn
11451150
<210> 51
<211> 1162
<212> PRT
<213> 禾本科炭疽刺盘孢（Colletotrichum graminicola）
<400> 51
Met Ser Phe Asp Gln Lys Phe Met Ala Gly Glu Thr Tyr Gly Asp Glu
1 5 1015
Arg Lys Ser Ser Ala Asn Phe Phe Ser Asp Pro Thr Lys Val Ala Thr
202530
Asp Leu Leu Lys Asp Tyr Ala Gly Leu Arg Ser Gln Thr Ser Pro Ala
354045
Glu Leu Ala Gly Leu Ile Lys Glu Leu Leu Gln Lys Gly Gln Pro Leu
505560
Asp Asp Lys Lys Gly Thr Thr Glu Ile Leu Ile Gly Ile Leu Thr Ser
65707580
Leu Pro Ser Thr Ser Ser Thr Arg Val Gln Leu Thr Asn Lys Leu Ile
859095
Asp Thr Leu Trp Gly Thr Leu Gln His Pro Pro Leu Ser Tyr Val Gly
100 105 110
Gly Asp Val Lys Tyr Glu Val Val Asn Pro Asn Glu Thr Ala Ser Lys
115 120 125
Asp Lys Gln Gln Thr Glu Asp Ser Ile Glu Phe Lys Ala Pro Asp Ser
130 135 140
Asp Val Ile Leu Arg Glu Gln Val Pro Arg Pro Pro Asp Gly Leu His
145 150 155 160
His Tyr Arg Met Pro Asp Gly Ser Tyr Asn Asn Ile Leu Glu Pro Asn
165 170 175
Leu Gly Lys Ala Gly Thr Pro Tyr Ala Lys Thr Val Arg Thr Ser Lys
180 185 190
Arg Leu His Gly Val Lys Pro Asp Pro Gly Leu Leu Phe Asp Leu Leu
195 200 205
Met Ala Arg Asp Asp Ser Thr Phe Lys Glu Asn Pro Ala Gly Ile Ser
210 215 220
Ser Met Leu Phe Tyr His Ala Ser Ile Ile Ile His Asp Ile Phe Arg
225 230 235 240
Thr Asn Arg Thr Asp Met Asn Lys Ser Asp Thr Ser Ser Tyr Leu Asp
245 250 255
Leu Ala Pro Leu Tyr Gly Ser Ser Leu Lys Asp Gln Leu Glu Ile Arg
260 265 270
Thr Met Lys Glu Gly Met Leu Lys Pro Asp Thr Phe His Glu Arg Arg
275 280 285
Leu Leu Gly Gln Pro Ala Gly Val Asn Ala Met Leu Val Leu Tyr Asn
290 295 300
Arg Phe His Asn Tyr Val Cys Asp Ile Leu Leu Lys Ile Asn Glu Asn
305 310 315 320
Gly Arg Phe Thr Leu Gln Cys Pro Ala Asp Ala Ser Pro Glu Asp Arg
325 330 335
Ala Lys Ala Val Ala Lys Gln Asp His Asp Leu Phe Asn Thr Ala Arg
340 345 350
Leu Ile Val Gly Gly Leu Tyr Ile Asn Ile Ser Leu His Asp Tyr Leu
355 360 365
Arg Ala Ile Thr Asn Thr His His Ser Lys Ser Asp Trp Thr Leu Asp
370 375 380
Pro Arg Val Glu Ile Gly Lys Gln Phe Asp Gly Glu Gly Val Pro Arg
385 390 395 400
Gly Val Gly Asn Gln Val Ser Val Glu Phe Asn Leu Leu Tyr Arg Phe
405 410 415
His Ser Cys Ile Ser Lys Lys Asp Glu Arg Trp Ile Asp Asn Phe Phe
420 425 430
Ala Lys Leu Phe Pro Asp Arg Lys Pro Glu Asp Leu Gln Asn Val Gly
435 440 445
Met Ala Glu Leu Gly Gln Ala Leu Met Thr Phe Glu Gln Ser Ile Pro
450 455 460
Lys Asp Pro Ser Ala Arg Thr Phe Asp Asn Leu Glu Arg Gln Ala Asp
465 470 475 480
Gly Lys Phe Lys Asp Glu Asp Leu Val Arg Val Leu Lys Glu Ala Met
485 490 495
Asp Asp Pro Ala Gly Cys Phe Gly Ala Arg Met Val Pro Lys Ala Leu
500 505 510
Lys Ile Val Glu Ile Leu Gly Ile Asn Gln Ala Arg Lys Trp Gln Val
515 520 525
Ala Ser Leu Asn Glu Phe Arg Glu Phe Phe Gly Leu Lys Lys Tyr Asp
530 535 540
Lys Phe Ala Glu Ile Asn Ser Asn Pro Glu Ile Ala Thr Leu Leu Glu
545 550 555 560
Lys Leu Tyr Thr Asp Pro Asp Met Val Glu Leu Tyr Pro Gly Leu Met
565 570 575
Ile Glu Asp Ile Lys Pro Ala Arg Asn Thr Gly Ser Gly Ile Cys Pro
580 585 590
Thr Tyr Ser Val Gly Arg Ala Val Leu Ser Asp Ala Val Thr Leu Val
595 600 605
Arg Ser Asp Arg Phe Asn Thr Leu Asp Tyr Thr Val Ser Asn Leu Thr
610 615 620
Ala Trp Gly Tyr Asn Glu Val Gln Gln Asp Tyr Lys Thr Leu Gly Gly
625 630 635 640
Ser Met Leu Tyr Lys Leu Ile Gln Arg Gly Leu Pro Asn Trp Phe Pro
645 650 655
Tyr Asn Ser Val Ala Val Met Gln Pro Met Tyr Thr Lys Lys Ala Asn
660 665 670
Glu Ser Ile Ala Lys Glu Leu Gly Thr Leu His Leu Tyr Thr Ser Asn
675 680 685
Asp Pro Lys Pro Pro Pro Lys Ile Val Val Val Ala Thr Ser Val Ala
690 695 700
Ile Lys Gln Val Leu Gly Asn Ser Lys Gln Phe Val Val Pro Trp Leu
705 710 715 720
Gln Pro Leu Asn Asp Leu Phe Thr Gly Thr Lys Lys Asp Ile Ser Trp
725 730 735
Phe Met Leu Ala Gly Asp Glu Pro Lys Asn Tyr Gln His Arg Val Asn
740 745 750
Leu Leu Lys Ala Met Gly Lys Ile Pro Asn Leu His Asn Ala Val His
755 760 765
Glu Phe Val Asp Arg Val Gly Ala Lys Leu Ile Glu Lys Glu Thr Phe
770 775 780
Lys Leu Lys Glu Gly Leu Cys Gln Met Asp Ile Ile Arg Asp Val Ala
785 790 795 800
Ile Pro Leu Asn Ala Gln Leu Leu Ala Asp Leu Phe Tyr Phe Asp Met
805 810 815
Arg Thr Asp Glu Asn Pro Asn Gly Thr Leu Ser Ala Ala Asp Leu Tyr
820 825 830
Arg His Leu Leu Asn Ile Arg Val Trp Gly Val Asn Asn Asn Asp Pro
835 840 845
Ala Gln Ala Trp Asn Arg Arg Arg Arg Ala Thr Glu Ser Val Asn Ala
850 855 860
Ile Ile Asp Ser Thr Arg Gly Leu Val Asn Glu Val Val Val Gly Arg
865 870 875 880
Gly Leu Gly Phe Gly Ile Ala Ser Thr Ile Ser Gly Val Val Gly Arg
885 890 895
Lys Ser Asn Phe Lys Lys Asp Ser Leu Arg Ser Cys Gly His Lys Leu
900 905 910
Val Glu Glu Leu Leu Ala Gln Gly Asn Ser Ala Glu Gln Val Val Asp
915 920 925
Asn Met Trp Leu Thr Ala Phe Gly Gly Ile Gly Ala Pro Val Thr Ala
930 935 940
Phe Tyr Glu Val Leu Glu Tyr Phe Leu Arg Arg Glu Asn Ala Ser Ile
945 950 955 960
Trp Ala Glu Val Gln Thr Leu Ala Gln Lys Asn Asp Asp Ala Gly Leu
965 970 975
His Ala Tyr Val Lys Glu Ala Gln Arg Leu Thr Ser Ser Gln Arg Asn
980 985 990
Val Arg Val Ala Thr Val Ala Ala Glu Val Asp Gly Lys Gln Val Gln
995 10001005
Pro Gly Asn Val Val Val Met Leu Leu Gly Asp Ala Gly Arg Asn
101010151020
Pro Lys Glu Val Ala Asn Pro Asp Lys Phe Asp Ala Lys Arg Lys
102510301035
Thr Asp Pro Val Ser Ala Phe Ser Tyr Gly Gln His Glu Cys Leu
104010451050
Ala Lys Asp Ile Ala Thr Thr Phe Ile Val Gly Leu Val Lys Leu
105510601065
Val Ala Asp Leu Lys Gln Leu Arg Pro Ala Pro Gly Gln Met Gly
107010751080
Leu Val Lys Thr Ile Arg Val Gly Ser Glu Lys Ala Tyr Leu Asn
108510901095
Asp Ser Trp Ser Tyr Leu Gly Phe Asp Ala Ser Thr Trp Lys Val
110011051110
His Phe Asp Gly His Gly Lys Gly Asn Phe Glu Gly Asp Val Gln
111511201125
Pro Asn Lys Pro Ile Asp Leu Gly Gln Tyr Tyr Tyr Leu Leu Gln
113011351140
Lys Arg Lys Glu Lys Leu Leu Asn Gly Asn Ser Ser Asn Gly Thr
114511501155
Asn Gly Ser Ser
1160
<210> 52
<211> 1161
<212> PRT
<213> 苹果炭疽病菌（Glomerella cingulate）
<400> 52
Met Ala Phe Asn Thr Lys Phe Gln Ala Gly Glu Ser Tyr Gly Asp Glu
1 5 1015
Arg Lys Gly Ser Asn Asn Phe Phe Ala Asp Pro Lys Lys Val Ala Met
202530
Asp Ile Ile Lys Asp Leu Gly Gly Ala His Gly Gln Leu Asn Leu Leu
354045
Glu Val Thr Ala Leu Val Gln Gln Leu Leu Gln Lys Gly Glu Pro Leu
505560
Asp Asp Lys Lys Gly Thr Thr Glu Ala Leu Ile Gly Ile Leu Thr Ser
65707580
Leu Pro Ser Gly Ser Asn Thr Arg Val Gln Leu Thr Asn Lys Leu Ile
859095
Asp Thr Leu Trp Gly Asn Leu Gln His Pro Pro Leu Ser Tyr Val Gly
100 105 110
Gly Asp Val Lys Tyr Glu Val Val Lys Thr Lys Glu Gln Leu Ala Lys
115 120 125
Glu Gln Ala Asp Leu Ala Ala Gln Gly Lys Ala Pro Gln Ser Pro Glu
130 135 140
Glu Ser His Ile Thr Phe Lys Ala Pro Asp Phe Pro Asp Ile Thr Leu
145 150 155 160
Arg Glu His Leu Pro Thr Pro Pro Asp Asn Tyr Lys Met Pro Asp Gly
165 170 175
Ser Tyr Asn Asn Ile Leu Glu Pro Asn Leu Gly Ala Ala Gly Thr Pro
180 185 190
Tyr Ala Lys Thr Val Lys Thr Glu Lys Arg Leu Ala Gly Val Lys Pro
195 200 205
Asp Pro Gly Leu Leu Phe Asp Leu Leu Leu Ala Arg Asp Asp Lys Lys
210 215 220
Phe Thr Glu Asn Pro Ala Gly Ile Ser Ser Met Leu Phe Tyr His Ala
225 230 235 240
Ser Ile Ile Ile His Asp Ile Phe Arg Thr Asn Arg His Asp Leu Asn
245 250 255
Lys Ser Asp Thr Ser Ser Tyr Leu Asp Leu Ala Pro Leu Tyr Gly Ser
260 265 270
Ser Phe Lys Asp Gln Leu Glu Ile Arg Thr Met Lys Glu Gly Lys Leu
275 280 285
Lys Pro Asp Thr Phe His Glu Lys Arg Leu Leu Gly Gln Pro Ala Gly
290 295 300
Val Asn Val Met Leu Val Leu Ser Ser Arg Phe His Asn Tyr Val Ala
305 310 315 320
Asp Ile Leu Leu Lys Ile Asn Glu Asn Gly Arg Phe Thr Leu Gln Thr
325 330 335
Ala Lys Asp Ala Ala Pro Glu Asp Gln Ala Lys Ala Val Ala Lys Gln
340 345 350
Asp His Asp Leu Phe Asn Val Ala Arg Leu Val Thr Gly Gly Leu Tyr
355 360 365
Ile Asn Ile Cys Leu His Asp Tyr Leu Arg Ala Ile Thr Asn Thr His
370 375 380
His Ser Lys Ser Asp Trp Thr Leu Asp Pro Arg Val Glu Ile Gly Lys
385 390 395 400
Gln Phe Asp Gly Glu Gly Val Pro Arg Gly Val Gly Asn Gln Val Ser
405 410 415
Val Glu Phe Asn Leu Leu Tyr Arg Phe His Ser Cys Ile Ser Lys Lys
420 425 430
Asp Glu Arg Trp Ile Asp Gly Phe Phe Ala Lys Leu Phe Pro Gly Arg
435 440 445
Lys Pro Glu Asp Leu Gln Asn Val Gly Met Glu Glu Leu Gly Ala Ala
450 455 460
Leu Met Lys Phe Glu Met Gly Ile Asp Lys Asp Pro Ser Lys Arg Thr
465 470 475 480
Phe Asp Asp Leu Gln Arg Gly Glu Asp Gly Lys Phe Arg Asp Glu Asp
485 490 495
Leu Val Arg Val Leu Lys Glu Ala Met Glu Asp Pro Ala Gly Thr Phe
500 505 510
Gly Ala Arg Met Val Pro Lys Ala Leu Lys Ile Val Glu Ile Met Gly
515 520 525
Ile Lys Gln Ala Arg Ala Trp Gln Val Ala Ser Leu Asn Glu Phe Arg
530 535 540
Asp Phe Phe Gly Leu Lys Arg His Asp Thr Phe Lys Asp Ile Asn Ser
545 550 555 560
Asn Glu Glu Ile Ala Thr Leu Leu Glu Lys Leu Tyr Thr Asp Pro Asp
565 570 575
Met Val Glu Leu Tyr Pro Gly Leu Met Ile Glu Asp Ile Lys Pro Val
580 585 590
Arg Asn Thr Gly Ser Gly Ile Cys Pro Thr Tyr Ser Val Gly Arg Ala
595 600 605
Val Leu Ser Asp Ala Val Thr Leu Val Arg Ser Asp Arg Phe Asn Thr
610 615 620
Ile Asp Tyr Thr Val Ser Asn Leu Thr Ala Trp Gly Tyr Asn Glu Val
625 630 635 640
Gln Gln Asp Tyr Lys Thr Leu Gly Gly Ser Met Leu Tyr Lys Leu Ile
645 650 655
Gln Arg Gly Leu Pro Gly Trp Phe Pro Phe Asn Ser Ile Ala Val Met
660 665 670
Gln Pro Met Tyr Thr Lys Lys Ala Asn Glu Arg Ile Ala Arg Glu Ile
675 680 685
Gly Thr Phe Asn Gln Phe Thr Leu Asp Asp Pro Lys Ala Pro Pro Lys
690 695 700
Pro Val Val Val Ala Ser Ser Glu Gly Ile Lys Arg Val Leu Gly Ser
705 710 715 720
Pro Asp Lys Phe Val Val Pro Trp Leu Thr Pro Leu Asn Ala Leu Tyr
725 730 735
Thr Asp Thr Lys Lys Asp Ile Ser Trp Phe Met Leu Ala Gly Asp Gly
740 745 750
Ser Thr Asn Lys Gln Glu Lys Val Asn Phe Val Asn Ala Met Lys Lys
755 760 765
Val Pro Asn Leu His Asn Ala Val His Gln Phe Ile Glu Arg Val Gly
770 775 780
Arg Gln Leu Ile Glu Lys Glu Thr Phe Lys Leu Lys Glu Gly Leu Cys
785 790 795 800
Gln Met Asp Ile Ile Arg Asp Val Ala Ile Pro Leu Asn Ala Gln Leu
805 810 815
Leu Ala Asp Leu Phe Tyr Phe Asp Leu Arg His Glu Glu Asn Pro Gly
820 825 830
Gly Thr Leu Ser Ala Thr Asp Leu Tyr Arg His Leu Leu Asn Ile Arg
835 840 845
Ile Trp Gly Val Asn Asn Asn Asp Pro Gly Gln Ala Trp Asn Arg Arg
850 855 860
Arg Arg Ala Ala Glu Ser Ala Lys Val Ile Thr Asp Ser Thr Arg Lys
865 870 875 880
Leu Val Asp Glu Val Ser Arg Gly Arg Gly Leu Asn Leu Gly Phe Ile
885 890 895
Ser Ala Ile Asn Glu Val Ala Ser Arg Lys Thr His Ile Lys Lys Asp
900 905 910
Ser Leu Arg Ser Cys Gly Tyr Lys Leu Val Glu Glu Leu Leu Asn Gln
915 920 925
Gly Gly Ser Pro Glu Lys Val Thr Asp Asn Val Trp Leu Thr Ala Phe
930 935 940
Gly Gly Ile Gly Val Pro Val Thr Thr Phe Tyr Glu Val Met Glu Tyr
945 950 955 960
Phe Leu Arg Pro Glu Asn Lys Ser Ile Trp Gly Glu Val Gln Ala Leu
965 970 975
Ala Gln Lys Asn Asp Glu Ala Gly Leu His Ala Tyr Val Asn Glu Ala
980 985 990
Met Arg Leu Thr Ser Gly Gln Arg Asn Val Arg Ile Ala Thr Val Lys
995 10001005
Asp Glu Ile Asp Gly Gln Lys Val Glu Pro Gly Asn Ala Val Val
101010151020
Met Leu Leu Gly Ala Ala Gly Arg Asn Pro Lys Glu Val Pro Asn
102510301035
Ala Asp Lys Phe Asp Ala Lys Arg Ser Thr Asp His Ile Lys Pro
104010451050
Phe Ser Tyr Gly Gln His Glu Cys Val Gly Gln Asp Val Ala Arg
105510601065
Ala Phe Val Thr Gly Leu Val Lys Leu Val Ala Asp Leu Arg Gln
107010751080
Leu Arg Pro Ala Pro Gly Glu Met Gly Lys Val Lys Thr Ile Gln
108510901095
Val Gly Thr Glu Arg Ala Tyr Leu Asn Asp Ser Trp Ser Tyr Leu
110011051110
Gly Phe Asp Ala Ser Thr Trp Lys Val His Phe Asp Gly His Gly
111511201125
Gln Gly Thr Tyr Glu Gly Asp Pro Glu Pro Asn Lys Pro Ile Asp
113011351140
Met Gly Arg Tyr Tyr Tyr Ile Leu Gln Lys Arg Lys Glu Ser Leu
114511501155
Leu Lys Gly
1160
<210> 53
<211> 1251
<212> PRT
<213> 黑曲霉（Aspergillus niger）
<400> 53
Met Gly Ser Ile Phe Ile Ala Leu Leu Leu Cys Leu Leu Ser Tyr Leu
1 5 1015
Tyr Leu Arg Val Phe Ala Asp Asp Leu Thr Arg Pro Leu Gln Ile Leu
202530
Ala Lys Phe Phe Ser Ser His Gln His Pro Thr Trp Lys Leu Arg Pro
354045
Arg Phe Leu Pro Lys Ala Thr Arg Ala Ala Leu Ser Ser Ile Ala Gly
505560
Thr Gly Glu Gly Leu Trp Gln Arg Leu Tyr Ala Arg Thr Phe His Ala
65707580
Gln Glu Leu Ala Glu Val Glu Asp Asp Leu Lys Tyr Gln Ala Gly Glu
859095
Pro Tyr Gly Asp Pro Asp Val Leu Ala Thr Gly Leu Val Lys Asp Leu
100 105 110
Ser Ala Leu Gly Leu Lys Gly Lys Arg Ser Asp Leu Arg Thr Leu Ile
115 120 125
Gln Leu Val Lys Ala Lys Gly Lys Pro Ile Asp Asp Arg Gln Met Leu
130 135 140
Met Glu Lys Val Ile Ala Ile Val Ser Met Leu Pro Arg Thr Ser Lys
145 150 155 160
Ser Arg Gln Arg Leu Thr Gly Ile Leu Val Asp Gln Leu Trp Gln Ser
165 170 175
Leu Glu His Pro Pro Leu Ser Tyr Phe Gly Asn Lys Tyr Gln Tyr Arg
180 185 190
Thr Pro Asp Gly Ser Tyr Asn Asn Pro Leu Glu Pro Asp Leu Gly Lys
195 200 205
Ala Gly Ser Pro Tyr Ala Arg Ser Val Pro Arg Leu Lys Ala Leu His
210 215 220
Gly Val Gln Pro Asp Pro Gly Leu Leu Phe Asp Leu Leu Met Ala Arg
225 230 235 240
Asp Asp Thr Thr Phe Arg Glu Asn Pro Ala Gly Ile Ser Ser Val Leu
245 250 255
Phe Tyr His Ala Ser Ile Ile Ile His Asp Val Phe Cys Thr Asn Arg
260 265 270
Arg Asp Pro Asn Ile Ser Asp Thr Ser Ser Tyr Leu Asp Leu Ala Pro
275 280 285
Leu Tyr Gly Ser Ser Tyr Glu Asp Gln Leu Arg Val Arg Thr Met Gln
290 295 300
Arg Gly Met Leu Lys Pro Asp Thr Phe His Glu Lys Arg Leu Leu Gly
305 310 315 320
Gln Pro Pro Gly Val Asn Val Ile Leu Val Met Tyr Asn Arg Phe His
325 330 335
Asn Tyr Val Ala Asp Val Leu Leu Lys Ile Asn Glu Asn Gly Arg Phe
340 345 350
Thr Leu Pro Pro Thr Thr Ser Glu Asp Ala Lys Arg Lys Ala Leu Ala
355 360 365
Lys Gln Asp Glu Asp Leu Phe Gln Val Thr Arg Leu Ile Val Asn Gly
370 375 380
Leu Tyr Val Asn Ile Ser Leu His Asp Tyr Leu Arg Gly Leu Thr Asn
385 390 395 400
Thr His His Ser Ala Ser Asp Trp Thr Leu Asp Pro Arg Val Ala Val
405 410 415
Ser Arg Ala Phe Asp Ala Asp Gly Val Pro Arg Gly Val Gly Asn Gln
420 425 430
Val Ser Ala Glu Phe Asn Leu Leu Tyr Arg Phe His Ser Val Ile Ser
435 440 445
Arg Arg Asp Glu Gln Trp Thr Asn Glu Phe Leu Lys Ser Leu Phe Pro
450 455 460
Asp Leu Lys Lys Pro Leu Glu Gln Leu Thr Pro Gln Glu Phe Met Gln
465 470 475 480
Gly Leu Ile Asn Tyr Glu Arg Ser Ile Asp Lys Asp Pro Ser Lys Arg
485 490 495
Glu Phe Gly Gly Leu Lys Arg Asn Gln Asp Gly Arg Phe Asn Asp Ala
500 505 510
Glu Leu Val Gln Ile Leu Lys Asp Ser Met Glu Asp Pro Ala Gly Leu
515 520 525
Phe Gly Ala Arg Met Val Pro Lys Ala Leu Arg Met Val Glu Ile Ala
530 535 540
Gly Ile Leu Thr Ala Arg Lys Trp Asn Leu Ala Ser Leu Asn Glu Met
545 550 555 560
Arg Asp Phe Phe Lys Leu Lys Arg His Ser Ser Phe Glu Asp Ile Asn
565 570 575
Pro Asp Pro Lys Ile Ala Asp Leu Leu Arg Lys Leu Tyr Asp His Pro
580 585 590
Asp Met Val Glu Met Tyr Pro Gly Ile Phe Leu Glu Asp Ala Lys Pro
595 600 605
Ala Met Asp Pro Gly Cys Gly Gly Cys Pro Pro Tyr Thr Val Gly Arg
610 615 620
Ala Val Phe Ser Asp Ala Val Thr Leu Val Arg Ser Asp Arg Phe Leu
625 630 635 640
Thr Leu Asp Tyr Thr Ala Ser Asn Leu Thr Asn Trp Gly Ile Arg Glu
645 650 655
Val Gln Gln Asp Tyr Asp Ile Leu Gly Gly Ser Met Phe His Lys Leu
660 665 670
Ile Gln Arg Ala Leu Pro Gly Trp Phe Pro Tyr Asn Ser Leu His Ala
675 680 685
Thr Gln Pro Met Phe Thr Arg Lys Met Asn Glu Gln Ile Ala Lys Glu
690 695 700
Ile Gly Thr Ile Asp Arg Tyr Ser Gln Glu Asp Pro Lys Pro Pro Pro
705 710 715 720
Arg Thr Val Met Leu Ala Asn His Ala Thr Ile Ile Glu Val Leu Lys
725 730 735
Asp Gln Asp Thr Phe Arg Val Pro Trp Ala Arg Tyr Leu Asn Asp Met
740 745 750
Ile Pro Gly Lys Arg Phe Asn Asp Tyr Met Leu Gly Gly Asp Gly Pro
755 760 765
Val Asn Ala Ala Gln Lys Lys Leu Val Lys Ser Ile Leu Phe Ser Pro
770 775 780
Asp Gln Phe Asn Gln Leu Leu Ser Gln Thr Thr Val Arg Leu Gly Lys
785 790 795 800
Glu Leu Leu Glu Leu Asn Thr Leu Gln Leu Ser Lys Asp Leu Asn Gln
805 810 815
Val Asp Ile Ile Arg Asp Val Ala Ile Pro Leu Asn Ala Arg Ile Met
820 825 830
Ala Asp Leu Phe Cys Leu Asp Met Lys Thr Pro Glu Asn Glu Ser Gly
835 840 845
Ser Met Asn Ala Ala Thr Val Tyr Lys His Leu Met Asn Val Arg Thr
850 855 860
Trp Gly Phe Asn Asn Thr Asp Pro Gly Leu Met Leu Gln Arg Arg Lys
865 870 875 880
Trp Ala Ser Glu Ser Ala Glu Ala Leu Val Lys Thr Thr Leu Lys Val
885 890 895
Val Asn Glu Gln Ala Gln Pro Gln Lys Thr His Met Leu Lys Lys Leu
900 905 910
Thr Gly Tyr Gln Arg Ser Glu Val Ser Thr Leu Arg Trp Tyr Gly Asn
915 920 925
Asn Val Val Lys Gln Met Met Glu Met Asp Met Thr Ala Ala Glu Thr
930 935 940
Ala Glu Val Cys Trp Leu Thr Ala Val Gly Gly Val Gly Ala Pro Val
945 950 955 960
Gly Leu Val Ala Asp Val Leu Gln Tyr Tyr Leu Arg Pro Glu Asn Ile
965 970 975
Asp His Trp Lys Arg Ile Gln Asn Leu Val Ser Gln Pro Asp Asn Ser
980 985 990
Gly Ser Ile Asp Lys Leu Leu Arg Gln Tyr Val Leu Glu Ala Gln Arg
995 10001005
Leu Thr Ser Met Glu Cys Thr Val Arg Val Cys Arg Ala His Arg
101010151020
Thr Ile Asn Asp Gln Glu Phe Lys Pro Gly Asp Val Val Ile Thr
102510301035
Leu Leu Gly Pro Ala Cys Arg Asp Pro Thr Ser Ile Pro Asp Ala
104010451050
Glu Thr Phe Lys Leu Asp Arg Pro Ser Asn Ala Tyr Ile His Phe
105510601065
Gly Tyr Gly Ala His Glu Cys Leu Gly Lys Glu Ile Gly Leu Thr
107010751080
Phe Ala Val Ser Met Leu Arg Val Leu Ala Gly Leu Lys Tyr Leu
108510901095
Arg Pro Ala Pro Gly Asp Met Gly Met Leu Lys Ser Ile Ile Val
110011051110
Asp Gly Arg Arg Val Tyr Leu Asn Asp Ser Trp Ser Trp Met Ile
111511201125
Gln Asp Pro Thr Thr Trp Lys Leu His Phe Ala Gly Tyr Gly Gln
113011351140
Gly Ala Tyr Glu Ala Pro Ala Pro Pro Pro Pro Ala Pro Thr Phe
114511501155
Pro Val Ile Asp Gln Ser Pro Asp Thr Ser Asp Glu Asp Gly Val
116011651170
Asp Asp Thr Ile Tyr Ser Thr Val Pro Thr Lys His His His His
117511801185
Gly Asp Pro Glu Tyr Thr Arg Gln Glu Ser Tyr Thr Met Thr Asp
119011951200
Gly Gly Ile Gly Met Ser Thr Gln Tyr Ser Phe Gly Gln His His
120512101215
His His Gln Tyr His Glu Ile Thr Arg Thr Ser Ser Ser Ser Leu
122012251230
Pro Ser Met Leu Gly Asp Asp Glu Ser Tyr Gly Arg Phe Ser Gly
123512401245
Thr Leu Asn
1250
<210> 54
<211> 478
<212> PRT
<213> 马铃薯（Solanum tuberosum）
<400> 54
Met Ser Ser Tyr Ser Glu Leu Ser Asn Leu Pro Ile Arg Glu Ile Pro
1 5 1015
Gly Asp Tyr Gly Phe Pro Ile Ile Ser Ala Ile Lys Asp Arg Tyr Asp
202530
Tyr Phe Tyr Asn Gln Gly Glu Asp Ala Trp Phe His Asn Lys Ala Glu
354045
Lys Tyr Lys Ser Thr Val Val Lys Ile Asn Met Ala Pro Gly Pro Phe
505560
Thr Ser Asn Asp Tyr Lys Leu Val Ala Phe Leu Asp Ala Asn Ser Phe
65707580
Val Cys Met Phe Asp Asn Ser Leu Ile Asp Lys Thr Asp Thr Leu Gly
859095
Gly Thr Phe Lys Pro Gly Lys Glu Tyr Tyr Ser Gly Tyr Arg Pro Val
100 105 110
Ala Phe Ile Asp Thr Lys Asp Pro Asn His Ala Ala Leu Lys Gly Tyr
115 120 125
Ile Leu Ser Ala Phe Ala Lys Arg His Asn Leu Phe Ile Pro Leu Phe
130 135 140
Arg Asn Ser Leu Ser Asp His Leu Phe Asn Asn Leu Glu Lys Gln Val
145 150 155 160
Thr Glu Gln Gly Lys Ser Asp Phe Asn Ala Leu Leu Pro Thr Met Thr
165 170 175
Phe Asn Phe Ile Phe Arg Leu Leu Cys Asp Gln Thr Asn Pro Ser Asp
180 185 190
Thr Val Leu Gly Ala Gln Gly Pro Glu His Leu Arg Lys Trp Leu Phe
195 200 205
Pro Gln Leu Ile Pro Ser Leu Ser Ala Lys Lys Leu Pro Asn Ile Ile
210 215 220
Glu Asp Thr Leu Phe His Asn Phe Leu Ile Pro Phe Gly Phe Ile Lys
225 230 235 240
Ser Asp Tyr Asn Lys Leu Val Asp Ala Phe Ser Lys Ser Ala Val Ser
245 250 255
Ile Leu Asp Glu Ala Glu Lys Leu Gly Ile Lys Arg Glu Glu Ala Val
260 265 270
Gln Asn Ile Leu Phe Leu Val Gly Ile Asn Met Phe Ala Gly Leu Asn
275 280 285
Ala Phe Ser Pro His Leu Phe Arg Phe Val Gly Glu Ala Gly Ala Ser
290 295 300
Leu His Thr Gln Leu Ala Lys Glu Ile Arg Thr Val Ile Lys Glu Glu
305 310 315 320
Gly Gly Ala Ile Thr Leu Ser Ala Ile Asn Lys Met Ser Leu Val Lys
325 330 335
Ser Val Val Tyr Glu Thr Leu Arg Leu Arg Pro Pro Val Pro Leu Gln
340 345 350
Tyr Gly Lys Ala Lys Lys Asp Phe Met Val Gln Ser His Asp Ala Ser
355 360 365
Tyr Lys Ile Asn Lys Gly Gln Phe Val Val Gly Tyr Gln Pro Met Ala
370 375 380
Ser Arg Asp Pro Lys Ile Phe Ala Asn Pro Asp Glu Phe Val Pro Asp
385 390 395 400
Arg Phe Met Asn Asp Gly Glu Lys Met Leu Lys His Val Leu Trp Ser
405 410 415
Asn Gly Arg Glu Thr Glu Asn Pro Ala Pro Asp Asn Lys Gln Cys Pro
420 425 430
Gly Lys Asp Leu Val His Leu Leu Gly Arg Leu Ile Leu Val Glu Phe
435 440 445
Phe Met Arg Tyr Asp Thr Phe Thr Val Glu Ile Thr Pro Leu Phe Arg
450 455 460
Ala Pro Asn Val Ala Phe Lys Thr Leu Thr Lys Ala Ser Lys
465 470 475
<210> 55
<211> 472
<212> PRT
<213> 大蒜（Allium sativum）
<400> 55
Met Ser Thr Ser Asn Gly Ser Thr Glu Asn Ile Gln Lys Pro Leu Arg
1 5 1015
Lys Ile Pro Asp Ile Thr Gly Thr Pro Ile Leu Thr Ala Ile Lys Asp
202530
Arg Leu Asp Phe Phe Tyr Asn Gln Gly Gln Tyr Glu Tyr Phe Gln Ser
354045
Arg Val Lys Lys Asn Asn Ser Thr Ile Leu Arg Met Asn Met Ile Pro
505560
Gly Pro Phe Ala Ser Asn Pro Lys Ile Val Ala Leu Cys Asp Ala Ala
65707580
Ser Phe Pro Thr Leu Phe Asp Pro Ser Lys Val Ser Lys Val Asn Ser
859095
Leu Thr Gly Asn Tyr Met Pro Ala Leu Ser Phe Thr Gly Gly Tyr Arg
100 105 110
Val Cys Ala Tyr Leu Asp Pro Ser Glu Pro Thr His Thr Lys Ile Lys
115 120 125
Gln Val Phe Phe Asn Ala Gln Ala Ala Lys Lys Asp Thr Phe Ile Pro
130 135 140
Thr Phe Val Ser Thr Phe Asn Ser Met Phe Asp Lys Met Asp Ala Glu
145 150 155 160
Val Glu Ser Lys Lys Lys Ala Glu Phe Thr Lys Phe Asn Glu Ala Ala
165 170 175
Val Phe Glu Phe Val Gly Leu Ala Leu Val Gly Pro Lys Pro Ala Arg
180 185 190
Glu Val Phe Asp Ser Ala Lys Lys Ser Val Phe Phe Gln Phe His Pro
195 200 205
Phe Ile Thr Ala Gly Leu Pro Ala Leu Val Glu Glu Leu Ala Phe His
210 215 220
Met Phe Pro Phe Pro Ser Phe Val Ala Lys Ser Ser Tyr Lys Ile Leu
225 230 235 240
Tyr Glu Tyr Phe Ser Thr Gly Gly Ser Trp Ile Leu Asp Asn Ala Glu
245 250 255
Glu Ile Gly Leu Ser Arg Glu Glu Ala Ile His His Leu Ile Phe Thr
260 265 270
Trp Ala Ile Asn Ala Tyr Leu Gly Ile Arg Thr Cys Leu Met Arg Leu
275 280 285
Phe Lys Trp Ile Val Ala Ser Gly Pro Asp Leu Gln Glu Lys Leu Ala
290 295 300
Arg Glu Val Arg Ser Val Val Arg Ser Glu Glu Gly Lys Ile Thr Phe
305 310 315 320
Ala Gly Ile Glu Lys Met Glu Leu Val Lys Ser Val Ala Tyr Glu Ser
325 330 335
Phe Arg Phe Asp Pro Pro Val Gln Val Gln Tyr Gly Thr Ala Lys Ser
340 345 350
Asp Leu Ile Ile Glu Ser His Asp Gly Lys Tyr Gln Val Lys Lys Gly
355 360 365
Glu Met Leu Cys Gly Phe Gln Pro Met Ala Thr Arg Asp Pro Lys Val
370 375 380
Phe Asp Arg Ala Asp Glu Phe Val Pro Asp Arg Phe Met Gly Asp Gly
385 390 395 400
Lys Lys Leu Val Lys His Val Leu Trp Ala Asn Gly Tyr Gly Thr Asp
405 410 415
Ala Pro Lys Ala Asp Asp Lys Ile Cys Ala Gly Lys Asp Leu Gly Val
420 425 430
Leu Val Gly Arg Leu Leu Ile Ala Val Met Phe Leu Arg Tyr Asp Lys
435 440 445
Ile Gly Gly Val Val Gly Lys Thr Met Glu Glu Val Asp Val Ile Val
450 455 460
Asn Glu Leu Thr Lys Val Ala Val
465 470
<210> 56
<211> 1081
<212> PRT
<213> Asperigullus nidalus
<400> 56
Met Gly Glu Asp Lys Glu Thr Asn Ile Leu Ala Gly Leu Gly Asn Thr
1 5 1015
Ile Ser Gln Val Glu Asn Val Val Ala Ala Ser Leu Arg Pro Leu Pro
202530
Thr Ala Thr Gly Asp Gly Thr Tyr Val Ala Glu Ser Thr Gln Thr Gly
354045
Leu Ala Lys Asp Leu Ser His Val Asp Leu Lys Asp Val Arg Thr Leu
505560
Ala Glu Val Val Lys Ser Ala Ala Thr Gly Glu Pro Val Asp Asp Lys
65707580
Gln Tyr Ile Met Glu Arg Val Ile Gln Leu Ala Ala Gly Leu Pro Ser
859095
Thr Ser Arg Asn Ala Ala Glu Leu Thr Lys Ser Phe Leu Asn Met Leu
100 105 110
Trp Asn Asp Leu Glu His Pro Pro Val Ser Tyr Leu Gly Ala Asp Ser
115 120 125
Met His Arg Lys Ala Asp Gly Ser Gly Asn Asn Arg Phe Trp Pro Gln
130 135 140
Leu Gly Ala Ala Gly Ser Ala Tyr Ala Arg Ser Val Arg Pro Lys Thr
145 150 155 160
Met Gln Ser Pro Ser Leu Pro Asp Pro Glu Thr Ile Phe Asp Cys Leu
165 170 175
Leu Arg Arg Lys Glu Tyr Arg Glu His Pro Asn Lys Ile Ser Ser Val
180 185 190
Leu Phe Tyr Leu Ala Ser Ile Ile Ile His Asp Leu Phe Gln Thr Asp
195 200 205
Pro Lys Asp Asn Ser Val Ser Lys Thr Ser Ser Tyr Leu Asp Leu Ser
210 215 220
Pro Leu Tyr Gly Asn Asn Gln Asp Glu Gln Asn Leu Val Arg Thr Phe
225 230 235 240
Lys Asp Gly Lys Leu Lys Pro Asp Cys Phe Ala Thr Lys Arg Val Leu
245 250 255
Gly Phe Pro Pro Gly Val Gly Val Leu Leu Ile Met Phe Asn Arg Phe
260 265 270
His Asn Tyr Val Val Asp Gln Leu Ala Ala Ile Asn Glu Cys Gly Arg
275 280 285
Phe Thr Lys Pro Asp Glu Ser Asn Val Asp Glu Tyr Ala Lys Tyr Asp
290 295 300
Asn Asn Leu Phe Gln Thr Gly Arg Leu Val Thr Cys Gly Leu Tyr Ala
305 310 315 320
Asn Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg
325 330 335
Thr Asp Ser Thr Trp Ser Leu Asp Pro Arg Met Glu Met Lys Asp Gly
340 345 350
Leu Leu Gly Glu Ala Ala Ala Met Ala Thr Gly Asn Gln Val Ser Ala
355 360 365
Glu Phe Asn Val Val Tyr Arg Trp His Ala Cys Ile Ser Lys Arg Asp
370 375 380
Glu Lys Trp Thr Glu Asp Phe His Arg Glu Ile Met Pro Gly Val Asp
385 390 395 400
Pro Ser Thr Leu Ser Met Gln Asp Phe Val Ala Gly Leu Gly Arg Trp
405 410 415
Gln Ala Gly Leu Pro Gln Glu Pro Leu Glu Arg Pro Phe Ser Gly Leu
420 425 430
Gln Arg Lys Pro Asp Gly Ala Phe Asn Asp Asp Asp Leu Val Asn Leu
435 440 445
Phe Glu Lys Ser Val Glu Asp Cys Ala Gly Ala Phe Gly Ala Ser His
450 455 460
Val Pro Ala Ile Phe Lys Ser Val Glu Ala Leu Gly Ile Met Gln Ala
465 470 475 480
Arg Arg Trp Asn Leu Gly Thr Leu Asn Glu Phe Arg Gln Tyr Phe Asn
485 490 495
Leu Ala Pro His Lys Thr Phe Glu Asp Ile Asn Ser Asp Pro Tyr Ile
500 505 510
Ala Asp Gln Leu Lys Arg Leu Tyr Asp His Pro Asp Leu Val Glu Ile
515 520 525
Tyr Pro Gly Val Val Val Glu Glu Ala Lys Asp Ser Met Val Pro Gly
530 535 540
Ser Gly Leu Cys Thr Asn Phe Thr Ile Ser Arg Ala Ile Leu Ser Asp
545 550 555 560
Ala Val Ala Leu Val Arg Gly Asp Arg Phe Tyr Thr Val Asp Tyr Thr
565 570 575
Pro Lys His Leu Thr Asn Trp Ala Tyr Asn Glu Ile Gln Pro Asn Asn
580 585 590
Ala Val Asp Gln Gly Gln Val Phe Tyr Lys Leu Val Leu Arg Ala Phe
595 600 605
Pro Asn His Phe Asp Gly Asn Ser Ile Tyr Ala His Phe Pro Leu Val
610 615 620
Val Pro Ser Glu Asn Glu Lys Ile Leu Lys Ser Leu Gly Val Ala Glu
625 630 635 640
Lys Tyr Ser Trp Glu Lys Pro Ser Arg Ile Ser His Pro Ile Phe Ile
645 650 655
Ser Ser His Ala Ala Cys Met Ser Ile Leu Glu Asn Gln Glu Thr Phe
660 665 670
Lys Val Thr Trp Gly Arg Lys Ile Glu Phe Leu Met Gln Arg Asp Lys
675 680 685
His Gln Tyr Gly Lys Asp Phe Met Leu Ser Gly Asp Arg Pro Pro Asn
690 695 700
Ala Ala Ser Arg Lys Met Met Gly Ser Ala Leu Tyr Arg Asp Glu Trp
705 710 715 720
Glu Ala Glu Val Lys Asn Phe Tyr Glu Gln Thr Thr Leu Lys Leu Leu
725 730 735
His Lys Asn Ser Tyr Lys Leu Ala Gly Val Asn Gln Val Asp Ile Val
740 745 750
Arg Asp Val Ala Asn Leu Ala Gln Val His Phe Cys Ser Ser Val Phe
755 760 765
Ser Leu Pro Leu Lys Thr Asp Ser Asn Pro Arg Gly Ile Phe Ala Glu
770 775 780
Ser Glu Leu Tyr Lys Ile Met Ala Ala Val Phe Thr Ala Ile Phe Tyr
785 790 795 800
Asp Ala Asp Ile Gly Lys Ser Phe Glu Leu Asn Gln Ala Ala Arg Thr
805 810 815
Val Thr Gln Gln Leu Gly Gln Leu Thr Met Ala Asn Val Glu Ile Ile
820 825 830
Ala Lys Thr Gly Leu Ile Ala Asn Leu Val Asn Arg Leu His Arg Arg
835 840 845
Asp Val Leu Ser Glu Tyr Gly Ile His Met Ile Gln Arg Leu Leu Asp
850 855 860
Ser Gly Leu Pro Ala Thr Glu Ile Val Trp Thr His Ile Leu Pro Thr
865 870 875 880
Ala Gly Gly Met Val Ala Asn Gln Ala Gln Leu Phe Ser Gln Cys Leu
885 890 895
Asp Tyr Tyr Leu Ser Glu Glu Gly Ser Gly His Leu Pro Glu Ile Asn
900 905 910
Arg Leu Ala Lys Glu Asn Thr Pro Glu Ala Asp Glu Leu Leu Thr Arg
915 920 925
Tyr Phe Met Glu Gly Ala Arg Leu Arg Ser Ser Val Ala Leu Pro Arg
930 935 940
Val Ala Ala Gln Pro Thr Val Val Glu Asp Asn Gly Glu Lys Leu Thr
945 950 955 960
Ile Lys Ala Gly Gln Val Val Met Cys Asn Leu Val Ser Ala Cys Met
965 970 975
Asp Pro Thr Ala Phe Pro Asp Pro Glu Lys Val Lys Leu Asp Arg Asp
980 985 990
Met Asn Leu Tyr Ala His Phe Gly Phe Gly Pro His Lys Cys Leu Gly
995 10001005
Leu Asp Leu Cys Lys Thr Gly Leu Ser Thr Met Leu Lys Val Leu
101010151020
Gly Arg Leu Asp Asn Leu Arg Arg Ala Pro Gly Ala Gln Gly Gln
102510301035
Leu Lys Lys Leu Ser Gly Pro Gly Gly Ile Ala Lys Tyr Met Asn
104010451050
Glu Asp Gln Ser Gly Phe Thr Pro Phe Pro Ser Thr Met Lys Ile
105510601065
Gln Trp Asp Gly Glu Leu Pro Gln Leu Lys Glu Asp Phe
107010751080
<210> 57
<211> 1079
<212> PRT
<213> 烟曲霉（Aspergillus fumigatus）
<400> 57
Met Ser Glu Lys Gln Thr Gly Ser Ala Asn Gly Gly Leu Gly Lys Thr
1 5 1015
Leu Ala Gln Leu Glu Gln Val Val Ser Ala Ser Leu Arg Pro Leu Pro
202530
Cys Gln Thr Gly Asp Gly Thr Tyr Val Thr Glu Gln Val Lys Thr Gly
354045
Ile Leu Lys Asp Leu Ser His Val Asp Leu Gly Asp Leu Lys Thr Leu
505560
Val Asp Val Ser Lys Ser Ala Leu Thr Gly Glu Ala Leu Asp Asp Arg
65707580
Lys Tyr Ile Met Glu Arg Val Ile Gln Leu Ser Ala Gly Leu Pro Ser
859095
Thr Ser Gln Ile Gly Lys Glu Leu Thr Asn Thr Phe Leu Thr Thr Leu
100 105 110
Trp Asn Asp Leu Glu His Pro Pro Ile Ser Tyr Leu Gly Arg Asp Ala
115 120 125
Met Tyr Arg Arg Ala Asp Gly Ser Gly Asn Asn Val Leu Trp Pro His
130 135 140
Ile Gly Ala Ala Gly Thr Pro Tyr Ala Arg Ser Val Gln Pro Lys Thr
145 150 155 160
Val Gln Ser Pro Asn Leu Pro Asp Pro Glu Thr Leu Phe Asp Cys Leu
165 170 175
Leu Ala Arg Lys Glu Tyr Lys Glu His Pro Asn Lys Ile Ser Ser Val
180 185 190
Leu Phe Tyr Ile Ala Ser Ile Ile Ile His Asp Leu Phe Glu Thr Asp
195 200 205
Arg Lys Asp Pro Ala Ile Ser Leu Thr Ser Ser Tyr Leu Asp Leu Ser
210 215 220
Pro Leu Tyr Gly Asn Asn Gln Gln Glu Gln Asp Leu Ile Arg Thr Phe
225 230 235 240
Lys Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Thr Lys Arg Val Leu
245 250 255
Gly Phe Pro Pro Gly Val Gly Val Val Leu Ile Met Phe Asn Arg Phe
260 265 270
His Asn Tyr Val Val Glu Lys Leu Ala Met Ile Asn Glu Gly Gly Arg
275 280 285
Phe Thr Lys Pro Gln Glu Ser Asp Thr Ala Ala Tyr Ala Lys Tyr Asp
290 295 300
Asn Asp Leu Phe Gln Thr Gly Arg Leu Val Thr Cys Gly Leu Tyr Val
305 310 315 320
Asn Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg
325 330 335
Thr Asp Ser Ile Trp Ser Leu Asp Pro Arg Ser Glu Met Lys Asp Gly
340 345 350
Leu Leu Gly Arg Ala Ala Ala Gln Ala Thr Gly Asn Gln Val Ala Ala
355 360 365
Glu Phe Asn Leu Val Tyr Arg Trp His Ser Cys Ile Ser Gln Arg Asp
370 375 380
Gln Lys Trp Thr Glu Asp Met Tyr Gln Glu Leu Phe Pro Gly Gln Asp
385 390 395 400
Pro Ser Lys Ile Ser Leu Gln Asp Phe Leu Arg Gly Leu Gly Arg Trp
405 410 415
Glu Ala Lys Leu Pro Gly Glu Pro Arg Glu Arg Pro Phe Ala Gly Leu
420 425 430
Gln Arg Lys Ala Asp Gly Ser Tyr Asp Asp Asn Asp Leu Val Lys Ile
435 440 445
Phe Glu Glu Ser Val Glu Asp Cys Ala Gly Ala Phe Gly Ala Leu His
450 455 460
Val Pro Thr Val Phe Arg Ser Ile Glu Ala Leu Gly Ile Gln Gln Ala
465 470 475 480
Arg Ser Trp Asn Leu Ala Thr Leu Asn Glu Phe Arg Lys Tyr Phe Asn
485 490 495
Leu Ala Pro Tyr Lys Thr Phe Glu Glu Ile Asn Ser Asp Pro Tyr Val
500 505 510
Ala Asp Gln Leu Lys Arg Leu Tyr Asp His Pro Asp Arg Val Glu Ile
515 520 525
Tyr Pro Gly Ile Ile Val Glu Asp Ala Lys Glu Ser Met Ala Pro Gly
530 535 540
Ser Gly Leu Cys Thr Asn Phe Thr Ile Ser Arg Ala Ile Leu Ser Asp
545 550 555 560
Ala Val Ala Leu Val Arg Gly Asp Arg Phe His Thr Val Asp Phe Thr
565 570 575
Pro Lys His Leu Thr Asn Trp Ala Tyr Asn Glu Ile Gln Pro Gln Asp
580 585 590
Ser Val Asp Gln Thr His Val Phe Tyr Lys Leu Val Leu Arg Ala Phe
595 600 605
Pro Asn His Phe Arg Gly Asp Ser Ile Tyr Ala His Phe Pro Leu Val
610 615 620
Val Pro Ser Glu Asn Lys Lys Ile Leu Thr Lys Leu Gly Thr Ala Asp
625 630 635 640
Lys Tyr Ser Trp Asp Arg Pro Asn Tyr Thr Pro Pro Pro Gln Phe Ile
645 650 655
Asn Ser His Ser Ala Cys Met Ser Ile Leu Ser Asp Gln Glu Thr Phe
660 665 670
Lys Val Thr Trp Gly Ser Lys Ile Glu Phe Leu Met Arg His Asn Asn
675 680 685
Gln Pro Tyr Gly Arg Asp Phe Met Leu Ser Gly Asp Arg Thr Pro Asn
690 695 700
Ala Met Ser Arg Gln Met Met Gly Lys Ala Leu Tyr Arg Asp Lys Trp
705 710 715 720
Glu Thr Glu Val Lys Arg Phe Tyr Glu Asn Ile Thr Leu Lys Leu Leu
725 730 735
His Arg Tyr Ser Tyr Lys Leu Ala Gly Val Asn Gln Val Asp Val Val
740 745 750
Arg Asp Ile Ala Asn Leu Ala Gln Val His Phe Cys Ala Ser Val Phe
755 760 765
Ser Leu Pro Leu Lys Thr Glu Ser Asn Pro Arg Gly Ile Phe Thr Glu
770 775 780
Ser Glu Leu Tyr Gln Ile Met Ala Val Val Phe Thr Ser Ile Phe Tyr
785 790 795 800
Asp Ala Asp Ile Gly Lys Ser Phe Glu Leu Asn Gln Ala Ala Arg Ala
805 810 815
Val Thr Gln Gln Leu Gly Gln Leu Thr Leu Ala Asn Val Glu Leu Ile
820 825 830
Ala Lys Thr Gly Phe Ile Ala Asn Leu Val Asn Ser Leu His Arg His
835 840 845
Asp Val Leu Ser Glu Tyr Gly Val His Met Ile Gln Arg Leu Leu Asp
850 855 860
Ser Gly Met Pro Ala Pro Glu Ile Val Trp Thr His Val Leu Pro Thr
865 870 875 880
Ala Gly Gly Met Val Ala Asn Gln Ala Gln Leu Phe Ser Gln Ser Leu
885 890 895
Asp Tyr Tyr Leu Ser Glu Glu Gly Ser Val His Leu Pro Glu Ile Asn
900 905 910
Arg Leu Ala Lys Glu Asp Thr Thr Glu Ala Asp Asp Leu Leu Leu Arg
915 920 925
Tyr Phe Met Glu Gly Ala Arg Ile Arg Ser Ser Val Ala Leu Pro Arg
930 935 940
Val Val Ala Gln Pro Thr Val Val Glu Asp Asn Gly Gln Lys Ile Thr
945 950 955 960
Leu Lys Gln Gly Gln His Ile Ile Cys Asn Leu Val Ser Ala Ser Met
965 970 975
Asp Pro Val Thr Phe Pro Glu Pro Asp Lys Val Lys Leu Asp Arg Asp
980 985 990
Met Asn Leu Tyr Ala His Phe Gly Phe Gly Pro His Gln Cys Leu Gly
995 10001005
Leu Gly Leu Cys Lys Thr Ala Leu Thr Thr Met Leu Lys Val Ile
101010151020
Gly Arg Leu Asp Asn Leu Arg Arg Ala Pro Gly Gly Gln Gly Lys
102510301035
Leu Lys Lys Leu Ser Gly Pro Gly Gly Ile Ala Met Tyr Met Thr
104010451050
Pro Asp Gln Thr Ala Phe Phe Pro Phe Pro Thr Thr Met Lys Ile
105510601065
Gln Trp Asp Gly Asp Leu Pro Glu Val Lys Glu
10701075
<210> 58
<211> 1153
<212> PRT
<213> 稻瘟病菌（Magnaporthe oryzae）
<400> 58
Met Asp Gly Ala Val Arg Leu Asp Trp Thr Gly Leu Asp Leu Thr Gly
1 5 1015
His Glu Ile His Asp Gly Val Pro Ile Ala Ser Arg Val Gln Val Met
202530
Val Ser Phe Pro Leu Phe Lys Asp Gln His Ile Ile Met Ser Ser Lys
354045
Glu Ser Pro Ser Arg Lys Ser Ser Thr Ile Gly Gln Ser Thr Arg Asn
505560
Gly Ser Cys Gln Ala Asp Thr Gln Lys Gly Gln Leu Pro Pro Val Gly
65707580
Glu Lys Pro Lys Pro Val Lys Glu Asn Pro Met Lys Lys Leu Lys Glu
859095
Met Ser Gln Arg Pro Leu Pro Thr Gln His Gly Asp Gly Thr Tyr Pro
100 105 110
Thr Glu Lys Lys Leu Thr Gly Ile Gly Glu Asp Leu Lys His Ile Arg
115 120 125
Gly Tyr Asp Val Lys Thr Leu Leu Ala Met Val Lys Ser Lys Leu Lys
130 135 140
Gly Glu Lys Leu Lys Asp Asp Lys Thr Met Leu Met Glu Arg Val Met
145 150 155 160
Gln Leu Val Ala Arg Leu Pro Thr Glu Ser Lys Lys Arg Ala Glu Leu
165 170 175
Thr Asp Ser Leu Ile Asn Glu Leu Trp Glu Ser Leu Asp His Pro Pro
180 185 190
Leu Asn Tyr Leu Gly Pro Glu His Ser Tyr Arg Thr Pro Asp Gly Ser
195 200 205
Tyr Asn His Pro Phe Asn Pro Gln Leu Gly Ala Ala Gly Ser Arg Tyr
210 215 220
Ala Arg Ser Val Ile Pro Thr Val Thr Pro Pro Gly Ala Leu Pro Asp
225 230 235 240
Pro Gly Leu Ile Phe Asp Ser Ile Met Gly Arg Thr Pro Asn Ser Tyr
245 250 255
Arg Lys His Pro Asn Asn Val Ser Ser Ile Leu Trp Tyr Trp Ala Thr
260 265 270
Ile Ile Ile His Asp Ile Phe Trp Thr Asp Pro Arg Asp Ile Asn Thr
275 280 285
Asn Lys Ser Ser Ser Tyr Leu Asp Leu Ala Pro Leu Tyr Gly Asn Ser
290 295 300
Gln Glu Met Gln Asp Ser Ile Arg Thr Phe Lys Asp Gly Arg Met Lys
305 310 315 320
Pro Asp Cys Tyr Ala Asp Lys Arg Leu Ala Gly Met Pro Pro Gly Val
325 330 335
Ser Val Leu Leu Ile Met Phe Asn Arg Phe His Asn His Val Ala Glu
340 345 350
Asn Leu Ala Leu Ile Asn Glu Gly Gly Arg Phe Asn Lys Pro Ser Asp
355 360 365
Leu Leu Glu Gly Glu Ala Arg Glu Ala Ala Trp Lys Lys Tyr Asp Asn
370 375 380
Asp Leu Phe Gln Val Ala Arg Leu Val Thr Ser Gly Leu Tyr Ile Asn
385 390 395 400
Ile Thr Leu Val Asp Tyr Val Arg Asn Ile Val Asn Leu Asn Arg Val
405 410 415
Asp Thr Thr Trp Thr Leu Asp Pro Arg Gln Asp Ala Gly Ala His Val
420 425 430
Gly Thr Ala Asp Gly Ala Glu Arg Gly Thr Gly Asn Ala Val Ser Ala
435 440 445
Glu Phe Asn Leu Cys Tyr Arg Trp His Ser Cys Ile Ser Glu Lys Asp
450 455 460
Ser Lys Phe Val Glu Ala Gln Phe Gln Asn Ile Phe Gly Lys Pro Ala
465 470 475 480
Ser Glu Val Arg Pro Asp Glu Met Trp Lys Gly Phe Ala Lys Met Glu
485 490 495
Gln Asn Thr Pro Ala Asp Pro Gly Gln Arg Thr Phe Gly Gly Phe Lys
500 505 510
Arg Gly Pro Asp Gly Lys Phe Asp Asp Asp Asp Leu Val Arg Cys Ile
515 520 525
Ser Glu Ala Val Glu Asp Val Ala Gly Ala Phe Gly Ala Arg Asn Val
530 535 540
Pro Gln Ala Met Lys Val Val Glu Thr Met Gly Ile Ile Gln Gly Arg
545 550 555 560
Lys Trp Asn Val Ala Gly Leu Asn Glu Phe Arg Lys His Phe His Leu
565 570 575
Lys Pro Tyr Ser Thr Phe Glu Asp Ile Asn Ser Asp Pro Gly Val Ala
580 585 590
Glu Ala Leu Arg Arg Leu Tyr Asp His Pro Asp Asn Val Glu Leu Tyr
595 600 605
Pro Gly Leu Val Ala Glu Glu Asp Lys Gln Pro Met Val Pro Gly Val
610 615 620
Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Val Val Leu Ser Asp Ala
625 630 635 640
Val Cys Leu Val Arg Gly Asp Arg Phe Tyr Thr Thr Asp Phe Thr Pro
645 650 655
Arg Asn Leu Thr Asn Trp Gly Tyr Lys Glu Val Asp Tyr Asp Leu Ser
660 665 670
Val Asn His Gly Cys Val Phe Tyr Lys Leu Phe Ile Arg Ala Phe Pro
675 680 685
Asn His Phe Lys Gln Asn Ser Val Tyr Ala His Tyr Pro Met Val Val
690 695 700
Pro Ser Glu Asn Lys Arg Ile Leu Glu Ala Leu Gly Arg Ala Asp Leu
705 710 715 720
Phe Asp Phe Glu Ala Pro Lys Tyr Ile Pro Pro Arg Val Asn Ile Thr
725 730 735
Ser Tyr Gly Gly Ala Glu Tyr Ile Leu Glu Thr Gln Glu Lys Tyr Lys
740 745 750
Val Thr Trp His Glu Gly Leu Gly Phe Leu Met Gly Glu Gly Gly Leu
755 760 765
Lys Phe Met Leu Ser Gly Asp Asp Pro Leu His Ala Gln Gln Arg Lys
770 775 780
Cys Met Ala Ala Gln Leu Tyr Lys Asp Gly Trp Thr Glu Ala Val Lys
785 790 795 800
Ala Phe Tyr Ala Gly Met Met Glu Glu Leu Leu Val Ser Lys Ser Tyr
805 810 815
Phe Leu Gly Asn Asn Lys His Arg His Val Asp Ile Ile Arg Asp Val
820 825 830
Gly Asn Met Val His Val His Phe Ala Ser Gln Val Phe Gly Leu Pro
835 840 845
Leu Lys Thr Ala Lys Asn Pro Thr Gly Val Phe Thr Glu Gln Glu Met
850 855 860
Tyr Gly Ile Leu Ala Ala Ile Phe Thr Thr Ile Phe Phe Asp Leu Asp
865 870 875 880
Pro Ser Lys Ser Phe Pro Leu Arg Thr Lys Thr Arg Glu Val Cys Gln
885 890 895
Lys Leu Ala Lys Leu Val Glu Ala Asn Val Lys Leu Ile Asn Lys Ile
900 905 910
Pro Trp Ser Arg Gly Met Phe Val Gly Lys Pro Ala Lys Asp Glu Pro
915 920 925
Leu Ser Ile Tyr Gly Lys Thr Met Ile Lys Gly Leu Lys Ala His Gly
930 935 940
Leu Ser Asp Tyr Asp Ile Ala Trp Ser His Val Val Pro Thr Ser Gly
945 950 955 960
Ala Met Val Pro Asn Gln Ala Gln Val Phe Ala Gln Ala Val Asp Tyr
965 970 975
Tyr Leu Ser Pro Ala Gly Met His Tyr Ile Pro Glu Ile His Met Val
980 985 990
Ala Leu Gln Pro Ser Thr Pro Glu Thr Asp Ala Leu Leu Leu Gly Tyr
995 10001005
Ala Met Glu Gly Ile Arg Leu Ala Gly Thr Phe Gly Ser Tyr Arg
101010151020
Glu Ala Ala Val Asp Asp Val Val Lys Glu Asp Asn Gly Arg Gln
102510301035
Val Pro Val Lys Ala Gly Asp Arg Val Phe Val Ser Phe Val Asp
104010451050
Ala Ala Arg Asp Pro Lys His Phe Pro Asp Pro Glu Val Val Asn
105510601065
Pro Arg Arg Pro Ala Lys Lys Tyr Ile His Tyr Gly Val Gly Pro
107010751080
His Ala Cys Leu Gly Arg Asp Ala Ser Gln Ile Ala Ile Thr Glu
108510901095
Met Phe Arg Cys Leu Phe Arg Arg Arg Asn Val Arg Arg Val Pro
110011051110
Gly Pro Gln Gly Glu Leu Lys Lys Val Pro Arg Pro Gly Gly Phe
111511201125
Tyr Val Tyr Met Arg Glu Asp Trp Gly Gly Leu Phe Pro Phe Pro
113011351140
Val Thr Met Arg Val Met Trp Asp Asp Glu
11451150
<210> 59
<211> 1161
<212> PRT
<213> 苹果炭疽病菌（Glomerella cingulate）
<400> 59
Met Ala Phe Asn Thr Lys Phe Gln Ala Gly Glu Ser Tyr Gly Asp Glu
1 5 1015
Arg Lys Gly Ser Asn Asn Phe Phe Ala Asp Pro Lys Lys Val Ala Met
202530
Asp Ile Ile Lys Asp Leu Gly Gly Ala His Gly Gln Leu Asn Leu Leu
354045
Glu Val Thr Ala Leu Val Gln Gln Leu Leu Gln Lys Gly Glu Pro Leu
505560
Asp Asp Lys Lys Gly Thr Thr Glu Ala Leu Ile Gly Ile Leu Thr Ser
65707580
Leu Pro Ser Gly Ser Asn Thr Arg Val Gln Leu Thr Asn Lys Leu Ile
859095
Asp Thr Leu Trp Gly Asn Leu Gln His Pro Pro Leu Ser Tyr Val Gly
100 105 110
Gly Asp Val Lys Tyr Glu Val Val Lys Thr Lys Glu Gln Leu Ala Lys
115 120 125
Glu Gln Ala Asp Leu Ala Ala Gln Gly Lys Ala Pro Gln Ser Pro Glu
130 135 140
Glu Ser His Ile Thr Phe Lys Ala Pro Asp Phe Pro Asp Ile Thr Leu
145 150 155 160
Arg Glu His Leu Pro Thr Pro Pro Asp Asn Tyr Lys Met Pro Asp Gly
165 170 175
Ser Tyr Asn Asn Ile Leu Glu Pro Asn Leu Gly Ala Ala Gly Thr Pro
180 185 190
Tyr Ala Lys Thr Val Lys Thr Glu Lys Arg Leu Ala Gly Val Lys Pro
195 200 205
Asp Pro Gly Leu Leu Phe Asp Leu Leu Leu Ala Arg Asp Asp Lys Lys
210 215 220
Phe Thr Glu Asn Pro Ala Gly Ile Ser Ser Met Leu Phe Tyr His Ala
225 230 235 240
Ser Ile Ile Ile His Asp Ile Phe Arg Thr Asn Arg His Asp Leu Asn
245 250 255
Lys Ser Asp Thr Ser Ser Tyr Leu Asp Leu Ala Pro Leu Tyr Gly Ser
260 265 270
Ser Phe Lys Asp Gln Leu Glu Ile Arg Thr Met Lys Glu Gly Lys Leu
275 280 285
Lys Pro Asp Thr Phe His Glu Lys Arg Leu Leu Gly Gln Pro Ala Gly
290 295 300
Val Asn Val Met Leu Val Leu Ser Ser Arg Phe His Asn Tyr Val Ala
305 310 315 320
Asp Ile Leu Leu Lys Ile Asn Glu Asn Gly Arg Phe Thr Leu Gln Thr
325 330 335
Ala Lys Asp Ala Ala Pro Glu Asp Gln Ala Lys Ala Val Ala Lys Gln
340 345 350
Asp His Asp Leu Phe Asn Val Ala Arg Leu Val Thr Gly Gly Leu Tyr
355 360 365
Ile Asn Ile Cys Leu His Asp Tyr Leu Arg Ala Ile Thr Asn Thr His
370 375 380
His Ser Lys Ser Asp Trp Thr Leu Asp Pro Arg Val Glu Ile Gly Lys
385 390 395 400
Gln Phe Asp Gly Glu Gly Val Pro Arg Gly Val Gly Asn Gln Val Ser
405 410 415
Val Glu Phe Asn Leu Leu Tyr Arg Phe His Ser Cys Ile Ser Lys Lys
420 425 430
Asp Glu Arg Trp Ile Asp Gly Phe Phe Ala Lys Leu Phe Pro Gly Arg
435 440 445
Lys Pro Glu Asp Leu Gln Asn Val Gly Met Glu Glu Leu Gly Ala Ala
450 455 460
Leu Met Lys Phe Glu Met Gly Ile Asp Lys Asp Pro Ser Lys Arg Thr
465 470 475 480
Phe Asp Asp Leu Gln Arg Gly Glu Asp Gly Lys Phe Arg Asp Glu Asp
485 490 495
Leu Val Arg Val Leu Lys Glu Ala Met Glu Asp Pro Ala Gly Thr Phe
500 505 510
Gly Ala Arg Met Val Pro Lys Ala Leu Lys Ile Val Glu Ile Met Gly
515 520 525
Ile Lys Gln Ala Arg Ala Trp Gln Val Ala Ser Leu Asn Glu Phe Arg
530 535 540
Asp Phe Phe Gly Leu Lys Arg His Asp Thr Phe Lys Asp Ile Asn Ser
545 550 555 560
Asn Glu Glu Ile Ala Thr Leu Leu Glu Lys Leu Tyr Thr Asp Pro Asp
565 570 575
Met Val Glu Leu Tyr Pro Gly Leu Met Ile Glu Asp Ile Lys Pro Val
580 585 590
Arg Asn Thr Gly Ser Gly Ile Cys Pro Thr Tyr Ser Val Gly Arg Ala
595 600 605
Val Leu Ser Asp Ala Val Thr Leu Val Arg Ser Asp Arg Phe Asn Thr
610 615 620
Ile Asp Tyr Thr Val Ser Asn Leu Thr Ala Trp Gly Tyr Asn Glu Val
625 630 635 640
Gln Gln Asp Tyr Lys Thr Leu Gly Gly Ser Met Leu Tyr Lys Leu Ile
645 650 655
Gln Arg Gly Leu Pro Gly Trp Phe Pro Phe Asn Ser Ile Ala Val Met
660 665 670
Gln Pro Met Tyr Thr Lys Lys Ala Asn Glu Arg Ile Ala Arg Glu Ile
675 680 685
Gly Thr Phe Asn Gln Phe Thr Leu Asp Asp Pro Lys Ala Pro Pro Lys
690 695 700
Pro Val Val Val Ala Ser Ser Glu Gly Ile Lys Arg Val Leu Gly Ser
705 710 715 720
Pro Asp Lys Phe Val Val Pro Trp Leu Thr Pro Leu Asn Ala Leu Tyr
725 730 735
Thr Asp Thr Lys Lys Asp Ile Ser Trp Phe Met Leu Ala Gly Asp Gly
740 745 750
Ser Thr Asn Lys Gln Glu Lys Val Asn Phe Val Asn Ala Met Lys Lys
755 760 765
Val Pro Asn Leu His Asn Ala Val His Gln Phe Ile Glu Arg Val Gly
770 775 780
Arg Gln Leu Ile Glu Lys Glu Thr Phe Lys Leu Lys Glu Gly Leu Cys
785 790 795 800
Gln Met Asp Ile Ile Arg Asp Val Ala Ile Pro Leu Asn Ala Gln Leu
805 810 815
Leu Ala Asp Leu Phe Tyr Phe Asp Leu Arg His Glu Glu Asn Pro Gly
820 825 830
Gly Thr Leu Ser Ala Thr Asp Leu Tyr Arg His Leu Leu Asn Ile Arg
835 840 845
Ile Trp Gly Val Asn Asn Asn Asp Pro Gly Gln Ala Trp Asn Arg Arg
850 855 860
Arg Arg Ala Ala Glu Ser Ala Lys Val Ile Thr Asp Ser Thr Arg Lys
865 870 875 880
Leu Val Asp Glu Val Ser Arg Gly Arg Gly Leu Asn Leu Gly Phe Ile
885 890 895
Ser Ala Ile Asn Glu Val Ala Ser Arg Lys Thr His Ile Lys Lys Asp
900 905 910
Ser Leu Arg Ser Cys Gly Tyr Lys Leu Val Glu Glu Leu Leu Asn Gln
915 920 925
Gly Gly Ser Pro Glu Lys Val Thr Asp Asn Val Trp Leu Thr Ala Phe
930 935 940
Gly Gly Ile Gly Val Pro Val Thr Thr Phe Tyr Glu Val Met Glu Tyr
945 950 955 960
Phe Leu Arg Pro Glu Asn Lys Ser Ile Trp Gly Glu Val Gln Ala Leu
965 970 975
Ala Gln Lys Asn Asp Glu Ala Gly Leu His Ala Tyr Val Asn Glu Ala
980 985 990
Met Arg Leu Thr Ser Gly Gln Arg Asn Val Arg Ile Ala Thr Val Lys
995 10001005
Asp Glu Ile Asp Gly Gln Lys Val Glu Pro Gly Asn Ala Val Val
101010151020
Met Leu Leu Gly Ala Ala Gly Arg Asn Pro Lys Glu Val Pro Asn
102510301035
Ala Asp Lys Phe Asp Ala Lys Arg Ser Thr Asp His Ile Lys Pro
104010451050
Phe Ser Tyr Gly Gln His Glu Cys Val Gly Gln Asp Val Ala Arg
105510601065
Ala Phe Val Thr Gly Leu Val Lys Leu Val Ala Asp Leu Arg Gln
107010751080
Leu Arg Pro Ala Pro Gly Glu Met Gly Lys Val Lys Thr Ile Gln
108510901095
Val Gly Thr Glu Arg Ala Tyr Leu Asn Asp Ser Trp Ser Tyr Leu
110011051110
Gly Phe Asp Ala Ser Thr Trp Lys Val His Phe Asp Gly His Gly
111511201125
Gln Gly Thr Tyr Glu Gly Asp Pro Glu Pro Asn Lys Pro Ile Asp
113011351140
Met Gly Arg Tyr Tyr Tyr Ile Leu Gln Lys Arg Lys Glu Ser Leu
114511501155
Leu Lys Gly
1160
<210> 60
<211> 1283
<212> PRT
<213> 尖孢镰孢菌（Fusarium oxysporum）
<400> 60
Met Ala Glu His Lys Asn Gly Val Ala Thr Asn Gly Tyr Glu Lys Lys
1 5 1015
Ser Ser Pro Ala Ser Ser Ser Thr Lys Ser Glu Ala Lys Pro Leu Pro
202530
Asn Gly Asp Lys Lys Asp Gly Ile Val Lys Ser Phe Lys Gln Leu Arg
354045
Val Ala Ser Lys Arg Pro Leu Pro Lys Glu Met Gly Asp Gly Ser Tyr
505560
Arg Val Val Glu Asn Arg Pro Gly Leu Lys Gln Asp Ile Arg Arg Leu
65707580
Arg Gly Arg Asp Leu Lys Thr Leu Leu Glu Ile Val Lys Ala Lys Val
859095
Lys Gly Glu Thr Gln Gln Asp Asp Lys Thr Met Ile Met Glu Arg Thr
100 105 110
Ile Gln Leu Val Ala Asn Leu Ser Asp His Ser Lys Val Gln Glu Ser
115 120 125
Leu Thr Asn Ser Phe Ile Ser Gln Leu Trp Asn Ser Ile Asp His Pro
130 135 140
Pro Met Leu Tyr Met Gly Asp Lys Phe Arg Phe Arg Gln Pro Asp Gly
145 150 155 160
Ser Asn Asn Asn Pro Tyr Leu Pro Gln Leu Gly Ala Ala Arg Thr Pro
165 170 175
Tyr Ser Arg Thr Val Arg Pro Lys Gly Met Ser Leu Gly Ala Gln Pro
180 185 190
Asp Pro Glu Ala Ile Phe Glu Ser Val Phe Ala Arg Asp Ala Phe Arg
195 200 205
Lys Asn Pro Asn Asn Val Ser Ser Ile Leu Trp Tyr Trp Ala Thr Ile
210 215 220
Ile Ile His Asp Leu Phe Trp Thr Asn Leu Gln Asp Pro Asn Gln Asn
225 230 235 240
Asp Ser Ser Ser Tyr Leu Asp Leu Ala Pro Leu Tyr Gly Ser Thr Glu
245 250 255
Lys Asp Arg Asp Ser Ile Arg Thr Phe Lys Asp Gly Gln Leu Lys Pro
260 265 270
Asp Cys Phe Ala Asp Lys Arg Leu Ile Gly Asn Pro Pro Gly Val Pro
275 280 285
Ile Leu Leu Ile Met Phe Asn Arg Phe His Asn His Val Ala Thr Asn
290 295 300
Leu Ala Asp Ile Asn Glu Gly Gly Arg Phe Ser Lys Pro Ala Glu His
305 310 315 320
Leu Ser Pro Glu Ala Ala Asp Ala Ala Trp Lys Lys Arg Asp Thr Glu
325 330 335
Leu Phe Glu Thr Ala Arg Leu Val Thr Ser Gly Leu Tyr Ile Asn Ile
340 345 350
Thr Leu Ile Asp Tyr Val Arg Asn Ile Ile Asn Leu Asn Arg Val Asp
355 360 365
Thr Thr Trp Thr Leu Asp Pro Arg Gln Glu Met Gly Val Ser Val Gly
370 375 380
Thr Lys Asp Leu Ser Glu Ser Gly Thr Gly Asn Val Val Ser Ala Glu
385 390 395 400
Phe Asn Leu Cys Tyr Arg Trp His Ser Cys Leu Ser Glu Met Asp Asp
405 410 415
Lys Trp Val Gln Asp Phe Tyr Thr Glu Leu Leu Gly Glu Asn Tyr Gly
420 425 430
Pro Met Asn Leu Gln Thr Met Met Lys Ala Leu Lys Ala Phe Glu Ala
435 440 445
Ser Val Ala Asp Glu Pro Ser Glu Arg Thr Phe Gly Gly Phe Lys Arg
450 455 460
Gly Pro Asp Gly Lys Phe Asn Asp Asp Glu Leu Val Glu Ala Leu Ala
465 470 475 480
Thr Ala Ile Glu Gln Pro Gly Gly Ala Phe Gly Gly Arg Asn Val Pro
485 490 495
Arg Ile Met Lys Pro Ile Glu Met Leu Gly Ile Met Arg Gly Arg Lys
500 505 510
Trp Asn Leu Ala Gly Leu Asn Glu Phe Arg Lys His Phe Gly Leu Lys
515 520 525
Ala Tyr Glu Thr Phe Glu Asp Ile Asn Ser Asp Pro Ser Val Ala Asp
530 535 540
Ala Leu Arg Asn Leu Tyr Gln His Pro Asp Tyr Val Glu Leu Tyr Pro
545 550 555 560
Gly Ile Val Ala Glu Glu Ala Lys Thr Pro Met Val Pro Gly Val Gly
565 570 575
Ile Ala Pro Thr Tyr Thr Ile Ser Arg Val Val Leu Ser Asp Ala Val
580 585 590
Ala Leu Val Arg Gly Asp Arg Tyr Tyr Thr Thr Asp Tyr Asn Pro Arg
595 600 605
His Leu Thr Asn Trp Gly Tyr Lys Glu Val Asp Tyr Asp Leu Lys Val
610 615 620
Asn His Gly Cys Val Phe Tyr Lys Leu Phe Leu Arg Ala Phe Pro Gln
625 630 635 640
His Phe Lys Gly Asn Ser Val Tyr Ala His Tyr Pro Met Val Ile Pro
645 650 655
Ser Glu Asn Lys Lys Ile Leu Thr Asn Ile Lys Arg Ala Asp Arg Phe
660 665 670
Asp Phe Ser Arg Pro Glu Pro Thr Ala Thr Arg Ile Asn Ile Ile Gly
675 680 685
Tyr Asn Ala Ala Lys Tyr Ile Leu Glu Asp Gln Gln Lys Tyr Arg Val
690 695 700
Cys Trp Glu Glu Gly Leu Lys His Leu Met Gly Glu Ala Gly Gly Arg
705 710 715 720
Phe Met Leu Ser Gly Asp Thr Ala Leu His Ala Gln Gln Arg Lys Cys
725 730 735
Met Gly Lys Leu Leu Tyr Asn Asp Thr Trp Arg Asn Ala Val Lys Ser
740 745 750
Phe Tyr Ala Thr Thr Ala Glu Lys Leu Leu Ala Glu Lys Ser Tyr Arg
755 760 765
Leu Ala Gly Lys Met Gln Val Asp Val Val Arg Asp Val Gly Asn Val
770 775 780
Ala His Thr His Phe Val Ala Arg Met Phe Asn Leu Pro Leu Lys Thr
785 790 795 800
Ser Glu Asn Pro Lys Gly Val Phe Ser Glu Gln Glu Leu Tyr Met Ile
805 810 815
Leu Ala Val Ile Phe Val Cys Ile Phe Phe Asp Ile Asp Pro Ala Lys
820 825 830
Ser Phe Pro Leu Arg Gln Gly Ala Arg Glu Val Ala Gln Lys Leu Gly
835 840 845
Gly Ile Ile Glu Met Asn Val Lys Leu Ala Asn Ser Ile Gly Val Lys
850 855 860
Gly Leu Phe Thr Ser Lys Pro Asp Lys Asn Asp Asp Pro Leu Ala Arg
865 870 875 880
Tyr Gly Glu Asn Met Ala Lys Gly Leu Lys Lys Ala Gly Leu Ser Thr
885 890 895
Glu Asp Ile Val Trp Ser Gln Ile Leu Pro Thr Ala Gly Ala Met Val
900 905 910
Pro Asn Gln Ala Gln Val Phe Ala Gln Thr Leu Asp Trp Tyr Leu Ser
915 920 925
Pro Ala Gly Glu Lys Tyr Arg Pro Glu Leu Ala Arg Ile Ala Ala Leu
930 935 940
Glu Thr Gly Asp Glu Thr Asp Ala Leu Leu Leu Gly Tyr Ala Met Glu
945 950 955 960
Gly Ile Arg Met Ala Gly Thr Phe Gly Leu Tyr Arg Glu Ala Thr Gly
965 970 975
Pro Asp Thr Ile His Glu Asp Asp Gly Arg Ser Ile Pro Val Asn Ala
980 985 990
Gly Asp Arg Val Phe Val Ser Phe Val Gln Ala Ala Gln Asp Pro Lys
995 10001005
Ile Phe Pro Asn Pro Gly Val Val Asp Pro Lys Arg Pro Leu Asp
101010151020
Lys Tyr Ile His Tyr Gly Val Gly Pro His Ala Cys Leu Gly Arg
102510301035
Asp Ile Ser Gln Val Ala Leu Thr Glu Leu Phe Arg Ala Val Phe
104010451050
Arg Lys Lys Gly Val Arg Arg Val Pro Gly Ala Gln Gly Glu Leu
105510601065
Lys Lys Val Pro Arg Pro Gly Gly Phe Phe Val Tyr Met Thr Glu
107010751080
Asp Trp Gly Ser Ile Trp Pro Phe Pro Thr Ser Met Lys Ile Thr
108510901095
Trp Asp Asp Gly Cys Gly Thr Asp Leu Phe Thr Leu Leu Phe Trp
110011051110
Cys Arg Ser Val Pro Trp Trp Asp Leu Leu Pro Glu Thr Thr Gln
111511201125
Ala Ala Pro Phe Ile Ile Ser Ser Phe Gln Ser Gln Gln Ile Tyr
113011351140
Ser Pro Ala Glu Ile Ala Ala Val Leu Ala Leu Met Ala Phe Glu
114511501155
Ser Gly Asp Phe Gln Tyr Lys Arg Asn His Tyr Pro Gly Arg Pro
116011651170
Gly Gln Gly Thr Ala Asn Met Gln Met Pro Asn Tyr Asn Leu Leu
117511801185
Tyr Ala Lys Ser Ile Pro Glu Leu Ala Lys Gly Trp Gln Gly Ile
119011951200
Glu Ser Val Glu Gly Leu Ser Asp Gln Glu Leu Gly Asp Leu Leu
120512101215
Asp Asp Val Thr Val Asp Lys Tyr Asn Phe Gly Ser Gly Pro Trp
122012251230
Phe Leu Lys Thr Gln Cys Lys Glu Asp Val Arg Gln Ala Phe Lys
123512401245
Thr Asp Val Asp Thr Gly Phe Gln Lys Tyr Ile Glu Glu Cys Val
125012551260
Gly Thr Asp Leu Gln Pro Arg Leu Glu Tyr Phe Gln Arg Ala Lys
126512701275
Thr Ala Phe Gly Leu
1280
<210> 61
<211> 1079
<212> PRT
<213> 烟曲霉（Aspergillus fumigatus）
<400> 61
Met Ser Glu Lys Gln Thr Gly Ser Ala Asn Gly Gly Leu Gly Lys Thr
1 5 1015
Leu Ala Gln Leu Glu Gln Val Val Ser Ala Ser Leu Arg Pro Leu Pro
202530
Ser Gln Thr Gly Asp Gly Thr Tyr Val Thr Glu Gln Val Lys Thr Gly
354045
Ile Leu Lys Asp Leu Ser His Val Asp Leu Gly Asp Leu Lys Thr Leu
505560
Val Asp Val Ser Lys Ser Ala Leu Thr Gly Glu Ala Leu Asp Asp Arg
65707580
Lys Tyr Ile Met Glu Arg Val Ile Gln Leu Ser Ala Gly Leu Pro Ser
859095
Thr Ser Gln Ile Gly Lys Glu Leu Thr Asn Thr Phe Leu Thr Thr Leu
100 105 110
Trp Asn Asp Leu Glu His Pro Pro Ile Ser Tyr Leu Gly Arg Asp Ala
115 120 125
Met Tyr Arg Arg Ala Asp Gly Ser Gly Asn Asn Val Leu Trp Pro His
130 135 140
Ile Gly Ala Ala Gly Thr Pro Tyr Ala Arg Ser Val Gln Pro Lys Thr
145 150 155 160
Val Gln Ser Pro Asn Leu Pro Asp Pro Glu Thr Leu Phe Asp Cys Leu
165 170 175
Leu Ala Arg Lys Glu Tyr Lys Glu His Pro Asn Lys Ile Ser Ser Val
180 185 190
Leu Phe Tyr Ile Ala Ser Ile Ile Ile His Asp Leu Phe Glu Thr Asp
195 200 205
Arg Lys Asp Pro Ala Ile Ser Leu Thr Ser Ser Tyr Leu Asp Leu Ser
210 215 220
Pro Leu Tyr Gly Asn Asn Gln Gln Glu Gln Asp Leu Ile Arg Thr Phe
225 230 235 240
Lys Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Thr Lys Arg Val Leu
245 250 255
Gly Phe Pro Pro Gly Val Gly Val Val Leu Ile Met Phe Asn Arg Phe
260 265 270
His Asn Tyr Val Val Glu Lys Leu Ala Met Ile Asn Glu Gly Gly Arg
275 280 285
Phe Thr Lys Pro Gln Glu Ser Asp Thr Ala Ala Tyr Ala Lys Tyr Asp
290 295 300
Asn Asp Leu Phe Gln Thr Gly Arg Leu Val Thr Cys Gly Leu Tyr Val
305 310 315 320
Asn Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg
325 330 335
Thr Asp Ser Ile Trp Ser Leu Asp Pro Arg Ser Glu Met Lys Asp Gly
340 345 350
Leu Leu Gly Arg Ala Ala Ala Gln Ala Thr Gly Asn Gln Val Ala Ala
355 360 365
Glu Phe Asn Leu Val Tyr Arg Trp His Ser Cys Ile Ser Gln Arg Asp
370 375 380
Gln Lys Trp Thr Glu Asp Met Tyr Gln Glu Leu Phe Pro Gly Gln Asp
385 390 395 400
Pro Ser Lys Ile Ser Leu Gln Asp Phe Leu Arg Gly Leu Gly Arg Trp
405 410 415
Glu Ala Lys Leu Pro Gly Glu Pro Arg Glu Arg Pro Phe Ala Gly Leu
420 425 430
Gln Arg Lys Ala Asp Gly Ser Tyr Asp Asp Asn Asp Leu Val Lys Ile
435 440 445
Phe Glu Glu Ser Val Glu Asp Cys Ala Gly Ala Phe Gly Ala Leu His
450 455 460
Val Pro Thr Val Phe Arg Ser Ile Glu Ala Leu Gly Ile Gln Gln Ala
465 470 475 480
Arg Ser Trp Asn Leu Ala Thr Leu Asn Glu Phe Arg Lys Tyr Phe Asn
485 490 495
Leu Ala Pro Tyr Lys Thr Phe Glu Glu Ile Asn Ser Asp Pro Tyr Val
500 505 510
Ala Asp Gln Leu Lys Arg Leu Tyr Asp His Pro Asp Arg Val Glu Ile
515 520 525
Tyr Pro Gly Ile Ile Val Glu Asp Ala Lys Glu Ser Met Ala Pro Gly
530 535 540
Ser Gly Leu Cys Thr Asn Phe Thr Ile Ser Arg Ala Ile Leu Ser Asp
545 550 555 560
Ala Val Ala Leu Val Arg Gly Asp Arg Phe His Thr Val Asp Phe Thr
565 570 575
Pro Lys His Leu Thr Asn Trp Ala Tyr Asn Glu Ile Gln Pro Gln Asp
580 585 590
Ser Val Asp Gln Thr His Val Phe Tyr Lys Leu Val Leu Arg Ala Phe
595 600 605
Pro Asn His Phe Arg Gly Asp Ser Ile Tyr Ala His Phe Pro Leu Val
610 615 620
Val Pro Ser Glu Asn Lys Lys Ile Leu Thr Lys Leu Gly Thr Ala Asp
625 630 635 640
Lys Tyr Ser Trp Asp Arg Pro Asn Tyr Thr Pro Pro Pro Gln Phe Ile
645 650 655
Asn Ser His Ser Ala Cys Met Ser Ile Leu Ser Asp Gln Glu Thr Phe
660 665 670
Lys Val Thr Trp Gly Ser Lys Ile Glu Phe Leu Met Arg His Asn Asn
675 680 685
Gln Pro Tyr Gly Arg Asp Phe Met Leu Ser Gly Asp Arg Thr Pro Asn
690 695 700
Ala Met Ser Arg Gln Met Met Gly Lys Ala Leu Tyr Arg Asp Lys Trp
705 710 715 720
Glu Thr Glu Val Lys Arg Phe Tyr Glu Asn Ile Thr Leu Lys Leu Leu
725 730 735
His Arg Tyr Ser Tyr Lys Leu Ala Gly Val Asn Gln Val Asp Val Val
740 745 750
Arg Asp Ile Ala Asn Leu Ala Gln Val His Phe Cys Ala Ser Val Phe
755 760 765
Ser Leu Pro Leu Lys Thr Glu Ser Asn Pro Arg Gly Ile Phe Thr Glu
770 775 780
Ser Glu Leu Tyr Gln Ile Met Ala Val Val Phe Thr Ser Ile Phe Tyr
785 790 795 800
Asp Ala Asp Ile Gly Lys Ser Phe Glu Leu Asn Gln Ala Ala Arg Ala
805 810 815
Val Thr Gln Gln Leu Gly Gln Leu Thr Leu Ala Asn Val Glu Leu Ile
820 825 830
Ala Lys Thr Gly Phe Ile Ala Asn Leu Val Asn Ser Leu His Arg His
835 840 845
Asp Val Leu Ser Glu Tyr Gly Val His Met Ile Gln Arg Leu Leu Asp
850 855 860
Ser Gly Met Pro Ala Pro Glu Ile Val Trp Thr His Val Leu Pro Thr
865 870 875 880
Ala Gly Gly Met Val Ala Asn Gln Ala Gln Leu Phe Ser Gln Ser Leu
885 890 895
Asp Tyr Tyr Leu Ser Glu Glu Gly Ser Val His Leu Pro Glu Ile Asn
900 905 910
Arg Leu Ala Lys Glu Asp Thr Thr Glu Ala Asp Asp Leu Leu Leu Arg
915 920 925
Tyr Phe Met Glu Gly Ala Arg Ile Arg Ser Ser Val Ala Leu Pro Arg
930 935 940
Val Val Ala Gln Pro Thr Val Val Glu Asp Asn Gly Gln Lys Ile Thr
945 950 955 960
Leu Lys Gln Gly Gln His Ile Ile Cys Asn Leu Val Ser Ala Ser Met
965 970 975
Asp Pro Val Thr Phe Pro Glu Pro Asp Lys Val Lys Leu Asp Arg Asp
980 985 990
Met Asn Leu Tyr Ala His Phe Gly Phe Gly Pro His Gln Cys Leu Gly
995 10001005
Leu Gly Leu Cys Lys Thr Ala Leu Thr Thr Met Leu Lys Val Ile
101010151020
Gly Arg Leu Asp Asn Leu Arg Arg Ala Pro Gly Gly Gln Gly Lys
102510301035
Leu Lys Lys Leu Ser Gly Pro Gly Gly Ile Ala Met Tyr Met Thr
104010451050
Pro Asp Gln Thr Ala Phe Phe Pro Phe Pro Thr Thr Met Lys Ile
105510601065
Gln Trp Asp Gly Asp Leu Pro Glu Val Lys Glu
10701075
<210> 62
<211> 1071
<212> PRT
<213> 土曲霉（Aspergillus terreus）
<400> 62
Met Gln Gly Ile Gly Lys Ala Ile Ser Gln Leu Glu Lys Val Ala Thr
1 5 1015
Ala Ser Leu Arg Pro Leu Pro Thr Glu Thr Gly Asp Gly Ser Tyr Val
202530
Ala Glu Ser Thr Ala Thr Gly Leu Val Gln Asp Leu Pro His Val Asp
354045
Leu Gly Asp Leu Lys Thr Leu Leu Asp Val Thr Lys Asn Ala Ala Thr
505560
Gly Glu Pro Ile Asp Asp Lys Gly Tyr Val Met Glu Arg Leu Ile Gln
65707580
Leu Ala Ser Gly Leu Pro Ser Thr Ser Arg Asn Ala Lys Gln Leu Thr
859095
Ser Ala Phe Leu Asn Gln Leu Trp Asn Asp Leu Asp His Pro Pro Val
100 105 110
Ser Thr Val Gly Gly Glu Tyr Ser His Arg Ser Ala Asp Gly Ser Gly
115 120 125
Asn Asn Ile Leu Trp Pro Gly Ile Gly Ala Ala Gly Ser His Tyr Ala
130 135 140
Arg Ser Val Gln Pro Lys Thr Met Gln Ser Pro Ser Leu Pro Asp Pro
145 150 155 160
Glu Ala Leu Phe Asp Ser Leu Leu Ala Arg Lys Asp Phe Lys Glu His
165 170 175
Pro Asn Lys Ile Ser Ser Val Leu Phe Tyr Ile Ala Ser Ile Ile Ile
180 185 190
His Asp Leu Phe Gln Thr Asp His Arg Asp Ser Ser Ile Asn Arg Thr
195 200 205
Ser Ser Tyr Leu Asp Leu Ser Pro Leu Tyr Gly Asn Asn Gln Asp Glu
210 215 220
Gln Tyr Leu Met Arg Thr Phe Lys Asp Gly Lys Leu Lys Pro Asp Cys
225 230 235 240
Phe Ser Ser Lys Arg Ile Leu Gly Phe Pro Pro Gly Val Gly Val Leu
245 250 255
Leu Ile Met Phe Asn Arg Phe His Asn Tyr Val Val Glu Gln Leu Ala
260 265 270
Ala Val Asn Glu Gly Gly Arg Phe Thr Lys Pro Ser Glu Ser Asn Asp
275 280 285
Lys Glu Tyr Ala Lys Tyr Asp Asn Asn Leu Phe Gln Thr Gly Arg Leu
290 295 300
Val Thr Cys Gly Leu Tyr Ile Asn Ile Ile Leu Lys Asp Tyr Val Arg
305 310 315 320
Thr Ile Leu Asn Ile Asn Arg Thr Asn Ser Thr Trp Ser Leu Asp Pro
325 330 335
Arg Met Asp Met Lys Asp Gly Leu Leu Gly Asp Ala Ala Pro Leu Ala
340 345 350
Thr Gly Asn Gln Val Ser Ala Glu Phe Asn Leu Ile Tyr Arg Trp His
355 360 365
Ser Cys Ile Ser Gln Arg Asp Glu Lys Trp Thr Thr Asp Leu Tyr Asn
370 375 380
Asp Ile Phe Ser Asp Lys Gly Gln Glu Asp Ile Pro Leu Asn Glu Phe
385 390 395 400
Met Met Gly Val Gly Lys Trp Glu Ala Gly Leu Pro Gln Gln Pro Ala
405 410 415
Glu Arg Pro Phe Ala Gly Leu Lys Arg Lys Pro Asn Gly Leu Phe Asp
420 425 430
Asp Asp Asp Leu Val Thr Ile Phe Lys Glu Ser Val Glu Asp Cys Ala
435 440 445
Gly Ala Phe Gly Ala Ser His Val Pro Thr Ile Phe Lys Ser Ile Glu
450 455 460
Ser Leu Gly Ile Lys Gln Ala Arg Ala Trp Asn Leu Ala Thr Leu Asn
465 470 475 480
Glu Leu Arg Gln Tyr Phe Gly Leu Thr Pro His Lys Thr Phe Glu Asp
485 490 495
Ile Asn Ser Asp Pro Tyr Ile Ser Glu Gln Leu Arg Arg Leu Tyr Asp
500 505 510
His Pro Asp Gln Val Glu Ile Tyr Pro Gly Val Ile Val Glu Glu Thr
515 520 525
Lys Glu Ser Met Leu Pro Gly Ser Gly Leu Cys Thr Asn Phe Thr Ile
530 535 540
Ser Arg Ala Ile Leu Ser Asp Ala Val Ala Leu Val Arg Gly Asp Arg
545 550 555 560
Phe Tyr Thr Val Asp Tyr Thr Pro Lys Gln Leu Thr Asn Trp Ala Phe
565 570 575
Thr Glu Ile Gln Pro Lys Asp Ser Val Asp Gln Gly His Met Phe His
580 585 590
Lys Leu Val Tyr Arg Ala Phe Pro Asn Tyr Phe Lys Gly Asn Ser Val
595 600 605
Tyr Ala His Phe Pro Met Val Val Pro Ser Glu Asn Gln Lys Ile Leu
610 615 620
Thr Ala Leu Gly Ser Ala Glu Lys Tyr Ser Trp Asp Lys Pro Gly Phe
625 630 635 640
Ile His Pro Pro Gln Phe Ile Asn Ser His Ser Thr Cys Val Ser Ile
645 650 655
Leu Ala Asp Gln Glu Thr Phe Lys Val Ser Trp Gly Asp Lys Ile Glu
660 665 670
Phe Leu Met Ser Asn His Asp Lys Ile Tyr Gly Lys Asp Phe Met Leu
675 680 685
Ser Gly Asp Arg Leu Pro Asn Ala Glu Ser Arg Lys Met Met Gly Ala
690 695 700
Ala Leu Tyr Thr Asp Gln Trp Glu Glu Glu Val Lys Lys Phe Tyr Glu
705 710 715 720
Lys Ile Thr Leu Lys Leu Leu Lys Lys His Ser Tyr Lys Ile Ala Gly
725 730 735
Val Asn Gln Val Asp Ile Val Arg Asp Val Ala Asn Leu Ala Gln Val
740 745 750
Asn Phe Cys Ala Asn Val Phe Ser Leu Pro Leu Lys Thr Glu Ala Ser
755 760 765
Pro Arg Gly Ile Phe Thr Glu Ser Glu Leu Tyr Met Ile Met Ala Ala
770 775 780
Val Phe Ala Ala Ile Phe Tyr Asp Ala Asp Pro Ala Asn Ser Phe Ala
785 790 795 800
Leu Asn Gln Ala Ala Arg Glu Val Thr Gln Gln Leu Gly Gln Val Thr
805 810 815
Met Ala Asn Val Glu Leu Ile His Lys Thr Gly Phe Ile Ser Asn Leu
820 825 830
Val Asn Gly Leu Gln Arg His Asp Val Leu Ser Asn Tyr Gly Ile His
835 840 845
Met Ile Gln Arg Leu Leu Ala Ser Gly Leu Pro Ala Ser Glu Ile Val
850 855 860
Trp Thr His Leu Leu Pro Thr Ala Gly Gly Met Val Ala Asn Gln Gly
865 870 875 880
Gln Leu Phe Ser Gln Cys Leu Asp Tyr Tyr Leu Ser Glu Glu Gly Ser
885 890 895
Val His Leu Pro Glu Ile Asn Arg Leu Ala Lys Glu Asn Thr Pro Glu
900 905 910
Ala Asp Glu Leu Leu Leu Arg Tyr Phe Met Glu Gly Ala Arg Leu Arg
915 920 925
Ser Ser Val Gly Leu Pro Arg Val Val Ala Lys Pro Thr Val Ile Asp
930 935 940
Asp Asn Gly Thr Lys Leu Thr Leu Lys Glu Gly Gln His Ile Leu Cys
945 950 955 960
Asn Leu Val Ala Ala Ser His Asp Pro Val Ser Phe Pro Glu Pro Glu
965 970 975
Lys Val Arg Leu Asp Arg Asp Met Asp Leu Tyr Val His Phe Gly Ser
980 985 990
Gly Pro His Lys Cys Leu Gly Phe Gly Leu Cys Lys Leu Gly Leu Thr
995 10001005
Thr Met Leu Lys Val Val Gly Gly Leu Asp Asn Leu Arg Arg Ala
101010151020
Pro Gly Pro Gln Gly Gln Leu Lys Arg Leu Ala Gly Pro Gly Gly
102510301035
Ile Ser Lys Tyr Met Thr Ala Asp Gln Ser Gly Phe Phe Pro Phe
104010451050
Pro Thr Thr Met Lys Ile Gln Trp Asp Gly Asp Leu Pro Glu Pro
105510601065
Ala Ser Asp
1070
<210> 63
<211> 1080
<212> PRT
<213> 白曲霉（Aspergillus kawachii）
<400> 63
Met Ser Gly Ser Asn Asn His Ser Ile Val Asn Gly Ile Gly Ser Thr
1 5 1015
Ile Ser Gln Val Glu Lys Ala Ile Ser Ala Ser Leu Arg Pro Leu Pro
202530
Thr Ala Thr Gly Asn Gly Thr Tyr Val Thr Glu Pro Ala Gln Thr Gly
354045
Ile Val Lys Asp Leu Ser His Val Asp Leu Thr Asp Phe Lys Ala Leu
505560
Leu Glu Val Val Lys Asp Ala Val Thr Gly Gln Pro Val Asp Asp Arg
65707580
His Tyr Ile Met Glu Arg Val Ile Gln Leu Ala Ala Gly Leu Pro Ser
859095
Thr Ser Lys Ser Gly Lys Asp Leu Thr Asn Thr Phe Leu Lys Gln Leu
100 105 110
Trp Asn Asp Leu Glu His Pro Pro Ile Ser Tyr Leu Gly Arg Asn Thr
115 120 125
Ser Tyr Arg Lys Ala Asp Gly Ser Gly Asn Asn Phe Leu Trp Pro His
130 135 140
Ile Gly Ala Ala Gly Ser His Tyr Ala Arg Ser Val Arg Pro Thr Thr
145 150 155 160
Val Gln Ser Pro Ser Leu Pro Asp Pro Ser Thr Leu Phe Glu Ser Leu
165 170 175
Leu Glu Arg Lys Glu Tyr Lys Glu His Pro Asn Lys Ile Ser Ser Val
180 185 190
Leu Phe Tyr Leu Ala Ser Ile Ile Ile His Asp Leu Phe Gln Thr Asp
195 200 205
Arg Asn Asp Tyr Thr Leu Asn Lys Thr Ser Ser Tyr Leu Asp Leu Ser
210 215 220
Pro Leu Tyr Gly Asn Asn Gln Asp Glu Gln Asn Leu Val Arg Ser Phe
225 230 235 240
Lys Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Ser Lys Arg Val Leu
245 250 255
Gly Phe Pro Pro Gly Val Gly Val Leu Leu Leu Met Phe Asn Arg Phe
260 265 270
His Asn Tyr Val Val Glu Asn Leu Ala Thr Ile Asn Glu Gly Gly Arg
275 280 285
Phe Thr Lys Pro Asp Glu Ser Asp Val Asp Ala Ser Thr Arg Tyr Asp
290 295 300
Asn Asp Leu Phe Gln Thr Gly Arg Leu Val Thr Cys Gly Leu Tyr Ile
305 310 315 320
Asn Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg
325 330 335
Thr Asp Ser Leu Trp Ser Leu Asp Pro Arg Ala Asp Ile Gln Asp Ser
340 345 350
Leu Leu Gly Ser Ala Pro Ala Glu Ala Thr Gly Asn Gln Val Ser Ala
355 360 365
Glu Phe Asn Leu Val Tyr Arg Trp His Ala Cys Val Ser Gln Arg Asp
370 375 380
Glu Lys Trp Thr Gln Asp Leu Tyr Lys Asp Leu Phe Pro Gly Lys Asp
385 390 395 400
Pro Asn Asn Val Ser Leu Pro Glu Phe Leu Arg Gly Val Ala Lys Trp
405 410 415
Glu Ala Ser Leu Pro Glu Gln Pro Pro Asp Arg Pro Phe Ala Gly Leu
420 425 430
Gln Arg Asn Ala Asp Gly Ala Phe Asp Asp Asp Asp Leu Ala Asn Met
435 440 445
Phe Ala Asp Gly Val Glu Asp Cys Ala Gly Ala Phe Gly Ala Gly Asn
450 455 460
Ile Pro Ser Val Phe Arg Asn Ile Glu Ala Leu Gly Ile Leu Gln Ala
465 470 475 480
Arg Ser Trp Asn Leu Ala Thr Leu Asn Glu Phe Arg Lys Phe Phe Asp
485 490 495
Leu Ala Pro Tyr Lys Thr Phe Glu Glu Ile Asn Pro Asp Pro Tyr Ile
500 505 510
Ala Ala Gln Leu Lys Asn Leu Tyr Asp Glu Pro Asp Leu Val Glu Met
515 520 525
Tyr Pro Gly Val Ile Val Glu Ala Thr Lys Asp Ala Val Val Pro Gly
530 535 540
Ser Gly Leu Cys Thr Asn Phe Thr Ile Ser Arg Ala Ile Leu Ser Asp
545 550 555 560
Ala Val Ser Leu Val Arg Gly Asp Arg Phe Tyr Thr Val Asp Tyr Thr
565 570 575
Pro Lys His Leu Thr Asn Trp Ala Tyr His Glu Ile Gln Pro Gln Asp
580 585 590
Ser Val Asp Gln Gly Gln Val Phe Tyr Lys Leu Val Leu Arg Ala Phe
595 600 605
Pro Asn His Phe Lys Gly Asp Ser Ile Tyr Ala His Phe Pro Leu Val
610 615 620
Ile Pro Ser Glu Asn Lys Lys Ile Leu Glu Lys Leu Ser Val Ala Gln
625 630 635 640
Asp Tyr Ser Trp Gly Arg Pro Ser Tyr Thr Pro Thr Pro Gln Phe Ile
645 650 655
Ser Ser Asn Ala Ala Cys Ile Ser Val Leu Asn Asp Gln Glu Ala Phe
660 665 670
Lys Val Thr Trp Gly Ser Lys Ile Glu Phe Leu Met Arg His Asn Asn
675 680 685
His Pro Tyr Gly Arg Asp Phe Met Leu Ser Gly Asp Lys Pro Pro Asn
690 695 700
Ala Ala Ser Arg Arg Met Met Gly Ser Ala Leu Tyr Arg Asp Lys Trp
705 710 715 720
Glu Ser Glu Val Lys Arg Phe Tyr Glu Asp Ile Thr Ile Lys Leu Leu
725 730 735
Arg Gln His Ser Tyr Lys Leu Gly Gly Thr Asn Gln Val Asp Ile Val
740 745 750
Arg Asp Val Ala Asn Tyr Ala Gln Val His Phe Cys Ala Asn Val Phe
755 760 765
Ser Leu Pro Leu Lys Thr Glu Ser Asn Pro Arg Gly Ile Phe Thr Glu
770 775 780
Ala Glu Leu Tyr Glu Ile Leu Ala Leu Val Phe Ala Ser Ile Phe Tyr
785 790 795 800
Asp Ala Asp Val Gly Thr Ser Phe Gln Leu Asn His Thr Ala Arg Asp
805 810 815
Val Thr Gln Gln Leu Gly Asp Leu Thr Met Ala Asn Val Asp Phe Val
820 825 830
Asn Lys Ala Gly Phe Ile Ala Asn Ile Val Ser Ser Leu His Arg His
835 840 845
Asp Val Leu Ser Glu Tyr Gly Glu His Met Ile Gln Arg Leu Leu His
850 855 860
Asn Asn Ile Pro Pro Ala Asp Ile Val Trp Thr His Leu Leu Pro Thr
865 870 875 880
Ala Gly Gly Met Val Ala Asn Gln Ala Gln Leu Phe Ser Gln Cys Leu
885 890 895
Asp Tyr Tyr Leu Ser Asp Glu Gly Ser Val His Leu Pro Asp Ile Lys
900 905 910
Arg Leu Ala Gln Glu Asn Thr Ser Glu Ala Asp Ser Leu Leu Leu Arg
915 920 925
Tyr Phe Met Glu Gly Ala Arg Leu Arg Ser Ser Val Gly Leu Pro Arg
930 935 940
Leu Val Ala Lys Pro Thr Val Val Asp Asp Gly Gly Ser Lys Tyr Thr
945 950 955 960
Leu Lys Pro Gly Gln Ser Val Leu Cys Asn Leu Val Ser Ala Ser Met
965 970 975
Asp Pro Arg Ser Phe Pro Glu Pro Glu Lys Val Lys Leu Asp Arg Asp
980 985 990
Met Ser Leu Tyr Ala His Phe Gly Phe Gly Pro His Gln Cys Leu Gly
995 10001005
Met Gly Ile Cys Lys Leu Ala Leu Thr Thr Met Leu Arg Val Val
101010151020
Gly Arg Leu Asp Asn Leu Arg Arg Ala Pro Gly Ser Gln Gly Gln
102510301035
Leu Lys Arg Ile Ala Gly Pro Gly Gly Ile Ser Met Tyr Met Thr
104010451050
Ala Asp Gln Ser Ser Tyr Trp Pro Phe Pro Ser Thr Met Lys Ile
105510601065
Gln Trp Asp Gly Asp Leu Pro Ser Leu Ala Thr Asn
107010751080
<210> 64
<211> 1080
<212> PRT
<213> 棒曲霉（Aspergillus clavatus）
<400> 64
Met Ser Gly Lys Arg Glu Glu Ser Thr Asn Gly Gly Ile Gly Lys Thr
1 5 1015
Ile Ala Gln Ile Glu Lys Val Val Thr Ala Ser Leu Arg Pro Leu Pro
202530
Ser Lys Thr Gly Asp Gly Thr Tyr Val Thr Glu Ala Val Lys Thr Gly
354045
Leu Leu Lys Asp Leu Ser His Val Asp Leu Gly Asp Leu Lys Thr Leu
505560
Leu Glu Val Ser Lys Ser Ala Leu Thr Gly Glu Ala Val Asp Asp Lys
65707580
Lys Tyr Ile Met Glu Arg Val Val Gln Leu Ala Ala Gly Leu Pro Ser
859095
Thr Ser Gln Ile Gly Lys Asp Met Thr Asn Thr Phe Leu Thr Thr Leu
100 105 110
Trp Asn Asp Leu Glu His Pro Pro Ile Ser Tyr Leu Gly Arg Asp Ala
115 120 125
Met Tyr Arg Lys Ala Asp Gly Ser Gly Asn Asn Ile Leu Trp Pro His
130 135 140
Ile Gly Ala Ala Gly Thr Pro Tyr Ala Arg Ser Val Arg Pro Lys Thr
145 150 155 160
Met Gln Ser Pro Asn Leu Pro Asp Pro Glu Thr Leu Phe Asp Cys Leu
165 170 175
Leu Ala Arg Lys Glu Tyr Lys Glu His Pro Asn Lys Ile Ser Ser Val
180 185 190
Leu Phe Tyr Ile Ala Ser Ile Ile Ile His Asp Ile Phe Gln Thr Asp
195 200 205
Arg Lys Asp Pro Thr Val Ser Leu Thr Ser Ser Tyr Leu Asp Leu Ser
210 215 220
Pro Leu Tyr Gly Asn Asn Gln Asp Glu Gln Asn Leu Val Arg Thr Phe
225 230 235 240
Lys Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Thr Lys Arg Val Leu
245 250 255
Gly Phe Pro Pro Gly Val Gly Val Leu Leu Ile Met Phe Asn Arg Phe
260 265 270
His Asn His Val Val Glu Asn Leu Ala Leu Ile Asn Glu Gly Gly Arg
275 280 285
Phe Thr Lys Pro Gln Glu Ser Asp Ala Gln Ala Tyr Ala Lys Tyr Asp
290 295 300
Asn Asp Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys Gly Leu Tyr Val
305 310 315 320
Asn Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg
325 330 335
Thr Asp Ser Ile Trp Ser Leu Asp Pro Arg Ala Asp Met Lys Asp Gly
340 345 350
Leu Leu Gly Glu Ala Ala Ala Gln Ala Thr Gly Asn Gln Val Ser Ala
355 360 365
Glu Phe Asn Leu Val Tyr Arg Trp His Ser Cys Ile Ser Lys Arg Asp
370 375 380
Gln Lys Trp Thr Glu Asp Met Tyr Gln Glu Val Phe Pro Gly Gln Asp
385 390 395 400
Pro Ser Lys Leu Pro Leu Gln Asp Phe Met Arg Gly Leu Gly Arg Trp
405 410 415
Glu Ala Lys Leu Pro Gly Glu Pro Gln Glu Arg Pro Phe Ala Gly Leu
420 425 430
Gln Arg Lys Ala Asp Gly Ser Tyr Asp Asp Asp Asp Leu Val Lys Ile
435 440 445
Phe Gly Asp Ser Val Glu Asp Cys Ala Gly Ala Phe Gly Val Leu His
450 455 460
Val Pro Thr Val Phe Arg Ser Ile Glu Ala Leu Gly Ile Gln Gln Ala
465 470 475 480
Arg Ser Trp Asn Leu Ala Thr Leu Asn Glu Phe Arg Asn Tyr Phe Asn
485 490 495
Leu Ala Pro Tyr Lys Thr Phe Glu Glu Ile Asn Pro Asp Pro Tyr Val
500 505 510
Ala Asp Gln Leu Arg Arg Leu Tyr Asp His Pro Asp Arg Val Glu Ile
515 520 525
Tyr Pro Gly Ile Ile Val Glu Asp Thr Lys Glu Ser Met Ala Pro Gly
530 535 540
Ser Gly Leu Cys Thr Asn Phe Thr Ile Ser Arg Ala Ile Leu Ser Asp
545 550 555 560
Ala Val Ala Leu Val Arg Gly Asp Arg Phe His Thr Val Asp Phe Thr
565 570 575
Pro Lys His Leu Thr Asn Trp Ala Tyr Asn Glu Ile Gln Pro Gln Glu
580 585 590
Ser Val Asp Gln Thr His Val Leu Tyr Lys Leu Val Phe Arg Ala Phe
595 600 605
Pro Asn His Phe Lys Gly Asp Ser Ile Tyr Ala His Phe Pro Leu Val
610 615 620
Ile Pro Ser Glu Asn Lys Lys Ile Leu Thr Lys Leu Gly Thr Ala Asp
625 630 635 640
Lys Tyr Ser Trp Asp Arg Pro Ser Phe Thr His Pro Pro Gln Phe Ile
645 650 655
Asn Ser His Ser Ala Cys Met Ser Ile Leu Ala Asp Gln Glu Thr Phe
660 665 670
Lys Val Ser Trp Gly Lys Lys Ile Glu Phe Leu Met Arg His Asn Asp
675 680 685
Gln Pro His Gly Arg Asp Phe Met Leu Ser Gly Asp Lys Pro Pro Asn
690 695 700
Ala Glu Ser Arg Gln Met Met Gly Lys Ala Leu Tyr Arg Asn Lys Trp
705 710 715 720
Glu Thr Glu Val Lys Asp Phe Tyr Glu Glu Ile Thr Leu Lys Leu Leu
725 730 735
His Arg Asn Ser Tyr Lys Leu Ala Gly Thr Asn Gln Val Asp Ile Val
740 745 750
Arg Asp Val Ala Asn Leu Ala Gln Val Tyr Phe Cys Ala Ser Val Phe
755 760 765
Ser Leu Pro Leu Lys Thr Glu Ser Asn Pro Arg Gly Val Phe Thr Glu
770 775 780
Ser Glu Leu Tyr Gln Ile Met Ala Val Val Phe Thr Ser Ile Phe Tyr
785 790 795 800
Asp Ala Asp Val Cys Lys Ser Phe Glu Leu Asn Gln Gly Ala Arg Ala
805 810 815
Val Thr Gln Gln Leu Gly Gln Leu Thr Met Ala Asn Val Glu Leu Ile
820 825 830
Glu Lys Thr Gly Phe Ile Ala Asn Leu Val Asn Ser Leu His Arg His
835 840 845
Asp Ala Leu Thr Glu Tyr Gly Val His Met Ile Gln Arg Leu Leu Asp
850 855 860
Thr Gly Thr Pro Ala Ala Glu Ile Val Trp Thr His Val Leu Pro Thr
865 870 875 880
Ala Gly Gly Met Val Ala Asn Gln Ala Gln Leu Phe Ser Gln Cys Leu
885 890 895
Asp Tyr Tyr Leu Ser Asp Glu Gly Ser Ile His Leu Pro Glu Ile Asn
900 905 910
Arg Leu Ala Lys Gln Asp Thr Pro Glu Ala Asp Asp Leu Leu Leu Arg
915 920 925
Tyr Phe Met Glu Gly Ala Arg Leu Arg Ser Ser Val Ala Leu Pro Arg
930 935 940
Val Val Ala Gln Pro Thr Val Val Glu Asp Asn Gly Gln Lys Val Ile
945 950 955 960
Leu Lys Glu Gly Gln His Ile Leu Cys Asn Leu Val Ser Ala Ser Met
965 970 975
Asp Pro Ala Ser Phe Pro Glu Pro Asp Lys Val Lys Leu Asp Arg Asp
980 985 990
Met Asn Leu Tyr Ala His Phe Gly Phe Gly Pro His Gln Cys Leu Gly
995 10001005
Ile Gly Leu Cys Lys Leu Ala Leu Thr Thr Met Leu Lys Val Val
101010151020
Gly Arg Leu Asp Asn Leu Arg Arg Ala Pro Gly Gly Gln Gly Arg
102510301035
Leu Lys Lys Leu Ser Gly Pro Gly Gly Ile Ala Met Tyr Met Thr
104010451050
Pro Asp Gln Ser Gly Phe Phe Pro Phe Pro Thr Thr Met Lys Ile
105510601065
Gln Trp Asp Gly Asp Leu Pro Pro Leu Lys Thr Glu
107010751080
<210> 65
<211> 1080
<212> PRT
<213> 黑曲霉（Aspergillus niger）
<400> 65
Met Ser Gly Ala Asn Asn His Ser Ile Val Asn Gly Ile Gly Ser Thr
1 5 1015
Ile Ser Gln Val Glu Lys Ala Ile Ser Ala Ser Leu Arg Pro Leu Pro
202530
Thr Ala Thr Gly Asn Gly Thr Tyr Ile Thr Glu Pro Asp Gln Thr Gly
354045
Ile Val Lys Asp Leu Ser His Val Asp Phe Thr Asp Ile Lys Ala Leu
505560
Leu Glu Val Ile Lys Asp Ala Val Thr Gly Gln Pro Val Asp Asp Arg
65707580
His Tyr Ile Met Glu Arg Val Ile Gln Leu Ala Ala Gly Leu Pro Ser
859095
Thr Ser Lys Asn Gly Lys Asp Leu Thr Asn Ala Phe Leu Lys Gln Leu
100 105 110
Trp Asn Asp Leu Glu His Pro Pro Ile Ser Tyr Leu Gly Arg Asp Ser
115 120 125
Ser Tyr Arg Lys Ala Asp Gly Ser Gly Asn Asn Phe Leu Trp Pro His
130 135 140
Ile Gly Ala Ala Gly Ser His Tyr Ala Arg Ser Val Arg Pro Thr Thr
145 150 155 160
Val Gln Ser Pro Ser Leu Pro Asp Pro Ser Thr Leu Phe Ala Ser Leu
165 170 175
Leu Glu Arg Lys Glu Tyr Lys Glu His Pro Asn Lys Ile Ser Ser Val
180 185 190
Leu Phe Tyr Leu Ala Ser Val Ile Ile His Asp Leu Phe Gln Thr Asp
195 200 205
Arg Ser Asp Phe Thr Leu Asn Lys Thr Ser Ser Tyr Leu Asp Leu Ser
210 215 220
Pro Leu Tyr Gly Asn Asn Gln Asp Glu Gln Asp Leu Val Arg Thr Phe
225 230 235 240
Lys Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Ser Lys Arg Val Leu
245 250 255
Gly Phe Pro Pro Gly Val Gly Val Leu Leu Leu Met Phe Asn Arg Phe
260 265 270
His Asn Tyr Val Val Glu Asn Leu Ala Thr Ile Asn Glu Gly Gly Arg
275 280 285
Phe Thr Lys Pro Asp Glu Ser Asp Val Asp Ala Ser Thr Lys Tyr Asp
290 295 300
Asn Asp Leu Phe Gln Thr Gly Arg Leu Val Thr Cys Gly Leu Tyr Ile
305 310 315 320
Asn Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg
325 330 335
Thr Asp Ser Leu Trp Ser Leu Asp Pro Arg Ala Asp Ile Gln Asp Ser
340 345 350
Leu Leu Gly Ser Ala Pro Ala Glu Ala Thr Gly Asn Gln Val Ser Ala
355 360 365
Glu Phe Asn Leu Val Tyr Arg Trp His Ala Cys Ile Ser Gln Arg Asp
370 375 380
Glu Lys Trp Thr Gln Asp Leu Tyr Lys Asp Leu Phe Pro Gly Lys Asp
385 390 395 400
Pro Asn Asn Val Ser Leu Gln Glu Phe Ile Arg Gly Val Ala Lys Trp
405 410 415
Glu Ala Ser Leu Pro Glu Gln Pro Pro Asp Arg Pro Phe Ala Gly Leu
420 425 430
Gln Arg Asn Ala Asp Gly Ala Phe Asp Asp Gly Asp Leu Ala Asn Met
435 440 445
Phe Ala Asp Gly Val Glu Asp Cys Ala Gly Ala Phe Gly Ala Gly Asn
450 455 460
Ile Pro Ser Val Phe Arg Asn Ile Glu Ala Leu Gly Ile Leu Gln Ala
465 470 475 480
Arg Ser Trp Asn Leu Ala Thr Leu Asn Glu Phe Arg Lys Phe Phe Asp
485 490 495
Leu Ala Pro Tyr Lys Thr Phe Glu Glu Ile Asn Pro Asp Pro Tyr Ile
500 505 510
Ala Ala Gln Leu Lys Asn Leu Tyr Asp Glu Pro Asp Leu Val Glu Met
515 520 525
Tyr Pro Gly Val Ile Val Glu Ala Thr Lys Asp Ala Ile Val Pro Gly
530 535 540
Ser Gly Leu Cys Thr Asn Phe Thr Ile Ser Arg Ala Ile Leu Ser Asp
545 550 555 560
Ala Val Ser Leu Val Arg Gly Asp Arg Phe Tyr Thr Val Asp Tyr Thr
565 570 575
Pro Lys His Leu Thr Asn Trp Ala Tyr Asn Glu Ile Gln Pro Gln Asp
580 585 590
Ser Val Asp Gln Gly Gln Val Phe Tyr Lys Leu Val Leu Arg Ala Phe
595 600 605
Pro Asn His Phe Lys Gly Asp Ser Val Tyr Ala His Phe Pro Leu Val
610 615 620
Ile Pro Ser Glu Asn Lys Lys Ile Leu Glu Lys Leu Ser Val Ala Gln
625 630 635 640
Asp Tyr Ser Trp Gly Arg Pro Ser Tyr Thr Pro Leu Pro Gln Phe Ile
645 650 655
Ser Ser Asn Ala Ala Cys Ile Ser Val Leu Asn Asp Gln Glu Ala Phe
660 665 670
Lys Val Thr Trp Gly Ser Lys Ile Glu Phe Leu Met Arg His Asn Asn
675 680 685
His Pro Tyr Gly Arg Asp Phe Met Leu Ser Gly Asp Lys Pro Pro Asn
690 695 700
Ala Ala Ser Arg Arg Met Met Gly Ser Ala Leu Tyr Arg Asp Lys Trp
705 710 715 720
Glu Ser Glu Val Lys Arg Phe Tyr Glu Asp Ile Thr Ile Lys Leu Leu
725 730 735
Arg Gln His Ser Tyr Gln Leu Gly Gly Val Asn Gln Val Asp Ile Val
740 745 750
Arg Asp Val Ala Asn Tyr Ala Gln Val His Phe Cys Ala Asn Val Phe
755 760 765
Ser Leu Pro Leu Lys Thr Glu Ser Asn Pro Arg Gly Ile Phe Ala Glu
770 775 780
Ser Glu Leu Tyr Glu Ile Leu Ala Leu Val Phe Ala Ser Ile Phe Tyr
785 790 795 800
Asp Ala Asp Val Gly Thr Ser Phe Gln Leu Asn Gln Thr Ala Arg Asp
805 810 815
Val Thr Gln Gln Leu Gly Glu Leu Thr Met Ala Asn Val Asp Phe Val
820 825 830
Asn Lys Ala Gly Phe Ile Ala Asn Leu Val Ser Ser Leu His Arg His
835 840 845
Asp Val Leu Ser Glu Tyr Gly Glu His Met Ile Gln Arg Leu Leu His
850 855 860
Ser Asn Val Pro Pro Ala Glu Ile Val Trp Thr His Leu Leu Pro Thr
865 870 875 880
Ala Gly Gly Met Val Ala Asn Gln Ala Gln Leu Phe Ser Gln Cys Leu
885 890 895
Asp Tyr Tyr Leu Ser Asp Glu Gly Ser Ile His Leu Pro Asp Ile Lys
900 905 910
Arg Leu Ala Lys Glu Asn Thr Ser Glu Ala Asp Ala Leu Leu Leu Arg
915 920 925
Tyr Phe Met Glu Gly Ala Arg Leu Arg Ser Ser Val Gly Leu Pro Arg
930 935 940
Leu Val Ala Lys Pro Thr Val Val Asp Asp Gly Gly Ser Lys Tyr Thr
945 950 955 960
Leu Lys Pro Gly Gln Ser Val Leu Cys Asn Leu Val Ser Ala Ser Met
965 970 975
Asp Pro Arg Ser Phe Pro Glu Pro Glu Lys Val Lys Leu Asp Arg Asp
980 985 990
Met Ser Leu Tyr Ala His Phe Gly Phe Gly Pro His Gln Cys Leu Gly
995 10001005
Met Gly Ile Cys Lys Leu Ala Leu Thr Thr Met Leu Arg Val Val
101010151020
Gly Arg Leu Asp Asn Leu Arg Arg Ala Pro Gly Ser Gln Gly Gln
102510301035
Leu Lys Lys Ile Ala Gly Pro Gly Gly Ile Ser Met Tyr Met Thr
104010451050
Ala Asp Gln Ser Ser Tyr Trp Pro Phe Pro Ser Thr Met Lys Ile
105510601065
Gln Trp Asp Gly Asp Leu Pro Ser Leu Ala Thr Asn
107010751080
<210> 66
<211> 1127
<212> PRT
<213> 苹果炭疽病菌（Glomerella cingulate）
<400> 66
Met Ser Gln Gln Ser His Thr Glu Gln Phe Pro Lys Asn Gln Pro Pro
1 5 1015
Leu Ala Glu Arg Leu Ala Ser Ala Arg Gln Leu Val Thr Lys Ala Ile
202530
Ser Ala Val Pro Pro His Pro Glu Pro Leu Pro Ser Pro Asn Ser Asp
354045
Gln Gly Glu Pro Ser Asn Leu Leu Gln Asp Val Lys Lys Leu Gly Phe
505560
Asp Asp Phe Asp Thr Leu Val His Phe Phe Ser Ser Ala Val Glu Gly
65707580
Gln Ile Asn Asp Asn Glu Leu Leu Leu Glu Asn Leu Ile Gln Leu Phe
859095
Ala Lys Leu Pro Gln Asn Ser Val Lys Gly Lys Arg Leu Glu Asn Gly
100 105 110
Leu Ile Asn Gln Leu Trp Asn Gly Ile Asp His Pro Pro Met Thr Thr
115 120 125
Leu Gly Glu Asp His Lys Tyr Arg Ala Ala Asp Gly Ser Gly Asn Ala
130 135 140
Ile His Asp Pro Lys Met Gly Ala Ala Gly Gln Pro Tyr Ala Arg Ser
145 150 155 160
Thr Pro Ala Lys Ser Tyr Gln Asn Pro Asn Gln Pro Glu Pro Glu Met
165 170 175
Ile Phe Asp Met Leu Phe Ala Arg Gly Gly Glu Phe Lys Pro His Pro
180 185 190
Asn Lys Ile Ser Ser Phe Met Phe Tyr Leu Ala Thr Ile Ile Thr His
195 200 205
Asp Ile Phe Gln Thr Lys Asn Gly Thr Ser Pro Ile Asn Gln Thr Ser
210 215 220
Ser Tyr Leu Asp Leu Ala Pro Leu Tyr Gly Arg Asn Gln Ala Glu Gln
225 230 235 240
Asp Leu Met Arg Thr Lys Lys Asn Gly Leu Leu Lys Pro Asp Thr Phe
245 250 255
Ser Ser Lys Arg Val Leu Gly Phe Pro Pro Gly Val Gly Thr Leu Leu
260 265 270
Ile Met Phe Asn Arg Tyr His Asn Tyr Val Ala Thr Asn Leu Ala Thr
275 280 285
Ile Asn Glu Gly Gly Arg Phe Gln Lys Pro Thr Gly Asp Asp Pro Glu
290 295 300
Lys Thr Ala Lys Tyr Asp Asn Asp Leu Phe Gln Thr Ala Arg Leu Ile
305 310 315 320
Thr Cys Gly Leu Tyr Val Ser Ile Ile Leu Arg Asp Tyr Val Arg Thr
325 330 335
Ile Leu Gly Met Asn Arg Thr Ala Ser Ser Trp Ala Leu Asp Pro Arg
340 345 350
Thr Asn Glu Gly Lys Ser Ile Leu Ser Gln Gln Thr Pro Glu Gly Thr
355 360 365
Gly Asn Gln Val Ser Val Glu Phe Asn Leu Ile Tyr Arg Trp His Asn
370 375 380
Thr Ile Ser Pro Lys Asp Glu Gln Trp Thr Lys Asp Val Met Lys Lys
385 390 395 400
Val Leu Gly Lys Asp Pro Thr Glu Met Ser Leu Met Glu Phe Gly His
405 410 415
Ala Met Arg Asp Trp Glu Gln Glu Ile Pro Asp Asp Pro Ala Gln Arg
420 425 430
Gly Phe Met Asp Leu Pro Arg Asn Ala Asp Gly Thr Leu Asn Glu Ala
435 440 445
Asp Leu Ala Lys Ile Phe Lys Glu Ser Val Asp Asp Val Ala Gly Ser
450 455 460
Tyr Gly Ala Asn Arg Ile Pro Glu Val Met Arg Pro Ile Glu Leu Met
465 470 475 480
Gly Ile Met Ala Ser Arg Ser Trp Asn Cys Ala Thr Leu Asn Glu Phe
485 490 495
Arg Glu His Phe Gly Leu Thr Arg His Pro Thr Phe Glu Asp Ile Asn
500 505 510
Pro Asp Lys Glu Val Ala Ala Lys Leu Arg Phe Leu Tyr Gly Ser Pro
515 520 525
Asp Ala Val Glu Leu Tyr Pro Gly Leu Met Ala Glu Lys Ala Lys Pro
530 535 540
Pro Met Ala Pro Gly Ser Gly Leu Cys Gly Asn Phe Thr Met Thr Arg
545 550 555 560
Ala Ile Leu Ser Asp Ala Val Ala Leu Val Arg Gly Asp Arg Phe Tyr
565 570 575
Thr Ile Asp Tyr Thr Pro Lys Asn Leu Thr Asn Trp Gly Phe Asn Gln
580 585 590
Ala Ser Tyr Asp Leu Asn Val Asp Gln Ser His Val Leu Tyr Lys Leu
595 600 605
Val Phe Arg Ala Phe Pro Asn Ser Phe Gln Asn Asn Ser Ile Tyr Ala
610 615 620
His Phe Pro Phe Val Ile Pro Ser Glu Asn Lys Lys Ile Leu Glu Ser
625 630 635 640
Ile Asp Lys Ala Tyr Leu Tyr Thr Trp Asp Glu Pro Lys Thr Lys Thr
645 650 655
Pro Leu Ile Pro Ile Leu Ser His Lys Ala Val Ser Glu Val Leu Tyr
660 665 670
Asn Gln Gln Asp Phe Lys Val Ile Thr Gly Asp Ala Ile Asn His Leu
675 680 685
Val Ala Gln Pro Asn Lys Pro His Tyr Gly Lys Asp Phe Cys Leu Ser
690 695 700
Gly Asp Gly Lys Glu His Ala Lys Asn Arg Thr Leu Val Arg Lys Ser
705 710 715 720
Leu Ile Ser Gly Pro Trp Glu Thr Glu Ile Trp Lys Trp Tyr Thr His
725 730 735
Met Thr Pro Lys Ile Leu Asn Leu Asn Ser Phe Pro Ile Pro Gly Asn
740 745 750
Lys Arg Glu Ile Asp Leu Val Arg Asp Val Ile Asn Val Thr Asn Thr
755 760 765
Arg Phe Asn Ala Ala Leu Phe Cys Met Pro Ile Lys Asn Glu Glu Ser
770 775 780
Pro Trp Gly Val Tyr Thr Asp Gln Glu Leu Tyr Ser Val Leu Gly Ala
785 790 795 800
Leu Phe Gln Ala Val Phe Met Asp Ala Asp Ile Gly Asn Ser Phe Lys
805 810 815
Leu Arg Thr Ile Ala Arg Glu Leu Ala Gln Asp Leu Gly Lys Val Val
820 825 830
Met Leu Ile Ala Gln Thr Ile Lys Lys Ala Gly Leu Ile Thr Asp Ile
835 840 845
Val Ala Lys Ile Arg Glu Gly Glu Ala Ser Leu Pro Thr Tyr Gly Asn
850 855 860
His Leu Ile Glu Arg Met Leu Ser Asp Gly Lys Asp Val Glu Glu Val
865 870 875 880
Val Trp Gly Thr Ile Met Pro Val Ile Thr Ala Asn Val Ala Asn Gln
885 890 895
Ser Gln Val Thr Ser Leu Cys Val Asp Tyr Tyr Leu Asp Lys Glu Gly
900 905 910
Lys Lys His Leu Pro Glu Leu Tyr Arg Leu Ala His Glu Asn Thr Pro
915 920 925
Glu Ala Asp Glu Thr Leu Leu Lys Tyr Met Leu Glu Gly Cys Arg Leu
930 935 940
Arg Gly Pro Val Ala Val Tyr Arg Glu Ala Thr Ser Thr Gln Val Ile
945 950 955 960
Thr Asp Tyr Ser Pro Cys Leu Pro Ser Glu Ser Asp Pro Thr Gly Arg
965 970 975
Asp Pro Ile Thr Asn Pro Asp Ile Glu Gly Thr Lys Arg Glu Val Lys
980 985 990
Ile Pro Arg Gly Tyr Arg Val Val Cys Asn Phe Ala Thr Ala Gly Arg
995 10001005
Asp Pro Ala Ile Phe Glu Asp Pro Asn Glu Val Arg Leu Asp Arg
101010151020
Pro Leu Asp Ser Tyr Val His Phe Gly Leu Gly Pro His Trp Cys
102510301035
Ala Gly Lys Glu Met Ser Arg Val Gly Gln Thr Ser Leu Phe Lys
104010451050
Gln Ile Val Gly Leu Lys Asn Leu Arg Arg Ala Pro Gly Gly Arg
105510601065
Gly Glu Met Lys Asn Phe Pro Ala Ser Pro Trp Asn Gly Gln Val
107010751080
Gly Leu Pro Val Glu Gly Gln Asn Gly Ser Ala Ala His Gln Gln
108510901095
Pro Trp Leu Gly Leu Arg Ala Phe Met Thr Val Asp Gln Ser Ser
110011051110
Leu Trp Pro Ile Pro Thr Thr Met Arg Val Gln Trp Asp Glu
111511201125
<210> 67
<211> 1165
<212> PRT
<213> 小麦全蚀病菌（Gaeumannomyces graminis）
<400> 67
Met Thr Val Ser Thr His His Asp Asp Ser Pro Gly Leu Ser Gly Arg
1 5 1015
Leu Arg Asp Leu Leu His His Val Phe Gly Asn Gln Lys Ser Pro Thr
202530
Val Tyr Pro Asn Ala Pro Gly Asn Ser Ala Lys Pro Val Pro Thr Gly
354045
Leu Ala Asp Asp Ile Asp Lys Leu Gly Phe Lys Asp Ile Asp Thr Leu
505560
Leu Ile Phe Leu Asn Ser Ala Val Lys Gly Val Asn Asp Asp Gln Gln
65707580
Phe Leu Leu Glu Lys Met Ile Gln Leu Leu Ala Lys Leu Pro Pro Ala
859095
Ser Arg Glu Gly Lys Lys Leu Thr Asp Gly Leu Ile Asn Asp Leu Trp
100 105 110
Asp Ser Leu Asp His Pro Pro Val Ala Ser Leu Gly Lys Gly Phe Ser
115 120 125
Phe Arg Glu Pro Asp Gly Ser Asn Asn Asn Ile His Leu Pro Ser Leu
130 135 140
Gly Ala Ala Asn Thr Pro Tyr Ala Arg Ser Thr Lys Pro Leu Val Phe
145 150 155 160
Gln Asn Pro Asn Pro Pro Asp Pro Ala Thr Ile Phe Asp Thr Leu Met
165 170 175
Val Arg Asp Pro Ala Lys Phe Arg Pro His Pro Asn Lys Ile Ser Ser
180 185 190
Met Leu Phe Tyr Leu Ala Thr Ile Ile Thr His Asp Ile Phe Gln Thr
195 200 205
Ser Pro Arg Asp Phe Asn Ile Asn Leu Thr Ser Ser Tyr Leu Asp Leu
210 215 220
Ser Pro Leu Tyr Gly Arg Asn His Asp Glu Gln Met Ala Val Arg Thr
225 230 235 240
Gly Lys Asp Gly Leu Leu Lys Pro Asp Thr Phe Ser Ser Lys Arg Val
245 250 255
Ile Gly Phe Pro Pro Gly Val Gly Ala Phe Leu Ile Met Phe Asn Arg
260 265 270
Phe His Asn Tyr Val Val Thr Gln Leu Ala Lys Ile Asn Glu Gly Gly
275 280 285
Arg Phe Lys Arg Pro Thr Thr Pro Asp Asp Thr Ala Gly Trp Glu Thr
290 295 300
Tyr Asp Asn Ser Leu Phe Gln Thr Gly Arg Leu Ile Thr Cys Gly Leu
305 310 315 320
Tyr Ile Asn Ile Val Leu Gly Asp Tyr Val Arg Thr Ile Leu Asn Leu
325 330 335
Asn Arg Ala Asn Thr Thr Trp Asn Leu Asp Pro Arg Thr Lys Glu Gly
340 345 350
Lys Ser Leu Leu Ser Lys Pro Thr Pro Glu Ala Val Gly Asn Gln Val
355 360 365
Ser Val Glu Phe Asn Leu Ile Tyr Arg Trp His Cys Thr Ile Ser Glu
370 375 380
Arg Asp Asp Lys Trp Thr Thr Asn Ala Met Arg Glu Ala Leu Gly Gly
385 390 395 400
Gln Asp Pro Ala Thr Ala Lys Met Glu Asp Val Met Arg Ala Leu Gly
405 410 415
Met Phe Glu Lys Asn Ile Pro Asp Glu Pro Glu Lys Arg Thr Leu Ala
420 425 430
Gly Leu Thr Arg Gln Ser Asp Gly Ala Phe Asp Asp Thr Glu Leu Val
435 440 445
Lys Ile Leu Gln Glu Ser Ile Glu Asp Val Ala Gly Ala Phe Gly Pro
450 455 460
Asn His Val Pro Ala Cys Met Arg Ala Ile Glu Ile Leu Gly Ile Lys
465 470 475 480
Gln Ser Arg Thr Trp Asn Val Ala Thr Leu Asn Glu Phe Arg Gln Phe
485 490 495
Ile Gly Leu Thr Pro His Asp Ser Phe Tyr His Met Asn Pro Asp Pro
500 505 510
Lys Ile Cys Lys Ile Leu Ala Gln Met Tyr Asp Ser Pro Asp Ala Val
515 520 525
Glu Leu Tyr Pro Gly Ile Met Ala Glu Ala Ala Lys Pro Pro Phe Ser
530 535 540
Pro Gly Ser Gly Leu Cys Pro Pro Tyr Thr Thr Ser Arg Ala Ile Leu
545 550 555 560
Ser Asp Ala Val Ser Leu Val Arg Gly Asp Arg Phe Tyr Thr Val Asp
565 570 575
Tyr Thr Pro Arg Asn Ile Thr Asn Trp Gly Phe Asn Glu Ala Ser Thr
580 585 590
Asp Lys Ala Val Asp Trp Gly His Val Ile Tyr Lys Leu Phe Phe Arg
595 600 605
Ala Phe Pro Asn His Phe Leu Pro Asn Ser Val Tyr Ala His Phe Pro
610 615 620
Phe Val Val Pro Ser Glu Asn Lys Leu Ile Phe Glu Gly Leu Gly Ala
625 630 635 640
Ala Asn Lys Tyr Ser Trp Asp Pro Pro Lys Ala Arg Ala Pro Ile Gln
645 650 655
Phe Ile Arg Ser His Lys Ala Val Leu Glu Val Leu Ser Asn Gln Lys
660 665 670
Asp Tyr Lys Val Thr Trp Gly Pro Ala Ile Lys Met Leu Ser Gly Asp
675 680 685
Pro Ala Thr Ser Phe Ala Leu Ala Gly Asp Glu Pro Ala Asn Ala Ala
690 695 700
Ser Arg His His Val Ile Ala Ala Leu Thr Ala Pro Lys Gln Trp Arg
705 710 715 720
Asp Glu Val Arg Arg Phe Tyr Glu Val Thr Thr Arg Asp Leu Leu Arg
725 730 735
Arg His Gly Ala Pro Val His Gly Val Gly Ala Gly Pro Arg Thr His
740 745 750
Glu Val Asp Val Ile Arg Asp Val Ile Gly Leu Ala His Ala Arg Phe
755 760 765
Met Ala Ser Leu Phe Ser Leu Pro Leu Lys Glu Glu Gly Lys Glu Glu
770 775 780
Gly Ala Tyr Gly Glu His Glu Leu Tyr Arg Ser Leu Val Thr Ile Phe
785 790 795 800
Ala Ala Ile Phe Trp Asp Ser Asp Val Cys Asn Ser Leu Lys Leu His
805 810 815
Gln Ala Ser Lys Ala Ala Ala Asp Lys Met Ser Ala Leu Ile Ala Glu
820 825 830
His Val Arg Glu Met Glu Ala Gly Thr Gly Phe Leu Gly Ala Leu Gly
835 840 845
Lys Leu Lys Asp Leu Ile Thr Gly Asn Asp Val His Ala Asn Gly Asn
850 855 860
Gly Val Tyr Thr Asn Gly Asn Gly Val Tyr Thr Asn Gly Asn Gly Val
865 870 875 880
His Thr Asn Gly Asn Gly Val His Thr Asn Gly Asn Gly Val Pro His
885 890 895
Ala Ala Pro Ser Leu Arg Ser Phe Gly Asp Gln Leu Leu Gln Arg Met
900 905 910
Leu Ser Gln Asp Gly Arg Ser Ile Glu Glu Thr Val Ser Gly Thr Ile
915 920 925
Leu Pro Val Val Met Ala Gly Thr Ala Asn Gln Thr Gln Leu Leu Ala
930 935 940
Gln Cys Leu Asp Tyr Tyr Leu Gly Val Gly Glu Lys His Leu Pro Glu
945 950 955 960
Met Lys Arg Leu Ala Met Leu Asn Thr Ser Glu Ala Asp Glu Lys Leu
965 970 975
Leu Lys Tyr Thr Met Glu Gly Cys Arg Ile Arg Gly Cys Val Ala Leu
980 985 990
Tyr Arg Ala Val Val Thr Asp Gln Ala Val Asp Asp Thr Ile Pro Cys
995 10001005
Ile Pro Asn Lys Asp Asp Pro Thr Phe Ala Arg Pro Leu Ser Asn
101010151020
Pro Gln Val Ala Glu Ser Ala Arg Thr Leu Lys Leu Ser Thr Gly
102510301035
Thr Arg Met Leu Val Asp Leu Thr Thr Ala Ser His Asp Pro Ala
104010451050
Ala Phe Pro Asp Pro Asp Glu Val Arg Leu Asp Arg Pro Leu Glu
105510601065
Ser Tyr Val His Phe Gly Leu Gly Pro His Arg Cys Ala Gly Glu
107010751080
Pro Ile Ser Gln Ile Ala Leu Ser Ser Val Met Lys Val Leu Leu
108510901095
Gln Leu Asp Gly Leu Arg Arg Ala Ala Gly Pro Arg Gly Glu Ile
110011051110
Arg Ser Tyr Pro Ala Ser Gln Trp Pro Gly Gln Ala Gly Arg Pro
111511201125
Pro Arg Asp Pro Ala Trp Ser Gly Leu Arg Thr Phe Thr Ser Ala
113011351140
Asp Gln Ser Ala Phe Ser Pro Leu Ala Thr Thr Met Lys Ile Asn
114511501155
Trp Glu Gly Arg Gly Asp Leu
11601165
<210> 68
<211> 1171
<212> PRT
<213> 稻瘟病菌（Magnaporthe oryzae）
<400> 68
Met Ala Ser Ser Ser Ser Ser Gly Ser Ser Thr Arg Ser Ser Ser Pro
1 5 1015
Ser Asp Pro Pro Ser Ser Phe Phe Gln Lys Leu Gly Ala Phe Leu Gly
202530
Leu Phe Ser Lys Pro Gln Pro Pro Arg Pro Asp Tyr Pro His Ala Pro
354045
Gly Asn Ser Ala Arg Glu Glu Gln Thr Asp Ile Thr Glu Asp Ile Gln
505560
Lys Leu Gly Phe Lys Asp Val Glu Thr Leu Leu Leu Tyr Leu Asn Ser
65707580
Ser Val Lys Gly Val Asn Asp Asp Lys Gln Leu Leu Leu Glu Arg Leu
859095
Ile Gln Leu Leu Ser Lys Leu Pro Pro Thr Ser Thr Asn Gly Lys Lys
100 105 110
Val Thr Asp Gly Leu Ile Thr Gly Leu Trp Glu Ser Leu Asp His Pro
115 120 125
Pro Val Ser Ser Leu Gly Glu Lys Tyr Arg Phe Arg Glu Ala Asp Gly
130 135 140
Ser Asn Asn Asn Ile His Asn Pro Thr Leu Gly Val Ala Gly Ser His
145 150 155 160
Tyr Ala Arg Ser Ala Lys Pro Met Val Tyr Gln Asn Pro Asn Pro Pro
165 170 175
Ala Pro Glu Thr Ile Phe Asp Thr Leu Met Ala Arg Asp Pro Ala Lys
180 185 190
Phe Arg Pro His Pro Asn Gln Ile Ser Ser Val Leu Phe Tyr Phe Ala
195 200 205
Thr Ile Ile Thr His Asp Ile Phe Gln Thr Ser Ser Arg Asp Pro Ser
210 215 220
Ile Asn Leu Thr Ser Ser Tyr Leu Asp Leu Ser Pro Leu Tyr Gly Arg
225 230 235 240
Asn Leu Glu Glu Gln Leu Ser Val Arg Ala Met Lys Asp Gly Leu Leu
245 250 255
Lys Pro Asp Thr Phe Cys Ser Lys Arg Val His Gly Phe Pro Pro Gly
260 265 270
Val Gly Val Leu Leu Ile Met Phe Asn Arg Phe His Asn Tyr Val Val
275 280 285
Thr Ser Leu Ala Lys Ile Asn Glu Gly Asn Arg Phe Lys Lys Pro Val
290 295 300
Gly Asp Asp Thr Ala Ala Trp Glu Lys Tyr Asp Asn Asp Leu Phe Gln
305 310 315 320
Thr Gly Arg Leu Ile Thr Cys Gly Leu Tyr Val Asn Ile Val Leu Val
325 330 335
Asp Tyr Val Arg Thr Ile Leu Asn Leu Asn Arg Val Asp Ser Ser Trp
340 345 350
Ile Leu Asp Pro Arg Thr Glu Glu Gly Lys Ser Leu Leu Ser Lys Pro
355 360 365
Thr Pro Glu Ala Val Gly Asn Gln Val Ser Val Glu Phe Asn Leu Ile
370 375 380
Tyr Arg Trp His Cys Gly Met Ser Gln Arg Asp Asp Lys Trp Thr Thr
385 390 395 400
Asp Met Leu Thr Glu Ala Leu Gly Gly Lys Asp Pro Ala Thr Ala Thr
405 410 415
Leu Pro Glu Phe Phe Gly Ala Leu Gly Arg Phe Glu Ser Ser Phe Pro
420 425 430
Asn Glu Pro Glu Lys Arg Thr Leu Ala Gly Leu Lys Arg Gln Glu Asp
435 440 445
Gly Ser Phe Glu Asp Glu Gly Leu Ile Lys Ile Met Gln Glu Ser Ile
450 455 460
Glu Glu Val Ala Gly Ala Phe Gly Pro Asn His Val Pro Ala Cys Met
465 470 475 480
Arg Ala Ile Glu Ile Leu Gly Met Asn Gln Ala Arg Ser Trp Asn Val
485 490 495
Ala Thr Leu Asn Glu Phe Arg Glu Phe Ile Gly Leu Lys Arg Tyr Asp
500 505 510
Thr Phe Glu Asp Ile Asn Pro Asp Pro Lys Val Ala Asn Leu Leu Ala
515 520 525
Glu Phe Tyr Gly Ser Pro Asp Ala Val Glu Leu Tyr Pro Gly Ile Asn
530 535 540
Ala Glu Ala Pro Lys Pro Val Ile Val Pro Gly Ser Gly Leu Cys Pro
545 550 555 560
Pro Ser Thr Thr Gly Arg Ala Ile Leu Ser Asp Ala Val Thr Leu Val
565 570 575
Arg Gly Asp Arg Phe Phe Thr Val Asp Tyr Thr Pro Arg Asn Leu Thr
580 585 590
Asn Phe Gly Tyr Gln Glu Ala Ala Thr Asp Lys Ser Val Asp Asn Gly
595 600 605
Asn Val Ile Tyr Lys Leu Phe Phe Arg Ala Phe Pro Asn His Tyr Ala
610 615 620
Gln Asn Ser Ile Tyr Ala His Phe Pro Phe Val Ile Pro Ser Glu Asn
625 630 635 640
Lys Lys Ile Met Glu Ser Leu Gly Leu Ala Asp Lys Tyr Ser Trp Gln
645 650 655
Pro Pro Gln Arg Lys Pro Ala Thr Gln Met Ile Arg Ser His Ala Ala
660 665 670
Ala Val Lys Ile Leu Asn Asn Gln Lys Asp Phe Lys Val Val Trp Gly
675 680 685
Glu Ser Ile Gly Phe Leu Thr Lys Phe Pro Thr Gly Glu Asn Pro Gly
690 695 700
Leu Gly Phe Ala Leu Ala Gly Asp Ala Pro Ala Asn Gln Gln Ser Arg
705 710 715 720
Asp Gln Leu Met Lys Cys Ile Phe Ser Pro Lys Ala Trp Glu Asp Glu
725 730 735
Val Arg Gln Phe Cys Glu Ala Thr Thr Trp Asp Leu Leu Arg Arg Tyr
740 745 750
Ser Ala Lys Val Gln Asp Lys Gly Pro His Leu Lys Val His Thr His
755 760 765
Glu Ile Asp Val Ile Arg Asp Val Ile Ser Leu Ala Asn Ala Arg Phe
770 775 780
Phe Ala Ala Val Tyr Ser Leu Pro Leu Lys Thr Glu Asn Gly Asp Asp
785 790 795 800
Gly Val Tyr Ser Asp His Glu Met Tyr Arg Ser Leu Met Leu Ile Phe
805 810 815
Ser Ala Ile Phe Trp Asp Asn Asp Val Ser Lys Ser Phe Lys Leu Arg
820 825 830
Arg Asp Ala Arg Ala Ala Thr Gln Lys Leu Gly Ala Leu Val Glu Lys
835 840 845
His Ile Val Glu Met Gly Ser Leu Phe His Ser Phe Lys His Ser His
850 855 860
Ser Ala Val Ser Asp Lys Thr Asn Gly Leu Ala Asn Gly Gly Ala Asn
865 870 875 880
Gly His Ala Asn Gly Asn Ala Asn Gly His Thr Asn Gly Asn Gly Ile
885 890 895
His Gln Asn Gly Gly Ala Ala Pro Ser Met Leu Arg Ser Tyr Gly Asp
900 905 910
Leu Met Leu Arg Arg Met Ile Glu Ala Tyr Gly Glu Gly Lys Ser Val
915 920 925
Lys Glu Ala Val Tyr Gly Gln Ile Met Pro Ser Ile Ala Ala Gly Thr
930 935 940
Ala Asn Gln Thr Gln Ile Met Ala Gln Cys Leu Asp Tyr Tyr Met Ser
945 950 955 960
Asp Asp Gly Ala Glu His Leu Pro Glu Met Lys Arg Leu Ala Ser Leu
965 970 975
Glu Thr Pro Glu Ala Phe Asn Thr Leu Met Lys Tyr Leu Phe Glu Gly
980 985 990
Ala Arg Ile Arg Asn Thr Thr Ala Val Pro Arg Leu Val Ala Thr Asp
995 10001005
Gln Thr Val Glu Asp Asn Ile Pro Cys Leu Pro Asp Pro Lys Asp
101010151020
Ser Thr Phe Leu Arg Pro Ile Pro Asn Pro Gln Gln Ala Glu Thr
102510301035
Thr Arg Thr Val Lys Leu Ser Arg Gly Ser Met Val Leu Val Asp
104010451050
Leu Thr Val Ala Ala His Asp Ala Thr Ala Phe Pro Asp Pro Glu
105510601065
Lys Val Arg Leu Asp Arg Asp Leu Asp Ser Tyr Thr Phe Phe Gly
107010751080
Leu Gly Pro His Arg Cys Ala Gly Asp Lys Val Val Arg Ile Thr
108510901095
Met Thr Ala Val Phe Lys Val Leu Leu Gln Leu Asp Gly Leu Arg
110011051110
Arg Ala Glu Gly Gly Arg Gly Val Phe Lys Ser Leu Pro Ala Ser
111511201125
Gln Trp Asn Gly Gln Ala Gly Arg Val Ala Gly Glu Lys Pro Gln
113011351140
Trp Ser Gly Leu Arg Thr Tyr Val Asn Ala Asp Glu Ser Ala Phe
114511501155
Ser Gln Thr Pro Met Asn Met Lys Ile Arg Trp Asp Asp
116011651170
<210> 69
<211> 1079
<212> PRT
<213> 草酸青霉（Penicillium oxalicum）
<400> 69
Met Val Gly His His Gly Ser Glu Thr His Gly Glu Lys Ser Pro Leu
1 5 1015
Val Gln Ile Glu Gln Val Phe Lys Ala Ala Leu Arg Pro Leu Pro Thr
202530
Glu Thr Gly Asp Gly Thr Tyr Val Lys Asp Thr Lys Leu Thr Gly Leu
354045
Ala Gln Asp Leu Ser His Val Asp Leu Val Asp Val Lys Thr Leu Ala
505560
Asp Val Ala Lys Asn Ala Ile Thr Gly Glu Ala Met Asn Asp Arg Glu
65707580
Tyr Ile Met Glu Arg Val Ile Gln Leu Ala Ala Gly Leu Pro Thr Thr
859095
Ser Lys Asn Gly Arg Asp Leu Thr Asn Thr Phe Leu Ser Thr Leu Trp
100 105 110
Asn Asp Leu Gln His Pro Pro Thr Ser Tyr Leu Gly Arg Asp Ser Ala
115 120 125
Tyr Arg Gln Ala Asp Gly Ser Gly Asn Asn Pro Phe Trp Pro Asn Ile
130 135 140
Gly Ala Ala Gly Thr Pro Tyr Ala Arg Ser Val Arg Pro Gln Thr Met
145 150 155 160
Gln Pro Gly Ala Leu Pro Glu Pro Glu Thr Leu Phe Asp Ser Leu Leu
165 170 175
Ala Arg Gln Lys Phe Lys Glu His Pro Asn Lys Ile Ser Ser Val Leu
180 185 190
Phe Tyr Leu Ala Ser Ile Ile Ile His Asp Leu Phe Gln Thr Asp Pro
195 200 205
Arg Asn Pro Thr Val Ser Leu Ser Ser Ser Tyr Leu Asp Leu Gly Pro
210 215 220
Leu Tyr Gly Asn Asn Gln Asp Glu Gln Asn Ala Val Arg Thr Phe Lys
225 230 235 240
Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Ser Lys Arg Ile Leu Gly
245 250 255
Phe Pro Pro Gly Val Gly Val Leu Leu Ile Met Phe Asn Arg Phe His
260 265 270
Asn Tyr Val Ala Glu Gln Leu Ala Gln Ile Asn Glu Gly Gly Arg Phe
275 280 285
Thr Lys Pro Ala Glu Ser Asn Thr Lys Ala Tyr Ala Thr Trp Asp Asn
290 295 300
Asp Leu Phe Gln Thr Ser Arg Leu Val Thr Cys Gly Leu Tyr Val Asn
305 310 315 320
Ile Ile Leu Lys Asp Tyr Val Arg Thr Ile Leu Asn Ile Asn Arg Thr
325 330 335
Asp Ser Thr Trp Ser Leu Asp Pro Arg Ala Glu Met Lys Asp Gly Val
340 345 350
Leu Ser His Ala Ala Lys Gln Ala Thr Gly Asn Gln Val Ser Ala Glu
355 360 365
Phe Asn Leu Val Tyr Arg Trp His Ser Cys Ile Ser Ala Arg Asp Gln
370 375 380
Gln Trp Ser Glu Asp Leu Tyr Arg Glu Leu Phe Asn Gly Gln Asp Pro
385 390 395 400
Asn Thr Leu Ser Thr Gln Gln Phe Ile Met Gly Val Gly Arg Trp Glu
405 410 415
Gly Thr Leu Pro Gln Asp Pro Met Glu Arg Pro Phe Ala Lys Leu Gln
420 425 430
Arg Gln Ala Asp Gly Arg Phe Ser Asp Asp Asp Leu Val Arg Phe Phe
435 440 445
Glu Glu Ser Val Glu Asp Val Ala Gly Ala Phe Gly Ala Ser Asn Val
450 455 460
Pro Thr Val Phe Arg Thr Ile Glu Val Leu Gly Ile Lys Gln Ala Arg
465 470 475 480
Ser Trp Asn Leu Ala Thr Leu Asn Glu Phe Arg Ser Phe Phe Asn Leu
485 490 495
Gln Pro Tyr Lys Thr Phe Glu Glu Ile Asn Ser Asp Pro Tyr Ile Ala
500 505 510
Asp Gln Leu Arg His Leu Tyr Asp His Pro Asp Gln Val Glu Leu Tyr
515 520 525
Pro Gly Leu Val Val Glu Asp Ala Lys Glu Pro Met Leu Pro Gly Ser
530 535 540
Gly Leu Cys Thr Asn Tyr Thr Thr Ser Arg Ala Ile Leu Ser Asp Ala
545 550 555 560
Val Thr Leu Val Arg Gly Asp Arg Phe Tyr Thr Val Asp Tyr Thr Pro
565 570 575
Lys His Leu Thr Asn Trp Ala Phe Asn Glu Ile Ser Tyr Asp Asp Ser
580 585 590
Val Asp Asn Gly Ala Ile Phe Tyr Lys Leu Val Leu Arg Ala Phe Pro
595 600 605
Asn His Val Arg Gly Asp Ser Val Tyr Ala His Phe Pro Met Val Val
610 615 620
Pro Ser Glu Asn Lys Lys Ile Leu Thr Ser Leu Gly Lys Ser Glu Lys
625 630 635 640
Tyr Ser Tyr Ser Arg Pro Thr Tyr Thr Pro Pro Pro Arg Met Ile Lys
645 650 655
Ser His Gly Ala Cys Met Ser Ile Leu Ala Asp Lys Glu Thr Phe Lys
660 665 670
Val Thr Trp Gly Gln Lys Leu Glu Phe Ile Leu Ser Arg Asp Gly His
675 680 685
Ser Tyr Gly Gly Asp Phe Met Leu Ser Gly Asp Lys Ala Pro His Ala
690 695 700
Gln Ser Arg Lys Met Ile Gly Asn Ala Leu Tyr Arg Asp Gln Trp Lys
705 710 715 720
Ser Glu Val Arg Ser Phe Tyr Glu Ser Ile Thr Leu Gln Leu Leu Arg
725 730 735
Gln Lys Ser Tyr Lys Leu Ala Gly Val Asn Gln Val Asp Ile Val Arg
740 745 750
Asp Val Ser Asn Leu Ala Gln Ile His Phe Cys Ala Asn Ile Phe Ser
755 760 765
Leu Pro Leu Lys Thr Glu Ser Asn Pro His Gly Val Phe Thr Glu Gln
770 775 780
Glu Leu Tyr Glu Ile Met Ala Leu Val Phe Thr Cys Ile Phe Tyr Asp
785 790 795 800
Val Glu Val Thr Lys Ser Phe Gln Leu Glu Gln Ala Ser Arg Gln Val
805 810 815
Ala Gln Gln Leu Gly Glu Leu Val Met Ala Asn Val Asp Leu Val Ser
820 825 830
Lys Ser Gly Phe Val Ala Asp Ile Ile Ser Arg Leu Arg Arg His Glu
835 840 845
His Leu Thr Asp Tyr Gly Val His Met Ile Gln Arg Leu Leu Asp Ser
850 855 860
Gly Leu Pro Pro Lys Glu Ile Val Trp Thr His Ile Leu Pro Ser Ala
865 870 875 880
Ser Ser Met Val Ala Asn Gln Ala Gln Leu Phe Ile Gln Cys Leu Asp
885 890 895
Phe Tyr Leu Lys Pro Glu Asn Ala His His Leu Ala Asp Ile Gln Arg
900 905 910
Leu Ser Lys Glu Asp Thr Pro Glu Ser Asp Glu Leu Leu Leu Arg Tyr
915 920 925
Phe Met Glu Gly Gly Arg Ile Cys Ser Ser Val Ala Leu Pro Arg Val
930 935 940
Val Ala Lys Ser Thr Val Ile Glu Asp Asn Gly Glu Gln Val Ser Leu
945 950 955 960
Lys Glu Gly Glu Ala Ile Phe Leu Asn Leu Val Ser Ala Ser His Asp
965 970 975
Pro Lys Ala Trp Pro Asp Pro Glu Glu Val Arg Leu Asp Arg Asp Leu
980 985 990
Asn Gln Tyr Ala His Phe Gly Phe Gly Pro His Gln Cys Leu Gly Leu
995 10001005
Gly Val Cys Gln Val Ala Leu Pro Thr Met Leu Arg Val Val Gly
101010151020
Gln Leu Gln Asn Leu Arg Arg Ala Pro Gly Leu Gln Gly Gln Leu
102510301035
Lys Lys Leu Pro Ala Leu Gly Gly Leu Thr Met Tyr Met Asp Ala
104010451050
Asp His Ser Ser Ile Ser Pro Tyr Pro Ser Thr Met Lys Ile Gln
105510601065
Trp Asp Gly Glu Leu Pro Lys Ala Arg Tyr Thr
10701075
<210> 70
<211> 1074
<212> PRT
<213> 产黄青霉（Penicillium chrysogenum）
<400> 70
Met Ala Glu Lys Glu Ser Asn Ser Ser Gly Lys Ile Ala Gln Leu Glu
1 5 1015
Gln Val Val Ala Ala Ala Leu Arg Pro Leu Pro Thr Gln Thr Gly Asp
202530
Gly Ser Tyr Val Gln Glu Pro Thr Val Thr Gly Leu Ala Lys Asp Leu
354045
Leu Asn Phe Asp Leu Lys Asp Ala Lys Thr Leu Ala Asp Met Ala Lys
505560
Thr Ala Val Thr Gly Lys Ala Val Asn Asp Arg Asp Tyr Ile Met Glu
65707580
Arg Val Ile Gln Leu Ala Ser Gly Leu Pro Ser Thr Ser Arg Asn Gly
859095
Lys Glu Leu Thr Asn Thr Phe Leu Thr Gln Leu Trp Gly Asp Leu Glu
100 105 110
His Pro Pro Ile Ser Tyr Leu Gly Arg Asp Ala Ala Tyr Arg Lys Ala
115 120 125
Asp Gly Ser Gly Asn Asn Thr Phe Trp Pro Gln Ile Gly Ala Ala Asn
130 135 140
Thr Pro Tyr Ala Arg Ser Val Arg Pro Lys Thr Met Gln Pro Val Ala
145 150 155 160
Leu Pro Glu Pro Glu Ala Leu Phe Asp Ser Leu Leu Ala Arg Lys Asp
165 170 175
Phe Lys Glu His Pro Asn Lys Ile Ser Ser Val Leu Phe His Leu Ala
180 185 190
Ser Ile Ile Ile His Asp Leu Phe Gln Thr Asp Pro Arg Asp Gln Thr
195 200 205
Lys Ser Leu Thr Ser Ser Tyr Leu Asp Leu Ser Pro Leu Tyr Gly Asn
210 215 220
Asn Gln Lys Glu Gln Asp Thr Val Arg Ala Phe Lys Asp Gly Lys Leu
225 230 235 240
Lys Pro Asp Cys Phe Ser Thr Lys Arg Val Leu Gly Phe Pro Pro Gly
245 250 255
Val Gly Val Ile Leu Ile Met Phe Asn Arg Phe His Asn Ser Val Val
260 265 270
Thr Gln Leu Ala Ala Ile Asn Glu Gly Gly Arg Phe Thr Lys Pro Asp
275 280 285
Glu Ser Asn Ala Glu Ala Tyr Ala Thr Trp Asp Asn Asp Leu Phe Gln
290 295 300
Thr Ala Arg Leu Val Thr Cys Gly Leu Tyr Ile Asn Ile Ile Leu Lys
305 310 315 320
Asp Tyr Val Arg Thr Ile Leu Asn Val Asn Arg Thr Asp Ser Leu Trp
325 330 335
Ser Leu Asp Pro Arg Ala Asp Ile Arg Asp Gly Leu Leu Gly Glu Ala
340 345 350
Pro Ala Gln Ala Thr Gly Asn Gln Val Ser Ala Glu Phe Asn Leu Val
355 360 365
Tyr Arg Trp His Ser Cys Val Ser Ser Arg Asp Glu Lys Trp Ser Glu
370 375 380
Asp Leu Tyr Lys Glu Ile Phe Asp Gly Lys Asp Pro Lys Glu Ile Ser
385 390 395 400
Met Gln Gln Phe Thr Gly Gly Leu Arg Gln Trp Glu Ser Lys Leu Pro
405 410 415
Ala Asp Pro Gln Glu Arg Pro Phe Ala Lys Leu Gln Arg Gln Ala Asp
420 425 430
Gly Lys Phe Asp Asp Asn Asp Leu Val Lys Ile Phe Glu Glu Ser Val
435 440 445
Glu Asp Pro Ala Gly Ala Phe Gly Ala Leu Asn Val Pro Asp Val Phe
450 455 460
Arg Gly Ile Glu Val Leu Gly Ile Lys Gln Ala Arg Ser Trp Asn Leu
465 470 475 480
Ala Thr Leu Asn Glu Phe Arg Gln Tyr Phe Gly Leu Ala Ala Tyr Gln
485 490 495
Thr Phe Glu Glu Ile Asn Pro Asp Pro Tyr Val Ala Asn Gln Leu Lys
500 505 510
His Phe Tyr Asp His Pro Asp Leu Val Glu Leu Tyr Pro Gly Leu Val
515 520 525
Val Glu Glu Thr Lys Gln Ala Met Thr Pro Gly Ser Gly Leu Cys Thr
530 535 540
Asn Phe Thr Thr Ser Arg Ala Ile Leu Ser Asp Ala Val Ala Leu Val
545 550 555 560
Arg Gly Asp Arg Phe Tyr Thr Val Asp Phe Thr Pro Lys His Leu Thr
565 570 575
Asn Trp Ala Phe Asn Glu Ile Asn Asn Asp Val Ser Val Asp Gly Gly
580 585 590
Gln Val Phe Tyr Lys Leu Ile Leu Lys Ala Phe Pro Asn His Phe Arg
595 600 605
Gly Asp Ser Val Tyr Ala His Phe Pro Leu Val Val Pro Asp Glu Asn
610 615 620
Lys Lys Ile Leu Thr Ser Leu Gly Lys Val Lys Thr Tyr Ser Phe Asp
625 630 635 640
Arg Pro Phe Tyr Lys Ala Pro Pro Leu Phe Ile Asn Ser His Ser Ala
645 650 655
Cys Thr Lys Ile Leu Lys Asp Gln Glu Gly Phe Lys Val Val Trp Gly
660 665 670
Glu Lys Ile Gln Phe Leu Met Glu Asn Ser Gly Arg Pro Tyr Gly Arg
675 680 685
Asp Phe Ala Leu Ser Gly Asp Leu Pro Ala Asn Ala Ala Ser Arg Lys
690 695 700
Met Ile Gly Ala Ala Leu His Arg Asp Lys Trp Glu Ser Glu Val Lys
705 710 715 720
Ala Phe Tyr Glu Asp Ile Thr Leu Lys Leu Leu Glu Arg Asn Ser Phe
725 730 735
Lys Val Ala Gly Val Asn Gln Val Asp Ile Val Arg Asp Val Ala Val
740 745 750
Leu Ala Gln Val Asn Phe Cys Ala Asn Val Phe Ser Leu Ser Leu Lys
755 760 765
Thr Glu Ser Asn Pro Arg Gly Val Phe Ser Glu Gln Glu Leu Tyr Gln
770 775 780
Ile Leu Ala Val Ile Phe Ala Ser Ile Phe Tyr Asp Val Asp Val Ser
785 790 795 800
Lys Ser Leu Gln Leu Cys Gln Thr Ala Arg Asn Val Ala Gln Gln Leu
805 810 815
Gly Glu Leu Thr Leu Ala Asn Val Glu Leu Val Ala Lys Thr Gly Phe
820 825 830
Ile Ser Asn Leu Val Asn Arg Leu His Arg His Asp Ile Leu Ser Glu
835 840 845
Tyr Gly Ile His Met Ile Gln Arg Leu Leu Asp Ser Gln Leu Pro Val
850 855 860
Lys Asp Val Val Trp Ser His Ile Leu Pro Thr Ala Gly Ala Leu Val
865 870 875 880
Ala Asn Gln Gly Gln Leu Phe Ser Gln Cys Ile Asp Tyr Tyr Leu Ser
885 890 895
Glu Glu Ala Ala Glu His Leu Ala Glu Ile Gln Arg Leu Ser Arg Glu
900 905 910
Asp Thr Pro Glu Ala Asp Glu Leu Leu Val Arg Tyr Phe Met Glu Gly
915 920 925
Ala Arg Leu Arg Cys Ser Val Ala Leu Pro Arg Phe Ala Thr Lys Pro
930 935 940
Thr Val Val Asp Asp Asn Gly Lys Arg Val Thr Leu Lys Ala Gly Gln
945 950 955 960
Glu Ile Ile Cys Asn Leu Val Val Ala Gly Arg Asp Pro Val Ala Phe
965 970 975
Pro Asp Pro Asp Lys Val Arg Leu Asp Arg Asp Met Ser Leu Tyr Thr
980 985 990
His Phe Gly Phe Gly Pro His Glu Cys Leu Gly Val Lys Met Cys Pro
995 10001005
Leu Ala Leu Ser Thr Met Leu Lys Val Leu Gly Arg Leu Asp Asn
101010151020
Val Arg Arg Ala Pro Gly Pro Gln Gly His Leu Lys Arg Leu Asp
102510301035
Gly Leu Gly Gly Ile Ala Met Tyr Met Asp Ala Glu His Ser Ser
104010451050
Phe Ser Pro Phe Pro Met Thr Met Lys Ile Gln Trp Asp Gly Asp
105510601065
Leu Pro Ala Arg Arg Glu
1070
<210> 71
<211> 1121
<212> PRT
<213> 指状青霉（Penicillium digitatum）
<400> 71
Met Leu Arg Arg Ile Ser Thr Gln Phe Lys Arg Ser Lys Asp Leu Lys
1 5 1015
Asp Ser Lys Asp Phe Lys Asp Ser Asn Gly Glu Asn Thr Glu Asn Ser
202530
Thr Glu Lys Asn Ser Lys Arg Ala Ser Lys Val Ser Pro Thr Arg Lys
354045
Ser Phe Ser Ala Lys Glu Glu His His Val Val Lys Arg Ala Glu Val
505560
Val Ala Val Phe Glu Lys Phe Ala Gln Ala Ile His Ala Ser Lys Glu
65707580
Pro Leu Pro Asn Gln Thr Ser Asp Gly Ala Tyr Leu Lys His Asp Lys
859095
Ser Ser Gly Leu Ile Asn Asp Ile Lys Ser Leu Gly Phe Arg Glu Leu
100 105 110
Asn Thr Val Lys Asp Leu Ile Ala Ser Lys Ala Ser Gly Glu Leu Val
115 120 125
Asp Asp Lys Thr Tyr Leu Met Glu Arg Ile Ile Gln Met Val Ala Asp
130 135 140
Leu Pro Gly Asn Ser Lys Asn Arg Thr Glu Leu Thr Ser Leu Phe Leu
145 150 155 160
Asp Glu Leu Trp Asn Ser Ile Pro His Pro Pro Leu Ser Tyr Met Gly
165 170 175
Asp Glu Tyr Lys Tyr Arg Ser Ala Asp Gly Ser Asn Asn Asn Pro Thr
180 185 190
Leu Pro Trp Leu Gly Ala Ala Asn Thr Ala Tyr Cys Arg Thr Ile Pro
195 200 205
Pro Leu Thr Ile Gln Pro Ser Gly Leu Pro Asp Ala Gly Leu Ile Phe
210 215 220
Asp Thr Leu Phe Ala Arg Gln Glu Phe Thr Pro His Pro Asn Lys Val
225 230 235 240
Ser Ser Val Phe Phe Asp Trp Ala Ser Leu Ile Ile His Asp Ile Phe
245 250 255
Gln Thr Asp Tyr Arg Gln Gln His Leu Asn Lys Thr Ser Ala Tyr Leu
260 265 270
Asp Leu Ser Ile Leu Tyr Gly Asp Val Gln Glu Gln Gln Asp Leu Ile
275 280 285
Arg Ser His Gln Asp Gly Lys Leu Lys Pro Asp Cys Phe Ser Glu Gly
290 295 300
Arg Leu Gln Ala Leu Pro Ala Ala Cys Gly Val Leu Leu Val Met Leu
305 310 315 320
Asn Arg Phe His Asn His Val Val Thr Gln Leu Ala Glu Ile Asn Glu
325 330 335
Asn Gly Arg Phe Ser Lys Pro Arg Pro Gly Leu Ser Glu Glu Asp Ala
340 345 350
Lys Lys Val Trp Thr Lys Arg Asp Glu Asp Leu Phe Gln Thr Gly Arg
355 360 365
Leu Ile Thr Cys Gly Leu Tyr Ile Asn Ile Thr Leu Tyr Asp Tyr Leu
370 375 380
Arg Thr Ile Val Asn Leu Asn Arg Thr Asn Ser Thr Trp Cys Leu Asp
385 390 395 400
Pro Arg Ala Gln Val Glu Lys Ala Gly Ala Thr Pro Ser Gly Leu Gly
405 410 415
Asn Gln Cys Ser Val Glu Phe Asn Leu Ala Tyr Arg Trp His Ser Thr
420 425 430
Ile Ser Gln Gly Asp Glu Lys Trp Ile Glu Gln Ile Tyr His Asp Leu
435 440 445
Met Gly Lys Pro Ala Glu Glu Val Thr Met Pro Glu Leu Leu Met Gly
450 455 460
Leu Lys Lys Val Glu Gly Leu Leu Asp Thr Asp Pro Ala Lys Arg Thr
465 470 475 480
Phe Ala Arg Leu Gln Arg Asn Glu Asp Gly Phe Phe Asn Asp Gly Glu
485 490 495
Leu Val Asn Ile Leu Thr His Ala Thr Glu Asp Val Ala Ser Ser Phe
500 505 510
Gly Pro Arg Asn Val Pro Lys Ala Met Arg Ser Ile Glu Ile Leu Gly
515 520 525
Ile Glu Ala Ser Arg Arg Trp Asn Val Gly Ser Leu Asn Glu Phe Arg
530 535 540
Lys His Phe Gly Leu Lys Pro Tyr Glu Thr Phe Glu Glu Val Asn Ser
545 550 555 560
Asn Pro Glu Ile Ser Asn Thr Leu Arg His Leu Tyr Asp His Pro Asp
565 570 575
Phe Ile Glu Leu Tyr Pro Gly Ile Val Thr Glu Glu Ala Lys Glu Pro
580 585 590
Met Ile Pro Gly Val Gly Ile Ala Pro Thr Tyr Thr Ile Ser Arg Ala
595 600 605
Val Leu Ser Asp Ala Val Ala Leu Val Arg Gly Asp Arg His Tyr Thr
610 615 620
Ile Asp Tyr Asn Pro Arg Asn Leu Thr Asn Trp Gly Tyr Asn Glu Cys
625 630 635 640
Arg Tyr Asp Leu Asn Ile Asn Gln Gly Cys Ile Phe Tyr Lys Leu Ala
645 650 655
Thr Arg Ala Phe Pro Asn His Tyr Arg Pro Asp Ser Ile Tyr Ala His
660 665 670
Tyr Pro Met Thr Ile Pro Ser Glu Asn Arg Asn Ile Met Lys Asp Leu
675 680 685
Gly Arg Glu Gln Asp Tyr Ser Trp Asp Lys Pro Ala Phe Ile Glu Pro
690 695 700
Arg Val Asn Leu Ala Ser His Gln Asn Ala Lys Leu Leu Leu Glu Asn
705 710 715 720
Gln Arg Asp Phe Arg Pro Ser Trp Ala Arg Ser Val Ser Glu Leu Phe
725 730 735
Gly Lys Gly Glu Phe Asp Thr Lys Gln Arg Glu Ala Ile Gly Lys Ala
740 745 750
Leu Asn Thr Glu Asp Phe Pro Lys Leu Val Lys Thr Phe Tyr Glu Asp
755 760 765
Ile Thr Glu Arg Leu Ile Val Glu Lys Gly Gly Gln Leu Gly Lys Ile
770 775 780
Asn Gln Ile Asp Ile Thr Arg Asp Val Gly Asn Leu Ala His Val His
785 790 795 800
Phe Ala Ser Thr Ile Phe Gly Ile Pro Leu Lys Thr Glu Gln Ser Pro
805 810 815
Gln Gly Leu Phe Thr Glu His Glu Met Tyr Met Ile Leu Ser Thr Ile
820 825 830
Phe Ser Ala Leu Phe Phe Asp Val Asp Ala Pro Arg Ser Tyr Ala Leu
835 840 845
Asn His Ala Ala Ser Ala Val Ser Thr Gln Leu Gly Gln Val Val Glu
850 855 860
Ala Thr Val Lys Ala Asp Thr Asn Ser Gly Leu Phe Ser Gly Ile Met
865 870 875 880
Asp Ser Phe Arg Pro His Asp Asn Ala Leu Arg Glu Phe Gly Thr Glu
885 890 895
Ala Val Arg Arg Met Lys Glu Ala Gly Ser Ser Ala Ser Asp Ile Thr
900 905 910
Trp Ser Ala Ile Ile Pro Thr Ile Val Gly Leu Val Pro Ser Gln Gly
915 920 925
Gln Val Phe Thr Gln Ile Ile Glu Phe Tyr Thr Ala Pro Glu Asn Gln
930 935 940
Ala His Leu Ala Ala Ile His Ser Leu Thr Lys Thr Asp Ser Ala Glu
945 950 955 960
Ser Asp Glu Lys Leu His Arg Tyr Cys Leu Glu Ala Ile Arg Leu Asn
965 970 975
Gly Thr Phe Gly Ala Phe Arg Glu Ala Lys Glu Ala Val Thr Ile Glu
980 985 990
Glu Asp Gly Lys Val Tyr Ala Val Gln Pro Gly Gln Gln Val Phe Ala
995 10001005
Ser Phe Asn Gln Ala Asn His Asp Pro Ser Val Phe Pro Glu Pro
101010151020
Tyr Gln Val Asn Leu His Arg Pro Leu Asn Ser Tyr Ile Asn His
102510301035
Gly Gln Gly Pro Thr Thr Gly Phe Gly Glu Gln Ile Thr Lys Ile
104010451050
Ala Leu Val Ala Met Leu Arg Val Val Gly Arg Leu Gln Gly Leu
105510601065
Arg Arg Ala Ala Gly Ala Gln Gly Gln Leu Gln Lys Ile Pro Gln
107010751080
Glu Gly Gly Tyr Tyr Val Tyr Leu Arg Gly Asp Gly Ala Ala Tyr
108510901095
Cys Pro Phe Pro Met Ser Leu Lys Leu His Trp Asp Gly Pro Val
110011051110
Gly Gln Lys Lys Thr Pro Ser Ser
11151120
<210> 72
<211> 805
<212> PRT
<213> 深海单细胞蓝细菌（Acaryochloris marina）
<400> 72
Met Asp Ser Arg Asp Pro Arg Thr Glu His Val Asp Asp Glu Phe Lys
1 5 1015
Lys Leu Ile Ser Asn Ile Ala Arg Ala Phe Gly Arg Thr Ala Gln Ile
202530
Lys Gly Arg Arg Ala Thr His Ser Tyr Gly Thr Val Ala Lys Gly Val
354045
Leu Lys Val Leu Asp Thr Leu Asp Ile Pro Gln His Gln Ile Phe Ser
505560
Ala Gly Lys Gln Tyr Pro Val Leu Leu Arg His Ala Asn Ile Lys Gly
65707580
Phe Arg Asp Asp Ala Ile Leu Asp Gly Arg Gly Ala Thr Val Arg Val
859095
Leu Ala Gly Asp Ala Gln Ala Pro Leu Ser Asp Leu Asn Leu Asp Glu
100 105 110
Gly Ile Val Asp Ile Leu Met Ser Thr Gly Arg Ser Phe Ile Leu Ala
115 120 125
Glu Ala Leu Ser Phe Ala Arg Trp Ala Ala Gly Pro Met Lys Ser Arg
130 135 140
Ala Ala Met Leu Gln Glu Phe Pro Lys Ile Ala Pro Ile Phe His Glu
145 150 155 160
Ile Ile Arg Asp Pro Asp Ser Tyr Thr Gln Leu His Tyr Tyr Ser Glu
165 170 175
Thr Thr Tyr Ser Phe Thr Ser Leu Asn Gln Gln Ser Phe Phe Leu Arg
180 185 190
Tyr Arg Leu Val Asn Arg Gln Asn Pro Ser Ala Asp Thr Gly Trp Leu
195 200 205
Lys Pro Glu Glu Val Lys Leu Pro Leu Asp Tyr Leu Pro Arg Val Ala
210 215 220
Ser Asp Thr Arg Pro Glu Thr Tyr Leu Gln Asp Asp Phe Arg Gln Gln
225 230 235 240
Val Arg Ser Thr Gly Val Ser Tyr Leu Leu Gln Ile Gln Leu Gln Pro
245 250 255
Val Ser Asp Asp Ala Ala Met Asn Glu Thr Ala Lys Asp Cys Thr Ile
260 265 270
Pro Trp Glu Glu Glu Asp His Pro Phe His Asp Val Ala Val Leu Asp
275 280 285
Leu Gly Ser Ile Leu Pro Asp Glu Leu Ala Glu Ala Leu Glu Phe Asn
290 295 300
Pro Tyr Asn Ala Pro Pro Glu Leu Ser Leu Ile Leu Ala Lys Thr Ala
305 310 315 320
Arg Glu Thr Ala Ser Val Asn His Leu Arg Ser Val Val Tyr Gln Ile
325 330 335
Ser Ala Asn Met Arg Lys Tyr Gln Thr Pro Ser Ser Ser Leu Val Asp
340 345 350
Trp Gly Ser Gly His Gln Pro Ser Leu Pro Glu Gln Tyr Pro Tyr Gly
355 360 365
Thr Gly Lys Thr Pro Ser Phe Asp Asn Thr Lys Pro Leu Pro Ala Arg
370 375 380
Val Lys Pro Lys Pro Arg Tyr Trp Ala Asn Phe Gly Leu Lys Leu Ile
385 390 395 400
Pro Asn Gln Gln Leu Asp Pro Asp Leu Pro Glu Leu Gly Ile Thr Gly
405 410 415
Ala Leu Asp Leu Met Gly Thr Ser Val Val Ser Tyr Met Pro Pro Asn
420 425 430
Leu Thr Arg Thr Arg Leu Asp Lys Phe Ser Asp Asp Phe Phe Val Glu
435 440 445
Arg Arg Leu Asn Gly Phe Asn Pro Gly Lys Leu Asn Arg Val Thr Gly
450 455 460
His Ala Trp Gln Tyr Gln Val Cys Tyr Asp Cys Ser Lys His Gln Val
465 470 475 480
Glu Pro Ala Gly Ile Leu Pro Thr Lys Ile Thr Ala Arg Phe Asn Phe
485 490 495
Cys Gly Gln Tyr Leu His Pro His Ser Ile Gln Phe Thr Leu Asn Gly
500 505 510
Gln Thr Glu Thr Gln Gln Pro Gly Asp Glu Asn Trp Glu Trp Ser Lys
515 520 525
Arg Leu Phe Arg Cys Ala Glu Phe Val Phe Gln Glu Ala Gln Ser His
530 535 540
Leu Gly Arg Thr His Met Asn Leu Asp Gln Tyr Ala Met Ala Tyr Tyr
545 550 555 560
Arg Asn Val Val Asn Asn Pro Ile Arg Leu Leu Leu Glu Pro His Leu
565 570 575
Glu Gly Leu Leu Ser Ile Asn Lys Leu Gly Ala Asn Leu Ile Ser Gly
580 585 590
Pro Thr Gly Phe Ile Pro Glu Ala Ser Ser Leu Thr Pro Glu Ser Val
595 600 605
Asp Asp Val Leu Lys Asp Glu Ile Ser His Leu Ser Tyr His Trp Thr
610 615 620
Pro His Arg Gln Thr Leu Pro Asp Arg Val Leu Asn Asn His Tyr Asp
625 630 635 640
Pro Ala Ala Ile Ala Met Trp Asn Leu Leu Thr Gln Tyr Val Arg Glu
645 650 655
Phe Phe Glu Asp His Gln Ala Gly Met Glu Glu Tyr Trp Ser Glu Ile
660 665 670
Gln Ala Met Ser His Asp Leu Val Thr His Ser Ile Leu Lys Pro Glu
675 680 685
Leu Gly Thr Leu Ala Val Gln Asn Asn Ala Asp Leu Gln Gln Leu Cys
690 695 700
Val Tyr Val Ile Phe Leu Ser Ser Phe Phe His Ser Trp Val Asn Asn
705 710 715 720
Lys Gln Tyr Glu Asp Gly Gly Asp Val Ser Tyr Ser Thr Ile Gly Leu
725 730 735
Trp Asp Thr Arg His Pro Lys Tyr Asp Pro Leu Arg Val Ala Glu Arg
740 745 750
Glu Ala Lys Gln Val Thr Leu Leu Trp Thr Leu Ser His Val Arg Tyr
755 760 765
Asn Pro Ile Met Asp Val Gly Pro Thr Ala Leu Lys Asn Leu Leu Trp
770 775 780
Gln Gln Arg Gln His Ile Glu Pro Gly Ile Pro Leu Ala Asn Leu Met
785 790 795 800
Met Ser Thr Asn Ile
805
<210> 73
<211> 822
<212> PRT
<213> 念珠藻属(Nostoc sp.)
<400> 73
Met Thr Asn Glu Ile Gln Asn Pro Val Asp His Leu Val Gly Asp Pro
1 5 1015
Arg Val Arg Asp Ile Glu Pro Lys Phe Ser Lys Ile Ile Ser Asp Ile
202530
Ala Arg Val Phe Gly Gln Met Ala Gln Leu Lys Gly Arg Arg Ala Thr
354045
His Ser Phe Gly Thr Val Ala Lys Gly Val Leu Glu Val Val Lys Gln
505560
Pro Asp Ile Pro Pro His Arg Leu Leu Glu Ala Gly Lys Lys Phe Pro
65707580
Val Leu Leu Arg His Ala Asn Ile Lys Gly Phe Arg Asp Asp Ala Ile
859095
Leu Asp Gly Arg Gly Ala Thr Leu Arg Ile Leu Asn Gly Pro Ala Asp
100 105 110
Ala Pro Ile Thr Ala Leu Asp Leu His Thr Pro Ile Leu Asp Val Leu
115 120 125
Met Ser Thr Gly Arg Cys Phe Ile Leu Pro Asp Ala Ala Ser Phe Ser
130 135 140
Arg Trp Val Ala Ser Pro Leu Gln Asp Arg Ala Lys Leu Leu Val Glu
145 150 155 160
Phe Pro Lys Ile Thr Pro Ile Phe Ala Glu Ile Ile Arg Asn Pro Asp
165 170 175
Ser Tyr Thr Lys Leu His Tyr Tyr Ser Glu Thr Thr Tyr Leu Phe Ile
180 185 190
Gly Leu Asp Gly Lys Gln Tyr Tyr Leu Arg Tyr Arg Leu Ile Asn Ser
195 200 205
Asp Arg Ser Ala Asp Thr Gly Phe Ile Asn Ser Ala Asp Leu Arg Leu
210 215 220
Pro Leu Asp Tyr Leu Pro Arg Leu Glu Asp Asp Thr Arg Pro Glu Thr
225 230 235 240
Tyr Leu Gln Asn Asp Tyr Gln Gln Lys Val Lys Asn Gly Gly Val Lys
245 250 255
Tyr Ile Leu Gln Phe Gln Leu Arg Ala Val Ser Asp Asn Gln Ala Ala
260 265 270
Asn Glu Glu Ala Lys Asp Cys Thr Ile Pro Trp Asp Glu Thr Gln Tyr
275 280 285
Pro Cys Lys Asp Val Ala Val Ile Ser Leu Thr Glu Ile Val Pro Ser
290 295 300
Glu Leu Ala Glu Pro Leu Glu Phe Asn Pro Tyr His Ala Pro Pro Asp
305 310 315 320
Leu Ser Leu Ile Leu Ala His Ser Ile Asn Glu Thr Ala Ser Val Asn
325 330 335
His Leu Arg Ser Val Val Tyr Gln Ile Ser Ala Asn Met Arg Lys Phe
340 345 350
Gln Gln Pro Ser Ala Glu Leu Val Asp Trp Gly Ile Lys Gly Asn Gln
355 360 365
Pro Asp Pro Lys Gln Val Tyr Thr Tyr Tyr Gly Gln Ile Gly Gln Asp
370 375 380
Ile Pro Arg Tyr Asp Phe Arg Arg Pro Leu Pro Asp Arg Val Lys Pro
385 390 395 400
Lys Pro Arg Tyr Ile Ala Asn Phe Gly Leu His Leu Phe Pro Ala Arg
405 410 415
Pro Leu Gly Ser Ile Pro Met Leu Gly Ile Val Gly Val Ala Glu Thr
420 425 430
Met Gln Ala Leu Gly Gln Thr Ala Pro Gln Trp Met Pro Ala Asn Leu
435 440 445
Thr Arg Thr Arg Pro Asp Lys Tyr Glu Asp Gln Phe Phe Val Glu Arg
450 455 460
Arg Leu Asn Gly Phe Asn Pro Gly Lys Phe Asn Arg Val His Thr Phe
465 470 475 480
Glu Pro Trp Gln Tyr Thr Ile Arg Tyr Asp Cys Arg Lys Tyr Lys Val
485 490 495
Glu Glu Ala Gly Ile Leu Pro Ala Glu Ile Glu Ala Arg Phe Lys Phe
500 505 510
Glu Asp His Asn Leu His Leu His Ser Ile Lys Phe Ile Leu Asn Gly
515 520 525
Lys Thr Glu Thr His Lys Pro Gly Asp Ala Asp Trp Glu Trp Ser Lys
530 535 540
Arg Leu Phe Arg Thr Ala Glu Phe Val Phe Gln Glu Ile Gln Ser His
545 550 555 560
Leu Gly Arg Thr His Met Asn Leu Asp Gln Tyr Ala Met Ala Tyr Tyr
565 570 575
Arg Asn Val Val Asn Asn Pro Ile Arg Leu Leu Leu Glu Pro His Phe
580 585 590
Asp Gly Leu Leu Asn Ile Asn Ser Leu Gly Ala Ala Leu Ile Leu Gly
595 600 605
Ala Thr Gly Phe Ile Pro Glu Ala Ser Ser Leu Ser Pro Glu Glu Val
610 615 620
Asp Ile Val Leu Lys Asp Glu Ile Ser Arg Leu Ser Tyr Arg Asn Trp
625 630 635 640
Ser Pro His Ile Gln Ala Leu Lys Asp Glu Val Gln Asn Asn His Phe
645 650 655
Asp Arg Ala Ala Leu Ser Val Trp Glu Ala Ile Glu Glu Tyr Val Ser
660 665 670
Glu Phe Phe Gln Lys Gln Glu Ala Gly Ile Lys Ala Tyr Trp Ser Glu
675 680 685
Ile Gly Gly Met Ser Glu Asp Leu Val Lys His Ser Val Leu Gly Gln
690 695 700
Lys Ser Gln Ser Leu Ala Ile Ala Asn Ile Asn Asp Leu Lys Gln Leu
705 710 715 720
Cys Ile Tyr Val Ile Tyr Leu Ser Ser Phe Phe His Ser Trp Val Asn
725 730 735
Asn Lys Gln Tyr Asp Asp Gly Gly Asp Pro Ala Tyr Ala Ser Ile Gly
740 745 750
Leu Trp Asp Gly His Asn Pro Gln Tyr Asn Pro Ile Thr Val Ala Glu
755 760 765
Lys His Ala Arg Gln Val Thr Leu Leu Trp Thr Leu Ser Ser Val Arg
770 775 780
Tyr Asn Pro Val Met Glu Val Gly Pro Pro Ala Leu Lys Asp Arg Leu
785 790 795 800
Trp Lys Arg Arg Lys Ile Ile Gln Pro Gly Ile Pro Val Glu Ser Ile
805 810 815
Met Met Ser Thr Asn Ile
820
<210> 74
<211> 371
<212> PRT
<213> 茶树菇（Agrocybe aegerita）
<400> 74
Met Lys Tyr Phe Pro Leu Phe Pro Thr Leu Val Phe Ala Ala Arg Val
1 5 1015
Val Ala Phe Pro Ala Tyr Ala Ser Leu Ala Gly Leu Ser Gln Gln Glu
202530
Leu Asp Ala Ile Ile Pro Thr Leu Glu Ala Arg Glu Pro Gly Leu Pro
354045
Pro Gly Pro Leu Glu Asn Ser Ser Ala Lys Leu Val Asn Asp Glu Ala
505560
His Pro Trp Lys Pro Leu Arg Pro Gly Asp Ile Arg Gly Pro Cys Pro
65707580
Gly Leu Asn Thr Leu Ala Ser His Gly Tyr Leu Pro Arg Asn Gly Val
859095
Ala Thr Pro Val Gln Ile Ile Asn Ala Val Gln Glu Gly Leu Asn Phe
100 105 110
Asp Asn Gln Ala Ala Val Phe Ala Thr Tyr Ala Ala His Leu Val Asp
115 120 125
Gly Asn Leu Ile Thr Asp Leu Leu Ser Ile Gly Arg Lys Thr Arg Leu
130 135 140
Thr Gly Pro Asp Pro Pro Pro Pro Ala Ser Val Gly Gly Leu Asn Glu
145 150 155 160
His Gly Thr Phe Glu Gly Asp Ala Ser Met Thr Arg Gly Asp Ala Phe
165 170 175
Phe Gly Asn Asn His Asp Phe Asn Glu Thr Leu Phe Glu Gln Leu Val
180 185 190
Asp Tyr Ser Asn Arg Phe Gly Gly Gly Lys Tyr Asn Leu Thr Val Ala
195 200 205
Gly Glu Leu Arg Phe Lys Arg Ile Gln Asp Ser Ile Ala Thr Asn Pro
210 215 220
Asn Phe Ser Phe Val Asp Phe Arg Phe Phe Thr Ala Tyr Gly Glu Thr
225 230 235 240
Thr Phe Pro Ala Asn Leu Phe Val Asp Gly Arg Arg Asp Asp Gly Gln
245 250 255
Leu Asp Met Asp Ala Ala Arg Ser Phe Phe Gln Phe Ser Arg Met Pro
260 265 270
Asp Asp Phe Phe Arg Ala Pro Ser Pro Arg Ser Gly Thr Gly Val Glu
275 280 285
Val Val Ile Gln Ala His Pro Met Gln Pro Gly Arg Asn Val Gly Lys
290 295 300
Ile Asn Ser Tyr Thr Val Asp Pro Thr Ser Ser Asp Phe Ser Thr Pro
305 310 315 320
Cys Leu Met Tyr Glu Lys Phe Val Asn Ile Thr Val Lys Ser Leu Tyr
325 330 335
Pro Asn Pro Thr Val Gln Leu Arg Lys Ala Leu Asn Thr Asn Leu Asp
340 345 350
Phe Phe Phe Gln Gly Val Ala Ala Gly Cys Thr Gln Val Phe Pro Tyr
355 360 365
Gly Arg Asp
370
<210> 75
<211> 417
<212> PRT
<213> 枯草芽孢杆菌(Bacillus subtilis)
<400> 75
Met Asn Glu Gln Ile Pro His Asp Lys Ser Leu Asp Asn Ser Leu Thr
1 5 1015
Leu Leu Lys Glu Gly Tyr Leu Phe Ile Lys Asn Arg Thr Glu Arg Tyr
202530
Asn Ser Asp Leu Phe Gln Ala Arg Leu Leu Gly Lys Asn Phe Ile Cys
354045
Met Thr Gly Ala Glu Ala Ala Lys Val Phe Tyr Asp Thr Asp Arg Phe
505560
Gln Arg Gln Asn Ala Leu Pro Lys Arg Val Gln Lys Ser Leu Phe Gly
65707580
Val Asn Ala Ile Gln Gly Met Asp Gly Ser Ala His Ile His Arg Lys
859095
Met Leu Phe Leu Ser Leu Met Thr Pro Pro His Gln Lys Arg Leu Ala
100 105 110
Glu Leu Met Thr Glu Glu Trp Lys Ala Ala Val Thr Arg Trp Glu Lys
115 120 125
Ala Asp Glu Val Val Leu Phe Glu Glu Ala Lys Glu Ile Leu Cys Arg
130 135 140
Val Ala Cys Tyr Trp Ala Gly Val Pro Leu Lys Glu Thr Glu Val Lys
145 150 155 160
Glu Arg Ala Asp Asp Phe Ile Asp Met Val Asp Ala Phe Gly Ala Val
165 170 175
Gly Pro Arg His Trp Lys Gly Arg Arg Ala Arg Pro Arg Ala Glu Glu
180 185 190
Trp Ile Glu Val Met Ile Glu Asp Ala Arg Ala Gly Leu Leu Lys Thr
195 200 205
Thr Ser Gly Thr Ala Leu His Glu Met Ala Phe His Thr Gln Glu Asp
210 215 220
Gly Ser Gln Leu Asp Ser Arg Met Ala Ala Ile Glu Leu Ile Asn Val
225 230 235 240
Leu Arg Pro Ile Val Ala Ile Ser Tyr Phe Leu Val Phe Ser Ala Leu
245 250 255
Ala Leu His Glu His Pro Lys Tyr Lys Glu Trp Leu Arg Ser Gly Asn
260 265 270
Ser Arg Glu Arg Glu Met Phe Val Gln Glu Val Arg Arg Tyr Tyr Pro
275 280 285
Phe Gly Pro Phe Leu Gly Ala Leu Val Lys Lys Asp Phe Val Trp Asn
290 295 300
Asn Cys Glu Phe Lys Lys Gly Thr Ser Val Leu Leu Asp Leu Tyr Gly
305 310 315 320
Thr Asn His Asp Pro Arg Leu Trp Asp His Pro Asp Glu Phe Arg Pro
325 330 335
Glu Arg Phe Ala Glu Arg Glu Glu Asn Leu Phe Asp Met Ile Pro Gln
340 345 350
Gly Gly Gly His Ala Glu Lys Gly His Arg Cys Pro Gly Glu Gly Ile
355 360 365
Thr Ile Glu Val Met Lys Ala Ser Leu Asp Phe Leu Val His Gln Ile
370 375 380
Glu Tyr Asp Val Pro Glu Gln Ser Leu His Tyr Ser Leu Ala Arg Met
385 390 395 400
Pro Ser Leu Pro Glu Ser Gly Phe Val Met Ser Gly Ile Arg Arg Lys
405 410 415
Ser
<210> 76
<211> 415
<212> PRT
<213> 少动鞘氨醇单胞菌（Sphingomonas paucimobilis）
<400> 76
Met Pro Lys Thr Pro His Thr Lys Gly Pro Asp Glu Thr Leu Ser Leu
1 5 1015
Leu Ala Asp Pro Tyr Arg Phe Ile Ser Arg Gln Cys Gln Arg Leu Gly
202530
Ala Asn Ala Phe Glu Ser Arg Phe Leu Leu Lys Lys Thr Asn Cys Leu
354045
Lys Gly Ala Lys Ala Ala Glu Ile Phe Tyr Asp Thr Thr Arg Phe Glu
505560
Arg Glu Gly Ala Met Pro Val Ala Ile Gln Lys Thr Leu Leu Gly Gln
65707580
Gly Gly Val Gln Gly Leu Asp Gly Glu Thr His Arg His Arg Lys Gln
859095
Met Phe Met Gly Leu Met Thr Pro Glu Arg Val Arg Ala Leu Ala Gln
100 105 110
Leu Phe Glu Ala Glu Trp Arg Arg Ala Val Pro Gly Trp Thr Arg Lys
115 120 125
Gly Glu Ile Val Phe Tyr Asp Glu Leu His Glu Pro Leu Thr Arg Ala
130 135 140
Val Cys Ala Trp Ala Gly Val Pro Leu Pro Asp Asp Glu Ala Gly Asn
145 150 155 160
Arg Ala Gly Glu Leu Arg Ala Leu Phe Asp Ala Ala Gly Ser Ala Ser
165 170 175
Pro Arg His Leu Trp Ser Arg Leu Ala Arg Arg Arg Val Asp Ala Trp
180 185 190
Ala Lys Arg Ile Ile Glu Gly Ile Arg Ala Gly Ser Ile Gly Ser Gly
195 200 205
Ser Gly Thr Ala Ala Tyr Ala Ile Ala Trp His Arg Asp Arg His Asp
210 215 220
Asp Leu Leu Ser Pro His Val Ala Ala Val Glu Leu Val Asn Val Leu
225 230 235 240
Arg Pro Thr Val Ala Ile Ala Val Tyr Ile Thr Phe Val Ala His Ala
245 250 255
Leu Gln Thr Cys Ser Gly Ile Arg Ala Ala Leu Val Gln Gln Pro Asp
260 265 270
Tyr Ala Glu Leu Phe Val Gln Glu Val Arg Arg Phe Tyr Pro Phe Phe
275 280 285
Pro Ala Val Val Ala Arg Ala Ser Gln Asp Phe Glu Trp Glu Gly Met
290 295 300
Ala Phe Pro Glu Gly Arg Gln Val Val Leu Asp Leu Tyr Gly Ser Asn
305 310 315 320
His Asp Ala Ala Thr Trp Ala Asp Pro Gln Glu Phe Arg Pro Glu Arg
325 330 335
Phe Arg Ala Trp Asp Glu Asp Ser Phe Asn Phe Ile Pro Gln Gly Gly
340 345 350
Gly Asp His Tyr Leu Gly His Arg Cys Pro Gly Glu Trp Ile Val Leu
355 360 365
Ala Ile Met Lys Val Ala Ala His Leu Leu Val Asn Ala Met Arg Tyr
370 375 380
Asp Val Pro Asp Gln Asp Leu Ser Ile Asp Phe Ala Arg Leu Pro Ala
385 390 395 400
Leu Pro Lys Ser Gly Phe Val Met Arg Asn Val His Ile Gly Gly
405 410 415
<210> 77
<211> 418
<212> PRT
<213> 丙酮丁醇梭菌（Clostridium acetobutylicum）
<400> 77
Met Leu Leu Lys Glu Asn Thr Ala Lys Asp Lys Gly Ile Asp Ser Thr
1 5 1015
Leu Asp Leu Leu Lys Glu Gly Tyr Leu Phe Ile Lys Asn Arg Ala Asp
202530
His Tyr Gln Ser Asp Leu Phe Glu Thr Arg Leu Met Gly Gln Arg Ile
354045
Ile Cys Met Thr Gly Glu Glu Ala Ala Arg Ile Phe Tyr Asp Ser Asp
505560
Lys Phe Lys Arg Gln Gly Ala Ala Pro Lys Arg Val Gln Glu Thr Leu
65707580
Leu Gly Glu Asn Ala Ile Gln Thr Leu Asp Gly Glu Ser His Leu His
859095
Arg Lys Lys Leu Phe Met Leu Leu Thr Asn Gln Val Gln Gln Lys Arg
100 105 110
Leu Ala Glu Leu Thr Thr Glu Lys Trp Glu Ala Ser Ala Ser Lys Trp
115 120 125
His Thr Lys Ser Ile Val Leu Phe Asn Glu Ala Asn Glu Ile Leu Cys
130 135 140
Gln Val Ala Cys His Trp Ala Gly Val Pro Leu Met Glu Ser Asp Ile
145 150 155 160
Lys Asn Arg Ala Glu Asp Phe Ser Ser Met Ile Asp Ser Phe Gly Ala
165 170 175
Val Gly Pro Arg His Trp Lys Gly Lys Lys Ala Arg Asn Thr Ile Glu
180 185 190
Ala Trp Ile Lys Glu Ile Ile Glu Asn Val Arg Ser Gly Arg Ile Arg
195 200 205
Ala Glu Glu Gly Ser Pro Leu His Glu Ile Ala Phe Tyr Ile Asp Val
210 215 220
Asn Gly Gln Gln Met Pro Ala Glu Met Ala Ala Ile Glu Leu Ile Asn
225 230 235 240
Ile Leu Arg Pro Ile Val Ala Ile Ser Thr Phe Ile Thr Phe Ser Ala
245 250 255
Leu Ala Leu Tyr Glu His Ser Glu Tyr Arg Glu Lys Leu Gln Ser Lys
260 265 270
Asp Ile Arg Tyr Leu Glu Met Phe Thr Gln Glu Val Arg Arg Tyr Tyr
275 280 285
Pro Phe Ala Pro Phe Val Gly Ala Arg Val Arg Lys Asp Phe Leu Trp
290 295 300
Asn Asn Cys Glu Phe Lys Lys Glu Met Leu Val Leu Leu Asp Ile Tyr
305 310 315 320
Gly Thr Asn His Asp Ser Arg Ile Trp Gln Lys Pro Tyr Glu Phe Ile
325 330 335
Pro Asp Arg Phe Arg Ser Tyr Lys Gly Asn Leu Phe Asp Phe Ile Pro
340 345 350
Gln Gly Gly Gly Asp Pro Ser Ser Thr His Arg Cys Pro Gly Glu Gly
355 360 365
Ile Thr Leu Glu Ile Met Lys Thr Ser Leu Asp Phe Leu Ser Thr Lys
370 375 380
Ile Asp Phe Thr Val Pro Asp Gln Asp Leu Ser Tyr Ser Leu Ser Lys
385 390 395 400
Ile Pro Thr Leu Pro Lys Ser Gly Phe Ile Ile Asp Asn Ile Asn Leu
405 410 415
Lys Leu
<210> 78
<211> 646
<212> PRT
<213> 脑膜败血伊丽莎白菌（Elizabethkingia meningoseptica）
<400> 78
Met Asn Pro Ile Thr Ser Lys Phe Asp Lys Val Leu Asn Ala Ser Ser
1 5 1015
Glu Tyr Gly His Val Asn His Glu Pro Asp Ser Ser Lys Glu Gln Gln
202530
Arg Asn Thr Pro Gln Lys Ser Met Pro Phe Ser Asp Gln Ile Gly Asn
354045
Tyr Gln Arg Asn Lys Gly Ile Pro Val Gln Ser Tyr Asp Asn Ser Lys
505560
Ile Tyr Ile Ile Gly Ser Gly Ile Ala Gly Met Ser Ala Ala Tyr Tyr
65707580
Phe Ile Arg Asp Gly His Val Pro Ala Lys Asn Ile Thr Phe Leu Glu
859095
Gln Leu His Ile Asp Gly Gly Ser Leu Asp Gly Ala Gly Asn Pro Thr
100 105 110
Asp Gly Tyr Ile Ile Arg Gly Gly Arg Glu Met Asp Met Thr Tyr Glu
115 120 125
Asn Leu Trp Asp Met Phe Gln Asp Ile Pro Ala Leu Glu Met Pro Ala
130 135 140
Pro Tyr Ser Val Leu Asp Glu Tyr Arg Leu Ile Asn Asp Asn Asp Ser
145 150 155 160
Asn Tyr Ser Lys Ala Arg Leu Ile Asn Asn Lys Gly Glu Ile Lys Asp
165 170 175
Phe Ser Lys Phe Gly Leu Asn Lys Met Asp Gln Leu Ala Ile Ile Arg
180 185 190
Leu Leu Leu Lys Asn Lys Glu Glu Leu Asp Asp Leu Thr Ile Glu Asp
195 200 205
Tyr Phe Ser Glu Ser Phe Leu Lys Ser Asn Phe Trp Thr Phe Trp Arg
210 215 220
Thr Met Phe Ala Phe Glu Asn Trp His Ser Leu Leu Glu Leu Lys Leu
225 230 235 240
Tyr Met His Arg Phe Leu His Ala Ile Asp Gly Leu Asn Asp Leu Ser
245 250 255
Ser Leu Val Phe Pro Lys Tyr Asn Gln Tyr Asp Thr Phe Val Thr Pro
260 265 270
Leu Arg Lys Phe Leu Gln Glu Lys Gly Val Asn Ile His Leu Asn Thr
275 280 285
Leu Val Lys Asp Leu Asp Ile His Ile Asn Thr Glu Gly Lys Val Val
290 295 300
Glu Gly Ile Ile Thr Glu Gln Asp Gly Lys Glu Val Lys Ile Pro Val
305 310 315 320
Gly Lys Asn Asp Tyr Val Ile Val Thr Thr Gly Ser Met Thr Glu Asp
325 330 335
Thr Phe Tyr Gly Asn Asn Lys Thr Ala Pro Ile Ile Gly Ile Asp Asn
340 345 350
Ser Thr Ser Gly Gln Ser Ala Gly Trp Lys Leu Trp Lys Asn Leu Ala
355 360 365
Ala Lys Ser Glu Ile Phe Gly Lys Pro Glu Lys Phe Cys Ser Asn Ile
370 375 380
Glu Lys Ser Ala Trp Glu Ser Ala Thr Leu Thr Cys Lys Pro Ser Ala
385 390 395 400
Leu Ile Asp Lys Leu Lys Glu Tyr Ser Val Asn Asp Pro Tyr Ser Gly
405 410 415
Lys Thr Val Thr Gly Gly Ile Ile Thr Ile Thr Asp Ser Asn Trp Leu
420 425 430
Met Ser Phe Thr Cys Asn Arg Gln Pro His Phe Pro Glu Gln Pro Asp
435 440 445
Asp Val Leu Val Leu Trp Val Tyr Ala Leu Phe Met Asp Lys Glu Gly
450 455 460
Asn Tyr Ile Lys Lys Thr Met Leu Glu Cys Thr Gly Asp Glu Ile Leu
465 470 475 480
Ala Glu Leu Cys Tyr His Leu Gly Ile Glu Asp Gln Leu Glu Asn Val
485 490 495
Gln Lys Asn Thr Ile Val Arg Thr Ala Phe Met Pro Tyr Ile Thr Ser
500 505 510
Met Phe Met Pro Arg Ala Lys Gly Asp Arg Pro Arg Val Val Pro Glu
515 520 525
Gly Cys Lys Asn Leu Gly Leu Val Gly Gln Phe Val Glu Thr Asn Asn
530 535 540
Asp Val Val Phe Thr Met Glu Ser Ser Val Arg Thr Ala Arg Ile Ala
545 550 555 560
Val Tyr Lys Leu Leu Asn Leu Asn Lys Gln Val Pro Asp Ile Asn Pro
565 570 575
Leu Gln Tyr Asp Ile Arg His Leu Leu Lys Ala Ala Lys Thr Leu Asn
580 585 590
Asp Asp Lys Pro Phe Val Gly Glu Gly Leu Leu Arg Lys Val Leu Lys
595 600 605
Gly Thr Tyr Phe Glu His Val Leu Pro Ala Gly Ala Ala Glu Glu Glu
610 615 620
Glu His Glu Ser Phe Ile Ala Glu His Val Asn Lys Phe Arg Glu Trp
625 630 635 640
Val Lys Gly Ile Arg Gly
645
<210> 79
<211> 589
<212> PRT
<213> 溶酪大球菌（Macrococcus caseolyticus）
<400> 79
Met Tyr Tyr Ser Asn Gly Asn Tyr Glu Ala Phe Ala Arg Pro Lys Lys
1 5 1015
Pro Glu Gly Val Asp Asn Lys Ser Ala Tyr Leu Val Gly Ser Gly Leu
202530
Ala Ser Leu Ala Ala Ala Ser Phe Leu Ile Arg Asp Gly Gln Met Lys
354045
Gly Glu Asn Ile His Ile Leu Glu Glu Leu Asp Leu Pro Gly Gly Ser
505560
Leu Asp Gly Ile Leu Asn Pro Glu Arg Gly Tyr Ile Met Arg Gly Gly
65707580
Arg Glu Met Glu Asn His Phe Glu Cys Leu Trp Asp Leu Phe Arg Ser
859095
Val Pro Ser Leu Glu Val Glu Asp Ala Ser Val Leu Asp Glu Phe Tyr
100 105 110
Trp Leu Asn Lys Glu Asp Pro Asn Tyr Ser Lys Cys Arg Val Ile Glu
115 120 125
Asn Arg Gly Gln Arg Leu Glu Ser Asp Gly Lys Met Thr Leu Thr Lys
130 135 140
Lys Ala Asn Lys Glu Ile Ile Gln Leu Cys Leu Met Lys Glu Glu Gln
145 150 155 160
Leu Asn Asp Val Lys Ile Ser Asp Val Phe Ser Lys Asp Phe Leu Asp
165 170 175
Ser Asn Phe Trp Ile Tyr Trp Lys Thr Met Phe Ala Phe Glu Pro Trp
180 185 190
His Ser Ala Met Glu Met Arg Arg Tyr Leu Met Arg Phe Ile His His
195 200 205
Ile Gly Gly Leu Ala Asp Phe Ser Ala Leu Lys Phe Thr Lys Phe Asn
210 215 220
Gln Phe Glu Ser Leu Val Met Pro Leu Ile Glu His Leu Lys Ala Lys
225 230 235 240
Asn Val Thr Phe Glu Tyr Gly Val Thr Val Lys Asn Ile Gln Val Glu
245 250 255
Cys Ser Lys Glu Ser Lys Val Ala Lys Ala Ile Asp Ile Val Arg Arg
260 265 270
Gly Asn Glu Glu Ser Ile Pro Leu Thr Glu Asn Asp Leu Val Phe Val
275 280 285
Thr Asn Gly Ser Ile Thr Glu Ser Thr Thr Tyr Gly Asp Asn Asp Thr
290 295 300
Pro Ala Pro Pro Thr Ser Lys Pro Gly Gly Ala Trp Gln Leu Trp Glu
305 310 315 320
Asn Leu Ser Thr Gln Cys Glu Glu Phe Gly Asn Pro Ala Lys Phe Tyr
325 330 335
Lys Asp Leu Pro Glu Lys Ser Trp Phe Val Ser Ala Thr Ala Thr Thr
340 345 350
Asn Asn Lys Glu Val Ile Asp Tyr Ile Gln Lys Ile Cys Lys Arg Asp
355 360 365
Pro Leu Ser Gly Arg Thr Val Thr Gly Gly Ile Val Thr Val Asp Asp
370 375 380
Ser Asn Trp Gln Leu Ser Phe Thr Leu Asn Arg Gln Gln Gln Phe Lys
385 390 395 400
Asn Gln Pro Asp Asp Gln Val Ser Val Trp Ile Tyr Ala Leu Tyr Ser
405 410 415
Asp Glu Arg Gly Glu Arg Thr Asn Lys Thr Ile Val Glu Cys Ser Gly
420 425 430
Lys Glu Ile Cys Glu Glu Trp Leu Tyr His Met Gly Val Pro Glu Glu
435 440 445
Lys Ile Ser Ala Leu Ala Ala Glu Cys Asn Thr Ile Pro Ser Tyr Met
450 455 460
Pro Tyr Ile Thr Ala Tyr Phe Met Pro Arg Lys Glu Gly Asp Arg Pro
465 470 475 480
Leu Val Val Pro His Gly Ser Lys Asn Ile Ala Phe Ile Gly Asn Phe
485 490 495
Ala Glu Thr Glu Arg Asp Thr Val Phe Thr Thr Glu Tyr Ser Val Arg
500 505 510
Thr Ala Met Glu Ala Val Tyr Lys Leu Leu Glu Val Asp Arg Gly Val
515 520 525
Pro Glu Val Phe Ala Ser Val Tyr Asp Val Arg Ile Leu Leu His Ala
530 535 540
Leu Ser Val Leu Asn Asp Gly Lys Lys Leu Asp Glu Ile Asp Met Pro
545 550 555 560
Phe Tyr Glu Arg Leu Val Glu Lys Arg Leu Leu Lys Lys Ala Ser Gly
565 570 575
Thr Phe Ile Glu Glu Leu Leu Glu Glu Ala Asn Leu Ile
580 585
<210> 80
<211> 590
<212> PRT
<213> 酿脓链球菌（Streptococcus pyogenes）
<400> 80
Met Tyr Tyr Thr Ser Gly Asn Tyr Glu Ala Phe Ala Thr Pro Arg Lys
1 5 1015
Pro Glu Gly Val Asp Gln Lys Ser Ala Tyr Ile Val Gly Thr Gly Leu
202530
Ala Gly Leu Ala Ala Ala Val Phe Leu Ile Arg Asp Gly His Met Ala
354045
Gly Glu Arg Ile His Leu Phe Glu Glu Leu Pro Leu Ala Gly Gly Ser
505560
Leu Asp Gly Ile Glu Lys Pro His Leu Gly Phe Val Thr Arg Gly Gly
65707580
Arg Glu Met Glu Asn His Phe Glu Cys Met Trp Asp Met Tyr Arg Ser
859095
Ile Pro Ser Leu Glu Ile Pro Gly Ala Ser Tyr Leu Asp Glu Phe Tyr
100 105 110
Trp Leu Asp Lys Asp Asp Pro Asn Ser Ser Asn Cys Arg Leu Ile His
115 120 125
Lys Arg Gly Asn Arg Val Asp Asp Asp Gly Gln Tyr Thr Leu Gly Lys
130 135 140
Gln Ser Lys Glu Leu Ile His Leu Ile Met Lys Thr Glu Glu Ser Leu
145 150 155 160
Gly Asp Gln Thr Ile Glu Glu Phe Phe Ser Glu Asp Phe Phe Lys Ser
165 170 175
Asn Phe Trp Val Tyr Trp Ala Thr Met Phe Ala Phe Glu Lys Trp His
180 185 190
Ser Ala Val Glu Met Arg Arg Tyr Ala Met Arg Phe Ile His His Ile
195 200 205
Asp Gly Leu Pro Asp Phe Thr Ser Leu Lys Phe Asn Lys Tyr Asn Gln
210 215 220
Tyr Asp Ser Met Val Lys Pro Ile Ile Ala Tyr Leu Glu Ser His Asp
225 230 235 240
Val Asp Ile Gln Phe Asp Thr Lys Val Thr Asp Ile Gln Val Glu Gln
245 250 255
Thr Ala Gly Lys Lys Val Ala Lys Thr Ile His Met Thr Val Ser Gly
260 265 270
Glu Ala Lys Ala Ile Glu Leu Thr Pro Asp Asp Leu Val Phe Val Thr
275 280 285
Asn Gly Ser Ile Thr Glu Ser Ser Thr Tyr Gly Ser His His Glu Val
290 295 300
Ala Lys Pro Thr Lys Ala Leu Gly Gly Ser Trp Asn Leu Trp Glu Asn
305 310 315 320
Leu Ala Ala Gln Ser Asp Asp Phe Gly His Pro Lys Val Phe Tyr Gln
325 330 335
Asp Leu Pro Ala Glu Ser Trp Phe Val Ser Ala Thr Ala Thr Ile Lys
340 345 350
His Pro Ala Ile Glu Pro Tyr Ile Glu Arg Leu Thr His Arg Asp Leu
355 360 365
His Asp Gly Lys Val Asn Thr Gly Gly Ile Ile Thr Ile Thr Asp Ser
370 375 380
Asn Trp Met Met Ser Phe Ala Ile His Arg Gln Pro His Phe Lys Glu
385 390 395 400
Gln Lys Glu Asn Glu Thr Thr Val Trp Ile Tyr Gly Leu Tyr Ser Asn
405 410 415
Ser Glu Gly Asn Tyr Val His Lys Lys Ile Glu Glu Cys Thr Gly Gln
420 425 430
Glu Ile Thr Glu Glu Trp Leu Tyr His Leu Gly Val Pro Val Asp Lys
435 440 445
Ile Lys Asp Leu Ala Ser Gln Glu Tyr Ile Asn Thr Val Pro Val Tyr
450 455 460
Met Pro Tyr Ile Thr Ser Tyr Phe Met Pro Arg Val Lys Gly Asp Arg
465 470 475 480
Pro Lys Val Ile Pro Asp Gly Ser Val Asn Leu Ala Phe Ile Gly Asn
485 490 495
Phe Ala Glu Ser Pro Ser Arg Asp Thr Val Phe Thr Thr Glu Tyr Ser
500 505 510
Ile Arg Thr Ala Met Glu Ala Val Tyr Ser Phe Leu Asn Gly Glu Arg
515 520 525
Gly Ile Pro Gln Gly Phe Asn Ser Ala Tyr Asp Ile Arg Glu Leu Leu
530 535 540
Lys Ala Phe Tyr Tyr Leu Asn Asp Lys Lys Ala Ile Lys Asp Met Asp
545 550 555 560
Leu Pro Ile Pro Ala Leu Ile Glu Lys Ile Gly His Lys Lys Ile Lys
565 570 575
Asp Thr Phe Ile Glu Glu Leu Leu Lys Asp Ala Asn Leu Met
580 585 590
